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P10857 (SYLC_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase, cytoplasmic

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leu-6
ORF Names:B10K17.030, NCU09463
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length1123 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication initiation

Inferred from electronic annotation. Source: EnsemblFungi

leucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11231123Leucine--tRNA ligase, cytoplasmic
PRO_0000152153

Regions

Motif84 – 9411"HIGH" region
Motif757 – 7615"KMSKS" region

Sites

Binding site7601ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P10857 [UniParc].

Last modified April 1, 1990. Version 2.
Checksum: 78945AFC3D06FFBF

FASTA1,123126,399
        10         20         30         40         50         60 
MADTAAVAKG VENLSVSASK TKELKGTEKR DTLIEIEKRY QQKWEQEGVF EVDAPSTAEF 

        70         80         90        100        110        120 
PLDAITPDEL RQKHPKFFGT IAYPYMNGRL HAGHAFSFSK IEYHTGFARM QGKRALFPQG 

       130        140        150        160        170        180 
YHCTGLPIKA SADKLVKEIE MFGQEFERYK EDEVVEGAAP AAAAAPKTKE DLTKFNAKKG 

       190        200        210        220        230        240 
KTVAKTGGAK YQFQILKSLG IPVSEIHKFA DPQYWLHYFP PECKKDLTNF GARIDWRRQF 

       250        260        270        280        290        300 
VTTDANPYYD AFVRWQMNRL LELNKIKFGK RYTIYSIKDG QPCMDHDRSE GEGVLPQEYT 

       310        320        330        340        350        360 
ALKLKVTEWA PKAAEALKGK LPEGANVYLC PATLRPETMY GQVCCFVGPA LKYGVFKAAE 

       370        380        390        400        410        420 
NEYFVITERA AKNMAYQGIF EKEGVIEKAA DIVGSDLIGT LVNAPLSVHK EVYVLPMDTV 

       430        440        450        460        470        480 
LATKGTGVVT SVPSDSPDDC AMMTELAKKP EFYGIQKEWA EKEIVSVIKT PTSDLLAPYL 

       490        500        510        520        530        540 
VKKLKINSPK DAKQLLEAKE LAYKEGFYQG IMNYGDFKGE KVETAKPKVR QQLIDAGDAF 

       550        560        570        580        590        600 
AYSEPENKVV SRSGDECSVA LMDQWYIDYG EDSWRTVLYD YVENKDGKGI NTYYADTQHA 

       610        620        630        640        650        660 
FKGVIGWLKQ WACARTYGLG SKLPWDPNFL VESLSDSTVY MAYYTVAHWL HRDLFGREKG 

       670        680        690        700        710        720 
KGNIGADQMI DEVWDYIFCR TELSDHLVTK SGIPKETLDS MRREFQYFYP LDIRVSGKDL 

       730        740        750        760        770        780 
IPNHLTFWLY NHIALFPREY WPKSVRANGH LQLNGEKMSK STGNFMTLDD VVKKYGADAA 

       790        800        810        820        830        840 
RVALADAGDG ISDSNFVEDV ADNTILRFYT NKEWIEETLK DESLRTGELN SFQDALFDNE 

       850        860        870        880        890        900 
MNALVNEARK HYEETSYKLA LKAAHYDFLN ARDMYREACA AAGIPLHKDL VTKYIRLQAL 

       910        920        930        940        950        960 
VITPIAPHWA DYVWQECLGE PKSIQFARWP EVPAANPALT AARDYVRTTS SAINSAEAAQ 

       970        980        990       1000       1010       1020 
LKKMAKGRQS DFDPKKPKKL TIFATENFPT WQAKYIDLLS EVWDAATGTQ KIDDKELNGR 

      1030       1040       1050       1060       1070       1080 
IAKMGEMKKA MPFVQALKKR LKDGEPAEQI LSRKLSFDEK ATLLAMIPGL KRTAGLESVQ 

      1090       1100       1110       1120 
VVLVEEGSKT GKDLTNGGAE IEVTAPMAEA ALPGQPSFFF TNV 

« Hide

References

« Hide 'large scale' references
[1]"Regulation of the nuclear genes encoding the cytoplasmic and mitochondrial leucyl-tRNA synthetases of Neurospora crassa."
Chow C.M., RajBhandary U.L.
Mol. Cell. Biol. 9:4645-4652(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[4]"Cytoplasmic leucyl-tRNA synthetase of Neurospora crassa is not specified by the leu-5 locus."
Benarous R., Chow C.M., RajBhandary U.L.
Genetics 119:805-814(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-116.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M30473 Genomic DNA. Translation: AAA33593.1.
BX842596 Genomic DNA. Translation: CAE75711.1.
CM002237 Genomic DNA. Translation: EAA33982.1.
X13021 Genomic DNA. Translation: CAA31439.1.
PIRSYNCLC. A33475.
RefSeqXP_963218.1. XM_958125.2.
UniGeneNcr.15478.

3D structure databases

ProteinModelPortalP10857.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5141.NCU09463.1.

Proteomic databases

PRIDEP10857.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000009314; EFNCRP00000009296; EFNCRG00000009295.
GeneID3879366.
KEGGncr:NCU09463.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216621.
KOK01869.
OMADLTNFGA.
OrthoDBEOG7TTQH2.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYLC_NEUCR
AccessionPrimary (citable) accession number: P10857
Secondary accession number(s): Q7RVI3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: April 1, 1990
Last modified: April 16, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries