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Protein

Aspartokinase

Gene

HOM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 48100 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-aspartate = ADP + 4-phospho-L-aspartate.

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Aspartokinase (HOM3)
  2. Aspartate-semialdehyde dehydrogenase (HOM2)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-homoserine from L-aspartate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Aspartokinase (HOM3)
  2. Aspartate-semialdehyde dehydrogenase (HOM2)
  3. Homoserine dehydrogenase (HOM6)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homoserine from L-aspartate, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Pathwayi: L-threonine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-threonine from L-aspartate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Aspartokinase (HOM3)
  2. Aspartate-semialdehyde dehydrogenase (HOM2)
  3. Homoserine dehydrogenase (HOM6)
  4. Homoserine kinase (THR1)
  5. Threonine synthase (THR4)
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • aspartate kinase activity Source: SGD
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • homoserine biosynthetic process Source: SGD
  • lysine biosynthetic process via diaminopimelate Source: GO_Central
  • methionine biosynthetic process Source: SGD
  • threonine biosynthetic process Source: SGD

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Threonine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YER052C-MONOMER
BRENDAi2.7.2.4 984
SABIO-RKiP10869
UniPathwayiUPA00034; UER00015
UPA00050; UER00461
UPA00051; UER00462

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartokinase (EC:2.7.2.4)
Alternative name(s):
Aspartate kinase
Gene namesi
Name:HOM3
Ordered Locus Names:YER052C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER052C
SGDiS000000854 HOM3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000666901 – 527AspartokinaseAdd BLAST527

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei333PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP10869
PaxDbiP10869
PRIDEiP10869

PTM databases

iPTMnetiP10869

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
FPR1P200813EBI-2430,EBI-6961

Protein-protein interaction databases

BioGridi36791, 263 interactors
DIPiDIP-1319N
IntActiP10869, 11 interactors
MINTiP10869
STRINGi4932.YER052C

Structurei

3D structure databases

ProteinModelPortaliP10869
SMRiP10869
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini367 – 440ACT 1PROSITE-ProRule annotationAdd BLAST74
Domaini442 – 527ACT 2PROSITE-ProRule annotationAdd BLAST86

Sequence similaritiesi

Belongs to the aspartokinase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000293094
InParanoidiP10869
KOiK00928
OMAiINIMMIS
OrthoDBiEOG092C1PG8

Family and domain databases

Gene3Di3.40.1160.10, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR002912 ACT_dom
IPR001048 Asp/Glu/Uridylate_kinase
IPR001341 Asp_kinase
IPR018042 Aspartate_kinase_CS
IPR027795 CASTOR_ACT_dom
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF13840 ACT_7, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR00657 asp_kinases, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 1 hit
PS00324 ASPARTOKINASE, 1 hit

Sequencei

Sequence statusi: Complete.

P10869-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPMDFQPTSS HSNWVVQKFG GTSVGKFPVQ IVDDIVKHYS KPDGPNNNVA
60 70 80 90 100
VVCSARSSYT KAEGTTSRLL KCCDLASQES EFQDIIEVIR QDHIDNADRF
110 120 130 140 150
ILNPALQAKL VDDTNKELEL VKKYLNASKV LGEVSSRTVD LVMSCGEKLS
160 170 180 190 200
CLFMTALCND RGCKAKYVDL SHIVPSDFSA SALDNSFYTF LVQALKEKLA
210 220 230 240 250
PFVSAKERIV PVFTGFFGLV PTGLLNGVGR GYTDLCAALI AVAVNADELQ
260 270 280 290 300
VWKEVDGIFT ADPRKVPEAR LLDSVTPEEA SELTYYGSEV IHPFTMEQVI
310 320 330 340 350
RAKIPIRIKN VQNPLGNGTI IYPDNVAKKG ESTPPHPPEN LSSSFYEKRK
360 370 380 390 400
RGATAITTKN DIFVINIHSN KKTLSHGFLA QIFTILDKYK LVVDLISTSE
410 420 430 440 450
VHVSMALPIP DADSLKSLRQ AEEKLRILGS VDITKKLSIV SLVGKHMKQY
460 470 480 490 500
IGIAGTMFTT LAEEGINIEM ISQGANEINI SCVINESDSI KALQCIHAKL
510 520
LSERTNTSNQ FEHAIDERLE QLKRLGI
Length:527
Mass (Da):58,110
Last modified:August 1, 1990 - v2
Checksum:iD4D28FB8D4374898
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03526 Genomic DNA Translation: AAA34681.1
U18796 Genomic DNA Translation: AAB64587.1
BK006939 Genomic DNA Translation: DAA07708.1
PIRiA35888 KIBYD
RefSeqiNP_010972.1, NM_001178943.1

Genome annotation databases

EnsemblFungiiYER052C; YER052C; YER052C
GeneIDi856778
KEGGisce:YER052C

Similar proteinsi

Entry informationi

Entry nameiAK_YEAST
AccessioniPrimary (citable) accession number: P10869
Secondary accession number(s): D3DLV4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: August 1, 1990
Last modified: May 23, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

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