P10835 (MTC3_PBCVC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase CviBIII
Short name=M.CviBIII
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase CviBIII

Gene names

Name:

CVIBIIIM

Organism

Paramecium bursaria Chlorella virus NC1A (PBCV-NC1A)

Taxonomic identifier

46020 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Phycodnaviridae › Chlorovirus

Virus host

Chlorella [TaxID: 3071]

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence TCGA, causes specific methylation on A-4 on both strands.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Modification methylase CviBIII		PRO_0000087948

Sequences

	Sequence		Length	Mass (Da)
	P10835 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: B22D471367D61BBC	FASTA	377	42,895
	10 20 30 40 50 60 MNVEQYTQVT TDFEKTLTKE KKSKQGIFFT PKTVREKLFG FTEHFQNTPG FSILEPSCGT 70 80 90 100 110 120 GEIISECVER FPLASIKGVE LDNDMSTICS KKYAEYNVDI VNEDFLLWKG GKFDFIVGNP 130 140 150 160 170 180 PYVVRPSGYK NDNRIAKGRS NLYVEFLYKC ITEHLKEDGI LAFIIPSTIG NSSFYEPIRK 190 200 210 220 230 240 LIITLDILSF EILDKHDFCD TNTRLCSIVI KNSPGTGKYT YRDYICDKDI PHHGNSYIGS 250 260 270 280 290 300 LDLKFKTGFA WANVNKFFTD KSEIPFFTSS NIKLNEIHIG DKMKYLTQDT TKFFTGKALL 310 320 330 340 350 360 IKTASAGKRG GRFEFGFSLY ENDKWAVDND IIVIQGPDTV LSIVQDVLMK DVTNEFINIL 370 VNNGHISMKL LKSIPLF

References

[1]	"Molecular cloning and characterization of the gene encoding the DNA methyltransferase, M.CviBIII, from Chlorella virus NC-1A." Narva K.E., Wendell D.L., Skrdla M.P., van Etten J.L. Nucleic Acids Res. 15:9807-9823(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X06618 Genomic DNA. Translation: CAA29835.1.
3D structure databases
ProteinModelPortal	P10835.
ModBase	Search...
Protein family/group databases
REBASE	752. M.CviBIII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTC3_PBCVC

Accession

Primary (citable) accession number: P10835

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program