SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P10857

- SYLC_NEUCR

UniProt

P10857 - SYLC_NEUCR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Leucine--tRNA ligase, cytoplasmic
Gene
leu-6, B10K17.030, NCU09463
Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei760 – 7601ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. leucine-tRNA ligase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA replication initiation Source: EnsemblFungi
  2. leucyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase, cytoplasmic (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:leu-6
ORF Names:B10K17.030, NCU09463
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 6, Linkage Group II

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11231123Leucine--tRNA ligase, cytoplasmic
PRO_0000152153Add
BLAST

Proteomic databases

PRIDEiP10857.

Interactioni

Protein-protein interaction databases

STRINGi5141.NCU09463.1.

Structurei

3D structure databases

ProteinModelPortaliP10857.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi84 – 9411"HIGH" region
Add
BLAST
Motifi757 – 7615"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216621.
KOiK01869.
OMAiHAPFDYL.
OrthoDBiEOG7TTQH2.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10857-1 [UniParc]FASTAAdd to Basket

« Hide

MADTAAVAKG VENLSVSASK TKELKGTEKR DTLIEIEKRY QQKWEQEGVF     50
EVDAPSTAEF PLDAITPDEL RQKHPKFFGT IAYPYMNGRL HAGHAFSFSK 100
IEYHTGFARM QGKRALFPQG YHCTGLPIKA SADKLVKEIE MFGQEFERYK 150
EDEVVEGAAP AAAAAPKTKE DLTKFNAKKG KTVAKTGGAK YQFQILKSLG 200
IPVSEIHKFA DPQYWLHYFP PECKKDLTNF GARIDWRRQF VTTDANPYYD 250
AFVRWQMNRL LELNKIKFGK RYTIYSIKDG QPCMDHDRSE GEGVLPQEYT 300
ALKLKVTEWA PKAAEALKGK LPEGANVYLC PATLRPETMY GQVCCFVGPA 350
LKYGVFKAAE NEYFVITERA AKNMAYQGIF EKEGVIEKAA DIVGSDLIGT 400
LVNAPLSVHK EVYVLPMDTV LATKGTGVVT SVPSDSPDDC AMMTELAKKP 450
EFYGIQKEWA EKEIVSVIKT PTSDLLAPYL VKKLKINSPK DAKQLLEAKE 500
LAYKEGFYQG IMNYGDFKGE KVETAKPKVR QQLIDAGDAF AYSEPENKVV 550
SRSGDECSVA LMDQWYIDYG EDSWRTVLYD YVENKDGKGI NTYYADTQHA 600
FKGVIGWLKQ WACARTYGLG SKLPWDPNFL VESLSDSTVY MAYYTVAHWL 650
HRDLFGREKG KGNIGADQMI DEVWDYIFCR TELSDHLVTK SGIPKETLDS 700
MRREFQYFYP LDIRVSGKDL IPNHLTFWLY NHIALFPREY WPKSVRANGH 750
LQLNGEKMSK STGNFMTLDD VVKKYGADAA RVALADAGDG ISDSNFVEDV 800
ADNTILRFYT NKEWIEETLK DESLRTGELN SFQDALFDNE MNALVNEARK 850
HYEETSYKLA LKAAHYDFLN ARDMYREACA AAGIPLHKDL VTKYIRLQAL 900
VITPIAPHWA DYVWQECLGE PKSIQFARWP EVPAANPALT AARDYVRTTS 950
SAINSAEAAQ LKKMAKGRQS DFDPKKPKKL TIFATENFPT WQAKYIDLLS 1000
EVWDAATGTQ KIDDKELNGR IAKMGEMKKA MPFVQALKKR LKDGEPAEQI 1050
LSRKLSFDEK ATLLAMIPGL KRTAGLESVQ VVLVEEGSKT GKDLTNGGAE 1100
IEVTAPMAEA ALPGQPSFFF TNV 1123
Length:1,123
Mass (Da):126,399
Last modified:April 1, 1990 - v2
Checksum:i78945AFC3D06FFBF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M30473 Genomic DNA. Translation: AAA33593.1.
BX842596 Genomic DNA. Translation: CAE75711.1.
CM002237 Genomic DNA. Translation: EAA33982.1.
X13021 Genomic DNA. Translation: CAA31439.1.
PIRiA33475. SYNCLC.
RefSeqiXP_963218.1. XM_958125.2.
UniGeneiNcr.15478.

Genome annotation databases

EnsemblFungiiEFNCRT00000009314; EFNCRP00000009296; EFNCRG00000009295.
GeneIDi3879366.
KEGGincr:NCU09463.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M30473 Genomic DNA. Translation: AAA33593.1 .
BX842596 Genomic DNA. Translation: CAE75711.1 .
CM002237 Genomic DNA. Translation: EAA33982.1 .
X13021 Genomic DNA. Translation: CAA31439.1 .
PIRi A33475. SYNCLC.
RefSeqi XP_963218.1. XM_958125.2.
UniGenei Ncr.15478.

3D structure databases

ProteinModelPortali P10857.
ModBasei Search...

Protein-protein interaction databases

STRINGi 5141.NCU09463.1.

Proteomic databases

PRIDEi P10857.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000009314 ; EFNCRP00000009296 ; EFNCRG00000009295 .
GeneIDi 3879366.
KEGGi ncr:NCU09463.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000216621.
KOi K01869.
OMAi HAPFDYL.
OrthoDBi EOG7TTQH2.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view ]
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsi TIGR00395. leuS_arch. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Regulation of the nuclear genes encoding the cytoplasmic and mitochondrial leucyl-tRNA synthetases of Neurospora crassa."
    Chow C.M., RajBhandary U.L.
    Mol. Cell. Biol. 9:4645-4652(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
    Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
    Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  3. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  4. "Cytoplasmic leucyl-tRNA synthetase of Neurospora crassa is not specified by the leu-5 locus."
    Benarous R., Chow C.M., RajBhandary U.L.
    Genetics 119:805-814(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-116.

Entry informationi

Entry nameiSYLC_NEUCR
AccessioniPrimary (citable) accession number: P10857
Secondary accession number(s): Q7RVI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: April 1, 1990
Last modified: May 14, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi