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Protein

Nitrate/nitrite transporter NarK

Gene

narK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes nitrate uptake, nitrite uptake and nitrite export across the cytoplasmic membrane. Functions as a nitrate/nitrite exchanger, and protons are probably not co-transported with the substrate.5 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei89SubstrateCurated1
Binding sitei305SubstrateCurated1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Nitrate assimilation, Transport

Enzyme and pathway databases

BioCyciEcoCyc:NARK-MONOMER.
ECOL316407:JW1214-MONOMER.
MetaCyc:NARK-MONOMER.

Protein family/group databases

TCDBi2.A.1.8.1. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate/nitrite transporter NarK
Alternative name(s):
Nitrite extrusion protein 1
Nitrite facilitator 1
Gene namesi
Name:narK
Ordered Locus Names:b1223, JW1214
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10642. narK.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 37CytoplasmicSequence analysisAdd BLAST37
Transmembranei38 – 58HelicalSequence analysisAdd BLAST21
Topological domaini59 – 78PeriplasmicSequence analysisAdd BLAST20
Transmembranei79 – 99HelicalSequence analysisAdd BLAST21
Topological domaini100 – 103CytoplasmicSequence analysis4
Transmembranei104 – 124HelicalSequence analysisAdd BLAST21
Topological domaini125 – 131PeriplasmicSequence analysis7
Transmembranei132 – 152HelicalSequence analysisAdd BLAST21
Topological domaini153 – 177CytoplasmicSequence analysisAdd BLAST25
Transmembranei178 – 198HelicalSequence analysisAdd BLAST21
Topological domaini199 – 211PeriplasmicSequence analysisAdd BLAST13
Transmembranei212 – 232HelicalSequence analysisAdd BLAST21
Topological domaini233 – 256CytoplasmicSequence analysisAdd BLAST24
Transmembranei257 – 277HelicalSequence analysisAdd BLAST21
Topological domaini278 – 283PeriplasmicSequence analysis6
Transmembranei284 – 304HelicalSequence analysisAdd BLAST21
Topological domaini305 – 319CytoplasmicSequence analysisAdd BLAST15
Transmembranei320 – 340HelicalSequence analysisAdd BLAST21
Topological domaini341 – 344PeriplasmicSequence analysis4
Transmembranei345 – 365HelicalSequence analysisAdd BLAST21
Topological domaini366 – 404CytoplasmicSequence analysisAdd BLAST39
Transmembranei405 – 425HelicalSequence analysisAdd BLAST21
Topological domaini426 – 435PeriplasmicSequence analysis10
Transmembranei436 – 456HelicalSequence analysisAdd BLAST21
Topological domaini457 – 463CytoplasmicSequence analysis7

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000967281 – 463Nitrate/nitrite transporter NarKAdd BLAST463

Proteomic databases

PaxDbiP10903.
PRIDEiP10903.

Expressioni

Inductioni

Highly expressed during anaerobic growth in the presence of nitrate.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4261633. 13 interactors.
STRINGi511145.b1223.

Structurei

Secondary structure

1463
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi13 – 16Combined sources4
Helixi23 – 28Combined sources6
Helixi30 – 52Combined sources23
Helixi55 – 61Combined sources7
Turni63 – 66Combined sources4
Helixi71 – 79Combined sources9
Helixi81 – 99Combined sources19
Helixi101 – 110Combined sources10
Helixi113 – 122Combined sources10
Beta strandi125 – 127Combined sources3
Helixi130 – 140Combined sources11
Helixi141 – 145Combined sources5
Helixi146 – 156Combined sources11
Helixi160 – 175Combined sources16
Helixi177 – 188Combined sources12
Helixi195 – 197Combined sources3
Helixi214 – 217Combined sources4
Helixi219 – 233Combined sources15
Helixi249 – 252Combined sources4
Helixi254 – 284Combined sources31
Helixi290 – 292Combined sources3
Turni293 – 295Combined sources3
Helixi296 – 315Combined sources20
Helixi317 – 334Combined sources18
Helixi335 – 338Combined sources4
Beta strandi342 – 344Combined sources3
Helixi348 – 387Combined sources40
Helixi392 – 414Combined sources23
Helixi417 – 432Combined sources16
Beta strandi433 – 435Combined sources3
Helixi436 – 459Combined sources24
Helixi461 – 463Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JR9X-ray2.60A1-463[»]
4JREX-ray2.80A/D1-463[»]
4U4TX-ray2.40A1-463[»]
4U4VX-ray2.35A1-463[»]
4U4WX-ray2.40A/B1-463[»]
ProteinModelPortaliP10903.
SMRiP10903.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DQQ. Bacteria.
COG2223. LUCA.
HOGENOMiHOG000241722.
InParanoidiP10903.
KOiK02575.
OMAiFAFFGPF.
PhylomeDBiP10903.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR004737. NO3_transporter.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00886. 2A0108. 1 hit.

Sequencei

Sequence statusi: Complete.

P10903-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHSSAPERA TGAVITDWRP EDPAFWQQRG QRIASRNLWI SVPCLLLAFC
60 70 80 90 100
VWMLFSAVAV NLPKVGFNFT TDQLFMLTAL PSVSGALLRV PYSFMVPIFG
110 120 130 140 150
GRRWTAFSTG ILIIPCVWLG FAVQDTSTPY SVFIIISLLC GFAGANFASS
160 170 180 190 200
MANISFFFPK QKQGGALGLN GGLGNMGVSV MQLVAPLVVS LSIFAVFGSQ
210 220 230 240 250
GVKQPDGTEL YLANASWIWV PFLAIFTIAA WFGMNDLATS KASIKEQLPV
260 270 280 290 300
LKRGHLWIMS LLYLATFGSF IGFSAGFAML SKTQFPDVQI LQYAFFGPFI
310 320 330 340 350
GALARSAGGA LSDRLGGTRV TLVNFILMAI FSGLLFLTLP TDGQGGSFMA
360 370 380 390 400
FFAVFLALFL TAGLGSGSTF QMISVIFRKL TMDRVKAEGG SDERAMREAA
410 420 430 440 450
TDTAAALGFI SAIGAIGGFF IPKAFGSSLA LTGSPVGAMK VFLIFYIACV
460
VITWAVYGRH SKK
Length:463
Mass (Da):49,693
Last modified:January 1, 1990 - v2
Checksum:iAF1DD67CAD40FE8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15996 Genomic DNA. Translation: CAA34126.1.
X69189 Genomic DNA. Translation: CAA48933.1.
U00096 Genomic DNA. Translation: AAC74307.1.
AP009048 Genomic DNA. Translation: BAA36091.1.
X13360 Genomic DNA. Translation: CAA31740.1.
PIRiS05239. GRECNK.
RefSeqiNP_415741.1. NC_000913.3.
WP_000019827.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74307; AAC74307; b1223.
BAA36091; BAA36091; BAA36091.
GeneIDi945783.
KEGGiecj:JW1214.
eco:b1223.
PATRICi32117706. VBIEscCol129921_1275.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15996 Genomic DNA. Translation: CAA34126.1.
X69189 Genomic DNA. Translation: CAA48933.1.
U00096 Genomic DNA. Translation: AAC74307.1.
AP009048 Genomic DNA. Translation: BAA36091.1.
X13360 Genomic DNA. Translation: CAA31740.1.
PIRiS05239. GRECNK.
RefSeqiNP_415741.1. NC_000913.3.
WP_000019827.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JR9X-ray2.60A1-463[»]
4JREX-ray2.80A/D1-463[»]
4U4TX-ray2.40A1-463[»]
4U4VX-ray2.35A1-463[»]
4U4WX-ray2.40A/B1-463[»]
ProteinModelPortaliP10903.
SMRiP10903.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261633. 13 interactors.
STRINGi511145.b1223.

Protein family/group databases

TCDBi2.A.1.8.1. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbiP10903.
PRIDEiP10903.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74307; AAC74307; b1223.
BAA36091; BAA36091; BAA36091.
GeneIDi945783.
KEGGiecj:JW1214.
eco:b1223.
PATRICi32117706. VBIEscCol129921_1275.

Organism-specific databases

EchoBASEiEB0636.
EcoGeneiEG10642. narK.

Phylogenomic databases

eggNOGiENOG4105DQQ. Bacteria.
COG2223. LUCA.
HOGENOMiHOG000241722.
InParanoidiP10903.
KOiK02575.
OMAiFAFFGPF.
PhylomeDBiP10903.

Enzyme and pathway databases

BioCyciEcoCyc:NARK-MONOMER.
ECOL316407:JW1214-MONOMER.
MetaCyc:NARK-MONOMER.

Miscellaneous databases

PROiP10903.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR004737. NO3_transporter.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00886. 2A0108. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNARK_ECOLI
AccessioniPrimary (citable) accession number: P10903
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 1, 1990
Last modified: November 2, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.