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P10894

- PLCB1_BOVIN

UniProt

P10894 - PLCB1_BOVIN

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1

Gene

PLCB1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Calcium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei331 – 3311PROSITE-ProRule annotation
    Active sitei378 – 3781PROSITE-ProRule annotation

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. GTPase activator activity Source: Ensembl
    3. phosphatidylinositol-4,5-bisphosphate binding Source: Ensembl
    4. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    5. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. activation of meiosis involved in egg activation Source: Ensembl
    2. cerebral cortex development Source: Ensembl
    3. fat cell differentiation Source: Ensembl
    4. glutamate receptor signaling pathway Source: Ensembl
    5. G-protein coupled acetylcholine receptor signaling pathway Source: Ensembl
    6. insulin-like growth factor receptor signaling pathway Source: Ensembl
    7. interleukin-12-mediated signaling pathway Source: Ensembl
    8. interleukin-15-mediated signaling pathway Source: Ensembl
    9. interleukin-1-mediated signaling pathway Source: Ensembl
    10. intracellular signal transduction Source: InterPro
    11. lipid catabolic process Source: UniProtKB-KW
    12. memory Source: Ensembl
    13. negative regulation of monocyte extravasation Source: Ensembl
    14. negative regulation of transcription, DNA-templated Source: Ensembl
    15. positive regulation of acrosome reaction Source: Ensembl
    16. positive regulation of CD24 biosynthetic process Source: Ensembl
    17. positive regulation of developmental growth Source: Ensembl
    18. positive regulation of embryonic development Source: Ensembl
    19. positive regulation of G1/S transition of mitotic cell cycle Source: Ensembl
    20. positive regulation of interleukin-12 production Source: Ensembl
    21. positive regulation of JNK cascade Source: Ensembl
    22. positive regulation of myoblast differentiation Source: Ensembl
    23. positive regulation of transcription, DNA-templated Source: Ensembl
    24. regulation of fertilization Source: Ensembl
    25. regulation of G-protein coupled receptor protein signaling pathway Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium

    Enzyme and pathway databases

    ReactomeiREACT_202864. Acetylcholine regulates insulin secretion.
    REACT_209521. G alpha (q) signalling events.
    REACT_209727. Ca2+ pathway.
    REACT_213422. PLC beta mediated events.
    REACT_216130. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
    REACT_217228. Presynaptic function of Kainate receptors.
    REACT_224596. G beta:gamma signalling through PLC beta.
    REACT_225102. Synthesis of IP3 and IP4 in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 (EC:3.1.4.11)
    Alternative name(s):
    PLC-154
    Phosphoinositide phospholipase C-beta-1
    Phospholipase C-beta-1
    Short name:
    PLC-beta-1
    Gene namesi
    Name:PLCB1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 13

    Subcellular locationi

    Nucleus membrane By similarity. Cytoplasm By similarity
    Note: Colocalizes with the adrenergic receptors, ADREN1A and ADREN1B, at the nuclear membrane of cardiac myocytes.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: AgBase
    2. cytosol Source: Reactome
    3. nuclear chromatin Source: Ensembl
    4. nuclear membrane Source: UniProtKB-SubCell
    5. nuclear speck Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 121612161-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1PRO_0000088485Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei887 – 8871Phosphoserine; by PKC1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP10894.

    Interactioni

    Subunit structurei

    Interacts with DGKQ.By similarity

    Protein-protein interaction databases

    BioGridi160009. 1 interaction.
    MINTiMINT-144720.
    STRINGi9913.ENSBTAP00000046644.

    Structurei

    3D structure databases

    ProteinModelPortaliP10894.
    SMRiP10894. Positions 18-797.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini316 – 467152PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini540 – 656117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini663 – 76199C2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149692.
    GeneTreeiENSGT00730000110266.
    HOGENOMiHOG000232046.
    HOVERGENiHBG053609.
    InParanoidiP10894.
    KOiK05858.
    OMAiMMDFINL.
    OrthoDBiEOG7WDN1N.
    TreeFamiTF313216.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR016280. PLC-beta.
    IPR028400. PLC-beta1.
    IPR014815. PLC-beta_C.
    IPR009535. PLC-beta_CS.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF12. PTHR10336:SF12. 1 hit.
    PfamiPF06631. DUF1154. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    PF08703. PLC-beta_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000956. PLC-beta. 1 hit.
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P10894-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGAQPGVHA LQLKPVCVSD SLKKGTKFVK WEDDSTVVTP IILRTDPQGF     50
    FFYWTDQNKE TELLDLSLVK DARCGKHAKA PKDPKLRELL DVGNIGRLEH 100
    RMITVVYGPD LVNISHLNLV AFQEEVAKEW TNEVFSLATN LLAQNMSRDA 150
    FLEKAYTKLK LQVTPEGRIP LKNIYRLFSA DRKRVETALE ACSLPSSRND 200
    SIPQEDFTPE VYRVFLNNLC PRPEIDNIFS EFGAKSKPYL TVDQMMDFIN 250
    LKQRDPRLNE ILYPPLKQEQ VQVLIEKYEP NNSLAKKGQI SVDGFMRYLS 300
    GEENGVVSPE KLDLNEDMSQ PLSHYFINSS HNTYLTAGQL AGNSSVEMYR 350
    QVLLSGCRCV ELDCWKGRTA EEEPVITHGF TMTTEISFKE VIEAIAECAF 400
    KTSPFPILLS FENHVDSPKQ QAKMAEYCRL IFGDALLMEP LDKYPLESGV 450
    PLPSPMDLMY KILVKNKKKS HKSSEGSGKK KLSEQASNTY SDSSSVFEPS 500
    SPGAGEADTE SDDDDDDDDC KKSSMDEGTA GSEAMATEEM SNLVNYIQPV 550
    KFESFEISKK RNRSFEMSSF VETKGLEQLT KSPVEFVEYN KMQLSRIYPK 600
    GTRVDSSNYM PQLFWNAGCQ MVALNFQTVD LAMQINMGMY EYNGKSGYRL 650
    KPEFMRRPDK HFDPFTEGIV DGIVANTLSV KIISGQFLSD KKVGTYVEVD 700
    MFGLPVDTRR KAFKTKTSQG NAVNPIWEEE PIVFKKVVLP SLACLRIAVY 750
    EEGGKFIGHR ILPVQAIRPG YHYICLRNER NQPLMLPALF VYIEVKDYVP 800
    DTYADVIEAL SNPIRYVNLM EQRAKQLAAL TLEDEEEVKK EADPGETPSE 850
    APSEARPTPA ENGVNHTTSL TPKPPSQALH SQPAPGSVKA PAKTEDLIQS 900
    VLTEVEAQTI EELKQQKSFV KLQKKHYKEM KDLVKRHHKK TTDLIKEHTT 950
    KYNEIQNDYL RRRAALEKTA KKDNKKKSEP SSPDHVSSTI EQDLAALDAE 1000
    MTQKLVDLKD KQQQQLLNLR QEQYYSEKYQ KREHIKLLIQ KLTDVAEECQ 1050
    NNQLKKLKEI CEKEKKELKK KMDKKRQEKI TEAKSKDKSQ MEEEKTEMIR 1100
    SYIQEVVQYI KRLEEAQSKR QEKLVEKHKE IRQQILDEKP KLQVELEQEY 1150
    QDKFKRLPLE ILEFVQEAMK GKISEDSNHS SAPPLMTSDS GKLNQKPPSS 1200
    EELEGENPGK EFDTPL 1216
    Length:1,216
    Mass (Da):138,715
    Last modified:July 1, 1989 - v1
    Checksum:iBEF809177F1B7ABB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03137 mRNA. Translation: AAA30702.1.
    PIRiA28822.
    RefSeqiNP_777242.1. NM_174817.1.
    UniGeneiBt.448.

    Genome annotation databases

    EnsembliENSBTAT00000049812; ENSBTAP00000046644; ENSBTAG00000008338.
    GeneIDi287026.
    KEGGibta:287026.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03137 mRNA. Translation: AAA30702.1 .
    PIRi A28822.
    RefSeqi NP_777242.1. NM_174817.1.
    UniGenei Bt.448.

    3D structure databases

    ProteinModelPortali P10894.
    SMRi P10894. Positions 18-797.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 160009. 1 interaction.
    MINTi MINT-144720.
    STRINGi 9913.ENSBTAP00000046644.

    Proteomic databases

    PRIDEi P10894.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000049812 ; ENSBTAP00000046644 ; ENSBTAG00000008338 .
    GeneIDi 287026.
    KEGGi bta:287026.

    Organism-specific databases

    CTDi 23236.

    Phylogenomic databases

    eggNOGi NOG149692.
    GeneTreei ENSGT00730000110266.
    HOGENOMi HOG000232046.
    HOVERGENi HBG053609.
    InParanoidi P10894.
    KOi K05858.
    OMAi MMDFINL.
    OrthoDBi EOG7WDN1N.
    TreeFami TF313216.

    Enzyme and pathway databases

    Reactomei REACT_202864. Acetylcholine regulates insulin secretion.
    REACT_209521. G alpha (q) signalling events.
    REACT_209727. Ca2+ pathway.
    REACT_213422. PLC beta mediated events.
    REACT_216130. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
    REACT_217228. Presynaptic function of Kainate receptors.
    REACT_224596. G beta:gamma signalling through PLC beta.
    REACT_225102. Synthesis of IP3 and IP4 in the cytosol.

    Miscellaneous databases

    NextBioi 20806548.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR016280. PLC-beta.
    IPR028400. PLC-beta1.
    IPR014815. PLC-beta_C.
    IPR009535. PLC-beta_CS.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF12. PTHR10336:SF12. 1 hit.
    Pfami PF06631. DUF1154. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    PF08703. PLC-beta_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000956. PLC-beta. 1 hit.
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Determination of the primary structure of PLC-154 demonstrates diversity of phosphoinositide-specific phospholipase C activities."
      Katan M., Kriz R.W., Totty N., Philp R., Meldrum E., Aldape R.A., Knopf J.L., Parker P.J.
      Cell 54:171-177(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. "Feedback regulation of phospholipase C-beta by protein kinase C."
      Ryu S.H., Kim U.H., Wahl M.I., Brown A.B., Carpenter G., Huang K.P., Rhee S.G.
      J. Biol. Chem. 265:17941-17945(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 879-889, PHOSPHORYLATION AT SER-887.

    Entry informationi

    Entry nameiPLCB1_BOVIN
    AccessioniPrimary (citable) accession number: P10894
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The receptor-mediated activation of PLC-beta-1 is mediated by two G-protein alpha subunits, alpha-Q and alpha-11.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3