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Protein

L-gulonolactone oxidase

Gene

Gulo

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Oxidizes L-gulono-1,4-lactone to hydrogen peroxide and L-xylo-hexulonolactone which spontaneously isomerizes to L-ascorbate.1 Publication

Catalytic activityi

L-gulono-1,4-lactone + O2 = L-ascorbate + H2O2.

Cofactori

Pathwayi: L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway

This protein is involved in step 4 of the subpathway that synthesizes L-ascorbate from UDP-alpha-D-glucuronate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Regucalcin (Rgn)
  4. L-gulonolactone oxidase (Gulo)
This subpathway is part of the pathway L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ascorbate from UDP-alpha-D-glucuronate, the pathway L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAscorbate biosynthesis
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13235
UniPathwayiUPA00991; UER00939

Names & Taxonomyi

Protein namesi
Recommended name:
L-gulonolactone oxidase (EC:1.1.3.8)
Short name:
LGO
Alternative name(s):
L-gulono-gamma-lactone oxidase
Short name:
GLO
Gene namesi
Name:Gulo
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620701 Gulo

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei251 – 273HelicalSequence analysisAdd BLAST23

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001281612 – 440L-gulonolactone oxidaseAdd BLAST439

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54Pros-8alpha-FAD histidineBy similarity1

Proteomic databases

PaxDbiP10867
PRIDEiP10867

PTM databases

iPTMnetiP10867
PhosphoSitePlusiP10867

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022703

Structurei

3D structure databases

SMRiP10867
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 187FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST171

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4730 Eukaryota
COG0277 LUCA
HOGENOMiHOG000252847
HOVERGENiHBG005834
InParanoidiP10867
KOiK00103
PhylomeDBiP10867

Family and domain databases

Gene3Di3.30.43.10, 1 hit
InterProiView protein in InterPro
IPR007173 ALO
IPR016166 FAD-bd_2
IPR036318 FAD-bd_2-like_sf
IPR016167 FAD-bd_2_sub1
IPR010031 FAD_lactone_oxidase
IPR006094 Oxid_FAD_bind_N
IPR006093 Oxy_OxRdtase_FAD_BS
IPR030654 Sugar_lactone_oxidase
PANTHERiPTHR43762 PTHR43762, 1 hit
PfamiView protein in Pfam
PF04030 ALO, 1 hit
PF01565 FAD_binding_4, 1 hit
PIRSFiPIRSF000136 LGO_GLO, 1 hit
SUPFAMiSSF56176 SSF56176, 1 hit
TIGRFAMsiTIGR01678 FAD_lactone_ox, 1 hit
PROSITEiView protein in PROSITE
PS51387 FAD_PCMH, 1 hit
PS00862 OX2_COVAL_FAD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10867-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHGYKGVQF QNWAKTYGCS PEVYYQPTSV EEVREVLALA REQKKKVKVV
60 70 80 90 100
GGGHSPSDIA CTDGFMIHMG KMNRVLQVDK EKKQITVEAG ILLADLHPQL
110 120 130 140 150
DEHGLAMSNL GAVSDVTVAG VIGSGTHNTG IKHGILATQV VALTLMTADG
160 170 180 190 200
EVLECSESRN ADVFQAARVH LGCLGIILTV TLQCVPQFHL QETSFPSTLK
210 220 230 240 250
EVLDNLDSHL KRSEYFRFLW FPHTENVSII YQDHTNKAPS SASNWFWDYA
260 270 280 290 300
IGFYLLEFLL WTSTYLPCLV GWINRFFFWM LFNCKKESSN LSHKIFTYEC
310 320 330 340 350
RFKQHVQDWA IPREKTKEAL LELKAMLEAH PKVVAHYPVE VRFTRGDDIL
360 370 380 390 400
LSPCFQRDSC YMNIIMYRPY GKDVPRLDYW LAYETIMKKF GGRPHWAKAH
410 420 430 440
NCTQKDFEEM YPTFHKFCDI REKLDPTGMF LNSYLEKVFY
Length:440
Mass (Da):50,615
Last modified:January 23, 2007 - v3
Checksum:iD63AD580A6F591F8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85I → V in AAH89803 (PubMed:15489334).Curated1
Sequence conflicti189H → Q in AAA41164 (PubMed:3338984).Curated1
Sequence conflicti404Q → R (PubMed:1400507).Curated1
Sequence conflicti404Q → R (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03536 Genomic DNA Translation: AAA41164.1
D12754 Genomic DNA Translation: BAA02232.1
D00526 Genomic DNA Translation: BAA33497.1
BC089803 mRNA Translation: AAH89803.1
PIRiA45123 OXRTGU
RefSeqiNP_071556.2, NM_022220.2
UniGeneiRn.115212

Genome annotation databases

GeneIDi60671
KEGGirno:60671
UCSCiRGD:620701 rat

Similar proteinsi

Entry informationi

Entry nameiGGLO_RAT
AccessioniPrimary (citable) accession number: P10867
Secondary accession number(s): Q5EBC1, Q64597, Q9QWR6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 153 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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