P10891 (DEFI_PROTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Phormicin Alternative name(s): Insect defensin A/B
Organism	Protophormia terraenovae (Northern blowfly) (Lucilia terraenovae)
Taxonomic identifier	34676 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Calyptratae › Oestroidea › Calliphoridae › Chrysomyinae › Phormini › Protophormia

Protein attributes

Sequence length	94 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the anti Gram-positive activity of immune hemolymph of P.terraenovae.
Subcellular location	Secreted.
Sequence similarities	Belongs to the invertebrate defensin family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Immunity Innate immunity
Cellular component	Secreted
Domain	Signal
Molecular function	Antibiotic Antimicrobial Defensin
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

23

Potential

Propeptide

24 – 54

31

PRO_0000006748

Chain

55 – 94

40

Phormicin

PRO_0000006749

Amino acid modifications

Disulfide bond

57 ↔ 84

Ref.3

Disulfide bond

70 ↔ 90

Ref.3

Disulfide bond

74 ↔ 92

Ref.3

Natural variations

Natural variant

86

1

G → R in defensin B.

Secondary structure

1

.

.

.

.

.

94

Helix Strand Turn

Details...

Helix

68 – 77

10

Beta strand

86 – 88

3

Sequences

	Sequence		Length	Mass (Da)
	P10891 [UniParc]. Last modified November 1, 1991. Version 2. Checksum: CE24ED83D7CBE6D9	FASTA	94	10,110
	10 20 30 40 50 60 MKFFMVFVVT FCLAVCFVSQ SLAIPADAAN DAHFVDGVQA LKEIEPELHG RYKRATCDLL 70 80 90 SGTGINHSAC AAHCLLRGNR GGYCNGKGVC VCRN

References

[1]	"Insect immunity: expression of the two major inducible antibacterial peptides, defensin and diptericin, in Phormia terranovae." Dimarcq J.-L., Zachary D., Hoffmann J.A., Hoffmann D., Reichhart J.-M. EMBO J. 9:2507-2515(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Insect immunity: isolation from immune blood of the dipteran Phormia terranovae of two insect antibacterial peptides with sequence homology to rabbit lung macrophage bactericidal peptides." Lambert J., Keppi E., Dimarcq J.-L., Wicker C., Reichhart J.-M., Dunbar B., Lepage P., van Dorsselaer A., Hoffmann J.A., Fothergill J., Hoffmann D. Proc. Natl. Acad. Sci. U.S.A. 86:262-266(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 55-94. Tissue: Hemolymph.
[3]	"Determination of disulfide bridges in natural and recombinant insect defensin A." Lepage P., Bitsch F., Roecklin D., Keppi E., Dimarcq J.-L., Reichhart J.-M., Hoffmann J.A., Roitsch C., van Dorsselaer A. Eur. J. Biochem. 196:735-742(1991) [PubMed] [Europe PMC] [Abstract] Cited for: DISULFIDE BONDS.
[4]	"Progress in multidimensional NMR investigations of peptide and protein 3-D structures in solution. From structure to functional aspects." Bonmatin J.-M., Genest M., Petit M.-C., Gincel E., Simorre J.-P., Cornet B., Gallet X., Caille A., Labbe H., Vovelle F., Ptak M. Biochimie 74:825-836(1992) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[5]	"Refined three-dimensional solution structure of insect defensin A." Cornet B., Bonmatin J.-M., Hetru C., Hoffmann J.A., Ptak M., Vovelle F. Structure 3:435-448(1995) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 55-94.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X55546 mRNA. Translation: CAA39152.1.

PIR

S12558.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ICA	NMR	-	A	55-94	[»]

ProteinModelPortal

P10891.

SMR

P10891. Positions 55-94.

ModBase

Search...

Protein family/group databases

TCDB

1.C.47.1.2. insect/fungal defensin (Insect/fungal Defensin) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.30.30.10. 1 hit.

InterPro

IPR017982. Defensin_insect.
IPR001542. Defensin_invertebrate/fungal.
IPR003614. Scorpion_toxin-like.
[Graphical view]

Pfam

PF01097. Defensin_2. 1 hit.
[Graphical view]

PRINTS

PR00271. DEFENSIN.

SMART

SM00505. Knot1. 1 hit.
[Graphical view]

SUPFAM

SSF57095. SSF57095. 1 hit.

PROSITE

PS51378. INVERT_DEFENSINS. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P10891.

Entry information

Entry name

DEFI_PROTE

Accession

Primary (citable) accession number: P10891

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	November 1, 1991
Last modified:	May 1, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)