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P10894

- PLCB1_BOVIN

UniProt

P10894 - PLCB1_BOVIN

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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1

Gene
PLCB1
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Calcium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei331 – 3311 By similarity
Active sitei378 – 3781 By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. GTPase activator activity Source: Ensembl
  3. phosphatidylinositol-4,5-bisphosphate binding Source: Ensembl
  4. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  5. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. activation of meiosis involved in egg activation Source: Ensembl
  2. cerebral cortex development Source: Ensembl
  3. fat cell differentiation Source: Ensembl
  4. glutamate receptor signaling pathway Source: Ensembl
  5. G-protein coupled acetylcholine receptor signaling pathway Source: Ensembl
  6. insulin-like growth factor receptor signaling pathway Source: Ensembl
  7. interleukin-12-mediated signaling pathway Source: Ensembl
  8. interleukin-15-mediated signaling pathway Source: Ensembl
  9. interleukin-1-mediated signaling pathway Source: Ensembl
  10. intracellular signal transduction Source: InterPro
  11. lipid catabolic process Source: UniProtKB-KW
  12. memory Source: Ensembl
  13. negative regulation of monocyte extravasation Source: Ensembl
  14. negative regulation of transcription, DNA-templated Source: Ensembl
  15. positive regulation of acrosome reaction Source: Ensembl
  16. positive regulation of CD24 biosynthetic process Source: Ensembl
  17. positive regulation of developmental growth Source: Ensembl
  18. positive regulation of embryonic development Source: Ensembl
  19. positive regulation of G1/S transition of mitotic cell cycle Source: Ensembl
  20. positive regulation of interleukin-12 production Source: Ensembl
  21. positive regulation of JNK cascade Source: Ensembl
  22. positive regulation of myoblast differentiation Source: Ensembl
  23. positive regulation of transcription, DNA-templated Source: Ensembl
  24. regulation of fertilization Source: Ensembl
  25. regulation of G-protein coupled receptor protein signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_202864. Acetylcholine regulates insulin secretion.
REACT_209521. G alpha (q) signalling events.
REACT_209727. Ca2+ pathway.
REACT_213422. PLC beta mediated events.
REACT_216130. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
REACT_217228. Presynaptic function of Kainate receptors.
REACT_224596. G beta:gamma signalling through PLC beta.
REACT_225102. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 (EC:3.1.4.11)
Alternative name(s):
PLC-154
Phosphoinositide phospholipase C-beta-1
Phospholipase C-beta-1
Short name:
PLC-beta-1
Gene namesi
Name:PLCB1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 13

Subcellular locationi

Nucleus membrane By similarity. Cytoplasm By similarity
Note: Colocalizes with the adrenergic receptors, ADREN1A and ADREN1B, at the nuclear membrane of cardiac myocytes By similarity.

GO - Cellular componenti

  1. cytoplasm Source: AgBase
  2. cytosol Source: Reactome
  3. nuclear chromatin Source: Ensembl
  4. nuclear membrane Source: UniProtKB-SubCell
  5. nuclear speck Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 121612161-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1PRO_0000088485Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei887 – 8871Phosphoserine; by PKC1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP10894.

Interactioni

Subunit structurei

Interacts with DGKQ By similarity.

Protein-protein interaction databases

BioGridi160009. 1 interaction.
MINTiMINT-144720.
STRINGi9913.ENSBTAP00000046644.

Structurei

3D structure databases

ProteinModelPortaliP10894.
SMRiP10894. Positions 18-797.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini316 – 467152PI-PLC X-boxAdd
BLAST
Domaini540 – 656117PI-PLC Y-boxAdd
BLAST
Domaini663 – 76199C2Add
BLAST

Sequence similaritiesi

Contains 1 C2 domain.

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00730000110266.
HOGENOMiHOG000232046.
HOVERGENiHBG053609.
InParanoidiP10894.
KOiK05858.
OMAiMMDFINL.
OrthoDBiEOG7WDN1N.
TreeFamiTF313216.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028400. PLC-beta1.
IPR014815. PLC-beta_C.
IPR009535. PLC-beta_CS.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF12. PTHR10336:SF12. 1 hit.
PfamiPF06631. DUF1154. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000956. PLC-beta. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10894-1 [UniParc]FASTAAdd to Basket

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MAGAQPGVHA LQLKPVCVSD SLKKGTKFVK WEDDSTVVTP IILRTDPQGF     50
FFYWTDQNKE TELLDLSLVK DARCGKHAKA PKDPKLRELL DVGNIGRLEH 100
RMITVVYGPD LVNISHLNLV AFQEEVAKEW TNEVFSLATN LLAQNMSRDA 150
FLEKAYTKLK LQVTPEGRIP LKNIYRLFSA DRKRVETALE ACSLPSSRND 200
SIPQEDFTPE VYRVFLNNLC PRPEIDNIFS EFGAKSKPYL TVDQMMDFIN 250
LKQRDPRLNE ILYPPLKQEQ VQVLIEKYEP NNSLAKKGQI SVDGFMRYLS 300
GEENGVVSPE KLDLNEDMSQ PLSHYFINSS HNTYLTAGQL AGNSSVEMYR 350
QVLLSGCRCV ELDCWKGRTA EEEPVITHGF TMTTEISFKE VIEAIAECAF 400
KTSPFPILLS FENHVDSPKQ QAKMAEYCRL IFGDALLMEP LDKYPLESGV 450
PLPSPMDLMY KILVKNKKKS HKSSEGSGKK KLSEQASNTY SDSSSVFEPS 500
SPGAGEADTE SDDDDDDDDC KKSSMDEGTA GSEAMATEEM SNLVNYIQPV 550
KFESFEISKK RNRSFEMSSF VETKGLEQLT KSPVEFVEYN KMQLSRIYPK 600
GTRVDSSNYM PQLFWNAGCQ MVALNFQTVD LAMQINMGMY EYNGKSGYRL 650
KPEFMRRPDK HFDPFTEGIV DGIVANTLSV KIISGQFLSD KKVGTYVEVD 700
MFGLPVDTRR KAFKTKTSQG NAVNPIWEEE PIVFKKVVLP SLACLRIAVY 750
EEGGKFIGHR ILPVQAIRPG YHYICLRNER NQPLMLPALF VYIEVKDYVP 800
DTYADVIEAL SNPIRYVNLM EQRAKQLAAL TLEDEEEVKK EADPGETPSE 850
APSEARPTPA ENGVNHTTSL TPKPPSQALH SQPAPGSVKA PAKTEDLIQS 900
VLTEVEAQTI EELKQQKSFV KLQKKHYKEM KDLVKRHHKK TTDLIKEHTT 950
KYNEIQNDYL RRRAALEKTA KKDNKKKSEP SSPDHVSSTI EQDLAALDAE 1000
MTQKLVDLKD KQQQQLLNLR QEQYYSEKYQ KREHIKLLIQ KLTDVAEECQ 1050
NNQLKKLKEI CEKEKKELKK KMDKKRQEKI TEAKSKDKSQ MEEEKTEMIR 1100
SYIQEVVQYI KRLEEAQSKR QEKLVEKHKE IRQQILDEKP KLQVELEQEY 1150
QDKFKRLPLE ILEFVQEAMK GKISEDSNHS SAPPLMTSDS GKLNQKPPSS 1200
EELEGENPGK EFDTPL 1216
Length:1,216
Mass (Da):138,715
Last modified:July 1, 1989 - v1
Checksum:iBEF809177F1B7ABB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03137 mRNA. Translation: AAA30702.1.
PIRiA28822.
RefSeqiNP_777242.1. NM_174817.1.
UniGeneiBt.448.

Genome annotation databases

EnsembliENSBTAT00000049812; ENSBTAP00000046644; ENSBTAG00000008338.
GeneIDi287026.
KEGGibta:287026.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03137 mRNA. Translation: AAA30702.1 .
PIRi A28822.
RefSeqi NP_777242.1. NM_174817.1.
UniGenei Bt.448.

3D structure databases

ProteinModelPortali P10894.
SMRi P10894. Positions 18-797.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 160009. 1 interaction.
MINTi MINT-144720.
STRINGi 9913.ENSBTAP00000046644.

Proteomic databases

PRIDEi P10894.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000049812 ; ENSBTAP00000046644 ; ENSBTAG00000008338 .
GeneIDi 287026.
KEGGi bta:287026.

Organism-specific databases

CTDi 23236.

Phylogenomic databases

eggNOGi NOG149692.
GeneTreei ENSGT00730000110266.
HOGENOMi HOG000232046.
HOVERGENi HBG053609.
InParanoidi P10894.
KOi K05858.
OMAi MMDFINL.
OrthoDBi EOG7WDN1N.
TreeFami TF313216.

Enzyme and pathway databases

Reactomei REACT_202864. Acetylcholine regulates insulin secretion.
REACT_209521. G alpha (q) signalling events.
REACT_209727. Ca2+ pathway.
REACT_213422. PLC beta mediated events.
REACT_216130. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
REACT_217228. Presynaptic function of Kainate receptors.
REACT_224596. G beta:gamma signalling through PLC beta.
REACT_225102. Synthesis of IP3 and IP4 in the cytosol.

Miscellaneous databases

NextBioi 20806548.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028400. PLC-beta1.
IPR014815. PLC-beta_C.
IPR009535. PLC-beta_CS.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
PTHR10336:SF12. PTHR10336:SF12. 1 hit.
Pfami PF06631. DUF1154. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF000956. PLC-beta. 1 hit.
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Determination of the primary structure of PLC-154 demonstrates diversity of phosphoinositide-specific phospholipase C activities."
    Katan M., Kriz R.W., Totty N., Philp R., Meldrum E., Aldape R.A., Knopf J.L., Parker P.J.
    Cell 54:171-177(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "Feedback regulation of phospholipase C-beta by protein kinase C."
    Ryu S.H., Kim U.H., Wahl M.I., Brown A.B., Carpenter G., Huang K.P., Rhee S.G.
    J. Biol. Chem. 265:17941-17945(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 879-889, PHOSPHORYLATION AT SER-887.

Entry informationi

Entry nameiPLCB1_BOVIN
AccessioniPrimary (citable) accession number: P10894
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: September 3, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The receptor-mediated activation of PLC-beta-1 is mediated by two G-protein alpha subunits, alpha-Q and alpha-11.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi