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Protein

Nitrate/nitrite transporter NarK

Gene

narK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes nitrate uptake, nitrite uptake and nitrite export across the cytoplasmic membrane. Functions as a nitrate/nitrite exchanger, and protons are probably not co-transported with the substrate.5 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei89SubstrateCurated1
Binding sitei305SubstrateCurated1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processNitrate assimilation, Transport

Enzyme and pathway databases

BioCyciEcoCyc:NARK-MONOMER
MetaCyc:NARK-MONOMER

Protein family/group databases

TCDBi2.A.1.8.1 the major facilitator superfamily (mfs)

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate/nitrite transporter NarK
Alternative name(s):
Nitrite extrusion protein 1
Nitrite facilitator 1
Gene namesi
Name:narK
Ordered Locus Names:b1223, JW1214
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10642 narK

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 37CytoplasmicSequence analysisAdd BLAST37
Transmembranei38 – 58HelicalSequence analysisAdd BLAST21
Topological domaini59 – 78PeriplasmicSequence analysisAdd BLAST20
Transmembranei79 – 99HelicalSequence analysisAdd BLAST21
Topological domaini100 – 103CytoplasmicSequence analysis4
Transmembranei104 – 124HelicalSequence analysisAdd BLAST21
Topological domaini125 – 131PeriplasmicSequence analysis7
Transmembranei132 – 152HelicalSequence analysisAdd BLAST21
Topological domaini153 – 177CytoplasmicSequence analysisAdd BLAST25
Transmembranei178 – 198HelicalSequence analysisAdd BLAST21
Topological domaini199 – 211PeriplasmicSequence analysisAdd BLAST13
Transmembranei212 – 232HelicalSequence analysisAdd BLAST21
Topological domaini233 – 256CytoplasmicSequence analysisAdd BLAST24
Transmembranei257 – 277HelicalSequence analysisAdd BLAST21
Topological domaini278 – 283PeriplasmicSequence analysis6
Transmembranei284 – 304HelicalSequence analysisAdd BLAST21
Topological domaini305 – 319CytoplasmicSequence analysisAdd BLAST15
Transmembranei320 – 340HelicalSequence analysisAdd BLAST21
Topological domaini341 – 344PeriplasmicSequence analysis4
Transmembranei345 – 365HelicalSequence analysisAdd BLAST21
Topological domaini366 – 404CytoplasmicSequence analysisAdd BLAST39
Transmembranei405 – 425HelicalSequence analysisAdd BLAST21
Topological domaini426 – 435PeriplasmicSequence analysis10
Transmembranei436 – 456HelicalSequence analysisAdd BLAST21
Topological domaini457 – 463CytoplasmicSequence analysis7

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000967281 – 463Nitrate/nitrite transporter NarKAdd BLAST463

Proteomic databases

PaxDbiP10903
PRIDEiP10903

Expressioni

Inductioni

Highly expressed during anaerobic growth in the presence of nitrate.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4261633, 21 interactors
STRINGi316385.ECDH10B_1282

Structurei

Secondary structure

1463
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi13 – 16Combined sources4
Helixi23 – 28Combined sources6
Helixi30 – 52Combined sources23
Helixi55 – 61Combined sources7
Turni63 – 66Combined sources4
Helixi71 – 79Combined sources9
Helixi81 – 99Combined sources19
Helixi101 – 110Combined sources10
Helixi113 – 122Combined sources10
Beta strandi125 – 127Combined sources3
Helixi130 – 140Combined sources11
Helixi141 – 145Combined sources5
Helixi146 – 156Combined sources11
Helixi160 – 175Combined sources16
Helixi177 – 188Combined sources12
Helixi195 – 197Combined sources3
Helixi214 – 217Combined sources4
Helixi219 – 233Combined sources15
Helixi249 – 252Combined sources4
Helixi254 – 284Combined sources31
Helixi290 – 292Combined sources3
Turni293 – 295Combined sources3
Helixi296 – 315Combined sources20
Helixi317 – 334Combined sources18
Helixi335 – 338Combined sources4
Beta strandi342 – 344Combined sources3
Helixi348 – 387Combined sources40
Helixi392 – 414Combined sources23
Helixi417 – 432Combined sources16
Beta strandi433 – 435Combined sources3
Helixi436 – 459Combined sources24
Helixi461 – 463Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JR9X-ray2.60A1-463[»]
4JREX-ray2.80A/D1-463[»]
4U4TX-ray2.40A1-463[»]
4U4VX-ray2.35A1-463[»]
4U4WX-ray2.40A/B1-463[»]
ProteinModelPortaliP10903
SMRiP10903
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DQQ Bacteria
COG2223 LUCA
HOGENOMiHOG000241722
InParanoidiP10903
KOiK02575
OMAiNVGAYGN
PhylomeDBiP10903

Family and domain databases

InterProiView protein in InterPro
IPR011701 MFS
IPR036259 MFS_trans_sf
IPR004737 NO3_transporter
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 1 hit
TIGRFAMsiTIGR00886 2A0108, 1 hit

Sequencei

Sequence statusi: Complete.

P10903-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHSSAPERA TGAVITDWRP EDPAFWQQRG QRIASRNLWI SVPCLLLAFC
60 70 80 90 100
VWMLFSAVAV NLPKVGFNFT TDQLFMLTAL PSVSGALLRV PYSFMVPIFG
110 120 130 140 150
GRRWTAFSTG ILIIPCVWLG FAVQDTSTPY SVFIIISLLC GFAGANFASS
160 170 180 190 200
MANISFFFPK QKQGGALGLN GGLGNMGVSV MQLVAPLVVS LSIFAVFGSQ
210 220 230 240 250
GVKQPDGTEL YLANASWIWV PFLAIFTIAA WFGMNDLATS KASIKEQLPV
260 270 280 290 300
LKRGHLWIMS LLYLATFGSF IGFSAGFAML SKTQFPDVQI LQYAFFGPFI
310 320 330 340 350
GALARSAGGA LSDRLGGTRV TLVNFILMAI FSGLLFLTLP TDGQGGSFMA
360 370 380 390 400
FFAVFLALFL TAGLGSGSTF QMISVIFRKL TMDRVKAEGG SDERAMREAA
410 420 430 440 450
TDTAAALGFI SAIGAIGGFF IPKAFGSSLA LTGSPVGAMK VFLIFYIACV
460
VITWAVYGRH SKK
Length:463
Mass (Da):49,693
Last modified:January 1, 1990 - v2
Checksum:iAF1DD67CAD40FE8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15996 Genomic DNA Translation: CAA34126.1
X69189 Genomic DNA Translation: CAA48933.1
U00096 Genomic DNA Translation: AAC74307.1
AP009048 Genomic DNA Translation: BAA36091.1
X13360 Genomic DNA Translation: CAA31740.1
PIRiS05239 GRECNK
RefSeqiNP_415741.1, NC_000913.3
WP_000019827.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74307; AAC74307; b1223
BAA36091; BAA36091; BAA36091
GeneIDi945783
KEGGiecj:JW1214
eco:b1223
PATRICifig|1411691.4.peg.1058

Similar proteinsi

Entry informationi

Entry nameiNARK_ECOLI
AccessioniPrimary (citable) accession number: P10903
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 1, 1990
Last modified: March 28, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health