Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1

Gene

PLCD1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Ca2+By similarityNote: Binds 3 Ca(2+) ions per subunit. Two of the Ca2+ ions are bound to the C2 domain.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei311 – 3111PROSITE-ProRule annotation
Metal bindingi312 – 3121Calcium 1; catalyticBy similarity
Metal bindingi341 – 3411Calcium 1; catalyticBy similarity
Metal bindingi343 – 3431Calcium 1; catalyticBy similarity
Active sitei356 – 3561PROSITE-ProRule annotation
Metal bindingi390 – 3901Calcium 1; catalyticBy similarity
Binding sitei438 – 4381SubstrateBy similarity
Binding sitei440 – 4401SubstrateBy similarity
Binding sitei522 – 5221SubstrateBy similarity
Binding sitei549 – 5491SubstrateBy similarity
Metal bindingi651 – 6511Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi653 – 6531Calcium 2By similarity
Metal bindingi677 – 6771Calcium 2By similarity
Metal bindingi706 – 7061Calcium 3By similarity
Metal bindingi707 – 7071Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi708 – 7081Calcium 3By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi153 – 164121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi189 – 200122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidic acid binding Source: Ensembl
  3. phosphatidylinositol phosphate binding Source: Ensembl
  4. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  5. phosphatidylserine binding Source: Ensembl
  6. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. angiogenesis Source: Ensembl
  2. intracellular signal transduction Source: InterPro
  3. labyrinthine layer blood vessel development Source: Ensembl
  4. lipid catabolic process Source: UniProtKB-KW
  5. regulation of cell proliferation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_340083. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-delta-1
Phospholipase C-III
Short name:
PLC-III
Phospholipase C-delta-1
Short name:
PLC-delta-1
Gene namesi
Name:PLCD1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 22

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7567561-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1PRO_0000088503Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi191 – 1911O-linked (GlcNAc)By similarity
Glycosylationi193 – 1931O-linked (GlcNAc)By similarity
Modified residuei460 – 4601PhosphoserineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP10895.

Structurei

3D structure databases

ProteinModelPortaliP10895.
SMRiP10895. Positions 12-130, 158-756.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 130110PHPROSITE-ProRule annotationAdd
BLAST
Domaini140 – 17536EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini176 – 21136EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini296 – 440145PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini492 – 609118PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini628 – 72093C2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00760000118936.
HOGENOMiHOG000006871.
HOVERGENiHBG053610.
InParanoidiP10895.
KOiK05857.
OMAiAVRSQVQ.
OrthoDBiEOG7V49XT.
TreeFamiTF313216.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR028391. PLC-delta1.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF80. PTHR10336:SF80. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00169. PH. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00233. PH. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10895-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSGRDFLTL HGLQDDKDLQ ALLKGGQLLK VKSNSWRRER FYKLQEDCKT
60 70 80 90 100
IWQESRKVMR TPESQLFSIE DIQEVRMGHR TEGLEKFARD VPENRCFSIV
110 120 130 140 150
FKDQRNTLDL IAPSPADAQH WVQGLGKIIH HSGSMDQQQK LRHWIHSCLR
160 170 180 190 200
KADKNKDNKM SFKELQNFLK ELNIQVDDSY ARKIFKECDH SQTDSLEDEE
210 220 230 240 250
IETFYKILTQ RKEIDRTFEE ATGSKETLSV DQLVTFLQHQ QREEEAGPAL
260 270 280 290 300
ALSLIERYEP SETAKAQRQM TKDGFLMYLL SADGSAFDLA HRRVYQDMDQ
310 320 330 340 350
PLSHYLVSSS HNTYLLEDQL TGPSSTEAYI RALCKGCRCL ELDCWDGPNQ
360 370 380 390 400
EPIIYHGYTF TSKILFCDVV RAIRDYAFKA SPYPVILSLE NHCSLEQQRV
410 420 430 440 450
MARHLRTLLG PMLLDRPLDG VVTSLPSPEQ LRGKILLKGK KLGGLFPPGG
460 470 480 490 500
EGGPEATVVS DEDEAAEMED EAVRSQVQHK SKEDKLRLAK ELSDMVIYCK
510 520 530 540 550
SVHFRGFPSS GTSGQAFYEM SSFSENRALR LLQESGNSFV RHNVNHLSRI
560 570 580 590 600
YPAGWRTDSS NYSPVEMWNG GCQIVALNFQ TPGSEMDVYQ GRFLDNGACG
610 620 630 640 650
YVLKPAFLRD PNSTFNSRAL AHGPWWTPKR LNVRVISGQQ LPKVNKNKNS
660 670 680 690 700
IVDPKVTVEI HGVSRDVASR QTAVVTNNGF NPWWDTELEF EVAVPELALV
710 720 730 740 750
RFVVEDYDAS SKNDFIGQST IPLKSLKQGY RHIHLLSKNG DQHPSATLFV

KVALQD
Length:756
Mass (Da):86,073
Last modified:November 12, 2007 - v2
Checksum:iEBA47E8BAEE2D398
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti291 – 2911H → D in AAA30710 (PubMed:3390863).Curated
Sequence conflicti664 – 6652SR → TG in AAA30710 (PubMed:3390863).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC133304 mRNA. Translation: AAI33305.1.
M20638 mRNA. Translation: AAA30710.1.
PIRiC28821.
RefSeqiNP_001075045.1. NM_001081576.1.
XP_001256610.2. XM_001256609.5.
UniGeneiBt.4877.

Genome annotation databases

EnsembliENSBTAT00000052073; ENSBTAP00000049395; ENSBTAG00000037726.
GeneIDi281986.
790012.
KEGGibta:281986.
bta:790012.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC133304 mRNA. Translation: AAI33305.1.
M20638 mRNA. Translation: AAA30710.1.
PIRiC28821.
RefSeqiNP_001075045.1. NM_001081576.1.
XP_001256610.2. XM_001256609.5.
UniGeneiBt.4877.

3D structure databases

ProteinModelPortaliP10895.
SMRiP10895. Positions 12-130, 158-756.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP10895.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000052073; ENSBTAP00000049395; ENSBTAG00000037726.
GeneIDi281986.
790012.
KEGGibta:281986.
bta:790012.

Organism-specific databases

CTDi5333.

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00760000118936.
HOGENOMiHOG000006871.
HOVERGENiHBG053610.
InParanoidiP10895.
KOiK05857.
OMAiAVRSQVQ.
OrthoDBiEOG7V49XT.
TreeFamiTF313216.

Enzyme and pathway databases

ReactomeiREACT_340083. Synthesis of IP3 and IP4 in the cytosol.

Miscellaneous databases

NextBioi20805854.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR028391. PLC-delta1.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF80. PTHR10336:SF80. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00169. PH. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00233. PH. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal skin.
  2. "Cloning and sequence of multiple forms of phospholipase C."
    Suh P.-G., Ryu S.H., Moon K.H., Suh H.W., Rhee S.G.
    Cell 54:161-169(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 62-756.

Entry informationi

Entry nameiPLCD1_BOVIN
AccessioniPrimary (citable) accession number: P10895
Secondary accession number(s): A2VDM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 30, 1989
Last sequence update: November 12, 2007
Last modified: March 31, 2015
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.