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Protein

Histone H2B type 1-F/J/L

Gene

Hist1h2bf

more
Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-1221633. Meiotic Synapsis.
R-MMU-573389. NoRC negatively regulates rRNA expression.
R-MMU-912497. Meiotic Recombination.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H2B type 1-F/J/L
Alternative name(s):
H2B 291A
Gene namesi
Name:Hist1h2bf
Synonyms:H2b-f
AND
Name:Hist1h2bj
Synonyms:H2b-j
AND
Name:Hist1h2bl
Synonyms:H2b-l
AND
Name:Hist1h2bn
Synonyms:H2b-n
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2448383. Hist1h2bf.
MGI:2448388. Hist1h2bj.
MGI:2448403. Hist1h2bl.
MGI:2448407. Hist1h2bn.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000718392 – 126Histone H2B type 1-F/J/LAdd BLAST125

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylprolineBy similarity1
Modified residuei6N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei6N6-(beta-hydroxybutyryl)lysine; alternate1 Publication1
Modified residuei6N6-acetyllysine; alternateBy similarity1
Modified residuei6N6-butyryllysine; alternateBy similarity1
Modified residuei6N6-crotonyllysine; alternate1 Publication1
Cross-linki6Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei12N6-(beta-hydroxybutyryl)lysine; alternate1 Publication1
Modified residuei12N6-acetyllysine; alternateBy similarity1
Modified residuei12N6-crotonyllysine; alternate1 Publication1
Modified residuei13N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei13N6-acetyllysine; alternateBy similarity1
Modified residuei13N6-crotonyllysine; alternate1 Publication1
Modified residuei15Phosphoserine; by STK4/MST12 Publications1
Modified residuei16N6-acetyllysine; alternateBy similarity1
Modified residuei16N6-crotonyllysine; alternate1 Publication1
Modified residuei17N6-acetyllysine; alternateBy similarity1
Modified residuei17N6-crotonyllysine; alternate1 Publication1
Modified residuei21N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei21N6-(beta-hydroxybutyryl)lysine; alternate1 Publication1
Modified residuei21N6-acetyllysine; alternateBy similarity1
Modified residuei21N6-butyryllysine; alternateBy similarity1
Modified residuei21N6-crotonyllysine; alternate1 Publication1
Cross-linki21Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei24N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei24N6-acetyllysine; alternateBy similarity1
Modified residuei24N6-crotonyllysine; alternate1 Publication1
Modified residuei25N6-(2-hydroxyisobutyryl)lysine1 Publication1
Modified residuei35N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei35N6-(beta-hydroxybutyryl)lysine; alternate1 Publication1
Modified residuei35N6-crotonyllysine; alternate1 Publication1
Cross-linki35Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei37Phosphoserine; by AMPK1 Publication1
Modified residuei44N6-(2-hydroxyisobutyryl)lysine1 Publication1
Modified residuei47N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei47N6-methyllysine; alternateBy similarity1
Modified residuei58N6,N6-dimethyllysine; alternateBy similarity1
Modified residuei58N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei80Dimethylated arginineBy similarity1
Modified residuei86N6,N6,N6-trimethyllysine; alternateBy similarity1
Modified residuei86N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei86N6-acetyllysine; alternateBy similarity1
Modified residuei87Omega-N-methylarginineBy similarity1
Modified residuei93Omega-N-methylarginineBy similarity1
Modified residuei109N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei109N6-(beta-hydroxybutyryl)lysine; alternate1 Publication1
Modified residuei109N6-methyllysine; alternateBy similarity1
Glycosylationi113O-linked (GlcNAc) serineBy similarity1
Modified residuei116PhosphothreonineBy similarity1
Modified residuei117N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei117N6-(beta-hydroxybutyryl)lysine; alternate1 Publication1
Modified residuei117N6-methylated lysineBy similarity1
Modified residuei121N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei121N6-succinyllysine; alternate1 Publication1
Cross-linki121Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons (By similarity).By similarity
Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription.3 Publications
GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes (By similarity).By similarity
Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.1 Publication
Hydroxybutyrylation of histones is induced by starvation.1 Publication

Keywords - PTMi

Acetylation, Glycoprotein, Hydroxylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP10853.
PaxDbiP10853.
PeptideAtlasiP10853.
PRIDEiP10853.
TopDownProteomicsiP10853.

PTM databases

iPTMnetiP10853.
PhosphoSitePlusiP10853.

Expressioni

Gene expression databases

BgeeiENSMUSG00000069268.
CleanExiMM_HIST1H2BF.
MM_HIST1H2BJ.
MM_HIST1H2BL.
MM_HIST1H2BN.
ExpressionAtlasiP10853. baseline and differential.
GenevisibleiP10853. MM.

Interactioni

Subunit structurei

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi235104. 1 interactor.
IntActiP10853. 2 interactors.
MINTiMINT-1172975.
STRINGi10090.ENSMUSP00000106082.

Structurei

3D structure databases

ProteinModelPortaliP10853.
SMRiP10853.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histone H2B family.Curated

Phylogenomic databases

eggNOGiKOG1744. Eukaryota.
ENOG4111NV5. LUCA.
GeneTreeiENSGT00760000118976.
HOGENOMiHOG000231213.
HOVERGENiHBG007774.
InParanoidiP10853.
KOiK11252.
OMAiSRCQFES.
OrthoDBiEOG091G0XGD.
PhylomeDBiP10853.
TreeFamiTF300212.

Family and domain databases

InterProiView protein in InterPro
IPR009072. Histone-fold.
IPR007125. Histone_H2A/H2B/H3.
IPR000558. Histone_H2B.
PANTHERiPTHR23428. PTHR23428. 1 hit.
PfamiView protein in Pfam
PF00125. Histone. 1 hit.
PRINTSiPR00621. HISTONEH2B.
SMARTiView protein in SMART
SM00427. H2B. 1 hit.
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiView protein in PROSITE
PS00357. HISTONE_H2B. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10853-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEPAKSAPA PKKGSKKAVT KAQKKDGKKR KRSRKESYSV YVYKVLKQVH
60 70 80 90 100
PDTGISSKAM GIMNSFVNDI FERIASEASR LAHYNKRSTI TSREIQTAVR
110 120
LLLPGELAKH AVSEGTKAVT KYTSSK
Length:126
Mass (Da):13,936
Last modified:January 23, 2007 - v2
Checksum:iFAE15CE99FC8B03D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05862 Genomic DNA. Translation: CAA29290.1.
U62669 Genomic DNA. Translation: AAB04762.1.
AY158927 Genomic DNA. Translation: AAO06237.1.
AY158929 Genomic DNA. Translation: AAO06239.1.
AY158932 Genomic DNA. Translation: AAO06242.1.
AY158935 Genomic DNA. Translation: AAO06245.1.
AL589651 Genomic DNA. Translation: CAI24103.1.
AL589651 Genomic DNA. Translation: CAI24111.1.
AL589879 Genomic DNA. Translation: CAI25462.1.
AL589879 Genomic DNA. Translation: CAI25467.1.
AL590614 Genomic DNA. Translation: CAI26130.1.
CCDSiCCDS26287.1.
CCDS26294.1.
CCDS26308.1.
CCDS26350.1.
PIRiS04151.
RefSeqiNP_001300807.1. NM_001313878.1.
NP_001300809.1. NM_001313880.1.
NP_835502.1. NM_178195.2.
NP_835505.1. NM_178198.2.
NP_835506.1. NM_178199.2.
NP_835508.1. NM_178201.2.
UniGeneiMm.371766.
Mm.471247.

Genome annotation databases

EnsembliENSMUST00000078156; ENSMUSP00000077290; ENSMUSG00000069307.
ENSMUST00000091709; ENSMUSP00000089301; ENSMUSG00000095217.
ENSMUST00000091756; ENSMUSP00000089350; ENSMUSG00000094338.
ENSMUST00000105106; ENSMUSP00000100738; ENSMUSG00000069268.
ENSMUST00000110452; ENSMUSP00000106082; ENSMUSG00000069300.
ENSMUST00000110467; ENSMUSP00000106093; ENSMUSG00000069303.
ENSMUST00000110469; ENSMUSP00000106095; ENSMUSG00000069307.
ENSMUST00000110476; ENSMUSP00000106102; ENSMUSG00000069303.
GeneIDi319180.
319183.
319185.
319187.
665596.
665622.
KEGGimmu:319180.
mmu:319183.
mmu:319185.
mmu:319187.
mmu:665596.
mmu:665622.
UCSCiuc007pra.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiH2B1F_MOUSE
AccessioniPrimary (citable) accession number: P10853
Secondary accession number(s): Q5SZZ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families