P10905 (UGPA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
sn-glycerol-3-phosphate transport system permease protein UgpA

Gene names

Name:	ugpA
Ordered Locus Names:	b3452, JW3417

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the binding-protein-dependent transport system for sn-glycerol-3-phosphate; probably responsible for the translocation of the substrate across the membrane.
Subunit structure	The complex is composed of two ATP-binding proteins (UgpC), two transmembrane proteins (UgpA and UgpE) and a solute-binding protein (UgpB) Probable.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the binding-protein-dependent transport system permease family. UgpAE subfamily. Contains 1 ABC transmembrane type-1 domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycerol-2-phosphate transport Inferred from mutant phenotype PubMed 19429609. Source: EcoCyc glycerol-3-phosphate transport Inferred from mutant phenotype PubMed 6281238. Source: EcoCyc
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	sn-glycerol-3-phosphate transport system permease protein UgpA		PRO_0000060232
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 32	21	Helical; Potential
	Topological domain	33 – 80	48	Periplasmic Potential
	Transmembrane	81 – 101	21	Helical; Potential
	Topological domain	102 – 109	8	Cytoplasmic Potential
	Transmembrane	110 – 130	21	Helical; Potential
	Topological domain	131 – 156	26	Periplasmic Potential
	Transmembrane	157 – 177	21	Helical; Potential
	Topological domain	178 – 207	30	Cytoplasmic Potential
	Transmembrane	208 – 228	21	Helical; Potential
	Topological domain	229 – 262	34	Periplasmic Potential
	Transmembrane	263 – 283	21	Helical; Potential
	Topological domain	284 – 295	12	Cytoplasmic Potential
	Domain	76 – 284	209	ABC transmembrane type-1

Sequences

	Sequence		Length	Mass (Da)
	P10905 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: CC8765DE0FB647A9	FASTA	295	33,264
	10 20 30 40 50 60 MSSSRPVFRS RWLPYLLVAP QLIITVIFFI WPAGEALWYS LQSVDPFGFS SQFVGLDNFV 70 80 90 100 110 120 TLFHDSYYLD SFWTTIKFST FVTVSGLLVS LFFAALVEYI VRGSRFYQTL MLLPYAVAPA 130 140 150 160 170 180 VAAVLWIFLF NPGRGLITHF LAEFGYDWNH AQNSGQAMFL VVFASVWKQI SYNFLFFYAA 190 200 210 220 230 240 LQSIPRSLIE AAAIDGAGPI RRFFKIALPL IAPVSFFLLV VNLVYAFFDT FPVIDAATSG 250 260 270 280 290 GPVQATTTLI YKIYREGFTG LDLASSAAQS VVLMFLVIVL TVVQFRYVES KVRYQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the ugp genes of Escherichia coli K-12: homology to the maltose system." Overduin P., Boos W., Tommassen J. Mol. Microbiol. 2:767-775(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13141 Genomic DNA. Translation: CAA31532.1. U00039 Genomic DNA. Translation: AAB18427.1. U00096 Genomic DNA. Translation: AAC76477.1. AP009048 Genomic DNA. Translation: BAE77841.1.
PIR	MMECUA. S03781.
RefSeq	NP_417909.1. NC_000913.2. YP_491982.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P10905.
SMR	P10905. Positions 55-282.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11077N.
STRING	511145.b3452.
Protein family/group databases
TCDB	3.A.1.1.3. ATP-binding cassette (ABC) superfamily.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76477; AAC76477; b3452. BAE77841; BAE77841; BAE77841.
GeneID	12934427. 947957.
KEGG	ecj:Y75_p3726. eco:b3452.
PATRIC	32122344. VBIEscCol129921_3549.
Organism-specific databases
EchoBASE	EB1039.
EcoGene	EG11046. ugpA.
Phylogenomic databases
eggNOG	COG1175.
HOGENOM	HOG000220406.
KO	K05814.
OMA	TIKFSTL.
ProtClustDB	PRK10561.
Enzyme and pathway databases
BioCyc	EcoCyc:UGPA-MONOMER. ECOL316407:JW3417-MONOMER.
BRENDA	3.6.3.20. 2026.
Gene expression databases
Genevestigator	P10905.
Family and domain databases
InterPro	IPR000515. BPD_transp. [Graphical view]
Pfam	PF00528. BPD_transp_1. 1 hit. [Graphical view]
PROSITE	PS50928. ABC_TM1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UGPA_ECOLI

Accession

Primary (citable) accession number: P10905
Secondary accession number(s): Q2M7B5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program