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Protein

Postreplication repair E3 ubiquitin-protein ligase RAD18

Gene

RAD18

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 RING-finger protein, member of the UBC2/RAD6 epistasis group. Associates to the E2 ubiquitin conjugating enzyme UBC2/RAD6 to form the UBC2-RAD18 ubiquitin ligase complex involved in postreplicative repair (PRR) of damaged DNA. The UBC2-RAD18 complex cooperates with RAD5 and the UBC13-MMS2 dimer to attach mono-ubiquitin chains on 'Lys-164' of POL30, which is necessary for PRR. The UBC2-RAD18 complex is also involved in prevention of spontaneous mutations caused by 7,8-dihydro-8-oxoguanine.8 Publications

Miscellaneous

Present with 206 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri28 – 66RING-typePROSITE-ProRule annotationAdd BLAST39
Zinc fingeri190 – 210UBZ-typeAdd BLAST21

GO - Molecular functioni

GO - Biological processi

  • error-free postreplication DNA repair Source: SGD
  • error-free translesion synthesis Source: SGD
  • error-prone translesion synthesis Source: SGD
  • protein monoubiquitination Source: SGD
  • response to UV Source: GO_Central

Keywordsi

Molecular functionDNA-binding, Transferase
Biological processDNA damage, DNA repair, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29369-MONOMER
ReactomeiR-SCE-110314 Recognition of DNA damage by PCNA-containing replication complex
R-SCE-8866654 E3 ubiquitin ligases ubiquitinate target proteins
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Postreplication repair E3 ubiquitin-protein ligase RAD18 (EC:2.3.2.27)
Alternative name(s):
RING-type E3 ubiquitin transferase RAD18Curated
Radiation sensitivity protein 18
Gene namesi
Name:RAD18
Ordered Locus Names:YCR066W
ORF Names:YCR66W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR066W
SGDiS000000662 RAD18

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000561611 – 487Postreplication repair E3 ubiquitin-protein ligase RAD18Add BLAST487

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei155PhosphothreonineCombined sources1
Modified residuei174PhosphoserineCombined sources1
Cross-linki204Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP10862
PaxDbiP10862
PRIDEiP10862

PTM databases

iPTMnetiP10862

Interactioni

Subunit structurei

Homodimer. Interacts with E2 UBC2, forming a complex with ubiquitin ligase activity. The UBC2-RAD18 complex interacts itself with the UBC13-MMS2 ubiquitin ligase complex through direct interactions of both RAD18 and UBC13 with RAD5. Interacts also with UBC9. Binds single strand DNA.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RAD6P061044EBI-14659,EBI-19722

Protein-protein interaction databases

BioGridi31042, 226 interactors
DIPiDIP-1172N
IntActiP10862, 12 interactors
MINTiP10862
STRINGi4932.YCR066W

Structurei

3D structure databases

ProteinModelPortaliP10862
SMRiP10862
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini278 – 312SAPPROSITE-ProRule annotationAdd BLAST35

Sequence similaritiesi

Belongs to the RAD18 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri28 – 66RING-typePROSITE-ProRule annotationAdd BLAST39
Zinc fingeri190 – 210UBZ-typeAdd BLAST21

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000011230
HOGENOMiHOG000248313
InParanoidiP10862
KOiK10627
OMAiNFLIREM
OrthoDBiEOG092C5X0S

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR004580 Rad18
IPR003034 SAP_dom
IPR006642 Znf_Rad18_put
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF02037 SAP, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SM00513 SAP, 1 hit
SM00734 ZnF_Rad18, 1 hit
TIGRFAMsiTIGR00599 rad18, 1 hit
PROSITEiView protein in PROSITE
PS50800 SAP, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

P10862-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDHQITTASD FTTTSIPSLY QLDTLLRCHI CKDFLKVPVL TPCGHTFCSL
60 70 80 90 100
CIRTHLNNQP NCPLCLFEFR ESLLRSEFLV SEIIQSYTSL RSSLLDALRI
110 120 130 140 150
PKPTPVPENE EVPGPENSSW IELISESESD SVNAADDDLQ IVATSERKLA
160 170 180 190 200
KRSMTDILPL SSKPSKRNFA MFRSERIKKK SKPNEQMAQC PICQQFYPLK
210 220 230 240 250
ALEKTHLDEC LTLQSLGKKP KISTTFPTES NPHNKSSSRF KVRTPEVDKS
260 270 280 290 300
SCGETSHVDK YLNSMMSAEH QRLPKINFTS MTQSQIKQKL SSLGLSTNGT
310 320 330 340 350
RQNMIKRYNH YEMLWNSNFC DSLEPVDEAE LKRQLLSWDV SHNKTPQNSS
360 370 380 390 400
NKGGISKLMI MKSNGKSSSY RKLLENFKND KFNRKGWMVM FRKDFARLIR
410 420 430 440 450
EAKMKIKTGS SDSSGSVGHS NDGDGVEKVQ SDQGTEDQQM EKDQDTVINE
460 470 480
DRVAGERNLP NEDSTDADLS RELMDLNEYS KDPPGNN
Length:487
Mass (Da):55,230
Last modified:July 1, 1989 - v1
Checksum:i70F5F12A3FA99532
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12542 Genomic DNA Translation: CAA31059.1
M36405 Genomic DNA Translation: AAA34932.1
X12588 Genomic DNA Translation: CAA31101.1
X59720 Genomic DNA Translation: CAA42281.1
BK006937 Genomic DNA Translation: DAA07538.1
PIRiS05802 DDBY18
RefSeqiNP_009992.1, NM_001178777.1

Genome annotation databases

EnsemblFungiiCAA42281; CAA42281; CAA42281
YCR066W; YCR066W; YCR066W
GeneIDi850430
KEGGisce:YCR066W

Similar proteinsi

Entry informationi

Entry nameiRAD18_YEAST
AccessioniPrimary (citable) accession number: P10862
Secondary accession number(s): D6VR69, Q58AT6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 23, 2018
This is version 185 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

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