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P10869

- AK_YEAST

UniProt

P10869 - AK_YEAST

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Protein

Aspartokinase

Gene
HOM3, YER052C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate = ADP + 4-phospho-L-aspartate.

Pathwayi

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. aspartate kinase activity Source: SGD
  3. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. homoserine biosynthetic process Source: SGD
  2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway
  3. methionine biosynthetic process Source: SGD
  4. threonine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Threonine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YER052C-MONOMER.
BRENDAi2.7.2.4. 984.
SABIO-RKP10869.
UniPathwayiUPA00034; UER00015.
UPA00050; UER00461.
UPA00051; UER00462.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartokinase (EC:2.7.2.4)
Alternative name(s):
Aspartate kinase
Gene namesi
Name:HOM3
Ordered Locus Names:YER052C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER052c.
SGDiS000000854. HOM3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527AspartokinasePRO_0000066690Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei333 – 3331Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP10869.
PaxDbiP10869.
PeptideAtlasiP10869.

Expressioni

Gene expression databases

GenevestigatoriP10869.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
FPR1P200815EBI-2430,EBI-6961

Protein-protein interaction databases

BioGridi36791. 157 interactions.
DIPiDIP-1319N.
IntActiP10869. 8 interactions.
MINTiMINT-392511.
STRINGi4932.YER052C.

Structurei

3D structure databases

ProteinModelPortaliP10869.
SMRiP10869. Positions 16-501.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini367 – 44074ACT 1Add
BLAST
Domaini442 – 52786ACT 2Add
BLAST

Sequence similaritiesi

Belongs to the aspartokinase family.
Contains 2 ACT domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0527.
HOGENOMiHOG000293094.
KOiK00928.
OMAiQSCLQIK.
OrthoDBiEOG7NPG3J.

Family and domain databases

Gene3Di3.40.1160.10. 2 hits.
InterProiIPR002912. ACT_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001341. Asp_kinase_dom.
IPR018042. Aspartate_kinase_CS.
IPR027795. GATS-like_ACT_dom.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF13840. ACT_7. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR00657. asp_kinases. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS00324. ASPARTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10869-1 [UniParc]FASTAAdd to Basket

« Hide

MPMDFQPTSS HSNWVVQKFG GTSVGKFPVQ IVDDIVKHYS KPDGPNNNVA    50
VVCSARSSYT KAEGTTSRLL KCCDLASQES EFQDIIEVIR QDHIDNADRF 100
ILNPALQAKL VDDTNKELEL VKKYLNASKV LGEVSSRTVD LVMSCGEKLS 150
CLFMTALCND RGCKAKYVDL SHIVPSDFSA SALDNSFYTF LVQALKEKLA 200
PFVSAKERIV PVFTGFFGLV PTGLLNGVGR GYTDLCAALI AVAVNADELQ 250
VWKEVDGIFT ADPRKVPEAR LLDSVTPEEA SELTYYGSEV IHPFTMEQVI 300
RAKIPIRIKN VQNPLGNGTI IYPDNVAKKG ESTPPHPPEN LSSSFYEKRK 350
RGATAITTKN DIFVINIHSN KKTLSHGFLA QIFTILDKYK LVVDLISTSE 400
VHVSMALPIP DADSLKSLRQ AEEKLRILGS VDITKKLSIV SLVGKHMKQY 450
IGIAGTMFTT LAEEGINIEM ISQGANEINI SCVINESDSI KALQCIHAKL 500
LSERTNTSNQ FEHAIDERLE QLKRLGI 527
Length:527
Mass (Da):58,110
Last modified:August 1, 1990 - v2
Checksum:iD4D28FB8D4374898
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03526 Genomic DNA. Translation: AAA34681.1.
U18796 Genomic DNA. Translation: AAB64587.1.
BK006939 Genomic DNA. Translation: DAA07708.1.
PIRiA35888. KIBYD.
RefSeqiNP_010972.1. NM_001178943.1.

Genome annotation databases

EnsemblFungiiYER052C; YER052C; YER052C.
GeneIDi856778.
KEGGisce:YER052C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03526 Genomic DNA. Translation: AAA34681.1 .
U18796 Genomic DNA. Translation: AAB64587.1 .
BK006939 Genomic DNA. Translation: DAA07708.1 .
PIRi A35888. KIBYD.
RefSeqi NP_010972.1. NM_001178943.1.

3D structure databases

ProteinModelPortali P10869.
SMRi P10869. Positions 16-501.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36791. 157 interactions.
DIPi DIP-1319N.
IntActi P10869. 8 interactions.
MINTi MINT-392511.
STRINGi 4932.YER052C.

Proteomic databases

MaxQBi P10869.
PaxDbi P10869.
PeptideAtlasi P10869.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER052C ; YER052C ; YER052C .
GeneIDi 856778.
KEGGi sce:YER052C.

Organism-specific databases

CYGDi YER052c.
SGDi S000000854. HOM3.

Phylogenomic databases

eggNOGi COG0527.
HOGENOMi HOG000293094.
KOi K00928.
OMAi QSCLQIK.
OrthoDBi EOG7NPG3J.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00015 .
UPA00050 ; UER00461 .
UPA00051 ; UER00462 .
BioCyci YEAST:YER052C-MONOMER.
BRENDAi 2.7.2.4. 984.
SABIO-RK P10869.

Miscellaneous databases

NextBioi 982982.
PROi P10869.

Gene expression databases

Genevestigatori P10869.

Family and domain databases

Gene3Di 3.40.1160.10. 2 hits.
InterProi IPR002912. ACT_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001341. Asp_kinase_dom.
IPR018042. Aspartate_kinase_CS.
IPR027795. GATS-like_ACT_dom.
[Graphical view ]
Pfami PF00696. AA_kinase. 1 hit.
PF13840. ACT_7. 1 hit.
[Graphical view ]
SUPFAMi SSF53633. SSF53633. 1 hit.
TIGRFAMsi TIGR00657. asp_kinases. 1 hit.
PROSITEi PS51671. ACT. 1 hit.
PS00324. ASPARTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of the yeast HOM3 gene which encodes aspartokinase."
    Rafalski J.A., Falco S.C.
    J. Biol. Chem. 263:2146-2151(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Structure of the yeast HOM3 gene which encodes aspartokinase."
    Rafalski J.A., Falco S.C.
    J. Biol. Chem. 265:15346-15346(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-333, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAK_YEAST
AccessioniPrimary (citable) accession number: P10869
Secondary accession number(s): D3DLV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: August 1, 1990
Last modified: May 14, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 48100 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

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