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Protein

Histone H2B type 1-F/J/L

Gene

Hist1h2bf

more
Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-1221632. Meiotic synapsis.
R-MMU-1221633. Meiotic Synapsis.
R-MMU-3214847. HATs acetylate histones.
R-MMU-427389. ERCC6 (CSB) and EHMT2 (G9a) positively regulate rRNA expression.
R-MMU-427413. NoRC negatively regulates rRNA expression.
R-MMU-5334118. DNA methylation.
R-MMU-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
R-MMU-573389. NoRC negatively regulates rRNA expression.
R-MMU-912497. Meiotic Recombination.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H2B type 1-F/J/L
Alternative name(s):
H2B 291A
Gene namesi
Name:Hist1h2bf
Synonyms:H2b-f
AND
Name:Hist1h2bj
Synonyms:H2b-j
AND
Name:Hist1h2bl
Synonyms:H2b-l
AND
Name:Hist1h2bn
Synonyms:H2b-n
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2448383. Hist1h2bf.
MGI:2448388. Hist1h2bj.
MGI:2448403. Hist1h2bl.
MGI:2448407. Hist1h2bn.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000718392 – 126Histone H2B type 1-F/J/LAdd BLAST125

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylprolineBy similarity1
Modified residuei6N6-acetyllysine; alternateBy similarity1
Modified residuei6N6-crotonyllysine; alternate1 Publication1
Cross-linki6Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei12N6-acetyllysine; alternateBy similarity1
Modified residuei12N6-crotonyllysine; alternate1 Publication1
Modified residuei13N6-acetyllysine; alternateBy similarity1
Modified residuei13N6-crotonyllysine; alternate1 Publication1
Modified residuei15Phosphoserine; by STK4/MST12 Publications1
Modified residuei16N6-acetyllysine; alternateBy similarity1
Modified residuei16N6-crotonyllysine; alternate1 Publication1
Modified residuei17N6-acetyllysine; alternateBy similarity1
Modified residuei17N6-crotonyllysine; alternate1 Publication1
Modified residuei21N6-acetyllysine; alternateBy similarity1
Modified residuei21N6-crotonyllysine; alternate1 Publication1
Modified residuei24N6-acetyllysine; alternateBy similarity1
Modified residuei24N6-crotonyllysine; alternate1 Publication1
Modified residuei35N6-crotonyllysine; alternate1 Publication1
Cross-linki35Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei37Phosphoserine; by AMPK1 Publication1
Modified residuei47N6-methyllysineBy similarity1
Modified residuei58N6,N6-dimethyllysineBy similarity1
Modified residuei80Dimethylated arginineBy similarity1
Modified residuei86N6,N6,N6-trimethyllysine; alternateBy similarity1
Modified residuei86N6-acetyllysine; alternateBy similarity1
Modified residuei87Omega-N-methylarginineBy similarity1
Modified residuei93Omega-N-methylarginineBy similarity1
Modified residuei109N6-methyllysineBy similarity1
Glycosylationi113O-linked (GlcNAc)By similarity1
Modified residuei116PhosphothreonineBy similarity1
Modified residuei117N6-methylated lysineBy similarity1
Cross-linki121Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons (By similarity).By similarity
Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription.3 Publications
GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes (By similarity).By similarity
Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.1 Publication

Keywords - PTMi

Acetylation, Glycoprotein, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP10853.
PaxDbiP10853.
PeptideAtlasiP10853.
PRIDEiP10853.
TopDownProteomicsiP10853.

PTM databases

iPTMnetiP10853.
PhosphoSitePlusiP10853.

Expressioni

Gene expression databases

BgeeiENSMUSG00000069268.
CleanExiMM_HIST1H2BF.
MM_HIST1H2BJ.
MM_HIST1H2BL.
MM_HIST1H2BN.
ExpressionAtlasiP10853. baseline and differential.
GenevisibleiP10853. MM.

Interactioni

Subunit structurei

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Protein-protein interaction databases

BioGridi235104. 1 interactor.
IntActiP10853. 2 interactors.
MINTiMINT-1172975.
STRINGi10090.ENSMUSP00000106082.

Structurei

3D structure databases

ProteinModelPortaliP10853.
SMRiP10853.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histone H2B family.Curated

Phylogenomic databases

eggNOGiKOG1744. Eukaryota.
ENOG4111NV5. LUCA.
GeneTreeiENSGT00760000118976.
HOGENOMiHOG000231213.
HOVERGENiHBG007774.
InParanoidiP10853.
KOiK11252.
OMAiNESENHF.
OrthoDBiEOG091G0XGD.
PhylomeDBiP10853.
TreeFamiTF300212.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR007125. Histone_H2A/H2B/H3.
IPR000558. Histone_H2B.
[Graphical view]
PANTHERiPTHR23428. PTHR23428. 1 hit.
PfamiPF00125. Histone. 1 hit.
[Graphical view]
PRINTSiPR00621. HISTONEH2B.
SMARTiSM00427. H2B. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS00357. HISTONE_H2B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10853-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEPAKSAPA PKKGSKKAVT KAQKKDGKKR KRSRKESYSV YVYKVLKQVH
60 70 80 90 100
PDTGISSKAM GIMNSFVNDI FERIASEASR LAHYNKRSTI TSREIQTAVR
110 120
LLLPGELAKH AVSEGTKAVT KYTSSK
Length:126
Mass (Da):13,936
Last modified:January 23, 2007 - v2
Checksum:iFAE15CE99FC8B03D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05862 Genomic DNA. Translation: CAA29290.1.
U62669 Genomic DNA. Translation: AAB04762.1.
AY158927 Genomic DNA. Translation: AAO06237.1.
AY158929 Genomic DNA. Translation: AAO06239.1.
AY158932 Genomic DNA. Translation: AAO06242.1.
AY158935 Genomic DNA. Translation: AAO06245.1.
AL589651 Genomic DNA. Translation: CAI24103.1.
AL589651 Genomic DNA. Translation: CAI24111.1.
AL589879 Genomic DNA. Translation: CAI25462.1.
AL589879 Genomic DNA. Translation: CAI25467.1.
AL590614 Genomic DNA. Translation: CAI26130.1.
CCDSiCCDS26287.1.
CCDS26294.1.
CCDS26308.1.
CCDS26350.1.
PIRiS04151.
RefSeqiNP_001300807.1. NM_001313878.1.
NP_001300809.1. NM_001313880.1.
NP_835502.1. NM_178195.2.
NP_835505.1. NM_178198.2.
NP_835506.1. NM_178199.2.
NP_835508.1. NM_178201.2.
UniGeneiMm.371766.
Mm.471247.

Genome annotation databases

EnsembliENSMUST00000091709; ENSMUSP00000089301; ENSMUSG00000095217.
ENSMUST00000091756; ENSMUSP00000089350; ENSMUSG00000094338.
ENSMUST00000105106; ENSMUSP00000100738; ENSMUSG00000069268.
ENSMUST00000110452; ENSMUSP00000106082; ENSMUSG00000069300.
ENSMUST00000110467; ENSMUSP00000106093; ENSMUSG00000069303.
ENSMUST00000110469; ENSMUSP00000106095; ENSMUSG00000069307.
GeneIDi319180.
319183.
319185.
319187.
665596.
665622.
KEGGimmu:319180.
mmu:319183.
mmu:319185.
mmu:319187.
mmu:665596.
mmu:665622.
UCSCiuc007pra.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05862 Genomic DNA. Translation: CAA29290.1.
U62669 Genomic DNA. Translation: AAB04762.1.
AY158927 Genomic DNA. Translation: AAO06237.1.
AY158929 Genomic DNA. Translation: AAO06239.1.
AY158932 Genomic DNA. Translation: AAO06242.1.
AY158935 Genomic DNA. Translation: AAO06245.1.
AL589651 Genomic DNA. Translation: CAI24103.1.
AL589651 Genomic DNA. Translation: CAI24111.1.
AL589879 Genomic DNA. Translation: CAI25462.1.
AL589879 Genomic DNA. Translation: CAI25467.1.
AL590614 Genomic DNA. Translation: CAI26130.1.
CCDSiCCDS26287.1.
CCDS26294.1.
CCDS26308.1.
CCDS26350.1.
PIRiS04151.
RefSeqiNP_001300807.1. NM_001313878.1.
NP_001300809.1. NM_001313880.1.
NP_835502.1. NM_178195.2.
NP_835505.1. NM_178198.2.
NP_835506.1. NM_178199.2.
NP_835508.1. NM_178201.2.
UniGeneiMm.371766.
Mm.471247.

3D structure databases

ProteinModelPortaliP10853.
SMRiP10853.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi235104. 1 interactor.
IntActiP10853. 2 interactors.
MINTiMINT-1172975.
STRINGi10090.ENSMUSP00000106082.

PTM databases

iPTMnetiP10853.
PhosphoSitePlusiP10853.

Proteomic databases

MaxQBiP10853.
PaxDbiP10853.
PeptideAtlasiP10853.
PRIDEiP10853.
TopDownProteomicsiP10853.

Protocols and materials databases

DNASUi319180.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000091709; ENSMUSP00000089301; ENSMUSG00000095217.
ENSMUST00000091756; ENSMUSP00000089350; ENSMUSG00000094338.
ENSMUST00000105106; ENSMUSP00000100738; ENSMUSG00000069268.
ENSMUST00000110452; ENSMUSP00000106082; ENSMUSG00000069300.
ENSMUST00000110467; ENSMUSP00000106093; ENSMUSG00000069303.
ENSMUST00000110469; ENSMUSP00000106095; ENSMUSG00000069307.
GeneIDi319180.
319183.
319185.
319187.
665596.
665622.
KEGGimmu:319180.
mmu:319183.
mmu:319185.
mmu:319187.
mmu:665596.
mmu:665622.
UCSCiuc007pra.2. mouse.

Organism-specific databases

CTDi665596.
665622.
8340.
8341.
8343.
8970.
MGIiMGI:2448383. Hist1h2bf.
MGI:2448388. Hist1h2bj.
MGI:2448403. Hist1h2bl.
MGI:2448407. Hist1h2bn.

Phylogenomic databases

eggNOGiKOG1744. Eukaryota.
ENOG4111NV5. LUCA.
GeneTreeiENSGT00760000118976.
HOGENOMiHOG000231213.
HOVERGENiHBG007774.
InParanoidiP10853.
KOiK11252.
OMAiNESENHF.
OrthoDBiEOG091G0XGD.
PhylomeDBiP10853.
TreeFamiTF300212.

Enzyme and pathway databases

ReactomeiR-MMU-1221632. Meiotic synapsis.
R-MMU-1221633. Meiotic Synapsis.
R-MMU-3214847. HATs acetylate histones.
R-MMU-427389. ERCC6 (CSB) and EHMT2 (G9a) positively regulate rRNA expression.
R-MMU-427413. NoRC negatively regulates rRNA expression.
R-MMU-5334118. DNA methylation.
R-MMU-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
R-MMU-573389. NoRC negatively regulates rRNA expression.
R-MMU-912497. Meiotic Recombination.

Miscellaneous databases

PROiP10853.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000069268.
CleanExiMM_HIST1H2BF.
MM_HIST1H2BJ.
MM_HIST1H2BL.
MM_HIST1H2BN.
ExpressionAtlasiP10853. baseline and differential.
GenevisibleiP10853. MM.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR007125. Histone_H2A/H2B/H3.
IPR000558. Histone_H2B.
[Graphical view]
PANTHERiPTHR23428. PTHR23428. 1 hit.
PfamiPF00125. Histone. 1 hit.
[Graphical view]
PRINTSiPR00621. HISTONEH2B.
SMARTiSM00427. H2B. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS00357. HISTONE_H2B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiH2B1F_MOUSE
AccessioniPrimary (citable) accession number: P10853
Secondary accession number(s): Q5SZZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 159 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.