P10846 (IAA3_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-amylase inhibitor WDAI-3

Gene names

Name:

IHA-B1-2

Organism

Triticum aestivum (Wheat)

Taxonomic identifier

4565 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	44 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Alpha-amylase inhibitor.
Subunit structure	Homodimer.
Subcellular location	Secreted.
Tissue specificity	Endosperm.
Post-translational modification	The disulfide bonds are essential for the inhibitor activity.
Sequence similarities	Belongs to the protease inhibitor I6 (cereal trypsin/alpha-amylase inhibitor) family. [View classification]

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Alpha-amylase inhibitor
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-amylase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›44	›44	Alpha-amylase inhibitor WDAI-3		PRO_0000070487
Amino acid modifications
	Disulfide bond	20 ↔ 41		By similarity
	Disulfide bond	28 ↔ ?		By similarity
	Disulfide bond	42 ↔ ?		By similarity
Experimental info
	Non-terminal residue	44	1

Sequences

	Sequence		Length	Mass (Da)
	P10846 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 09B844C5530DC73F	FASTA	44	4,797
	10 20 30 40 SGPWMCYPGY AFKVPALPGC RPVLLLQCNG SQVPEAVLRD CCQQ

References

[1]	"New dimeric inhibitor of heterologous alpha-amylases encoded by a duplicated gene in the short arm of chromosome 3B of wheat (Triticum aestivum L.)." Sanchez-Monge R., Gomez L., Garcia-Olmedo F., Salcedo G. Eur. J. Biochem. 183:37-40(1989) [PubMed: 2787745] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: cv. Chinese Spring.
+	Additional computationally mapped references.

Cross-references

3D structure databases
ProteinModelPortal	P10846.
SMR	P10846. Positions 5-44.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P10846.
Family and domain databases
InterPro	IPR006106. Allergen/soft/tryp_amyl_inhib. IPR006105. Allergen/tryp_amyl_inhib_CS. IPR016140. Bifunc_inhib/LTP/seed_store. IPR013771. Trypsin/amylase_inhib. [Graphical view]
Gene3D	G3DSA:1.10.120.10. Trypsin/amylase_inhib. 1 hit.
PRINTS	PR00808. AMLASEINHBTR.
SUPFAM	SSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.
PROSITE	PS00426. CEREAL_TRYP_AMYL_INH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IAA3_WHEAT

Accession

Primary (citable) accession number: P10846

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	August 10, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program