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Protein

Leucine--tRNA ligase, cytoplasmic

Gene

leu-6

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei760 – 7601ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. leucine-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication initiation Source: EnsemblFungi
  2. leucyl-tRNA aminoacylation Source: EnsemblFungi
  3. regulation of TOR signaling Source: EnsemblFungi
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase, cytoplasmic (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:leu-6
ORF Names:B10K17.030, NCU09463
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805 Componenti: Chromosome 6, Linkage Group II

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: EnsemblFungi
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11231123Leucine--tRNA ligase, cytoplasmicPRO_0000152153Add
BLAST

Proteomic databases

PRIDEiP10857.

Interactioni

Protein-protein interaction databases

STRINGi5141.NCU09463.1.

Structurei

3D structure databases

ProteinModelPortaliP10857.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi84 – 9411"HIGH" regionAdd
BLAST
Motifi757 – 7615"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216621.
InParanoidiP10857.
KOiK01869.
OMAiSEHFPKT.
OrthoDBiEOG7TTQH2.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10857-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADTAAVAKG VENLSVSASK TKELKGTEKR DTLIEIEKRY QQKWEQEGVF
60 70 80 90 100
EVDAPSTAEF PLDAITPDEL RQKHPKFFGT IAYPYMNGRL HAGHAFSFSK
110 120 130 140 150
IEYHTGFARM QGKRALFPQG YHCTGLPIKA SADKLVKEIE MFGQEFERYK
160 170 180 190 200
EDEVVEGAAP AAAAAPKTKE DLTKFNAKKG KTVAKTGGAK YQFQILKSLG
210 220 230 240 250
IPVSEIHKFA DPQYWLHYFP PECKKDLTNF GARIDWRRQF VTTDANPYYD
260 270 280 290 300
AFVRWQMNRL LELNKIKFGK RYTIYSIKDG QPCMDHDRSE GEGVLPQEYT
310 320 330 340 350
ALKLKVTEWA PKAAEALKGK LPEGANVYLC PATLRPETMY GQVCCFVGPA
360 370 380 390 400
LKYGVFKAAE NEYFVITERA AKNMAYQGIF EKEGVIEKAA DIVGSDLIGT
410 420 430 440 450
LVNAPLSVHK EVYVLPMDTV LATKGTGVVT SVPSDSPDDC AMMTELAKKP
460 470 480 490 500
EFYGIQKEWA EKEIVSVIKT PTSDLLAPYL VKKLKINSPK DAKQLLEAKE
510 520 530 540 550
LAYKEGFYQG IMNYGDFKGE KVETAKPKVR QQLIDAGDAF AYSEPENKVV
560 570 580 590 600
SRSGDECSVA LMDQWYIDYG EDSWRTVLYD YVENKDGKGI NTYYADTQHA
610 620 630 640 650
FKGVIGWLKQ WACARTYGLG SKLPWDPNFL VESLSDSTVY MAYYTVAHWL
660 670 680 690 700
HRDLFGREKG KGNIGADQMI DEVWDYIFCR TELSDHLVTK SGIPKETLDS
710 720 730 740 750
MRREFQYFYP LDIRVSGKDL IPNHLTFWLY NHIALFPREY WPKSVRANGH
760 770 780 790 800
LQLNGEKMSK STGNFMTLDD VVKKYGADAA RVALADAGDG ISDSNFVEDV
810 820 830 840 850
ADNTILRFYT NKEWIEETLK DESLRTGELN SFQDALFDNE MNALVNEARK
860 870 880 890 900
HYEETSYKLA LKAAHYDFLN ARDMYREACA AAGIPLHKDL VTKYIRLQAL
910 920 930 940 950
VITPIAPHWA DYVWQECLGE PKSIQFARWP EVPAANPALT AARDYVRTTS
960 970 980 990 1000
SAINSAEAAQ LKKMAKGRQS DFDPKKPKKL TIFATENFPT WQAKYIDLLS
1010 1020 1030 1040 1050
EVWDAATGTQ KIDDKELNGR IAKMGEMKKA MPFVQALKKR LKDGEPAEQI
1060 1070 1080 1090 1100
LSRKLSFDEK ATLLAMIPGL KRTAGLESVQ VVLVEEGSKT GKDLTNGGAE
1110 1120
IEVTAPMAEA ALPGQPSFFF TNV
Length:1,123
Mass (Da):126,399
Last modified:April 1, 1990 - v2
Checksum:i78945AFC3D06FFBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30473 Genomic DNA. Translation: AAA33593.1.
BX842596 Genomic DNA. Translation: CAE75711.1.
CM002237 Genomic DNA. Translation: EAA33982.1.
X13021 Genomic DNA. Translation: CAA31439.1.
PIRiA33475. SYNCLC.
RefSeqiXP_963218.1. XM_958125.2.
UniGeneiNcr.15478.

Genome annotation databases

EnsemblFungiiEFNCRT00000009314; EFNCRP00000009296; EFNCRG00000009295.
GeneIDi3879366.
KEGGincr:NCU09463.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30473 Genomic DNA. Translation: AAA33593.1.
BX842596 Genomic DNA. Translation: CAE75711.1.
CM002237 Genomic DNA. Translation: EAA33982.1.
X13021 Genomic DNA. Translation: CAA31439.1.
PIRiA33475. SYNCLC.
RefSeqiXP_963218.1. XM_958125.2.
UniGeneiNcr.15478.

3D structure databases

ProteinModelPortaliP10857.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5141.NCU09463.1.

Proteomic databases

PRIDEiP10857.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEFNCRT00000009314; EFNCRP00000009296; EFNCRG00000009295.
GeneIDi3879366.
KEGGincr:NCU09463.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216621.
InParanoidiP10857.
KOiK01869.
OMAiSEHFPKT.
OrthoDBiEOG7TTQH2.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Regulation of the nuclear genes encoding the cytoplasmic and mitochondrial leucyl-tRNA synthetases of Neurospora crassa."
    Chow C.M., RajBhandary U.L.
    Mol. Cell. Biol. 9:4645-4652(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
    Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
    Nucleic Acids Res. 31:1944-1954(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  3. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  4. "Cytoplasmic leucyl-tRNA synthetase of Neurospora crassa is not specified by the leu-5 locus."
    Benarous R., Chow C.M., RajBhandary U.L.
    Genetics 119:805-814(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-116.

Entry informationi

Entry nameiSYLC_NEUCR
AccessioniPrimary (citable) accession number: P10857
Secondary accession number(s): Q7RVI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: April 1, 1990
Last modified: April 1, 2015
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.