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P10870 (SNF3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
High-affinity glucose transporter SNF3
Gene names
Name:SNF3
Ordered Locus Names:YDL194W
ORF Names:D1234
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length884 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

High-affinity glucose transporter. Can function as a negative regulator of glucose transport. SNF3 is involved as well in the transport of mannose and fructose. Serves as a sensor that generates an intracellular signal in the presence of low level of glucose. Inhibition of RGT1 by low levels of glucose, and hence induction of HTX2 and HTX4 expression, requires SNF3.

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.1.

Induction

Glucose-repressible.

Domain

The C-terminal cytoplasmic domain seems to be important for SNF3 regulatory function.

Miscellaneous

Glucose transport is thought to be mediated by two kinetically distinct systems, a glucose-repressible high-affinity system and a constitutive low-affinity system.

Sequence similarities

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. [View classification]

Sequence caution

The sequence CAA58253.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 884884High-affinity glucose transporter SNF3
PRO_0000050390

Regions

Topological domain1 – 9797Cytoplasmic Potential
Transmembrane98 – 11821Helical; Name=1; Potential
Topological domain119 – 14325Extracellular Potential
Transmembrane144 – 16421Helical; Name=2; Potential
Topological domain165 – 17814Cytoplasmic Potential
Transmembrane179 – 19921Helical; Name=3; Potential
Topological domain200 – 2023Extracellular Potential
Transmembrane203 – 22321Helical; Name=4; Potential
Topological domain224 – 2296Cytoplasmic Potential
Transmembrane230 – 25021Helical; Name=5; Potential
Topological domain251 – 26414Extracellular Potential
Transmembrane265 – 28521Helical; Name=6; Potential
Topological domain286 – 35873Cytoplasmic Potential
Transmembrane359 – 37921Helical; Name=7; Potential
Topological domain380 – 39112Extracellular Potential
Transmembrane392 – 41221Helical; Name=8; Potential
Topological domain413 – 4197Cytoplasmic Potential
Transmembrane420 – 44021Helical; Name=9; Potential
Topological domain441 – 45111Extracellular Potential
Transmembrane452 – 47221Helical; Name=10; Potential
Topological domain473 – 48816Cytoplasmic Potential
Transmembrane489 – 50921Helical; Name=11; Potential
Topological domain510 – 52112Extracellular Potential
Transmembrane522 – 54221Helical; Name=12; Potential
Topological domain543 – 884342Cytoplasmic Potential

Amino acid modifications

Glycosylation3831N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis1121G → D in SNF3-142. Ref.5
Mutagenesis1531G → R in SNF3-72. Ref.5
Mutagenesis2291R → K in SNF3-1; constitutively signaling glucose receptor. Ref.5
Mutagenesis4021V → I in SNF3-39. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P10870 [UniParc].

Last modified October 3, 2006. Version 3.
Checksum: 8E2A5A1A7739344F

FASTA88496,719
        10         20         30         40         50         60 
MDPNSNSSSE TLRQEKQGFL DKALQRVKGI ALRRNNSNKD HTTDDTTGSI RTPTSLQRQN 

        70         80         90        100        110        120 
SDRQSNMTSV FTDDISTIDD NSILFSEPPQ KQSMMMSICV GVFVAVGGFL FGYDTGLINS 

       130        140        150        160        170        180 
ITSMNYVKSH VAPNHDSFTA QQMSILVSFL SLGTFFGALT APFISDSYGR KPTIIFSTIF 

       190        200        210        220        230        240 
IFSIGNSLQV GAGGITLLIV GRVISGIGIG AISAVVPLYQ AEATHKSLRG AIISTYQWAI 

       250        260        270        280        290        300 
TWGLLVSSAV SQGTHARNDA SSYRIPIGLQ YVWSSFLAIG MFFLPESPRY YVLKDKLDEA 

       310        320        330        340        350        360 
AKSLSFLRGV PVHDSGLLEE LVEIKATYDY EASFGSSNFI DCFISSKSRP KQTLRMFTGI 

       370        380        390        400        410        420 
ALQAFQQFSG INFIFYYGVN FFNKTGVSNS YLVSFITYAV NVVFNVPGLF FVEFFGRRKV 

       430        440        450        460        470        480 
LVVGGVIMTI ANFIVAIVGC SLKTVAAAKV MIAFICLFIA AFSATWGGVV WVISAELYPL 

       490        500        510        520        530        540 
GVRSKCTAIC AAANWLVNFI CALITPYIVD TGSHTSSLGA KIFFIWGSLN AMGVIVVYLT 

       550        560        570        580        590        600 
VYETKGLTLE EIDELYIKSS TGVVSPKFNK DIRERALKFQ YDPLQRLEDG KNTFVAKRNN 

       610        620        630        640        650        660 
FDDETPRNDF RNTISGEIDH SPNQKEVHSI PERVDIPTST EILESPNKSS GMTVPVSPSL 

       670        680        690        700        710        720 
QDVPIPQTTE PAEIRTKYVD LGNGLGLNTY NRGPPSLSSD SSEDYTEDEI GGPSSQGDQS 

       730        740        750        760        770        780 
NRSTMNDIND YMARLIHSTS TASNTTDKFS GNQSTLRYHT ASSHSDTTEE DSNLMDLGNG 

       790        800        810        820        830        840 
LALNAYNRGP PSILMNSSDE EANGGETSDN LNTAQDLAGM KERMAQFAQS YIDKRGGLEP 

       850        860        870        880 
ETQSNILSTS LSVMADTNEH NNEILHSSEE NATNQPVNEN NDLK 

« Hide

References

« Hide 'large scale' references
[1]"The yeast SNF3 gene encodes a glucose transporter homologous to the mammalian protein."
Celenza J.L., Marshall-Carlson L., Carlson M.
Proc. Natl. Acad. Sci. U.S.A. 85:2130-2134(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
[2]"The sequence of 23 kb surrounding the SNF3 locus on the left arm of yeast chromosome IV reveals the location of five known genes and characterizes at least six new open reading frames including putative genes for ribosomal protein L35 and a sugar transport protein."
Verhasselt P., Voet M., Mathys J., Volckaert G.
Yeast 12:1065-1070(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Mutational analysis of the SNF3 glucose transporter of Saccharomyces cerevisiae."
Marshall-Carlson L., Celenza J.L., Laurent B.C., Carlson M.
Mol. Cell. Biol. 10:1105-1115(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF GLY-112; GLY-153; ARG-229 AND VAL-402.
[6]"The C-terminal domain of Snf3p is sufficient to complement the growth defect of snf3 null mutations in Saccharomyces cerevisiae: SNF3 functions in glucose recognition."
Coons D.M., Vagnoli P., Bisson L.F.
Yeast 13:9-20(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[7]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[8]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J03246 Genomic DNA. Translation: AAA35060.1.
X83276 Genomic DNA. Translation: CAA58253.1. Different initiation.
Z74242 Genomic DNA. Translation: CAA98771.1.
BK006938 Genomic DNA. Translation: DAA11669.1.
PIRA31928.
RefSeqNP_010087.1. NM_001180254.1.

3D structure databases

ProteinModelPortalP10870.
SMRP10870. Positions 100-556.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31851. 30 interactions.
IntActP10870. 2 interactions.
STRING4932.YDL194W.

Protein family/group databases

TCDB2.A.1.1.17. the major facilitator superfamily (mfs).

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL194W; YDL194W; YDL194W.
GeneID851333.
KEGGsce:YDL194W.

Organism-specific databases

CYGDYDL194w.
SGDS000002353. SNF3.

Phylogenomic databases

eggNOGCOG0477.
GeneTreeENSGT00720000108754.
HOGENOMHOG000202870.
KOK08139.
OrthoDBEOG73JM4H.

Enzyme and pathway databases

BioCycYEAST:G3O-29579-MONOMER.

Gene expression databases

GenevestigatorP10870.

Family and domain databases

InterProIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSPR00171. SUGRTRNSPORT.
SUPFAMSSF103473. SSF103473. 2 hits.
TIGRFAMsTIGR00879. SP. 1 hit.
PROSITEPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968396.

Entry information

Entry nameSNF3_YEAST
AccessionPrimary (citable) accession number: P10870
Secondary accession number(s): D6VRF9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 3, 2006
Last modified: February 19, 2014
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families