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P10895 (PLCD1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 144. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1

EC=3.1.4.11
Alternative name(s):
Phosphoinositide phospholipase C-delta-1
Phospholipase C-III
Short name=PLC-III
Phospholipase C-delta-1
Short name=PLC-delta-1
Gene names
Name:PLCD1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length756 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development.

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactor

Binds 3 calcium ions per subunit. Two of the calcium ions are bound to the C2 domain By similarity.

Sequence similarities

Contains 1 C2 domain.

Contains 2 EF-hand domains.

Contains 1 PH domain.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7567561-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1
PRO_0000088503

Regions

Domain21 – 130110PH
Domain140 – 17536EF-hand 1
Domain176 – 21136EF-hand 2
Domain296 – 440145PI-PLC X-box
Domain492 – 609118PI-PLC Y-box
Domain628 – 72093C2
Calcium binding153 – 164121 Potential
Calcium binding189 – 200122 Potential

Sites

Active site3111 By similarity
Active site3561 By similarity
Metal binding3121Calcium 1; catalytic By similarity
Metal binding3411Calcium 1; catalytic By similarity
Metal binding3431Calcium 1; catalytic By similarity
Metal binding3901Calcium 1; catalytic By similarity
Metal binding6511Calcium 2; via carbonyl oxygen By similarity
Metal binding6531Calcium 2 By similarity
Metal binding6771Calcium 2 By similarity
Metal binding7061Calcium 3 By similarity
Metal binding7071Calcium 3; via carbonyl oxygen By similarity
Metal binding7081Calcium 3 By similarity
Binding site4381Substrate By similarity
Binding site4401Substrate By similarity
Binding site5221Substrate By similarity
Binding site5491Substrate By similarity

Experimental info

Sequence conflict2911H → D in AAA30710. Ref.2
Sequence conflict664 – 6652SR → TG in AAA30710. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P10895 [UniParc].

Last modified November 13, 2007. Version 2.
Checksum: EBA47E8BAEE2D398

FASTA75686,073
        10         20         30         40         50         60 
MDSGRDFLTL HGLQDDKDLQ ALLKGGQLLK VKSNSWRRER FYKLQEDCKT IWQESRKVMR 

        70         80         90        100        110        120 
TPESQLFSIE DIQEVRMGHR TEGLEKFARD VPENRCFSIV FKDQRNTLDL IAPSPADAQH 

       130        140        150        160        170        180 
WVQGLGKIIH HSGSMDQQQK LRHWIHSCLR KADKNKDNKM SFKELQNFLK ELNIQVDDSY 

       190        200        210        220        230        240 
ARKIFKECDH SQTDSLEDEE IETFYKILTQ RKEIDRTFEE ATGSKETLSV DQLVTFLQHQ 

       250        260        270        280        290        300 
QREEEAGPAL ALSLIERYEP SETAKAQRQM TKDGFLMYLL SADGSAFDLA HRRVYQDMDQ 

       310        320        330        340        350        360 
PLSHYLVSSS HNTYLLEDQL TGPSSTEAYI RALCKGCRCL ELDCWDGPNQ EPIIYHGYTF 

       370        380        390        400        410        420 
TSKILFCDVV RAIRDYAFKA SPYPVILSLE NHCSLEQQRV MARHLRTLLG PMLLDRPLDG 

       430        440        450        460        470        480 
VVTSLPSPEQ LRGKILLKGK KLGGLFPPGG EGGPEATVVS DEDEAAEMED EAVRSQVQHK 

       490        500        510        520        530        540 
SKEDKLRLAK ELSDMVIYCK SVHFRGFPSS GTSGQAFYEM SSFSENRALR LLQESGNSFV 

       550        560        570        580        590        600 
RHNVNHLSRI YPAGWRTDSS NYSPVEMWNG GCQIVALNFQ TPGSEMDVYQ GRFLDNGACG 

       610        620        630        640        650        660 
YVLKPAFLRD PNSTFNSRAL AHGPWWTPKR LNVRVISGQQ LPKVNKNKNS IVDPKVTVEI 

       670        680        690        700        710        720 
HGVSRDVASR QTAVVTNNGF NPWWDTELEF EVAVPELALV RFVVEDYDAS SKNDFIGQST 

       730        740        750 
IPLKSLKQGY RHIHLLSKNG DQHPSATLFV KVALQD 

« Hide

References

« Hide 'large scale' references
[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Fetal skin.
[2]"Cloning and sequence of multiple forms of phospholipase C."
Suh P.-G., Ryu S.H., Moon K.H., Suh H.W., Rhee S.G.
Cell 54:161-169(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 62-756.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC133304 mRNA. Translation: AAI33305.1.
M20638 mRNA. Translation: AAA30710.1.
PIRC28821.
RefSeqNP_001075045.1. NM_001081576.1.
XP_001256610.2. XM_001256609.4.
UniGeneBt.4877.

3D structure databases

ProteinModelPortalP10895.
SMRP10895. Positions 12-130, 158-756.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP10895.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000052073; ENSBTAP00000049395; ENSBTAG00000037726.
GeneID281986.
790012.
KEGGbta:281986.
bta:790012.

Organism-specific databases

CTD5333.

Phylogenomic databases

eggNOGNOG149692.
GeneTreeENSGT00740000114979.
HOGENOMHOG000006871.
HOVERGENHBG053610.
InParanoidP10895.
KOK05857.
OMATSGQAFY.
OrthoDBEOG7V49XT.
TreeFamTF313216.

Family and domain databases

Gene3D1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR028391. PLC-delta1.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR001849. Pleckstrin_homology.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF80. PTHR10336:SF80. 1 hit.
PfamPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00169. PH. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSPR00390. PHPHLIPASEC.
SMARTSM00239. C2. 1 hit.
SM00233. PH. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805854.

Entry information

Entry namePLCD1_BOVIN
AccessionPrimary (citable) accession number: P10895
Secondary accession number(s): A2VDM2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 13, 2007
Last modified: June 11, 2014
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families