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P10870

- SNF3_YEAST

UniProt

P10870 - SNF3_YEAST

Protein

High-affinity glucose transporter SNF3

Gene

SNF3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 3 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    High-affinity glucose transporter. Can function as a negative regulator of glucose transport. SNF3 is involved as well in the transport of mannose and fructose. Serves as a sensor that generates an intracellular signal in the presence of low level of glucose. Inhibition of RGT1 by low levels of glucose, and hence induction of HTX2 and HTX4 expression, requires SNF3.

    GO - Molecular functioni

    1. glucose binding Source: SGD
    2. receptor activity Source: SGD
    3. substrate-specific transmembrane transporter activity Source: InterPro

    GO - Biological processi

    1. detection of glucose Source: SGD
    2. fructose transport Source: SGD
    3. glucose transport Source: SGD
    4. mannose transport Source: SGD
    5. negative regulation of meiosis Source: SGD
    6. signal transduction Source: SGD

    Keywords - Biological processi

    Sugar transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29579-MONOMER.

    Protein family/group databases

    TCDBi2.A.1.1.17. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    High-affinity glucose transporter SNF3
    Gene namesi
    Name:SNF3
    Ordered Locus Names:YDL194W
    ORF Names:D1234
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL194w.
    SGDiS000002353. SNF3.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi112 – 1121G → D in SNF3-142. 1 Publication
    Mutagenesisi153 – 1531G → R in SNF3-72. 1 Publication
    Mutagenesisi229 – 2291R → K in SNF3-1; constitutively signaling glucose receptor. 1 Publication
    Mutagenesisi402 – 4021V → I in SNF3-39. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 884884High-affinity glucose transporter SNF3PRO_0000050390Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi383 – 3831N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Inductioni

    Glucose-repressible.

    Gene expression databases

    GenevestigatoriP10870.

    Interactioni

    Protein-protein interaction databases

    BioGridi31851. 30 interactions.
    IntActiP10870. 2 interactions.
    STRINGi4932.YDL194W.

    Structurei

    3D structure databases

    ProteinModelPortaliP10870.
    SMRiP10870. Positions 100-556.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9797CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini119 – 14325ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini165 – 17814CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini200 – 2023ExtracellularSequence Analysis
    Topological domaini224 – 2296CytoplasmicSequence Analysis
    Topological domaini251 – 26414ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini286 – 35873CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini380 – 39112ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini413 – 4197CytoplasmicSequence Analysis
    Topological domaini441 – 45111ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini473 – 48816CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini510 – 52112ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini543 – 884342CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei98 – 11821Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei144 – 16421Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei179 – 19921Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei203 – 22321Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei230 – 25021Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei265 – 28521Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei359 – 37921Helical; Name=7Sequence AnalysisAdd
    BLAST
    Transmembranei392 – 41221Helical; Name=8Sequence AnalysisAdd
    BLAST
    Transmembranei420 – 44021Helical; Name=9Sequence AnalysisAdd
    BLAST
    Transmembranei452 – 47221Helical; Name=10Sequence AnalysisAdd
    BLAST
    Transmembranei489 – 50921Helical; Name=11Sequence AnalysisAdd
    BLAST
    Transmembranei522 – 54221Helical; Name=12Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domaini

    The C-terminal cytoplasmic domain seems to be important for SNF3 regulatory function.

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    GeneTreeiENSGT00720000108754.
    HOGENOMiHOG000202870.
    KOiK08139.
    OrthoDBiEOG73JM4H.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR005828. Sub_transporter.
    IPR003663. Sugar/inositol_transpt.
    IPR005829. Sugar_transporter_CS.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    PRINTSiPR00171. SUGRTRNSPORT.
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00879. SP. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    PS00217. SUGAR_TRANSPORT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P10870-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPNSNSSSE TLRQEKQGFL DKALQRVKGI ALRRNNSNKD HTTDDTTGSI    50
    RTPTSLQRQN SDRQSNMTSV FTDDISTIDD NSILFSEPPQ KQSMMMSICV 100
    GVFVAVGGFL FGYDTGLINS ITSMNYVKSH VAPNHDSFTA QQMSILVSFL 150
    SLGTFFGALT APFISDSYGR KPTIIFSTIF IFSIGNSLQV GAGGITLLIV 200
    GRVISGIGIG AISAVVPLYQ AEATHKSLRG AIISTYQWAI TWGLLVSSAV 250
    SQGTHARNDA SSYRIPIGLQ YVWSSFLAIG MFFLPESPRY YVLKDKLDEA 300
    AKSLSFLRGV PVHDSGLLEE LVEIKATYDY EASFGSSNFI DCFISSKSRP 350
    KQTLRMFTGI ALQAFQQFSG INFIFYYGVN FFNKTGVSNS YLVSFITYAV 400
    NVVFNVPGLF FVEFFGRRKV LVVGGVIMTI ANFIVAIVGC SLKTVAAAKV 450
    MIAFICLFIA AFSATWGGVV WVISAELYPL GVRSKCTAIC AAANWLVNFI 500
    CALITPYIVD TGSHTSSLGA KIFFIWGSLN AMGVIVVYLT VYETKGLTLE 550
    EIDELYIKSS TGVVSPKFNK DIRERALKFQ YDPLQRLEDG KNTFVAKRNN 600
    FDDETPRNDF RNTISGEIDH SPNQKEVHSI PERVDIPTST EILESPNKSS 650
    GMTVPVSPSL QDVPIPQTTE PAEIRTKYVD LGNGLGLNTY NRGPPSLSSD 700
    SSEDYTEDEI GGPSSQGDQS NRSTMNDIND YMARLIHSTS TASNTTDKFS 750
    GNQSTLRYHT ASSHSDTTEE DSNLMDLGNG LALNAYNRGP PSILMNSSDE 800
    EANGGETSDN LNTAQDLAGM KERMAQFAQS YIDKRGGLEP ETQSNILSTS 850
    LSVMADTNEH NNEILHSSEE NATNQPVNEN NDLK 884
    Length:884
    Mass (Da):96,719
    Last modified:October 3, 2006 - v3
    Checksum:i8E2A5A1A7739344F
    GO

    Sequence cautioni

    The sequence CAA58253.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03246 Genomic DNA. Translation: AAA35060.1.
    X83276 Genomic DNA. Translation: CAA58253.1. Different initiation.
    Z74242 Genomic DNA. Translation: CAA98771.1.
    BK006938 Genomic DNA. Translation: DAA11669.1.
    PIRiA31928.
    RefSeqiNP_010087.1. NM_001180254.1.

    Genome annotation databases

    EnsemblFungiiYDL194W; YDL194W; YDL194W.
    GeneIDi851333.
    KEGGisce:YDL194W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03246 Genomic DNA. Translation: AAA35060.1 .
    X83276 Genomic DNA. Translation: CAA58253.1 . Different initiation.
    Z74242 Genomic DNA. Translation: CAA98771.1 .
    BK006938 Genomic DNA. Translation: DAA11669.1 .
    PIRi A31928.
    RefSeqi NP_010087.1. NM_001180254.1.

    3D structure databases

    ProteinModelPortali P10870.
    SMRi P10870. Positions 100-556.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31851. 30 interactions.
    IntActi P10870. 2 interactions.
    STRINGi 4932.YDL194W.

    Protein family/group databases

    TCDBi 2.A.1.1.17. the major facilitator superfamily (mfs).

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL194W ; YDL194W ; YDL194W .
    GeneIDi 851333.
    KEGGi sce:YDL194W.

    Organism-specific databases

    CYGDi YDL194w.
    SGDi S000002353. SNF3.

    Phylogenomic databases

    eggNOGi COG0477.
    GeneTreei ENSGT00720000108754.
    HOGENOMi HOG000202870.
    KOi K08139.
    OrthoDBi EOG73JM4H.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29579-MONOMER.

    Miscellaneous databases

    NextBioi 968396.

    Gene expression databases

    Genevestigatori P10870.

    Family and domain databases

    InterProi IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR005828. Sub_transporter.
    IPR003663. Sugar/inositol_transpt.
    IPR005829. Sugar_transporter_CS.
    [Graphical view ]
    Pfami PF00083. Sugar_tr. 1 hit.
    [Graphical view ]
    PRINTSi PR00171. SUGRTRNSPORT.
    SUPFAMi SSF103473. SSF103473. 2 hits.
    TIGRFAMsi TIGR00879. SP. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    PS00217. SUGAR_TRANSPORT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The yeast SNF3 gene encodes a glucose transporter homologous to the mammalian protein."
      Celenza J.L., Marshall-Carlson L., Carlson M.
      Proc. Natl. Acad. Sci. U.S.A. 85:2130-2134(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
    2. "The sequence of 23 kb surrounding the SNF3 locus on the left arm of yeast chromosome IV reveals the location of five known genes and characterizes at least six new open reading frames including putative genes for ribosomal protein L35 and a sugar transport protein."
      Verhasselt P., Voet M., Mathys J., Volckaert G.
      Yeast 12:1065-1070(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Mutational analysis of the SNF3 glucose transporter of Saccharomyces cerevisiae."
      Marshall-Carlson L., Celenza J.L., Laurent B.C., Carlson M.
      Mol. Cell. Biol. 10:1105-1115(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF GLY-112; GLY-153; ARG-229 AND VAL-402.
    6. "The C-terminal domain of Snf3p is sufficient to complement the growth defect of snf3 null mutations in Saccharomyces cerevisiae: SNF3 functions in glucose recognition."
      Coons D.M., Vagnoli P., Bisson L.F.
      Yeast 13:9-20(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSNF3_YEAST
    AccessioniPrimary (citable) accession number: P10870
    Secondary accession number(s): D6VRF9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 128 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Glucose transport is thought to be mediated by two kinetically distinct systems, a glucose-repressible high-affinity system and a constitutive low-affinity system.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3