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Protein

High-affinity glucose transporter SNF3

Gene

SNF3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity glucose transporter. Can function as a negative regulator of glucose transport. SNF3 is involved as well in the transport of mannose and fructose. Serves as a sensor that generates an intracellular signal in the presence of low level of glucose. Inhibition of RGT1 by low levels of glucose, and hence induction of HTX2 and HTX4 expression, requires SNF3.

GO - Molecular functioni

  • glucose binding Source: SGD
  • receptor activity Source: SGD
  • sugar:proton symporter activity Source: GO_Central

GO - Biological processi

  • detection of glucose Source: SGD
  • fructose transport Source: SGD
  • glucose mediated signaling pathway Source: SGD
  • glucose transport Source: SGD
  • hexose transmembrane transport Source: GO_Central
  • mannose transport Source: SGD
  • negative regulation of meiotic nuclear division Source: SGD
  • signal transduction Source: SGD
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29579-MONOMER.

Protein family/group databases

TCDBi2.A.1.1.17. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
High-affinity glucose transporter SNF3
Gene namesi
Name:SNF3
Ordered Locus Names:YDL194W
ORF Names:D1234
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL194W.
SGDiS000002353. SNF3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 97CytoplasmicSequence analysisAdd BLAST97
Transmembranei98 – 118Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini119 – 143ExtracellularSequence analysisAdd BLAST25
Transmembranei144 – 164Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini165 – 178CytoplasmicSequence analysisAdd BLAST14
Transmembranei179 – 199Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini200 – 202ExtracellularSequence analysis3
Transmembranei203 – 223Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini224 – 229CytoplasmicSequence analysis6
Transmembranei230 – 250Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini251 – 264ExtracellularSequence analysisAdd BLAST14
Transmembranei265 – 285Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini286 – 358CytoplasmicSequence analysisAdd BLAST73
Transmembranei359 – 379Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini380 – 391ExtracellularSequence analysisAdd BLAST12
Transmembranei392 – 412Helical; Name=8Sequence analysisAdd BLAST21
Topological domaini413 – 419CytoplasmicSequence analysis7
Transmembranei420 – 440Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini441 – 451ExtracellularSequence analysisAdd BLAST11
Transmembranei452 – 472Helical; Name=10Sequence analysisAdd BLAST21
Topological domaini473 – 488CytoplasmicSequence analysisAdd BLAST16
Transmembranei489 – 509Helical; Name=11Sequence analysisAdd BLAST21
Topological domaini510 – 521ExtracellularSequence analysisAdd BLAST12
Transmembranei522 – 542Helical; Name=12Sequence analysisAdd BLAST21
Topological domaini543 – 884CytoplasmicSequence analysisAdd BLAST342

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi112G → D in SNF3-142. 1 Publication1
Mutagenesisi153G → R in SNF3-72. 1 Publication1
Mutagenesisi229R → K in SNF3-1; constitutively signaling glucose receptor. 1 Publication1
Mutagenesisi402V → I in SNF3-39. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000503901 – 884High-affinity glucose transporter SNF3Add BLAST884

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi383N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP10870.

PTM databases

iPTMnetiP10870.

Expressioni

Inductioni

Glucose-repressible.

Interactioni

Protein-protein interaction databases

BioGridi31851. 30 interactors.
IntActiP10870. 2 interactors.

Structurei

3D structure databases

ProteinModelPortaliP10870.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The C-terminal cytoplasmic domain seems to be important for SNF3 regulatory function.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00850000132516.
HOGENOMiHOG000202870.
InParanoidiP10870.
KOiK08139.
OMAiWLVNFIC.
OrthoDBiEOG092C1Q5W.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10870-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPNSNSSSE TLRQEKQGFL DKALQRVKGI ALRRNNSNKD HTTDDTTGSI
60 70 80 90 100
RTPTSLQRQN SDRQSNMTSV FTDDISTIDD NSILFSEPPQ KQSMMMSICV
110 120 130 140 150
GVFVAVGGFL FGYDTGLINS ITSMNYVKSH VAPNHDSFTA QQMSILVSFL
160 170 180 190 200
SLGTFFGALT APFISDSYGR KPTIIFSTIF IFSIGNSLQV GAGGITLLIV
210 220 230 240 250
GRVISGIGIG AISAVVPLYQ AEATHKSLRG AIISTYQWAI TWGLLVSSAV
260 270 280 290 300
SQGTHARNDA SSYRIPIGLQ YVWSSFLAIG MFFLPESPRY YVLKDKLDEA
310 320 330 340 350
AKSLSFLRGV PVHDSGLLEE LVEIKATYDY EASFGSSNFI DCFISSKSRP
360 370 380 390 400
KQTLRMFTGI ALQAFQQFSG INFIFYYGVN FFNKTGVSNS YLVSFITYAV
410 420 430 440 450
NVVFNVPGLF FVEFFGRRKV LVVGGVIMTI ANFIVAIVGC SLKTVAAAKV
460 470 480 490 500
MIAFICLFIA AFSATWGGVV WVISAELYPL GVRSKCTAIC AAANWLVNFI
510 520 530 540 550
CALITPYIVD TGSHTSSLGA KIFFIWGSLN AMGVIVVYLT VYETKGLTLE
560 570 580 590 600
EIDELYIKSS TGVVSPKFNK DIRERALKFQ YDPLQRLEDG KNTFVAKRNN
610 620 630 640 650
FDDETPRNDF RNTISGEIDH SPNQKEVHSI PERVDIPTST EILESPNKSS
660 670 680 690 700
GMTVPVSPSL QDVPIPQTTE PAEIRTKYVD LGNGLGLNTY NRGPPSLSSD
710 720 730 740 750
SSEDYTEDEI GGPSSQGDQS NRSTMNDIND YMARLIHSTS TASNTTDKFS
760 770 780 790 800
GNQSTLRYHT ASSHSDTTEE DSNLMDLGNG LALNAYNRGP PSILMNSSDE
810 820 830 840 850
EANGGETSDN LNTAQDLAGM KERMAQFAQS YIDKRGGLEP ETQSNILSTS
860 870 880
LSVMADTNEH NNEILHSSEE NATNQPVNEN NDLK
Length:884
Mass (Da):96,719
Last modified:October 3, 2006 - v3
Checksum:i8E2A5A1A7739344F
GO

Sequence cautioni

The sequence CAA58253 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03246 Genomic DNA. Translation: AAA35060.1.
X83276 Genomic DNA. Translation: CAA58253.1. Different initiation.
Z74242 Genomic DNA. Translation: CAA98771.1.
BK006938 Genomic DNA. Translation: DAA11669.1.
PIRiA31928.
RefSeqiNP_010087.1. NM_001180254.1.

Genome annotation databases

EnsemblFungiiYDL194W; YDL194W; YDL194W.
GeneIDi851333.
KEGGisce:YDL194W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03246 Genomic DNA. Translation: AAA35060.1.
X83276 Genomic DNA. Translation: CAA58253.1. Different initiation.
Z74242 Genomic DNA. Translation: CAA98771.1.
BK006938 Genomic DNA. Translation: DAA11669.1.
PIRiA31928.
RefSeqiNP_010087.1. NM_001180254.1.

3D structure databases

ProteinModelPortaliP10870.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31851. 30 interactors.
IntActiP10870. 2 interactors.

Protein family/group databases

TCDBi2.A.1.1.17. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiP10870.

Proteomic databases

PRIDEiP10870.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL194W; YDL194W; YDL194W.
GeneIDi851333.
KEGGisce:YDL194W.

Organism-specific databases

EuPathDBiFungiDB:YDL194W.
SGDiS000002353. SNF3.

Phylogenomic databases

GeneTreeiENSGT00850000132516.
HOGENOMiHOG000202870.
InParanoidiP10870.
KOiK08139.
OMAiWLVNFIC.
OrthoDBiEOG092C1Q5W.

Enzyme and pathway databases

BioCyciYEAST:G3O-29579-MONOMER.

Miscellaneous databases

PROiP10870.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSNF3_YEAST
AccessioniPrimary (citable) accession number: P10870
Secondary accession number(s): D6VRF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 3, 2006
Last modified: November 30, 2016
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Glucose transport is thought to be mediated by two kinetically distinct systems, a glucose-repressible high-affinity system and a constitutive low-affinity system.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.