P10866 (HN_MUMPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hemagglutinin-neuraminidase
EC=3.2.1.18

Gene names

Name:

HN

Organism

Mumps virus (strain RW) (MuV)

Taxonomic identifier

11172 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Rubulavirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	582 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity. Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins By similarity.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subcellular location	Virion membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.

Ontologies

Keywords
Biological process	Host-virus interaction Initiation of viral infection Viral attachment to host cell
Cellular component	Host cell membrane Host membrane Membrane Viral envelope protein Virion
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hemagglutinin Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: InterPro virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	exo-alpha-(2->3)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->6)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->8)-sialidase activity Inferred from electronic annotation. Source: EC host cell surface receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 582	582	Hemagglutinin-neuraminidase		PRO_0000142604
Regions
	Topological domain	1 – 34	34	Cytoplasmic Potential
	Transmembrane	35 – 55	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	56 – 582	527	Extracellular Potential
Amino acid modifications
	Glycosylation	127	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	284	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	329	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	400	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	448	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	507	1	N-linked (GlcNAc...); by host Potential

Sequences

	Sequence		Length	Mass (Da)
	P10866 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 6BCCB6990E65F869	FASTA	582	64,045
	10 20 30 40 50 60 MEPSKLFTIS DNATFAPGPV NNAADKKTFR TCFRILVLSV QAVTLILVIV TLGELVRMIN 70 80 90 100 110 120 DQGLSNQLSS ITDKIRESAT MIASAVGVMN QVIHGVTVSL PLQIEGNQNQ LLSTLATICT 130 140 150 160 170 180 SKKQISNCST NIPLVNDLRF INGINKFIIE DYANHDFSIG HPLNMPSFIP TATSPNGCTR 190 200 210 220 230 240 IPSFSLGKTH WCYTHNVINA NCKDHTSSNQ YVSMGILVQT ASGYPMFKTL KIQYLSDGLN 250 260 270 280 290 300 RKSCSIATVP DGCAMYCYVS TQLETDDYAG SSPPTQKLTL LFYNDTVTER TISPSGLEGN 310 320 330 340 350 360 WATLVPGVGS GIYFENKLIF PAYGGVLPNS TLGVKLAREF FRPVNPYNPC SGPQQDLDQR 370 380 390 400 410 420 ALRSYFPSYL SNRRVQSAFL VCAWNQILVT NCELVVPSNN QTLMGAEGRV LLINNRLLYY 430 440 450 460 470 480 QRSTSWWPYE LLYEISFTFT NSGQSSVNMS WIPIYSFTRP GSGKCSGENV CPIACVSGVY 490 500 510 520 530 540 LDPWPLTPYS HQSGINRNFY FTGALLNSST TRVNPTLYVS ALNNLKVLAP YGTQGLSASY 550 560 570 580 TTTTCFQDTG DASVYCVYIM ELASNIVGEF QILPVLTRLT IT

References

[1]	"Sequence determination of the mumps virus HN gene." Waxham M.N., Aronowski J., Server A.C., Wolinsky J.S., Smith J.A., Goodman H.M. Virology 164:318-325(1988) [PubMed: 3369084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M19933 mRNA. Translation: AAA46609.1.
PIR	HNNZMP. A28917.
3D structure databases
ProteinModelPortal	P10866.
ModBase	Search...
Protein family/group databases
CAZy	GH83. Glycoside Hydrolase Family 83.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000665. Hemagglutn-neuramid_glycoprot. IPR016285. Hemagglutn-neuramid_paramyxo. IPR011040. Neuraminidase. [Graphical view]
Gene3D	G3DSA:2.120.10.10. Neuraminidase. 1 hit.
Pfam	PF00423. HN. 1 hit. [Graphical view]
PIRSF	PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAM	SSF50939. Sialidase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HN_MUMPR

Accession

Primary (citable) accession number: P10866

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program