Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

High-affinity glucose transporter SNF3

Gene

SNF3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

High-affinity glucose transporter. Can function as a negative regulator of glucose transport. SNF3 is involved as well in the transport of mannose and fructose. Serves as a sensor that generates an intracellular signal in the presence of low level of glucose. Inhibition of RGT1 by low levels of glucose, and hence induction of HTX2 and HTX4 expression, requires SNF3.

Miscellaneous

Glucose transport is thought to be mediated by two kinetically distinct systems, a glucose-repressible high-affinity system and a constitutive low-affinity system.

GO - Molecular functioni

  • carbohydrate:proton symporter activity Source: GO_Central
  • glucose binding Source: SGD

GO - Biological processi

  • detection of glucose Source: SGD
  • fructose transmembrane transport Source: SGD
  • glucose mediated signaling pathway Source: SGD
  • glucose transmembrane transport Source: SGD
  • mannose transmembrane transport Source: SGD
  • negative regulation of meiotic nuclear division Source: SGD
  • signal transduction Source: SGD

Keywordsi

Biological processSugar transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29579-MONOMER

Protein family/group databases

TCDBi2.A.1.1.17 the major facilitator superfamily (mfs)

Names & Taxonomyi

Protein namesi
Recommended name:
High-affinity glucose transporter SNF3
Gene namesi
Name:SNF3
Ordered Locus Names:YDL194W
ORF Names:D1234
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL194W
SGDiS000002353 SNF3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 97CytoplasmicSequence analysisAdd BLAST97
Transmembranei98 – 118Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini119 – 143ExtracellularSequence analysisAdd BLAST25
Transmembranei144 – 164Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini165 – 178CytoplasmicSequence analysisAdd BLAST14
Transmembranei179 – 199Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini200 – 202ExtracellularSequence analysis3
Transmembranei203 – 223Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini224 – 229CytoplasmicSequence analysis6
Transmembranei230 – 250Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini251 – 264ExtracellularSequence analysisAdd BLAST14
Transmembranei265 – 285Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini286 – 358CytoplasmicSequence analysisAdd BLAST73
Transmembranei359 – 379Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini380 – 391ExtracellularSequence analysisAdd BLAST12
Transmembranei392 – 412Helical; Name=8Sequence analysisAdd BLAST21
Topological domaini413 – 419CytoplasmicSequence analysis7
Transmembranei420 – 440Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini441 – 451ExtracellularSequence analysisAdd BLAST11
Transmembranei452 – 472Helical; Name=10Sequence analysisAdd BLAST21
Topological domaini473 – 488CytoplasmicSequence analysisAdd BLAST16
Transmembranei489 – 509Helical; Name=11Sequence analysisAdd BLAST21
Topological domaini510 – 521ExtracellularSequence analysisAdd BLAST12
Transmembranei522 – 542Helical; Name=12Sequence analysisAdd BLAST21
Topological domaini543 – 884CytoplasmicSequence analysisAdd BLAST342

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi112G → D in SNF3-142. 1 Publication1
Mutagenesisi153G → R in SNF3-72. 1 Publication1
Mutagenesisi229R → K in SNF3-1; constitutively signaling glucose receptor. 1 Publication1
Mutagenesisi402V → I in SNF3-39. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000503901 – 884High-affinity glucose transporter SNF3Add BLAST884

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi383N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP10870
PRIDEiP10870

PTM databases

iPTMnetiP10870

Expressioni

Inductioni

Glucose-repressible.

Interactioni

Protein-protein interaction databases

BioGridi3185172 interactors.
IntActiP10870 2 interactors.
STRINGi4932.YDL194W

Structurei

3D structure databases

ProteinModelPortaliP10870
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The C-terminal cytoplasmic domain seems to be important for SNF3 regulatory function.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00910000144141
HOGENOMiHOG000202870
InParanoidiP10870
KOiK08139
OrthoDBiEOG092C1Q5W

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR020846 MFS_dom
IPR005828 MFS_sugar_transport-like
IPR036259 MFS_trans_sf
IPR037792 Rgt2/Snf3
IPR003663 Sugar/inositol_transpt
IPR005829 Sugar_transporter_CS
PANTHERiPTHR23500:SF343 PTHR23500:SF343, 1 hit
PfamiView protein in Pfam
PF00083 Sugar_tr, 1 hit
PRINTSiPR00171 SUGRTRNSPORT
SUPFAMiSSF103473 SSF103473, 2 hits
TIGRFAMsiTIGR00879 SP, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit
PS00216 SUGAR_TRANSPORT_1, 1 hit
PS00217 SUGAR_TRANSPORT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P10870-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPNSNSSSE TLRQEKQGFL DKALQRVKGI ALRRNNSNKD HTTDDTTGSI
60 70 80 90 100
RTPTSLQRQN SDRQSNMTSV FTDDISTIDD NSILFSEPPQ KQSMMMSICV
110 120 130 140 150
GVFVAVGGFL FGYDTGLINS ITSMNYVKSH VAPNHDSFTA QQMSILVSFL
160 170 180 190 200
SLGTFFGALT APFISDSYGR KPTIIFSTIF IFSIGNSLQV GAGGITLLIV
210 220 230 240 250
GRVISGIGIG AISAVVPLYQ AEATHKSLRG AIISTYQWAI TWGLLVSSAV
260 270 280 290 300
SQGTHARNDA SSYRIPIGLQ YVWSSFLAIG MFFLPESPRY YVLKDKLDEA
310 320 330 340 350
AKSLSFLRGV PVHDSGLLEE LVEIKATYDY EASFGSSNFI DCFISSKSRP
360 370 380 390 400
KQTLRMFTGI ALQAFQQFSG INFIFYYGVN FFNKTGVSNS YLVSFITYAV
410 420 430 440 450
NVVFNVPGLF FVEFFGRRKV LVVGGVIMTI ANFIVAIVGC SLKTVAAAKV
460 470 480 490 500
MIAFICLFIA AFSATWGGVV WVISAELYPL GVRSKCTAIC AAANWLVNFI
510 520 530 540 550
CALITPYIVD TGSHTSSLGA KIFFIWGSLN AMGVIVVYLT VYETKGLTLE
560 570 580 590 600
EIDELYIKSS TGVVSPKFNK DIRERALKFQ YDPLQRLEDG KNTFVAKRNN
610 620 630 640 650
FDDETPRNDF RNTISGEIDH SPNQKEVHSI PERVDIPTST EILESPNKSS
660 670 680 690 700
GMTVPVSPSL QDVPIPQTTE PAEIRTKYVD LGNGLGLNTY NRGPPSLSSD
710 720 730 740 750
SSEDYTEDEI GGPSSQGDQS NRSTMNDIND YMARLIHSTS TASNTTDKFS
760 770 780 790 800
GNQSTLRYHT ASSHSDTTEE DSNLMDLGNG LALNAYNRGP PSILMNSSDE
810 820 830 840 850
EANGGETSDN LNTAQDLAGM KERMAQFAQS YIDKRGGLEP ETQSNILSTS
860 870 880
LSVMADTNEH NNEILHSSEE NATNQPVNEN NDLK
Length:884
Mass (Da):96,719
Last modified:October 3, 2006 - v3
Checksum:i8E2A5A1A7739344F
GO

Sequence cautioni

The sequence CAA58253 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03246 Genomic DNA Translation: AAA35060.1
X83276 Genomic DNA Translation: CAA58253.1 Different initiation.
Z74242 Genomic DNA Translation: CAA98771.1
BK006938 Genomic DNA Translation: DAA11669.1
PIRiA31928
RefSeqiNP_010087.1, NM_001180254.1

Genome annotation databases

EnsemblFungiiYDL194W; YDL194W; YDL194W
GeneIDi851333
KEGGisce:YDL194W

Similar proteinsi

Entry informationi

Entry nameiSNF3_YEAST
AccessioniPrimary (citable) accession number: P10870
Secondary accession number(s): D6VRF9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 3, 2006
Last modified: April 25, 2018
This is version 162 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome