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Protein

Adenosylhomocysteinase

Gene

sahA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.

Catalytic activityi

S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine.

Cofactori

NAD+Note: Binds 1 NAD+ per subunit.

Pathwayi: L-homocysteine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine.
Proteins known to be involved in this subpathway in this organism are:
  1. Adenosylhomocysteinase (sahA)
This subpathway is part of the pathway L-homocysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine, the pathway L-homocysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei56SubstrateBy similarity1
Binding sitei131SubstrateBy similarity1
Binding sitei156SubstrateBy similarity1
Binding sitei186SubstrateBy similarity1
Binding sitei190SubstrateBy similarity1
Binding sitei191NADBy similarity1
Binding sitei243NADBy similarity1
Binding sitei345NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi157 – 159NADBy similarity3
Nucleotide bindingi222 – 227NADBy similarity6
Nucleotide bindingi299 – 301NADBy similarity3

GO - Molecular functioni

  • adenosylhomocysteinase activity Source: dictyBase
  • cAMP binding Source: dictyBase
  • NAD binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processOne-carbon metabolism
LigandNAD

Enzyme and pathway databases

ReactomeiR-DDI-156581 Methylation
R-DDI-1614635 Sulfur amino acid metabolism
UniPathwayiUPA00314; UER00076

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylhomocysteinase (EC:3.3.1.1)
Short name:
AdoHcyase
Alternative name(s):
S-adenosyl-L-homocysteine hydrolase
Gene namesi
Name:sahA
ORF Names:DDB_G0267418
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 1, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0267418 sahA

Subcellular locationi

GO - Cellular componenti

  • actin rod Source: dictyBase
  • cell cortex Source: dictyBase
  • cytoplasm Source: dictyBase
  • cytosol Source: GO_Central
  • nucleus Source: dictyBase
  • phagocytic vesicle Source: dictyBase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001169111 – 431AdenosylhomocysteinaseAdd BLAST431

Proteomic databases

PaxDbiP10819
PRIDEiP10819

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi44689.DDB0191108

Structurei

3D structure databases

ProteinModelPortaliP10819
SMRiP10819
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the adenosylhomocysteinase family.Curated

Phylogenomic databases

eggNOGiKOG1370 Eukaryota
COG0499 LUCA
InParanoidiP10819
KOiK01251
OMAiTGNRDII
PhylomeDBiP10819

Family and domain databases

CDDicd00401 SAHH, 1 hit
HAMAPiMF_00563 AdoHcyase, 1 hit
InterProiView protein in InterPro
IPR034373 Adenosylhomocysteinase
IPR000043 Adenosylhomocysteinase-like
IPR015878 Ado_hCys_hydrolase_NAD-bd
IPR036291 NAD(P)-bd_dom_sf
IPR020082 S-Ado-L-homoCys_hydrolase_CS
PANTHERiPTHR23420 PTHR23420, 1 hit
PfamiView protein in Pfam
PF05221 AdoHcyase, 1 hit
PF00670 AdoHcyase_NAD, 1 hit
PIRSFiPIRSF001109 Ad_hcy_hydrolase, 1 hit
SMARTiView protein in SMART
SM00996 AdoHcyase, 1 hit
SM00997 AdoHcyase_NAD, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00936 ahcY, 1 hit
PROSITEiView protein in PROSITE
PS00738 ADOHCYASE_1, 1 hit
PS00739 ADOHCYASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P10819-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKLHYKVKD ISLAAWGRKE IEIAENEMPG LMTLRKKYGP AQILKGARIA
60 70 80 90 100
GCLHMTIQTA VLIETLTALG AQVQWSSCNI FSTQDQAAAA IAATGVPVYA
110 120 130 140 150
WKGETEEEYN WCVEQTIVFQ DGQPLNMILD DGGDLTNLVH EKYPQFLAGI
160 170 180 190 200
KGISEETTTG VHNLYKMFKE GKLKVPAINV NDSVTKSKFD NLYGCRESLI
210 220 230 240 250
DGIKRATDVM IAGKVAVVAG YGDVGKGCAQ SLSKMGARVL VTEIDPINAL
260 270 280 290 300
QACMDGYQIV TMETAAPLSN IFVTTTGCRD IVRGEHFAVM KEDAIVCNIG
310 320 330 340 350
HFDCEIDVAW LNANAKKDTV KPQVDRYTLA NGVHIILLAE GRLVNLGCGT
360 370 380 390 400
GHPSFVMSNS FCNQTLAQIA LWTKTEEYPL GVHFLPKILD EEVARLHLDQ
410 420 430
LGAKLTTLTE KQSEYLSVPV AGPYKVDHYR Y
Length:431
Mass (Da):47,280
Last modified:December 4, 2007 - v2
Checksum:iC621FC6BE5BCBA2A
GO

Sequence cautioni

The sequence CAA31040 differs from that shown. Reason: Frameshift at position 270.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25E → A in AAA33165 (PubMed:3288206).Curated1
Sequence conflicti123Missing in AAA33165 (PubMed:3288206).Curated1
Sequence conflicti137N → T in AAA33165 (PubMed:3288206).Curated1
Sequence conflicti159T → H in AAA33165 (PubMed:3288206).Curated1
Sequence conflicti313A → G in CAA31040 (PubMed:3139100).Curated1
Sequence conflicti325D → S in CAA31040 (PubMed:3139100).Curated1
Sequence conflicti361 – 365FCNQT → SVTK in CAA31040 (PubMed:3139100).Curated5
Sequence conflicti384F → L in AAA33165 (PubMed:3288206).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19937 mRNA Translation: AAA33165.1
AAFI02000003 Genomic DNA Translation: EAL73161.1
X12523 mRNA Translation: CAA31040.1 Frameshift.
PIRiA27655
RefSeqiXP_647635.1, XM_642543.1

Genome annotation databases

EnsemblProtistsiEAL73161; EAL73161; DDB_G0267418
GeneIDi8616450
KEGGiddi:DDB_G0267418

Similar proteinsi

Entry informationi

Entry nameiSAHH_DICDI
AccessioniPrimary (citable) accession number: P10819
Secondary accession number(s): Q55F98
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: December 4, 2007
Last modified: February 28, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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