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P10857

- SYLC_NEUCR

UniProt

P10857 - SYLC_NEUCR

Protein

Leucine--tRNA ligase, cytoplasmic

Gene

leu-6

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei760 – 7601ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. leucine-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication initiation Source: EnsemblFungi
    2. leucyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligase, cytoplasmic (EC:6.1.1.4)
    Alternative name(s):
    Leucyl-tRNA synthetase
    Short name:
    LeuRS
    Gene namesi
    Name:leu-6
    ORF Names:B10K17.030, NCU09463
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 6, Linkage Group II

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11231123Leucine--tRNA ligase, cytoplasmicPRO_0000152153Add
    BLAST

    Proteomic databases

    PRIDEiP10857.

    Interactioni

    Protein-protein interaction databases

    STRINGi5141.NCU09463.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP10857.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi84 – 9411"HIGH" regionAdd
    BLAST
    Motifi757 – 7615"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000216621.
    KOiK01869.
    OMAiHAPFDYL.
    OrthoDBiEOG7TTQH2.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR004493. Leu-tRNA-synth_Ia_arc/euk.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    [Graphical view]
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P10857-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADTAAVAKG VENLSVSASK TKELKGTEKR DTLIEIEKRY QQKWEQEGVF     50
    EVDAPSTAEF PLDAITPDEL RQKHPKFFGT IAYPYMNGRL HAGHAFSFSK 100
    IEYHTGFARM QGKRALFPQG YHCTGLPIKA SADKLVKEIE MFGQEFERYK 150
    EDEVVEGAAP AAAAAPKTKE DLTKFNAKKG KTVAKTGGAK YQFQILKSLG 200
    IPVSEIHKFA DPQYWLHYFP PECKKDLTNF GARIDWRRQF VTTDANPYYD 250
    AFVRWQMNRL LELNKIKFGK RYTIYSIKDG QPCMDHDRSE GEGVLPQEYT 300
    ALKLKVTEWA PKAAEALKGK LPEGANVYLC PATLRPETMY GQVCCFVGPA 350
    LKYGVFKAAE NEYFVITERA AKNMAYQGIF EKEGVIEKAA DIVGSDLIGT 400
    LVNAPLSVHK EVYVLPMDTV LATKGTGVVT SVPSDSPDDC AMMTELAKKP 450
    EFYGIQKEWA EKEIVSVIKT PTSDLLAPYL VKKLKINSPK DAKQLLEAKE 500
    LAYKEGFYQG IMNYGDFKGE KVETAKPKVR QQLIDAGDAF AYSEPENKVV 550
    SRSGDECSVA LMDQWYIDYG EDSWRTVLYD YVENKDGKGI NTYYADTQHA 600
    FKGVIGWLKQ WACARTYGLG SKLPWDPNFL VESLSDSTVY MAYYTVAHWL 650
    HRDLFGREKG KGNIGADQMI DEVWDYIFCR TELSDHLVTK SGIPKETLDS 700
    MRREFQYFYP LDIRVSGKDL IPNHLTFWLY NHIALFPREY WPKSVRANGH 750
    LQLNGEKMSK STGNFMTLDD VVKKYGADAA RVALADAGDG ISDSNFVEDV 800
    ADNTILRFYT NKEWIEETLK DESLRTGELN SFQDALFDNE MNALVNEARK 850
    HYEETSYKLA LKAAHYDFLN ARDMYREACA AAGIPLHKDL VTKYIRLQAL 900
    VITPIAPHWA DYVWQECLGE PKSIQFARWP EVPAANPALT AARDYVRTTS 950
    SAINSAEAAQ LKKMAKGRQS DFDPKKPKKL TIFATENFPT WQAKYIDLLS 1000
    EVWDAATGTQ KIDDKELNGR IAKMGEMKKA MPFVQALKKR LKDGEPAEQI 1050
    LSRKLSFDEK ATLLAMIPGL KRTAGLESVQ VVLVEEGSKT GKDLTNGGAE 1100
    IEVTAPMAEA ALPGQPSFFF TNV 1123
    Length:1,123
    Mass (Da):126,399
    Last modified:April 1, 1990 - v2
    Checksum:i78945AFC3D06FFBF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M30473 Genomic DNA. Translation: AAA33593.1.
    BX842596 Genomic DNA. Translation: CAE75711.1.
    CM002237 Genomic DNA. Translation: EAA33982.1.
    X13021 Genomic DNA. Translation: CAA31439.1.
    PIRiA33475. SYNCLC.
    RefSeqiXP_963218.1. XM_958125.2.
    UniGeneiNcr.15478.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000009314; EFNCRP00000009296; EFNCRG00000009295.
    GeneIDi3879366.
    KEGGincr:NCU09463.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M30473 Genomic DNA. Translation: AAA33593.1 .
    BX842596 Genomic DNA. Translation: CAE75711.1 .
    CM002237 Genomic DNA. Translation: EAA33982.1 .
    X13021 Genomic DNA. Translation: CAA31439.1 .
    PIRi A33475. SYNCLC.
    RefSeqi XP_963218.1. XM_958125.2.
    UniGenei Ncr.15478.

    3D structure databases

    ProteinModelPortali P10857.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU09463.1.

    Proteomic databases

    PRIDEi P10857.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000009314 ; EFNCRP00000009296 ; EFNCRG00000009295 .
    GeneIDi 3879366.
    KEGGi ncr:NCU09463.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000216621.
    KOi K01869.
    OMAi HAPFDYL.
    OrthoDBi EOG7TTQH2.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR004493. Leu-tRNA-synth_Ia_arc/euk.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsi TIGR00395. leuS_arch. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Regulation of the nuclear genes encoding the cytoplasmic and mitochondrial leucyl-tRNA synthetases of Neurospora crassa."
      Chow C.M., RajBhandary U.L.
      Mol. Cell. Biol. 9:4645-4652(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
      Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
      Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    3. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    4. "Cytoplasmic leucyl-tRNA synthetase of Neurospora crassa is not specified by the leu-5 locus."
      Benarous R., Chow C.M., RajBhandary U.L.
      Genetics 119:805-814(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-116.

    Entry informationi

    Entry nameiSYLC_NEUCR
    AccessioniPrimary (citable) accession number: P10857
    Secondary accession number(s): Q7RVI3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3