ID CCL3_MOUSE Reviewed; 92 AA. AC P10855; P14096; DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot. DT 01-APR-1990, sequence version 2. DT 27-MAR-2024, entry version 192. DE RecName: Full=C-C motif chemokine 3; DE AltName: Full=Heparin-binding chemotaxis protein; DE AltName: Full=L2G25B; DE AltName: Full=Macrophage inflammatory protein 1-alpha; DE Short=MIP-1-alpha; DE AltName: Full=SIS-alpha; DE AltName: Full=Small-inducible cytokine A3; DE AltName: Full=TY-5; DE Flags: Precursor; GN Name=Ccl3; Synonyms=Mip1a, Scya3; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE. RX PubMed=3290382; DOI=10.1084/jem.167.6.1939; RA Davatelis G., Tekamp-Olson P., Wolpe S.D., Hermsen K., Luedke C., RA Gallegos C., Coit D., Merryweather J., Cerami A.; RT "Cloning and characterization of a cDNA for murine macrophage inflammatory RT protein (MIP), a novel monokine with inflammatory and chemokinetic RT properties."; RL J. Exp. Med. 167:1939-1944(1988). RN [2] RP ERRATUM OF PUBMED:3290382, AND SEQUENCE REVISION. RA Davatelis G., Tekamp-Olson P., Wolpe S.D., Hermsen K., Luedke C., RA Gallegos C., Coit D., Merryweather J., Cerami A.; RL J. Exp. Med. 170:2189-2189(1989). RN [3] RP NUCLEOTIDE SEQUENCE [MRNA]. RX PubMed=2521353; RA Brown K.D., Zurawski S.M., Mosmann T.R., Zurawski G.; RT "A family of small inducible proteins secreted by leukocytes are members of RT a new superfamily that includes leukocyte and fibroblast-derived RT inflammatory agents, growth factors, and indicators of various activation RT processes."; RL J. Immunol. 142:679-687(1989). RN [4] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=DBA/2J; RX PubMed=2216738; DOI=10.1093/nar/18.18.5561; RA Grove M., Lowe S., Graham G., Pragnell I., Plumb M.; RT "Sequence of the murine haemopoietic stem cell inhibitor/macrophage RT inflammatory protein 1 alpha gene."; RL Nucleic Acids Res. 18:5561-5561(1990). RN [5] RP NUCLEOTIDE SEQUENCE [MRNA]. RX PubMed=2784565; DOI=10.1073/pnas.86.6.1963; RA Kwon B.S., Weissman S.M.; RT "cDNA sequences of two inducible T-cell genes."; RL Proc. Natl. Acad. Sci. U.S.A. 86:1963-1967(1989). RN [6] RP NUCLEOTIDE SEQUENCE. RX PubMed=2033269; RA Widmer U., Yang Z., van Deventer S., Manogue K.R., Sherry B., Cerami A.; RT "Genomic structure of murine macrophage inflammatory protein-1 alpha and RT conservation of potential regulatory sequences with a human homolog, RT LD78."; RL J. Immunol. 146:4031-4040(1991). RN [7] RP NUCLEOTIDE SEQUENCE. RC STRAIN=B10.S/J, BALB/cJ, DBA/2J, NOD/LtJ, and SJL/J; TISSUE=Spleen; RX PubMed=10438970; RA Teuscher C., Butterfield R.J., Ma R.Z., Zachary J.F., Doerge R.W., RA Blankenhorn E.P.; RT "Sequence polymorphisms in the chemokines Scya1 (TCA-3), Scya2 (monocyte RT chemoattractant protein (MCP)-1), and Scya12 (MCP-5) are candidates for RT eae7, a locus controlling susceptibility to monophasic RT remitting/nonrelapsing experimental allergic encephalomyelitis."; RL J. Immunol. 163:2262-2266(1999). RN [8] RP PROTEIN SEQUENCE OF 24-42. RX PubMed=3279154; DOI=10.1084/jem.167.2.570; RA Wolpe S.D., Davatelis G., Sherry B., Beutler B., Hesse D.G., Nguyen H.T., RA Moldawer L.L., Nathan C.F., Lowry S.F., Cerami A.; RT "Macrophages secrete a novel heparin-binding protein with inflammatory and RT neutrophil chemokinetic properties."; RL J. Exp. Med. 167:570-581(1988). CC -!- FUNCTION: Monokine with inflammatory, pyrogenic and chemokinetic CC properties. Has a potent chemotactic activity for eosinophils. Binding CC to a high-affinity receptor activates calcium release in neutrophils. CC -!- INTERACTION: CC P10855; E9M5R0: RHVP.R17; Xeno; NbExp=4; IntAct=EBI-16188162, EBI-16188152; CC -!- SUBCELLULAR LOCATION: Secreted. CC -!- TISSUE SPECIFICITY: Expressed in lung, spleen, and pancreas. CC -!- SIMILARITY: Belongs to the intercrine beta (chemokine CC) family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M23447; AAA40146.1; -; mRNA. DR EMBL; X12531; CAA31047.1; -; mRNA. DR EMBL; X53372; CAA37452.1; -; Genomic_DNA. DR EMBL; J04491; AAA40304.1; -; mRNA. DR EMBL; M73061; AAA39707.1; -; Genomic_DNA. DR EMBL; AF065939; AAC17506.1; -; mRNA. DR EMBL; AF065940; AAC17507.1; -; mRNA. DR EMBL; AF065941; AAC17508.1; -; mRNA. DR EMBL; AF065942; AAC17509.1; -; mRNA. DR EMBL; AF065943; AAC17510.1; -; mRNA. DR CCDS; CCDS36255.1; -. DR PIR; S11685; A32393. DR RefSeq; NP_035467.1; NM_011337.2. DR PDB; 4ZLT; X-ray; 3.00 A; F/L=24-92. DR PDBsum; 4ZLT; -. DR AlphaFoldDB; P10855; -. DR SMR; P10855; -. DR BioGRID; 203127; 1. DR DIP; DIP-61916N; -. DR IntAct; P10855; 1. DR STRING; 10090.ENSMUSP00000001008; -. DR PaxDb; 10090-ENSMUSP00000001008; -. DR PeptideAtlas; P10855; -. DR DNASU; 20302; -. DR Ensembl; ENSMUST00000001008.6; ENSMUSP00000001008.6; ENSMUSG00000000982.6. DR GeneID; 20302; -. DR KEGG; mmu:20302; -. DR UCSC; uc007kpn.1; mouse. DR AGR; MGI:98260; -. DR CTD; 6348; -. DR MGI; MGI:98260; Ccl3. DR VEuPathDB; HostDB:ENSMUSG00000000982; -. DR eggNOG; ENOG502SAF0; Eukaryota. DR GeneTree; ENSGT01100000263482; -. DR HOGENOM; CLU_141716_4_2_1; -. DR InParanoid; P10855; -. DR OMA; WVQALLE; -. DR OrthoDB; 4265193at2759; -. DR PhylomeDB; P10855; -. DR TreeFam; TF334888; -. DR Reactome; R-MMU-380108; Chemokine receptors bind chemokines. DR BioGRID-ORCS; 20302; 7 hits in 81 CRISPR screens. DR PRO; PR:P10855; -. DR Proteomes; UP000000589; Chromosome 11. DR RNAct; P10855; Protein. DR Bgee; ENSMUSG00000000982; Expressed in granulocyte and 43 other cell types or tissues. DR ExpressionAtlas; P10855; baseline and differential. DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB. DR GO; GO:0005829; C:cytosol; ISS:UniProtKB. DR GO; GO:0005576; C:extracellular region; ISO:MGI. DR GO; GO:0005615; C:extracellular space; IDA:MGI. DR GO; GO:0048020; F:CCR chemokine receptor binding; IBA:GO_Central. DR GO; GO:0031726; F:CCR1 chemokine receptor binding; ISO:MGI. DR GO; GO:0031730; F:CCR5 chemokine receptor binding; ISO:MGI. DR GO; GO:0042056; F:chemoattractant activity; ISS:UniProtKB. DR GO; GO:0008009; F:chemokine activity; IDA:MGI. DR GO; GO:0042802; F:identical protein binding; ISO:MGI. DR GO; GO:0016301; F:kinase activity; ISS:UniProtKB. DR GO; GO:0016004; F:phospholipase activator activity; ISS:UniProtKB. DR GO; GO:0004672; F:protein kinase activity; ISS:UniProtKB. DR GO; GO:0043615; P:astrocyte cell migration; IDA:UniProtKB. DR GO; GO:0006816; P:calcium ion transport; ISS:UniProtKB. DR GO; GO:0019722; P:calcium-mediated signaling; ISS:UniProtKB. DR GO; GO:0001775; P:cell activation; ISS:UniProtKB. DR GO; GO:0007267; P:cell-cell signaling; ISS:UniProtKB. DR GO; GO:0071347; P:cellular response to interleukin-1; IBA:GO_Central. DR GO; GO:0071356; P:cellular response to tumor necrosis factor; IBA:GO_Central. DR GO; GO:0071346; P:cellular response to type II interferon; IBA:GO_Central. DR GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central. DR GO; GO:0006935; P:chemotaxis; ISS:UniProtKB. DR GO; GO:0007010; P:cytoskeleton organization; ISS:UniProtKB. DR GO; GO:0048245; P:eosinophil chemotaxis; ISS:UniProtKB. DR GO; GO:0043308; P:eosinophil degranulation; ISS:UniProtKB. DR GO; GO:0006887; P:exocytosis; ISS:UniProtKB. DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central. DR GO; GO:0071621; P:granulocyte chemotaxis; ISS:UniProtKB. DR GO; GO:0006954; P:inflammatory response; ISS:UniProtKB. DR GO; GO:0006874; P:intracellular calcium ion homeostasis; ISS:UniProtKB. DR GO; GO:0030595; P:leukocyte chemotaxis; ISO:MGI. DR GO; GO:0048247; P:lymphocyte chemotaxis; ISS:UniProtKB. DR GO; GO:0048246; P:macrophage chemotaxis; IGI:UniProtKB. DR GO; GO:0002548; P:monocyte chemotaxis; ISS:UniProtKB. DR GO; GO:0043922; P:negative regulation by host of viral transcription; ISS:UniProtKB. DR GO; GO:0010629; P:negative regulation of gene expression; ISS:UniProtKB. DR GO; GO:0045671; P:negative regulation of osteoclast differentiation; ISS:UniProtKB. DR GO; GO:0030593; P:neutrophil chemotaxis; ISS:UniProtKB. DR GO; GO:0001649; P:osteoblast differentiation; ISS:UniProtKB. DR GO; GO:0051928; P:positive regulation of calcium ion transport; ISS:UniProtKB. DR GO; GO:0050850; P:positive regulation of calcium-mediated signaling; ISS:UniProtKB. DR GO; GO:0030335; P:positive regulation of cell migration; ISS:UniProtKB. DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; ISO:MGI. DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; ISS:UniProtKB. DR GO; GO:0010628; P:positive regulation of gene expression; ISS:UniProtKB. DR GO; GO:0050729; P:positive regulation of inflammatory response; IGI:UniProtKB. DR GO; GO:0032731; P:positive regulation of interleukin-1 beta production; IGI:UniProtKB. DR GO; GO:2000503; P:positive regulation of natural killer cell chemotaxis; ISS:UniProtKB. DR GO; GO:0043525; P:positive regulation of neuron apoptotic process; IGI:UniProtKB. DR GO; GO:0045672; P:positive regulation of osteoclast differentiation; ISO:MGI. DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:UniProtKB. DR GO; GO:0032760; P:positive regulation of tumor necrosis factor production; IGI:UniProtKB. DR GO; GO:0006468; P:protein phosphorylation; ISS:UniProtKB. DR GO; GO:0008360; P:regulation of cell shape; IDA:UniProtKB. DR GO; GO:0043067; P:regulation of programmed cell death; ISO:MGI. DR GO; GO:0051930; P:regulation of sensory perception of pain; ISS:UniProtKB. DR GO; GO:0014808; P:release of sequestered calcium ion into cytosol by sarcoplasmic reticulum; ISS:UniProtKB. DR GO; GO:0070723; P:response to cholesterol; ISS:UniProtKB. DR GO; GO:0009636; P:response to toxic substance; ISS:UniProtKB. DR GO; GO:0009410; P:response to xenobiotic stimulus; IEA:Ensembl. DR GO; GO:0023052; P:signaling; ISS:UniProtKB. DR GO; GO:0010818; P:T cell chemotaxis; ISS:UniProtKB. DR CDD; cd00272; Chemokine_CC; 1. DR Gene3D; 2.40.50.40; -; 1. DR InterPro; IPR039809; Chemokine_b/g/d. DR InterPro; IPR000827; Chemokine_CC_CS. DR InterPro; IPR001811; Chemokine_IL8-like_dom. DR InterPro; IPR036048; Interleukin_8-like_sf. DR PANTHER; PTHR12015:SF183; C-C MOTIF CHEMOKINE 3; 1. DR PANTHER; PTHR12015; SMALL INDUCIBLE CYTOKINE A; 1. DR Pfam; PF00048; IL8; 1. DR SMART; SM00199; SCY; 1. DR SUPFAM; SSF54117; Interleukin 8-like chemokines; 1. DR PROSITE; PS00472; SMALL_CYTOKINES_CC; 1. DR Genevisible; P10855; MM. PE 1: Evidence at protein level; KW 3D-structure; Chemotaxis; Cytokine; Direct protein sequencing; KW Disulfide bond; Inflammatory response; Reference proteome; Secreted; KW Signal. FT SIGNAL 1..23 FT /evidence="ECO:0000269|PubMed:3279154" FT CHAIN 24..92 FT /note="C-C motif chemokine 3" FT /id="PRO_0000005158" FT DISULFID 34..57 FT /evidence="ECO:0000250|UniProtKB:P10147" FT DISULFID 35..73 FT /evidence="ECO:0000250|UniProtKB:P10147" FT CONFLICT 22 FT /note="F -> L (in Ref. 3; AAA40146)" FT /evidence="ECO:0000305" FT CONFLICT 62 FT /note="V -> A (in Ref. 3; AAA40146)" FT /evidence="ECO:0000305" FT STRAND 34..37 FT /evidence="ECO:0007829|PDB:4ZLT" FT HELIX 44..46 FT /evidence="ECO:0007829|PDB:4ZLT" FT STRAND 47..52 FT /evidence="ECO:0007829|PDB:4ZLT" FT STRAND 57..59 FT /evidence="ECO:0007829|PDB:4ZLT" FT STRAND 61..66 FT /evidence="ECO:0007829|PDB:4ZLT" FT STRAND 71..75 FT /evidence="ECO:0007829|PDB:4ZLT" FT HELIX 79..89 FT /evidence="ECO:0007829|PDB:4ZLT" SQ SEQUENCE 92 AA; 10345 MW; 8BFF2DE7C6DEDD38 CRC64; MKVSTTALAV LLCTMTLCNQ VFSAPYGADT PTACCFSYSR KIPRQFIVDY FETSSLCSQP GVIFLTKRNR QICADSKETW VQEYITDLEL NA //