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Protein

sn-glycerol-3-phosphate import ATP-binding protein UgpC

Gene

ugpC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex UgpABCE involved in sn-glycerol-3-phosphate import. Responsible for energy coupling to the transport system (Probable). Can also transport glycerophosphoryl diesters.UniRule annotationCurated5 Publications

Catalytic activityi

ATP + H2O + glycerol-3-phosphate(Out) = ADP + phosphate + glycerol-3-phosphate(In).UniRule annotation

Enzyme regulationi

Activated by gluconate, inhibited by fumarate and internal phosphate. Internal phosphate may bind to UgpC and reduce its affinity for UgpA and UgpE.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi37 – 448ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

  • carbohydrate transport Source: UniProtKB-KW
  • glycerol-3-phosphate transport Source: EcoCyc
  • glycerophosphodiester transport Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Sugar transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:UGPC-MONOMER.
ECOL316407:JW3415-MONOMER.
MetaCyc:UGPC-MONOMER.

Protein family/group databases

TCDBi3.A.1.1.3. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
sn-glycerol-3-phosphate import ATP-binding protein UgpCUniRule annotation (EC:3.6.3.20UniRule annotation)
Gene namesi
Name:ugpCUniRule annotation
Ordered Locus Names:b3450, JW3415
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11048. ugpC.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356sn-glycerol-3-phosphate import ATP-binding protein UgpCPRO_0000093026Add
BLAST

Proteomic databases

EPDiP10907.
PaxDbiP10907.

Expressioni

Inductioni

Induced by phosphate starvation, via PhoB. Also induced by carbon starvation, via the cAMP receptor protein (CRP).3 Publications

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (UgpC), two transmembrane proteins (UgpA and UgpE) and a solute-binding protein (UgpB).UniRule annotation

Protein-protein interaction databases

BioGridi4262493. 190 interactions.
DIPiDIP-11079N.
IntActiP10907. 8 interactions.
STRINGi511145.b3450.

Structurei

3D structure databases

ProteinModelPortaliP10907.
SMRiP10907. Positions 1-352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 235232ABC transporterUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ABC transporter superfamily. sn-glycerol-3-phosphate importer (TC 3.A.1.1.3) family. [View classification]UniRule annotation
Contains 1 ABC transporter domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108IJ9. Bacteria.
COG3839. LUCA.
InParanoidiP10907.
KOiK05816.
OMAiMTLPLEV.
OrthoDBiEOG6T7N3V.
PhylomeDBiP10907.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR008995. Mo/tungstate-bd_C_term_dom.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR013611. Transp-assoc_OB_typ2.
IPR017922. UgpC.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF08402. TOBE_2. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50331. SSF50331. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51315. UGPC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10907-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGLKLQAVT KSWDGKTQVI KPLTLDVADG EFIVMVGPSG CGKSTLLRMV
60 70 80 90 100
AGLERVTEGD IWINDQRVTE MEPKDRGIAM VFQNYALYPH MSVEENMAWG
110 120 130 140 150
LKIRGMGKQQ IAERVKEAAR ILELDGLLKR RPRELSGGQR QRVAMGRAIV
160 170 180 190 200
RDPAVFLFDE PLSNLDAKLR VQMRLELQQL HRRLKTTSLY VTHDQVEAMT
210 220 230 240 250
LAQRVMVMNG GVAEQIGTPV EVYEKPASLF VASFIGSPAM NLLTGRVNNE
260 270 280 290 300
GTHFELDGGI ELPLNGGYRQ YAGRKMTLGI RPEHIALSSQ AEGGVPMVMD
310 320 330 340 350
TLEILGADNL AHGRWGEQKL VVRLAHQERP TAGSTLWLHL AENQLHLFDG

ETGQRV
Length:356
Mass (Da):39,524
Last modified:February 1, 1995 - v3
Checksum:i33D49B258D0C9AA8
GO

Sequence cautioni

The sequence AAB18425.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13141 Genomic DNA. Translation: CAA31534.1.
U00039 Genomic DNA. Translation: AAB18425.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76475.2.
AP009048 Genomic DNA. Translation: BAE77843.1.
PIRiS47669. QRECUC.
RefSeqiNP_417907.2. NC_000913.3.
WP_000907792.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76475; AAC76475; b3450.
BAE77843; BAE77843; BAE77843.
GeneIDi947953.
KEGGiecj:JW3415.
eco:b3450.
PATRICi32122340. VBIEscCol129921_3547.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13141 Genomic DNA. Translation: CAA31534.1.
U00039 Genomic DNA. Translation: AAB18425.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76475.2.
AP009048 Genomic DNA. Translation: BAE77843.1.
PIRiS47669. QRECUC.
RefSeqiNP_417907.2. NC_000913.3.
WP_000907792.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP10907.
SMRiP10907. Positions 1-352.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262493. 190 interactions.
DIPiDIP-11079N.
IntActiP10907. 8 interactions.
STRINGi511145.b3450.

Protein family/group databases

TCDBi3.A.1.1.3. the atp-binding cassette (abc) superfamily.

Proteomic databases

EPDiP10907.
PaxDbiP10907.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76475; AAC76475; b3450.
BAE77843; BAE77843; BAE77843.
GeneIDi947953.
KEGGiecj:JW3415.
eco:b3450.
PATRICi32122340. VBIEscCol129921_3547.

Organism-specific databases

EchoBASEiEB1041.
EcoGeneiEG11048. ugpC.

Phylogenomic databases

eggNOGiENOG4108IJ9. Bacteria.
COG3839. LUCA.
InParanoidiP10907.
KOiK05816.
OMAiMTLPLEV.
OrthoDBiEOG6T7N3V.
PhylomeDBiP10907.

Enzyme and pathway databases

BioCyciEcoCyc:UGPC-MONOMER.
ECOL316407:JW3415-MONOMER.
MetaCyc:UGPC-MONOMER.

Miscellaneous databases

PROiP10907.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR008995. Mo/tungstate-bd_C_term_dom.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR013611. Transp-assoc_OB_typ2.
IPR017922. UgpC.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF08402. TOBE_2. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50331. SSF50331. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51315. UGPC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the ugp genes of Escherichia coli K-12: homology to the maltose system."
    Overduin P., Boos W., Tommassen J.
    Mol. Microbiol. 2:767-775(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "A second transport system for sn-glycerol-3-phosphate in Escherichia coli."
    Argast M., Ludtke D., Silhavy T.J., Boos W.
    J. Bacteriol. 136:1070-1083(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN SN-GLYCEROL-3-PHOSPHATE TRANSPORT, ENZYME REGULATION.
    Strain: K12.
  6. "Characteristics of a binding protein-dependent transport system for sn-glycerol-3-phosphate in Escherichia coli that is part of the pho regulon."
    Schweizer H., Argast M., Boos W.
    J. Bacteriol. 150:1154-1163(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN SN-GLYCEROL-3-PHOSPHATE TRANSPORT, INDUCTION BY PHOB.
    Strain: K12.
  7. "Characteristics of a ugp-encoded and phoB-dependent glycerophosphoryl diester phosphodiesterase which is physically dependent on the ugp transport system of Escherichia coli."
    Brzoska P., Boos W.
    J. Bacteriol. 170:4125-4135(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN SN-GLYCEROL-3-PHOSPHATE AND GLYCEROPHOSPHORYL DIESTERS TRANSPORT, INDUCTION BY PHOB.
    Strain: K12.
  8. "Dual regulation of the ugp operon by phosphate and carbon starvation at two interspaced promoters."
    Kasahara M., Makino K., Amemura M., Nakata A., Shinagawa H.
    J. Bacteriol. 173:549-558(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY PHOB, CRP.
    Strain: K12.
  9. "Functional exchangeability of the ABC proteins of the periplasmic binding protein-dependent transport systems Ugp and Mal of Escherichia coli."
    Hekstra D., Tommassen J.
    J. Bacteriol. 175:6546-6552(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTIONAL EXCHANGEABILITY OF UGPC AND MALK.
    Strain: K12.
  10. "The pho regulon-dependent Ugp uptake system for glycerol-3-phosphate in Escherichia coli is trans inhibited by Pi."
    Brzoska P., Rimmele M., Brzostek K., Boos W.
    J. Bacteriol. 176:15-20(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN SN-GLYCEROL-3-PHOSPHATE, INHIBITION BY INTERNAL PHOSPHATE.
    Strain: K12.
  11. "Kinetic analysis by in vivo 31P nuclear magnetic resonance of internal Pi during the uptake of sn-glycerol-3-phosphate by the pho regulon-dependent Ugp system and the glp regulon-dependent GlpT system."
    Xavier K.B., Kossmann M., Santos H., Boos W.
    J. Bacteriol. 177:699-704(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INHIBITION BY INTERNAL PHOSPHATE.
    Strain: K12.
  12. "Binding protein-dependent ABC transport system for glycerol 3-phosphate of Escherichia coli."
    Boos W.
    Methods Enzymol. 292:40-51(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiUGPC_ECOLI
AccessioniPrimary (citable) accession number: P10907
Secondary accession number(s): P76696, Q2M7B3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 1, 1995
Last modified: March 16, 2016
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Functional exchangeability of MalK and UgpC. But UgpC does not complement the regulatory function of MalK.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.