P10911 (MCF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proto-oncogene DBL

Alternative name(s):
Proto-oncogene MCF-2

Cleaved into the following 2 chains:

1. MCF2-transforming protein
2. DBL-transforming protein

Gene names

Name:	MCF2
Synonyms:	DBL

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	925 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42.
Subunit structure	Interacts with an array of inositol phospholipids such as phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4-phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P). May interact with CCPG1. Ref.12 Ref.13
Subcellular location	Cytoplasm Ref.12. Isoform 1: Membrane Ref.12. Isoform 3: Membrane. Note: Colocalizes with CDC42 to plasma membrane. Ref.12
Tissue specificity	Isoform 1 is expressed only in brain. Isoform 3 is expressed in heart, kidney, spleen, liver and testis. Isoform 4 is expressed in brain, heart, kidney, testis, placenta, stomach and peripheral blood. The protein is detectable in brain, heart, kidney, intestine, muscle, lung and testis. Ref.3
Domain	The CRAL-TRIO domain is involved in interaction with inositol phospholipids. Ref.9 Ref.10 Ref.12 The DH domain is essential for transforming activity and directly catalyzes GDP-GTP exchange activity. It may interact with CCPG1. Ref.9 Ref.10 Ref.12
Post-translational modification	Phosphorylation by TNK2 enhances guanine nucleotide exchange factor (GEF) activity toward Rho family proteins.
Involvement in disease	Note=MCF2 and DBL represent two activated versions of the same proto-oncogene.
Sequence similarities	Belongs to the MCF2 family. Contains 1 CRAL-TRIO domain. Contains 1 DH (DBL-homology) domain. Contains 1 PH domain. Contains 1 spectrin repeat.
Sequence caution	The sequence AAA52172.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Disease	Proto-oncogene
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Traceable author statement. Source: Reactome induction of apoptosis by extracellular signals Traceable author statement. Source: Reactome nerve growth factor receptor signaling pathway Traceable author statement. Source: Reactome regulation of Rho protein signal transduction Inferred from electronic annotation. Source: InterPro small GTPase mediated signal transduction Traceable author statement. Source: Reactome
Cellular component	cytoskeleton Traceable author statement. Source: ProtInc cytosol Traceable author statement. Source: Reactome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane fraction Traceable author statement. Source: ProtInc
Molecular function	Rho guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NME1	P15531	13	EBI-1914514,EBI-741141

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P10911-1)

Also known as: Var.1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P10911-2)

Also known as: Var.2;

The sequence of this isoform differs from the canonical sequence as follows:
     842-860: KQQGAFISTEETELEHTST → DLCRRWLSYIDEATMSNGK
     861-925: Missing.

Isoform 3 (identifier: P10911-3)

Also known as: Var.3;

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: MAEANPRRGKMRFRRNA → MQDIAFLSGG...SLKISLQKIS

Isoform 4 (identifier: P10911-4)

Also known as: Var.4;

The sequence of this isoform differs from the canonical sequence as follows:
     454-454: Q → QVGVGYSFFQACKLFSK

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 925	925	Proto-oncogene DBL		PRO_0000030432
	Chain	398 – 925	528	MCF2-transforming protein		PRO_0000030433
	Chain	498 – 925	428	DBL-transforming protein		PRO_0000030434
Regions
	Domain	1 – 88	88	CRAL-TRIO
	Repeat	221 – 322	102	Spectrin
	Domain	495 – 675	181	DH
	Domain	687 – 809	123	PH
Natural variations
	Alternative sequence	1 – 17	17	MAEAN…FRRNA → MQDIAFLSGGRGKDNAWIIT FPENCNFRCIPEEVIAKVLT YLTSIARQNGSDSRFTIILD RRLDTWSSLKISLQKIS in isoform 3.		VSP_008150
	Alternative sequence	454	1	Q → QVGVGYSFFQACKLFSK in isoform 4.		VSP_008153
	Alternative sequence	842 – 860	19	KQQGA…EHTST → DLCRRWLSYIDEATMSNGK in isoform 2.		VSP_008151
	Alternative sequence	861 – 925	65	Missing in isoform 2.		VSP_008152
Experimental info
	Mutagenesis	640 – 646	7	LLLKELL → IIIRDII: Transformation capability reduced; no stimulation of GDP dissociation.
	Sequence conflict	54	1	L → P Ref.1
	Sequence conflict	54	1	L → P Ref.3
	Sequence conflict	634	1	R → Q Ref.7
	Sequence conflict	886	1	A → V Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Var.1) [UniParc]. Last modified March 7, 2006. Version 3. Checksum: 96233C7AFC85D637	FASTA	925	107,673
	10 20 30 40 50 60 MAEANPRRGK MRFRRNAASF PGNLHLVLVL RPTSFLQRTF TDIGFWFSQE DFMLKLPVVM 70 80 90 100 110 120 LSSVSDLLTY IDDKQLTPEL GGTLQYCHSE WIIFRNAIEN FALTVKEMAQ MLQSFGTELA 130 140 150 160 170 180 ETELPDDIPS IEEILAIRAE RYHLLKNDIT AVTKEGKILL TNLEVPDTEG AVSSRLECHR 190 200 210 220 230 240 QISGDWQTIN KLLTQVHDME TAFDGFWEKH QLKMEQYLQL WKFEQDFQQL VTEVEFLLNQ 250 260 270 280 290 300 QAELADVTGT IAQVKQKIKK LENLDENSQE LLSKAQFVIL HGHKLAANHH YALDLICQRC 310 320 330 340 350 360 NELRYLSDIL VNEIKAKRIQ LSRTFKMHKL LQQARQCCDE GECLLANQEI DKFQSKEDAQ 370 380 390 400 410 420 KALQDIENFL EMALPFINYE PETLQYEFDV ILSPELKVQM KTIQLKLENI RSIFENQQAG 430 440 450 460 470 480 FRNLADKHVR PIQFVVPTPE NLVTSGTPFF SSKQGKKTWR QNQSNLKIEV VPDCQEKRSS 490 500 510 520 530 540 GPSSSLDNGN SLDVLKNHVL NELIQTERVY VRELYTVLLG YRAEMDNPEM FDLMPPLLRN 550 560 570 580 590 600 KKDILFGNMA EIYEFHNDIF LSSLENCAHA PERVGPCFLE RKDDFQMYAK YCQNKPRSET 610 620 630 640 650 660 IWRKYSECAF FQECQRKLKH RLRLDSYLLK PVQRITKYQL LLKELLKYSK DCEGSALLKK 670 680 690 700 710 720 ALDAMLDLLK SVNDSMHQIA INGYIGNLNE LGKMIMQGGF SVWIGHKKGA TKMKDLARFK 730 740 750 760 770 780 PMQRHLFLYE KAIVFCKRRV ESGEGSDRYP SYSFKHCWKM DEVGITEYVK GDNRKFEIWY 790 800 810 820 830 840 GEKEEVYIVQ ASNVDVKMTW LKEIRNILLK QQELLTVKKR KQQDQLTERD KFQISLQQND 850 860 870 880 890 900 EKQQGAFIST EETELEHTST VVEVCEAIAS VQAEANTVWT EASQSAEISE EPAEWSSNYF 910 920 YPTYDENEEE NRPLMRPVSE MALLY
	Isoform 2 (Var.2) [UniParc]. Checksum: CCD14CA833425027	FASTA	860	100,409
	10 20 30 40 50 60 MAEANPRRGK MRFRRNAASF PGNLHLVLVL RPTSFLQRTF TDIGFWFSQE DFMLKLPVVM 70 80 90 100 110 120 LSSVSDLLTY IDDKQLTPEL GGTLQYCHSE WIIFRNAIEN FALTVKEMAQ MLQSFGTELA 130 140 150 160 170 180 ETELPDDIPS IEEILAIRAE RYHLLKNDIT AVTKEGKILL TNLEVPDTEG AVSSRLECHR 190 200 210 220 230 240 QISGDWQTIN KLLTQVHDME TAFDGFWEKH QLKMEQYLQL WKFEQDFQQL VTEVEFLLNQ 250 260 270 280 290 300 QAELADVTGT IAQVKQKIKK LENLDENSQE LLSKAQFVIL HGHKLAANHH YALDLICQRC 310 320 330 340 350 360 NELRYLSDIL VNEIKAKRIQ LSRTFKMHKL LQQARQCCDE GECLLANQEI DKFQSKEDAQ 370 380 390 400 410 420 KALQDIENFL EMALPFINYE PETLQYEFDV ILSPELKVQM KTIQLKLENI RSIFENQQAG 430 440 450 460 470 480 FRNLADKHVR PIQFVVPTPE NLVTSGTPFF SSKQGKKTWR QNQSNLKIEV VPDCQEKRSS 490 500 510 520 530 540 GPSSSLDNGN SLDVLKNHVL NELIQTERVY VRELYTVLLG YRAEMDNPEM FDLMPPLLRN 550 560 570 580 590 600 KKDILFGNMA EIYEFHNDIF LSSLENCAHA PERVGPCFLE RKDDFQMYAK YCQNKPRSET 610 620 630 640 650 660 IWRKYSECAF FQECQRKLKH RLRLDSYLLK PVQRITKYQL LLKELLKYSK DCEGSALLKK 670 680 690 700 710 720 ALDAMLDLLK SVNDSMHQIA INGYIGNLNE LGKMIMQGGF SVWIGHKKGA TKMKDLARFK 730 740 750 760 770 780 PMQRHLFLYE KAIVFCKRRV ESGEGSDRYP SYSFKHCWKM DEVGITEYVK GDNRKFEIWY 790 800 810 820 830 840 GEKEEVYIVQ ASNVDVKMTW LKEIRNILLK QQELLTVKKR KQQDQLTERD KFQISLQQND 850 860 EDLCRRWLSY IDEATMSNGK
	Isoform 3 (Var.3) [UniParc]. Checksum: 8D0C81505593C040	FASTA	985	114,376
	10 20 30 40 50 60 MQDIAFLSGG RGKDNAWIIT FPENCNFRCI PEEVIAKVLT YLTSIARQNG SDSRFTIILD 70 80 90 100 110 120 RRLDTWSSLK ISLQKISASF PGNLHLVLVL RPTSFLQRTF TDIGFWFSQE DFMLKLPVVM 130 140 150 160 170 180 LSSVSDLLTY IDDKQLTPEL GGTLQYCHSE WIIFRNAIEN FALTVKEMAQ MLQSFGTELA 190 200 210 220 230 240 ETELPDDIPS IEEILAIRAE RYHLLKNDIT AVTKEGKILL TNLEVPDTEG AVSSRLECHR 250 260 270 280 290 300 QISGDWQTIN KLLTQVHDME TAFDGFWEKH QLKMEQYLQL WKFEQDFQQL VTEVEFLLNQ 310 320 330 340 350 360 QAELADVTGT IAQVKQKIKK LENLDENSQE LLSKAQFVIL HGHKLAANHH YALDLICQRC 370 380 390 400 410 420 NELRYLSDIL VNEIKAKRIQ LSRTFKMHKL LQQARQCCDE GECLLANQEI DKFQSKEDAQ 430 440 450 460 470 480 KALQDIENFL EMALPFINYE PETLQYEFDV ILSPELKVQM KTIQLKLENI RSIFENQQAG 490 500 510 520 530 540 FRNLADKHVR PIQFVVPTPE NLVTSGTPFF SSKQGKKTWR QNQSNLKIEV VPDCQEKRSS 550 560 570 580 590 600 GPSSSLDNGN SLDVLKNHVL NELIQTERVY VRELYTVLLG YRAEMDNPEM FDLMPPLLRN 610 620 630 640 650 660 KKDILFGNMA EIYEFHNDIF LSSLENCAHA PERVGPCFLE RKDDFQMYAK YCQNKPRSET 670 680 690 700 710 720 IWRKYSECAF FQECQRKLKH RLRLDSYLLK PVQRITKYQL LLKELLKYSK DCEGSALLKK 730 740 750 760 770 780 ALDAMLDLLK SVNDSMHQIA INGYIGNLNE LGKMIMQGGF SVWIGHKKGA TKMKDLARFK 790 800 810 820 830 840 PMQRHLFLYE KAIVFCKRRV ESGEGSDRYP SYSFKHCWKM DEVGITEYVK GDNRKFEIWY 850 860 870 880 890 900 GEKEEVYIVQ ASNVDVKMTW LKEIRNILLK QQELLTVKKR KQQDQLTERD KFQISLQQND 910 920 930 940 950 960 EKQQGAFIST EETELEHTST VVEVCEAIAS VQAEANTVWT EASQSAEISE EPAEWSSNYF 970 980 YPTYDENEEE NRPLMRPVSE MALLY
	Isoform 4 (Var.4) [UniParc]. Checksum: C5B1B4F9F368AC16	FASTA	941	109,436
	10 20 30 40 50 60 MAEANPRRGK MRFRRNAASF PGNLHLVLVL RPTSFLQRTF TDIGFWFSQE DFMLKLPVVM 70 80 90 100 110 120 LSSVSDLLTY IDDKQLTPEL GGTLQYCHSE WIIFRNAIEN FALTVKEMAQ MLQSFGTELA 130 140 150 160 170 180 ETELPDDIPS IEEILAIRAE RYHLLKNDIT AVTKEGKILL TNLEVPDTEG AVSSRLECHR 190 200 210 220 230 240 QISGDWQTIN KLLTQVHDME TAFDGFWEKH QLKMEQYLQL WKFEQDFQQL VTEVEFLLNQ 250 260 270 280 290 300 QAELADVTGT IAQVKQKIKK LENLDENSQE LLSKAQFVIL HGHKLAANHH YALDLICQRC 310 320 330 340 350 360 NELRYLSDIL VNEIKAKRIQ LSRTFKMHKL LQQARQCCDE GECLLANQEI DKFQSKEDAQ 370 380 390 400 410 420 KALQDIENFL EMALPFINYE PETLQYEFDV ILSPELKVQM KTIQLKLENI RSIFENQQAG 430 440 450 460 470 480 FRNLADKHVR PIQFVVPTPE NLVTSGTPFF SSKQVGVGYS FFQACKLFSK GKKTWRQNQS 490 500 510 520 530 540 NLKIEVVPDC QEKRSSGPSS SLDNGNSLDV LKNHVLNELI QTERVYVREL YTVLLGYRAE 550 560 570 580 590 600 MDNPEMFDLM PPLLRNKKDI LFGNMAEIYE FHNDIFLSSL ENCAHAPERV GPCFLERKDD 610 620 630 640 650 660 FQMYAKYCQN KPRSETIWRK YSECAFFQEC QRKLKHRLRL DSYLLKPVQR ITKYQLLLKE 670 680 690 700 710 720 LLKYSKDCEG SALLKKALDA MLDLLKSVND SMHQIAINGY IGNLNELGKM IMQGGFSVWI 730 740 750 760 770 780 GHKKGATKMK DLARFKPMQR HLFLYEKAIV FCKRRVESGE GSDRYPSYSF KHCWKMDEVG 790 800 810 820 830 840 ITEYVKGDNR KFEIWYGEKE EVYIVQASNV DVKMTWLKEI RNILLKQQEL LTVKKRKQQD 850 860 870 880 890 900 QLTERDKFQI SLQQNDEKQQ GAFISTEETE LEHTSTVVEV CEAIASVQAE ANTVWTEASQ 910 920 930 940 SAEISEEPAE WSSNYFYPTY DENEEENRPL MRPVSEMALL Y

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of the human dbl proto-oncogene: evidence that its overexpression is sufficient to transform NIH/3T3 cells." Ron D., Tronick S.R., Aaronson S.A., Eva A. EMBO J. 7:2465-2473(1988) [PubMed: 3056717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[2]	Ron D. Submitted (JUN-1989) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Alternative splicing variants of the human DBL (MCF-2) proto-oncogene." Komai K., Okayama R., Kitagawa M., Yagi H., Chihara K., Shiozawa S. Biochem. Biophys. Res. Commun. 299:455-458(2002) [PubMed: 12445822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4), TISSUE SPECIFICITY, CHARACTERIZATION.
[4]	Rhodes S., Huckle E. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[5]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed: 15772651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The predicted DBL oncogene product defines a distinct class of transforming proteins." Eva A., Vecchio G., Rao C.D., Tronick S.R., Aaronson S.A. Proc. Natl. Acad. Sci. U.S.A. 85:2061-2065(1988) [PubMed: 3281159] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 498-925 (ISOFORM 1).
[8]	"Activation of a mcf.2 oncogene by deletion of amino-terminal coding sequences." Noguchi T., Galland F., Batoz M., Mattei M.-G., Birnbaum D. Oncogene 3:709-715(1988) [PubMed: 2577874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 398-925 (ISOFORM 1).
[9]	"A region of proto-dbl essential for its transforming activity shows sequence similarity to a yeast cell cycle gene, CDC24, and the human breakpoint cluster gene, bcr." Ron D., Zannini M., Lewis M., Wickner R.B., Hunt L.T., Graziani G., Tronick S.R., Aaronson S.A., Eva A. New Biol. 3:372-379(1991) [PubMed: 2065022] [Abstract] Cited for: DOMAIN DBL-HOMOLOGY, MUTAGENESIS.
[10]	"Cellular transformation and guanine nucleotide exchange activity are catalyzed by a common domain on the dbl oncogene product." Hart M.J., Eva A., Zangrilli D., Aaronson S.A., Evans T., Cerione R.A., Zheng Y. J. Biol. Chem. 269:62-65(1994) [PubMed: 8276860] [Abstract] Cited for: CHARACTERIZATION OF DBL DOMAIN.
[11]	"Activation of the guanine nucleotide exchange factor Dbl following ACK1-dependent tyrosine phosphorylation." Kato J., Kaziro Y., Satoh T. Biochem. Biophys. Res. Commun. 268:141-147(2000) [PubMed: 10652228] [Abstract] Cited for: PHOSPHORYLATION BY TNK2.
[12]	"Role of the Sec14-like domain of Dbl family exchange factors in the regulation of Rho family GTPases in different subcellular sites." Ueda S., Kataoka T., Satoh T. Cell. Signal. 16:899-906(2004) [PubMed: 15157669] [Abstract] Cited for: SUBCELLULAR LOCATION, DOMAIN CRAL-TRIO, INTERACTION WITH INOSITOL PHOSPHOLIPIDS.
[13]	"Ccpg1, a novel scaffold protein that regulates the activity of the Rho guanine nucleotide exchange factor Dbs." Kostenko E.V., Olabisi O.O., Sahay S., Rodriguez P.L., Whitehead I.P. Mol. Cell. Biol. 26:8964-8975(2006) [PubMed: 17000758] [Abstract] Cited for: POSSIBLE INTERACTION WITH CCPG1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X12556 mRNA. Translation: CAA31069.1. AB085901 mRNA. Translation: BAC41200.1. AB085902 mRNA. Translation: BAC41201.1. AL117234 mRNA. Translation: CAB55301.1. AL033403 Genomic DNA. Translation: CAA21955.1. AL033403 Genomic DNA. Translation: CAI42107.1. AL033403 Genomic DNA. Translation: CAI42108.1. CH471150 Genomic DNA. Translation: EAW88427.1. CH471150 Genomic DNA. Translation: EAW88430.1. J03639 mRNA. Translation: AAA52172.1. Different initiation. X13230 mRNA. Translation: CAA31617.1. Sequence problems.
IPI	IPI00339309. IPI00339310. IPI00339312. IPI00641162.
PIR	TVHUBD. A28051. TVHUDB. A30040. S10138.
RefSeq	NP_001093325.1. NM_001099855.1. NP_001165347.1. NM_001171876.1. NP_001165348.1. NM_001171877.1. NP_001165349.1. NM_001171878.1. NP_001165350.1. NM_001171879.1. NP_005360.3. NM_005369.4.
UniGene	Hs.387262.
3D structure databases
ProteinModelPortal	P10911.
SMR	P10911. Positions 216-322, 491-811.
ModBase	Search...
Protein-protein interaction databases
IntAct	P10911. 2 interactions.
STRING	P10911.
PTM databases
PhosphoSite	P10911.
Polymorphism databases
DMDM	92087039.
Proteomic databases
PRIDE	P10911.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000370576; ENSP00000359608; ENSG00000101977.
GeneID	4168.
KEGG	hsa:4168.
UCSC	uc004fau.2. human. uc004faw.2. human.
Organism-specific databases
CTD	4168.
GeneCards	GC0XM138663.
HGNC	HGNC:6940. MCF2.
HPA	CAB010423.
MIM	311030. gene.
neXtProt	NX_P10911.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG10403.
HOVERGEN	HBG062385.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	P10911.
Bgee	P10911.
CleanEx	HS_MCF2.
Genevestigator	P10911.
GermOnline	ENSG00000101977. Homo sapiens.
Family and domain databases
InterPro	IPR001251. CRAL-TRIO_dom. IPR000219. DH-domain. IPR001331. GDS_CDC24_CS. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR018159. Spectrin/alpha-actinin. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:1.20.900.10. RhoGEF. 1 hit.
Pfam	PF00621. RhoGEF. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00325. RhoGEF. 1 hit. SM00150. SPEC. 1 hit. [Graphical view]
SUPFAM	SSF48065. DH-domain. 1 hit.
PROSITE	PS50191. CRAL_TRIO. 1 hit. PS00741. DH_1. 1 hit. PS50010. DH_2. 1 hit. PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	16414.
SOURCE	Search...

Entry information

Entry name

MCF2_HUMAN

Accession

Primary (citable) accession number: P10911
Secondary accession number(s): P14919 , Q5JYJ2, Q5JYJ3, Q5JYJ4, Q8IUF3, Q8IUF4, Q9UJB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 130 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.