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P10855 (CCL3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-C motif chemokine 3
Alternative name(s):
Heparin-binding chemotaxis protein
L2G25B
Macrophage inflammatory protein 1-alpha
Short name=MIP-1-alpha
SIS-alpha
Small-inducible cytokine A3
TY-5
Gene names
Name:Ccl3
Synonyms:Mip1a, Scya3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length92 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Monokine with inflammatory, pyrogenic and chemokinetic properties. Has a potent chemotactic activity for eosinophils. Binding to a high-affinity receptor activates calcium release in neutrophils.

Subcellular location

Secreted.

Tissue specificity

Expressed in lung, spleen, and pancreas.

Sequence similarities

Belongs to the intercrine beta (chemokine CC) family.

Ontologies

Keywords
   Biological processChemotaxis
Inflammatory response
   Cellular componentSecreted
   DomainSignal
   Molecular functionCytokine
   PTMDisulfide bond
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processMAPK cascade

Inferred from sequence or structural similarity. Source: UniProtKB

T cell chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

astrocyte cell migration

Inferred from direct assay PubMed 15155903. Source: UniProtKB

behavior

Inferred from sequence or structural similarity. Source: UniProtKB

calcium ion transport

Inferred from sequence or structural similarity. Source: UniProtKB

calcium-mediated signaling

Inferred from sequence or structural similarity. Source: UniProtKB

cell activation

Inferred from sequence or structural similarity. Source: UniProtKB

cell-cell signaling

Inferred from sequence or structural similarity. Source: UniProtKB

cellular calcium ion homeostasis

Inferred from sequence or structural similarity. Source: UniProtKB

cellular response to organic cyclic compound

Inferred from sequence or structural similarity. Source: UniProtKB

chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

cytoskeleton organization

Inferred from sequence or structural similarity. Source: UniProtKB

eosinophil chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

eosinophil degranulation

Inferred from sequence or structural similarity. Source: UniProtKB

exocytosis

Inferred from sequence or structural similarity. Source: UniProtKB

granulocyte chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

inflammatory response

Inferred from sequence or structural similarity. Source: UniProtKB

lipopolysaccharide-mediated signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

lymphocyte chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

macrophage chemotaxis

Inferred from genetic interaction PubMed 21784977. Source: UniProtKB

monocyte chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation by host of viral transcription

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of gene expression

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of osteoclast differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

neutrophil chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

osteoblast differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

positive chemotaxis

Inferred from sequence or structural similarity. Source: GOC

positive regulation of ERK1 and ERK2 cascade

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of calcium ion transport

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of calcium-mediated signaling

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of catalytic activity

Inferred from sequence or structural similarity. Source: GOC

positive regulation of cell migration

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of gene expression

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of inflammatory response

Inferred from genetic interaction PubMed 21784977. Source: UniProtKB

positive regulation of interleukin-1 beta secretion

Inferred from genetic interaction PubMed 21784977. Source: UniProtKB

positive regulation of natural killer cell chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of neuron apoptotic process

Inferred from genetic interaction PubMed 15155903. Source: UniProtKB

positive regulation of osteoclast differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 15155903. Source: UniProtKB

positive regulation of tumor necrosis factor production

Inferred from genetic interaction PubMed 21784977. Source: UniProtKB

protein kinase B signaling

Inferred from sequence or structural similarity. Source: UniProtKB

protein phosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of cell shape

Inferred from direct assay PubMed 15155903. Source: UniProtKB

regulation of sensory perception of pain

Inferred from sequence or structural similarity. Source: UniProtKB

release of sequestered calcium ion into cytosol by sarcoplasmic reticulum

Inferred from sequence or structural similarity. Source: UniProtKB

response to cholesterol

Inferred from sequence or structural similarity. Source: UniProtKB

response to drug

Inferred from electronic annotation. Source: Ensembl

response to toxic substance

Inferred from sequence or structural similarity. Source: UniProtKB

signaling

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

cytosol

Inferred from sequence or structural similarity. Source: UniProtKB

extracellular space

Inferred from direct assay PubMed 19139201PubMed 23460747. Source: MGI

intracellular

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functioncalcium-dependent protein kinase C activity

Inferred from sequence or structural similarity. Source: UniProtKB

chemoattractant activity

Inferred from sequence or structural similarity. Source: UniProtKB

chemokine activity

Inferred from direct assay PubMed 7594543. Source: MGI

kinase activity

Inferred from sequence or structural similarity. Source: UniProtKB

phospholipase activator activity

Inferred from sequence or structural similarity. Source: UniProtKB

protein binding

Inferred from physical interaction PubMed 7594543PubMed 9139699. Source: MGI

protein kinase activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Ref.8
Chain24 – 9269C-C motif chemokine 3
PRO_0000005158

Amino acid modifications

Disulfide bond34 ↔ 57 By similarity
Disulfide bond35 ↔ 73 By similarity

Experimental info

Sequence conflict221F → L in AAA40146. Ref.3
Sequence conflict621V → A in AAA40146. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P10855 [UniParc].

Last modified April 1, 1990. Version 2.
Checksum: 8BFF2DE7C6DEDD38

FASTA9210,345
        10         20         30         40         50         60 
MKVSTTALAV LLCTMTLCNQ VFSAPYGADT PTACCFSYSR KIPRQFIVDY FETSSLCSQP 

        70         80         90 
GVIFLTKRNR QICADSKETW VQEYITDLEL NA 

« Hide

References

[1]"Cloning and characterization of a cDNA for murine macrophage inflammatory protein (MIP), a novel monokine with inflammatory and chemokinetic properties."
Davatelis G., Tekamp-Olson P., Wolpe S.D., Hermsen K., Luedke C., Gallegos C., Coit D., Merryweather J., Cerami A.
J. Exp. Med. 167:1939-1944(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]Erratum
Davatelis G., Tekamp-Olson P., Wolpe S.D., Hermsen K., Luedke C., Gallegos C., Coit D., Merryweather J., Cerami A.
J. Exp. Med. 170:2189-2189(1989)
Cited for: SEQUENCE REVISION.
[3]"A family of small inducible proteins secreted by leukocytes are members of a new superfamily that includes leukocyte and fibroblast-derived inflammatory agents, growth factors, and indicators of various activation processes."
Brown K.D., Zurawski S.M., Mosmann T.R., Zurawski G.
J. Immunol. 142:679-687(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Sequence of the murine haemopoietic stem cell inhibitor/macrophage inflammatory protein 1 alpha gene."
Grove M., Lowe S., Graham G., Pragnell I., Plumb M.
Nucleic Acids Res. 18:5561-5561(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DBA/2J.
[5]"cDNA sequences of two inducible T-cell genes."
Kwon B.S., Weissman S.M.
Proc. Natl. Acad. Sci. U.S.A. 86:1963-1967(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]"Genomic structure of murine macrophage inflammatory protein-1 alpha and conservation of potential regulatory sequences with a human homolog, LD78."
Widmer U., Yang Z., van Deventer S., Manogue K.R., Sherry B., Cerami A.
J. Immunol. 146:4031-4040(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[7]"Sequence polymorphisms in the chemokines Scya1 (TCA-3), Scya2 (monocyte chemoattractant protein (MCP)-1), and Scya12 (MCP-5) are candidates for eae7, a locus controlling susceptibility to monophasic remitting/nonrelapsing experimental allergic encephalomyelitis."
Teuscher C., Butterfield R.J., Ma R.Z., Zachary J.F., Doerge R.W., Blankenhorn E.P.
J. Immunol. 163:2262-2266(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: B10.S/J, BALB/cJ, DBA/2J, NOD/LtJ and SJL/J.
Tissue: Spleen.
[8]"Macrophages secrete a novel heparin-binding protein with inflammatory and neutrophil chemokinetic properties."
Wolpe S.D., Davatelis G., Sherry B., Beutler B., Hesse D.G., Nguyen H.T., Moldawer L.L., Nathan C.F., Lowry S.F., Cerami A.
J. Exp. Med. 167:570-581(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 24-42.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M23447 mRNA. Translation: AAA40146.1.
X12531 mRNA. Translation: CAA31047.1.
X53372 Genomic DNA. Translation: CAA37452.1.
J04491 mRNA. Translation: AAA40304.1.
M73061 Genomic DNA. Translation: AAA39707.1.
AF065939 mRNA. Translation: AAC17506.1.
AF065940 mRNA. Translation: AAC17507.1.
AF065941 mRNA. Translation: AAC17508.1.
AF065942 mRNA. Translation: AAC17509.1.
AF065943 mRNA. Translation: AAC17510.1.
CCDSCCDS36255.1.
PIRA32393. S11685.
RefSeqNP_035467.1. NM_011337.2.
UniGeneMm.1282.

3D structure databases

ProteinModelPortalP10855.
SMRP10855. Positions 28-92.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203127. 1 interaction.

Proteomic databases

PRIDEP10855.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000001008; ENSMUSP00000001008; ENSMUSG00000000982.
GeneID20302.
KEGGmmu:20302.
UCSCuc007kpn.1. mouse.

Organism-specific databases

CTD6348.
MGIMGI:98260. Ccl3.

Phylogenomic databases

eggNOGNOG42742.
HOGENOMHOG000036685.
HOVERGENHBG017871.
InParanoidP10855.
KOK05408.
OMASKETWVQ.
OrthoDBEOG7CVQ1F.
PhylomeDBP10855.
TreeFamTF334888.

Gene expression databases

ArrayExpressP10855.
BgeeP10855.
CleanExMM_CCL3.
GenevestigatorP10855.

Family and domain databases

InterProIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamPF00048. IL8. 1 hit.
[Graphical view]
SMARTSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMSSF54117. SSF54117. 1 hit.
PROSITEPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio298047.
PROP10855.
SOURCESearch...

Entry information

Entry nameCCL3_MOUSE
AccessionPrimary (citable) accession number: P10855
Secondary accession number(s): P14096
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: April 1, 1990
Last modified: July 9, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot