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P10895

- PLCD1_BOVIN

UniProt

P10895 - PLCD1_BOVIN

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1

Gene

PLCD1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 2 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development.

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Binds 3 calcium ions per subunit. Two of the calcium ions are bound to the C2 domain By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei311 – 3111PROSITE-ProRule annotation
    Metal bindingi312 – 3121Calcium 1; catalyticBy similarity
    Metal bindingi341 – 3411Calcium 1; catalyticBy similarity
    Metal bindingi343 – 3431Calcium 1; catalyticBy similarity
    Active sitei356 – 3561PROSITE-ProRule annotation
    Metal bindingi390 – 3901Calcium 1; catalyticBy similarity
    Binding sitei438 – 4381SubstrateBy similarity
    Binding sitei440 – 4401SubstrateBy similarity
    Binding sitei522 – 5221SubstrateBy similarity
    Binding sitei549 – 5491SubstrateBy similarity
    Metal bindingi651 – 6511Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi653 – 6531Calcium 2By similarity
    Metal bindingi677 – 6771Calcium 2By similarity
    Metal bindingi706 – 7061Calcium 3By similarity
    Metal bindingi707 – 7071Calcium 3; via carbonyl oxygenBy similarity
    Metal bindingi708 – 7081Calcium 3By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi153 – 164121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi189 – 200122PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. angiogenesis Source: Ensembl
    2. intracellular signal transduction Source: InterPro
    3. labyrinthine layer blood vessel development Source: Ensembl
    4. lipid catabolic process Source: UniProtKB-KW
    5. regulation of cell proliferation Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_225102. Synthesis of IP3 and IP4 in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase C-delta-1
    Phospholipase C-III
    Short name:
    PLC-III
    Phospholipase C-delta-1
    Short name:
    PLC-delta-1
    Gene namesi
    Name:PLCD1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 22

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7567561-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1PRO_0000088503Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi191 – 1911O-linked (GlcNAc)By similarity
    Glycosylationi193 – 1931O-linked (GlcNAc)By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiP10895.

    Structurei

    3D structure databases

    ProteinModelPortaliP10895.
    SMRiP10895. Positions 12-130, 158-756.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 130110PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini140 – 17536EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini176 – 21136EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini296 – 440145PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini492 – 609118PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini628 – 72093C2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 2 EF-hand domains.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG149692.
    GeneTreeiENSGT00740000114979.
    HOGENOMiHOG000006871.
    HOVERGENiHBG053610.
    InParanoidiP10895.
    KOiK05857.
    OMAiTSGQAFY.
    OrthoDBiEOG7V49XT.
    TreeFamiTF313216.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR028391. PLC-delta1.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF80. PTHR10336:SF80. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00169. PH. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00233. PH. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS50003. PH_DOMAIN. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P10895-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSGRDFLTL HGLQDDKDLQ ALLKGGQLLK VKSNSWRRER FYKLQEDCKT    50
    IWQESRKVMR TPESQLFSIE DIQEVRMGHR TEGLEKFARD VPENRCFSIV 100
    FKDQRNTLDL IAPSPADAQH WVQGLGKIIH HSGSMDQQQK LRHWIHSCLR 150
    KADKNKDNKM SFKELQNFLK ELNIQVDDSY ARKIFKECDH SQTDSLEDEE 200
    IETFYKILTQ RKEIDRTFEE ATGSKETLSV DQLVTFLQHQ QREEEAGPAL 250
    ALSLIERYEP SETAKAQRQM TKDGFLMYLL SADGSAFDLA HRRVYQDMDQ 300
    PLSHYLVSSS HNTYLLEDQL TGPSSTEAYI RALCKGCRCL ELDCWDGPNQ 350
    EPIIYHGYTF TSKILFCDVV RAIRDYAFKA SPYPVILSLE NHCSLEQQRV 400
    MARHLRTLLG PMLLDRPLDG VVTSLPSPEQ LRGKILLKGK KLGGLFPPGG 450
    EGGPEATVVS DEDEAAEMED EAVRSQVQHK SKEDKLRLAK ELSDMVIYCK 500
    SVHFRGFPSS GTSGQAFYEM SSFSENRALR LLQESGNSFV RHNVNHLSRI 550
    YPAGWRTDSS NYSPVEMWNG GCQIVALNFQ TPGSEMDVYQ GRFLDNGACG 600
    YVLKPAFLRD PNSTFNSRAL AHGPWWTPKR LNVRVISGQQ LPKVNKNKNS 650
    IVDPKVTVEI HGVSRDVASR QTAVVTNNGF NPWWDTELEF EVAVPELALV 700
    RFVVEDYDAS SKNDFIGQST IPLKSLKQGY RHIHLLSKNG DQHPSATLFV 750
    KVALQD 756
    Length:756
    Mass (Da):86,073
    Last modified:November 13, 2007 - v2
    Checksum:iEBA47E8BAEE2D398
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti291 – 2911H → D in AAA30710. (PubMed:3390863)Curated
    Sequence conflicti664 – 6652SR → TG in AAA30710. (PubMed:3390863)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC133304 mRNA. Translation: AAI33305.1.
    M20638 mRNA. Translation: AAA30710.1.
    PIRiC28821.
    RefSeqiNP_001075045.1. NM_001081576.1.
    XP_001256610.2. XM_001256609.4.
    UniGeneiBt.4877.

    Genome annotation databases

    EnsembliENSBTAT00000052073; ENSBTAP00000049395; ENSBTAG00000037726.
    GeneIDi281986.
    790012.
    KEGGibta:281986.
    bta:790012.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC133304 mRNA. Translation: AAI33305.1 .
    M20638 mRNA. Translation: AAA30710.1 .
    PIRi C28821.
    RefSeqi NP_001075045.1. NM_001081576.1.
    XP_001256610.2. XM_001256609.4.
    UniGenei Bt.4877.

    3D structure databases

    ProteinModelPortali P10895.
    SMRi P10895. Positions 12-130, 158-756.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P10895.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000052073 ; ENSBTAP00000049395 ; ENSBTAG00000037726 .
    GeneIDi 281986.
    790012.
    KEGGi bta:281986.
    bta:790012.

    Organism-specific databases

    CTDi 5333.

    Phylogenomic databases

    eggNOGi NOG149692.
    GeneTreei ENSGT00740000114979.
    HOGENOMi HOG000006871.
    HOVERGENi HBG053610.
    InParanoidi P10895.
    KOi K05857.
    OMAi TSGQAFY.
    OrthoDBi EOG7V49XT.
    TreeFami TF313216.

    Enzyme and pathway databases

    Reactomei REACT_225102. Synthesis of IP3 and IP4 in the cytosol.

    Miscellaneous databases

    NextBioi 20805854.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR028391. PLC-delta1.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF80. PTHR10336:SF80. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00169. PH. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00233. PH. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS50003. PH_DOMAIN. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal skin.
    2. "Cloning and sequence of multiple forms of phospholipase C."
      Suh P.-G., Ryu S.H., Moon K.H., Suh H.W., Rhee S.G.
      Cell 54:161-169(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 62-756.

    Entry informationi

    Entry nameiPLCD1_BOVIN
    AccessioniPrimary (citable) accession number: P10895
    Secondary accession number(s): A2VDM2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 146 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3