Reviewed,
UniProtKB/Swiss-Prot P10869 (AK_YEAST)
Last modified
February 9, 2010.
Version 93.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Aspartokinase EC=2.7.2.4 Alternative name(s): Aspartate kinase | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4932 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 527 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | ATP + L-aspartate = ADP + 4-phospho-L-aspartate. |
| Pathway | Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5. |
| Miscellaneous | Present with 48100 molecules/cell in log phase SD medium. Ref.4 |
| Sequence similarities | Belongs to the aspartokinase family. Contains 2 ACT domains. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Threonine biosynthesis |
| Domain | Repeat |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Transferase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | methionine metabolic process Traceable author statement. Source: SGD response to drugInferred from mutant phenotype. Source: SGD threonine biosynthetic processInferred from direct assay. Source: SGD |
| Cellular component | cytoplasm Inferred from direct assay. Source: SGD |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW amino acid bindingInferred from electronic annotation. Source: InterPro aspartate kinase activityInferred from direct assay. Source: SGD protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 527 | 527 | Aspartokinase | PRO_0000066690 | |||||
Regions | |||||||||
| Domain | 363 – 433 | 71 | ACT 1 | ||||||
| Domain | 438 – 506 | 69 | ACT 2 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 333 | 1 | Phosphothreonine Ref.5 Ref.6 Ref.7 | ||||||
| Modified residue | 441 | 1 | Phosphoserine Ref.7 | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Structure of the yeast HOM3 gene which encodes aspartokinase." Rafalski J.A., Falco S.C. J. Biol. Chem. 263:2146-2151(1988) [PubMed: 2892836] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Structure of the yeast HOM3 gene which encodes aspartokinase." Rafalski J.A., Falco S.C. J. Biol. Chem. 265:15346-15346(1990) [PubMed: 2168408] [Abstract] Cited for: SEQUENCE REVISION. |
| [3] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W.Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [4] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [5] | "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-333, MASS SPECTROMETRY. |
| [6] | "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-333, MASS SPECTROMETRY. |
| [7] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-333 AND SER-441, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | J03526 Genomic DNA. Translation: AAA34681.1. U18796 Genomic DNA. Translation: AAB64587.1. |
| PIR | KIBYD. A35888. |
| RefSeq | NP_010972.1. |
3D structure databases | |
| SMR | P10869. Positions 15-502. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-1319N. |
| IntAct | P10869. 14 interactions. |
| STRING | P10869. |
Proteomic databases | |
| PeptideAtlas | P10869. |
| PRIDE | P10869. |
Genome annotation databases | |
| Ensembl | YER052C; YER052C; YER052C; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 856778. |
| KEGG | sce:YER052C. |
| NMPDR | fig|4932.3.peg.2035. |
Organism-specific databases | |
| CYGD | YER052c. |
| SGD | S000000854. HOM3. |
Phylogenomic databases | |
| eggNOG | fuNOG04731. |
| HOGENOM | HBG724395. |
| OMA | FLAHIFT. |
| OrthoDB | EOG9W9KM6. |
| PhylomeDB | P10869. |
Enzyme and pathway databases | |
| BRENDA | 2.7.2.4. 250. |
Gene expression databases | |
| ArrayExpress | P10869. |
| Genevestigator | P10869. |
| GermOnline | YER052C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR002912. ACT_bd. IPR001048. Asp/Glu/Uridylate_kinase. IPR001341. Asp_kinase_dom. IPR018042. Aspartate_kinase_CS. [Graphical view] |
| Gene3D | G3DSA:3.40.1160.10. Aa_kinase. 1 hit. |
| Pfam | PF00696. AA_kinase. 1 hit. PF01842. ACT. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00657. asp_kinases. 1 hit. |
| PROSITE | PS00324. ASPARTOKINASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 982982. |
Entry information
| Entry name | AK_YEAST | ||||||||
| Accession | Primary (citable) accession number: P10869 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome V Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names |

Clusters with


