Glutamate dehydrogenase 1, mitochondrial precursor

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Reviewed, UniProtKB/Swiss-Prot P10860 (DHE3_RAT)

Last modified November 25, 2008. Version 87. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Glutamate dehydrogenase 1, mitochondrial
      Short name=GDH
    EC=1.4.1.3
Alternative name(s):
    Memory-related protein 2
    MRG-2

Gene names

Name:	Glud1
Synonyms:	Glud

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
Catalytic activity	L-glutamate + H(2)O + NAD(P)(+) = 2-oxoglutarate + NH(3) + NAD(P)H.
Enzyme regulation	Subject to allosteric regulation. Activated by ADP. Inhibited by GTP and ATP. ADP can occupy the NADH binding site and activate the enzyme By similarity.
Subunit structure	Homohexamer.
Subcellular location	Mitochondrion matrix.
Tissue specificity	Widely expressed throughout the hippocampus. After induction by training, highly expressed in the dentate gyrus, pyrimidal layer and lacunosum molecolare.
Induction	By water maze training in the hippocampus and in other regions of the brain including the laterodorsal nucleus of the thalamus and the cingulate cortex.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

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Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding GTP-binding NADP Nucleotide-binding
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 53

Mitochondrion By similarity

Chain

54 – 558

505

Glutamate dehydrogenase 1, mitochondrial

PRO_0000007213

Regions

Nucleotide binding

141 – 143

NAD By similarity

Sites

Active site

183

Binding site

147

Substrate By similarity

Binding site

171

Substrate By similarity

Binding site

176

NAD By similarity

Binding site

252

NAD By similarity

Binding site

266

GTP By similarity

Binding site

270

GTP By similarity

Binding site

319

GTP By similarity

Binding site

322

GTP By similarity

Binding site

438

Substrate By similarity

Binding site

444

NAD By similarity

Binding site

450

ADP By similarity

Binding site

516

ADP By similarity

Amino acid modifications

Modified residue

N6-acetyllysine By similarity

Modified residue

135

Phosphotyrosine By similarity

Modified residue

227

Phosphoserine By similarity

Modified residue

503

N6-acetyllysine By similarity

Modified residue

512

Phosphotyrosine By similarity

Modified residue

527

N6-acetyllysine By similarity

Experimental info

Sequence conflict

56 – 57

AA → GP in CAA32441. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P10860-1 [UniParc].

Last modified April 4, 2006. Version 2.
Checksum: 388B8B5490C10F31
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FASTA

558

61,416

        10         20         30         40         50         60 
MYRRLGEVLL LSRAGPAALG SAAADSAALL GWARGQPSAV PQPGLTPVAR RHYSEAATDR 

        70         80         90        100        110        120 
EDDPNFFKMV EGFFDRGASI VEDKLVEDLK TRENEEQKRN RVRGILRIIK PCNHVLSLSF 

       130        140        150        160        170        180 
PIRRDDGSWE VIEGYRAQHS QHRTPCKGGI RYSTDVSVDE VKALASLMTY KCAVVDVPFG 

       190        200        210        220        230        240 
GAKAGVKINP KNYTDNELEK ITRRFTMELA KKGFIGPGID VPAPDMSTGE REMSWIADTY 

       250        260        270        280        290        300 
ASTIGHYDIN AHACVTGKPI SQGGIHGRIS ATGRGVFHGI ENFINEASYM SILGMTPGLG 

       310        320        330        340        350        360 
DKTFVVQGFG NVGLHSMRYL HRFGAKCVGV GESDGSIWNP DGIDPKELED FKLQHGSILG 

       370        380        390        400        410        420 
FPKAKVYEGS ILEADCDILI PAASEKQLTK SNAPRVKAKI IAEGANGPTT PEADKIFLER 

       430        440        450        460        470        480 
NIMVIPDLYL NAGGVTVSYF EWLKNLNHVS YGRLTFKYER DSNYHLLMSV QESLERKFGK 

       490        500        510        520        530        540 
HGGTIPVVPT AEFQDRISGA SEKDIVHSGL AYTMERSARQ IMRTAMKYNL GLDLRTAAYV 

       550 
NAIEKVFKVY NEAGVTFT

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of rat liver glutamate dehydrogenase cDNA." Amuro N., Ooki K., Ito A., Goto Y., Okazaki T. Nucleic Acids Res. 17:2356-2357(1989) [PubMed: 2704625] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"Nucleotide sequence of rat liver glutamate dehydrogenase cDNA." Das A.T., Moerer P., Lamers W.H. Nucleic Acids Res. 17:2355-2355(1989) [PubMed: 2704624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[4]	"Isolation and characterization of the rat gene encoding glutamate dehydrogenase." Das A.T., Arnberg A.C., Malingre H., Moerer P., Charles R., Moorman A.F.M., Lamers W.H. Eur. J. Biochem. 211:795-803(1993) [PubMed: 8094669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30. Strain: Wistar. Tissue: Liver.
[5]	Lubec G., Afjehi-Sadat L., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 108-123; 303-318; 347-363; 400-420; 481-496 AND 504-516, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus and Spinal cord.
[6]	"Identification of the CoA-modified forms of mitochondrial acetyl-CoA acetyltransferase and of glutamate dehydrogenase as nearest-neighbour proteins." Schwerdt G., Moller U., Huth W. Biochem. J. 280:353-357(1991) [PubMed: 1684101] [Abstract] Cited for: PROTEIN SEQUENCE OF 161-190.
[7]	"Late memory-related genes in the hippocampus revealed by RNA fingerprinting." Cavallaro S., Meiri N., Yi C.-L., Musco S., Ma W., Goldberg J., Alkon D.L. Proc. Natl. Acad. Sci. U.S.A. 94:9669-9673(1997) [PubMed: 9275181] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 431-558, FUNCTION, TISSUE SPECIFICITY, INDUCTION. Strain: Wistar. Tissue: Hippocampus.

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Cross-references

Sequence databases
	X14223 mRNA. Translation: CAA32441.1. X14044 mRNA. Translation: CAA32202.1. BC081841 mRNA. Translation: AAH81841.1. X64365 Genomic DNA. Translation: CAA45717.1. U95148 mRNA. Translation: AAB70012.1.
PIR	S03707.
RefSeq	NP_036702.1.
UniGene	Rn.55106
3D structure databases
HSSP	HSSP built from PDB template 1L1F based on UniProtKB P00367.
SMR	P10860. Positions 59-558.
ModBase	Search...
PTM databases
PhosphoSite	P10860.
Genome annotation databases
Ensembl	ENSRNOG00000010222. Rattus norvegicus. [Contig view]
GeneID	24399.
KEGG	rno:24399.
NMPDR	fig\|10116.3.peg.12078.
Organism-specific databases
RGD	2708. Glud1.
Phylogenomic databases
HOVERGEN	P10860.
Gene expression databases
ArrayExpress	P10860.
GermOnline	ENSRNOG00000010222. Rattus norvegicus.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DHase. IPR006096. Glu/Leu/Phe/Val_DHase_C. IPR006097. Glu/Leu/Phe/Val_DHase_dimer. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	603195.

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Entry information

Entry name

DHE3_RAT

Accession

Primary (citable) accession number: P10860
Secondary accession number(s): Q66HI8, Q6LC16

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	April 4, 2006
Last modified:	November 25, 2008
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents