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Protein

C-C motif chemokine 3

Gene

Ccl3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Monokine with inflammatory, pyrogenic and chemokinetic properties. Has a potent chemotactic activity for eosinophils. Binding to a high-affinity receptor activates calcium release in neutrophils.

GO - Molecular functioni

  1. calcium-dependent protein kinase C activity Source: UniProtKB
  2. chemoattractant activity Source: UniProtKB
  3. chemokine activity Source: MGI
  4. kinase activity Source: UniProtKB
  5. phospholipase activator activity Source: UniProtKB
  6. protein kinase activity Source: UniProtKB

GO - Biological processi

  1. astrocyte cell migration Source: UniProtKB
  2. calcium ion transport Source: UniProtKB
  3. calcium-mediated signaling Source: UniProtKB
  4. cell activation Source: UniProtKB
  5. cell-cell signaling Source: UniProtKB
  6. cellular calcium ion homeostasis Source: UniProtKB
  7. cellular response to organic cyclic compound Source: UniProtKB
  8. chemotaxis Source: UniProtKB
  9. cytoskeleton organization Source: UniProtKB
  10. eosinophil chemotaxis Source: UniProtKB
  11. eosinophil degranulation Source: UniProtKB
  12. exocytosis Source: UniProtKB
  13. granulocyte chemotaxis Source: UniProtKB
  14. inflammatory response Source: UniProtKB
  15. lipopolysaccharide-mediated signaling pathway Source: UniProtKB
  16. lymphocyte chemotaxis Source: UniProtKB
  17. macrophage chemotaxis Source: UniProtKB
  18. MAPK cascade Source: UniProtKB
  19. monocyte chemotaxis Source: UniProtKB
  20. negative regulation by host of viral transcription Source: UniProtKB
  21. negative regulation of gene expression Source: UniProtKB
  22. negative regulation of osteoclast differentiation Source: UniProtKB
  23. neutrophil chemotaxis Source: UniProtKB
  24. osteoblast differentiation Source: UniProtKB
  25. positive chemotaxis Source: GOC
  26. positive regulation of calcium ion transport Source: UniProtKB
  27. positive regulation of calcium-mediated signaling Source: UniProtKB
  28. positive regulation of catalytic activity Source: GOC
  29. positive regulation of cell migration Source: UniProtKB
  30. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  31. positive regulation of gene expression Source: UniProtKB
  32. positive regulation of inflammatory response Source: UniProtKB
  33. positive regulation of interleukin-1 beta secretion Source: UniProtKB
  34. positive regulation of natural killer cell chemotaxis Source: UniProtKB
  35. positive regulation of neuron apoptotic process Source: UniProtKB
  36. positive regulation of osteoclast differentiation Source: Ensembl
  37. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  38. positive regulation of tumor necrosis factor production Source: UniProtKB
  39. protein kinase B signaling Source: UniProtKB
  40. protein phosphorylation Source: UniProtKB
  41. regulation of cell shape Source: UniProtKB
  42. regulation of sensory perception of pain Source: UniProtKB
  43. release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: UniProtKB
  44. response to cholesterol Source: UniProtKB
  45. response to drug Source: Ensembl
  46. response to toxic substance Source: UniProtKB
  47. signaling Source: UniProtKB
  48. T cell chemotaxis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Chemotaxis, Inflammatory response

Enzyme and pathway databases

ReactomeiREACT_230248. Chemokine receptors bind chemokines.

Names & Taxonomyi

Protein namesi
Recommended name:
C-C motif chemokine 3
Alternative name(s):
Heparin-binding chemotaxis protein
L2G25B
Macrophage inflammatory protein 1-alpha
Short name:
MIP-1-alpha
SIS-alpha
Small-inducible cytokine A3
TY-5
Gene namesi
Name:Ccl3
Synonyms:Mip1a, Scya3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:98260. Ccl3.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytosol Source: UniProtKB
  3. extracellular space Source: MGI
  4. intracellular Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 9269C-C motif chemokine 3PRO_0000005158Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi34 ↔ 57By similarity
Disulfide bondi35 ↔ 73By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP10855.

Expressioni

Tissue specificityi

Expressed in lung, spleen, and pancreas.

Gene expression databases

BgeeiP10855.
CleanExiMM_CCL3.
ExpressionAtlasiP10855. baseline and differential.
GenevestigatoriP10855.

Interactioni

Protein-protein interaction databases

BioGridi203127. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP10855.
SMRiP10855. Positions 28-92.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG42742.
HOGENOMiHOG000036685.
HOVERGENiHBG017871.
InParanoidiP10855.
KOiK05408.
OMAiSAPFGAD.
OrthoDBiEOG7CVQ1F.
PhylomeDBiP10855.
TreeFamiTF334888.

Family and domain databases

InterProiIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10855-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVSTTALAV LLCTMTLCNQ VFSAPYGADT PTACCFSYSR KIPRQFIVDY
60 70 80 90
FETSSLCSQP GVIFLTKRNR QICADSKETW VQEYITDLEL NA
Length:92
Mass (Da):10,345
Last modified:April 1, 1990 - v2
Checksum:i8BFF2DE7C6DEDD38
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221F → L in AAA40146. (PubMed:2521353)Curated
Sequence conflicti62 – 621V → A in AAA40146. (PubMed:2521353)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23447 mRNA. Translation: AAA40146.1.
X12531 mRNA. Translation: CAA31047.1.
X53372 Genomic DNA. Translation: CAA37452.1.
J04491 mRNA. Translation: AAA40304.1.
M73061 Genomic DNA. Translation: AAA39707.1.
AF065939 mRNA. Translation: AAC17506.1.
AF065940 mRNA. Translation: AAC17507.1.
AF065941 mRNA. Translation: AAC17508.1.
AF065942 mRNA. Translation: AAC17509.1.
AF065943 mRNA. Translation: AAC17510.1.
CCDSiCCDS36255.1.
PIRiS11685. A32393.
RefSeqiNP_035467.1. NM_011337.2.
UniGeneiMm.1282.

Genome annotation databases

EnsembliENSMUST00000001008; ENSMUSP00000001008; ENSMUSG00000000982.
GeneIDi20302.
KEGGimmu:20302.
UCSCiuc007kpn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23447 mRNA. Translation: AAA40146.1.
X12531 mRNA. Translation: CAA31047.1.
X53372 Genomic DNA. Translation: CAA37452.1.
J04491 mRNA. Translation: AAA40304.1.
M73061 Genomic DNA. Translation: AAA39707.1.
AF065939 mRNA. Translation: AAC17506.1.
AF065940 mRNA. Translation: AAC17507.1.
AF065941 mRNA. Translation: AAC17508.1.
AF065942 mRNA. Translation: AAC17509.1.
AF065943 mRNA. Translation: AAC17510.1.
CCDSiCCDS36255.1.
PIRiS11685. A32393.
RefSeqiNP_035467.1. NM_011337.2.
UniGeneiMm.1282.

3D structure databases

ProteinModelPortaliP10855.
SMRiP10855. Positions 28-92.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203127. 1 interaction.

Proteomic databases

PRIDEiP10855.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001008; ENSMUSP00000001008; ENSMUSG00000000982.
GeneIDi20302.
KEGGimmu:20302.
UCSCiuc007kpn.1. mouse.

Organism-specific databases

CTDi6348.
MGIiMGI:98260. Ccl3.

Phylogenomic databases

eggNOGiNOG42742.
HOGENOMiHOG000036685.
HOVERGENiHBG017871.
InParanoidiP10855.
KOiK05408.
OMAiSAPFGAD.
OrthoDBiEOG7CVQ1F.
PhylomeDBiP10855.
TreeFamiTF334888.

Enzyme and pathway databases

ReactomeiREACT_230248. Chemokine receptors bind chemokines.

Miscellaneous databases

NextBioi298047.
PROiP10855.
SOURCEiSearch...

Gene expression databases

BgeeiP10855.
CleanExiMM_CCL3.
ExpressionAtlasiP10855. baseline and differential.
GenevestigatoriP10855.

Family and domain databases

InterProiIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of a cDNA for murine macrophage inflammatory protein (MIP), a novel monokine with inflammatory and chemokinetic properties."
    Davatelis G., Tekamp-Olson P., Wolpe S.D., Hermsen K., Luedke C., Gallegos C., Coit D., Merryweather J., Cerami A.
    J. Exp. Med. 167:1939-1944(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. Cited for: SEQUENCE REVISION.
  3. "A family of small inducible proteins secreted by leukocytes are members of a new superfamily that includes leukocyte and fibroblast-derived inflammatory agents, growth factors, and indicators of various activation processes."
    Brown K.D., Zurawski S.M., Mosmann T.R., Zurawski G.
    J. Immunol. 142:679-687(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Sequence of the murine haemopoietic stem cell inhibitor/macrophage inflammatory protein 1 alpha gene."
    Grove M., Lowe S., Graham G., Pragnell I., Plumb M.
    Nucleic Acids Res. 18:5561-5561(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DBA/2J.
  5. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  6. "Genomic structure of murine macrophage inflammatory protein-1 alpha and conservation of potential regulatory sequences with a human homolog, LD78."
    Widmer U., Yang Z., van Deventer S., Manogue K.R., Sherry B., Cerami A.
    J. Immunol. 146:4031-4040(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  7. "Sequence polymorphisms in the chemokines Scya1 (TCA-3), Scya2 (monocyte chemoattractant protein (MCP)-1), and Scya12 (MCP-5) are candidates for eae7, a locus controlling susceptibility to monophasic remitting/nonrelapsing experimental allergic encephalomyelitis."
    Teuscher C., Butterfield R.J., Ma R.Z., Zachary J.F., Doerge R.W., Blankenhorn E.P.
    J. Immunol. 163:2262-2266(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: B10.S/J, BALB/cJ, DBA/2J, NOD/LtJ and SJL/J.
    Tissue: Spleen.
  8. "Macrophages secrete a novel heparin-binding protein with inflammatory and neutrophil chemokinetic properties."
    Wolpe S.D., Davatelis G., Sherry B., Beutler B., Hesse D.G., Nguyen H.T., Moldawer L.L., Nathan C.F., Lowry S.F., Cerami A.
    J. Exp. Med. 167:570-581(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-42.

Entry informationi

Entry nameiCCL3_MOUSE
AccessioniPrimary (citable) accession number: P10855
Secondary accession number(s): P14096
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: April 1, 1990
Last modified: January 7, 2015
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.