P10815 (CG23_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G2/mitotic-specific cyclin cdc13

Gene names

Name:	cdc13
ORF Names:	SPBC582.03

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for the control of the cell cycle at the G2/M (mitosis) transition. Interacts with the cdc2 protein kinase to form MPF. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis. Involved in the reorganization of the cytoskeleton on transition from G2 to mitosis. Association with rum1 promotes its proteolysis during G1. Ref.1 Ref.2 Ref.7 Ref.8 Ref.10
Subunit structure	Interacts with cdc2 and rum1. Associates with microtubules. Also interacts with cdc11. Ref.8 Ref.10 Ref.11 Ref.12 Ref.14
Subcellular location	Nucleus. Cytoplasm › cytoskeleton › spindle pole body Ref.4 Ref.7 Ref.15.
Post-translational modification	Phosphorylated on Ser-177 and/or Ser-180 and/or Ser-183.
Sequence similarities	Belongs to the cyclin family. Cyclin AB subfamily. Contains 1 cyclin N-terminal domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Nucleus
Molecular function	Cyclin
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular protein localization Inferred from mutant phenotype PubMed 16824200. Source: PomBase cellular response to nitrogen starvation Inferred from mutant phenotype PubMed 19366728. Source: PomBase microtubule cytoskeleton organization Inferred from mutant phenotype PubMed 3428262. Source: PomBase mitosis Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of G0 to G1 transition Inferred from mutant phenotype PubMed 19366728. Source: PomBase negative regulation of induction of conjugation with cellular fusion Inferred from mutant phenotype PubMed 18059475. Source: PomBase positive regulation of protein localization to nucleus Inferred from mutant phenotype PubMed 2569363. Source: PomBase regulation of G2/M transition of mitotic cell cycle Inferred from mutant phenotype Ref.2. Source: PomBase regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from direct assay Ref.7. Source: PomBase signal transduction Non-traceable author statement. Source: PomBase
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW mitotic spindle pole body Inferred from direct assay Ref.15. Source: PomBase nucleus Inferred from direct assay Ref.15Ref.7PubMed 2569363. Source: PomBase
Molecular_function	cyclin-dependent protein serine/threonine kinase regulator activity Non-traceable author statement. Source: PomBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
rum1	P40380	5	EBI-1187843,EBI-1187892

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	G2/mitotic-specific cyclin cdc13		PRO_0000080402
Regions
	Domain	206 – 332	127	Cyclin N-terminal
Experimental info
	Sequence conflict	479	1	D → H no nucleotide entry Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P10815 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: B2C267CF57F5FD36	FASTA	482	55,609
	10 20 30 40 50 60 MTTRRLTRQH LLANTLGNND ENHPSNHIAR AKSSLHSSEN SLVNGKKATV SSTNVPKKRH 70 80 90 100 110 120 ALDDVSNFHN KEGVPLASKN TNVRHTTASV STRRALEEKS IIPATDDEPA SKKRRQPSVF 130 140 150 160 170 180 NSSVPSLPQH LSTKSHSVST HGVDAFHKDQ ATIPKKLKKD VDERVVSKDI PKLHRDSVES 190 200 210 220 230 240 PESQDWDDLD AEDWADPLMV SEYVVDIFEY LNELEIETMP SPTYMDRQKE LAWKMRGILT 250 260 270 280 290 300 DWLIEVHSRF RLLPETLFLA VNIIDRFLSL RVCSLNKLQL VGIAALFIAS KYEEVMCPSV 310 320 330 340 350 360 QNFVYMADGG YDEEEILQAE RYILRVLEFN LAYPNPMNFL RRISKADFYD IQTRTVAKYL 370 380 390 400 410 420 VEIGLLDHKL LPYPPSQQCA AAMYLAREML GRGPWNRNLV HYSGYEEYQL ISVVKKMINY 430 440 450 460 470 480 LQKPVQHEAF FKKYASKKFM KASLFVRDWI KKNSIPLGDD ADEDYTFHKQ KRIQHDMKDE EW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Involvement of cdc13+ in mitotic control in Schizosaccharomyces pombe: possible interaction of the gene product with microtubules." Booher R., Beach D. EMBO J. 7:2321-2327(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, ASSOCIATION WITH MICROTUBULES.
[2]	"Cloning and sequencing of the cyclin-related cdc13+ gene and a cytological study of its role in fission yeast mitosis." Hagan I., Hayles J., Nurse P. J. Cell Sci. 91:587-595(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[3]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[4]	"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 22-226, SUBCELLULAR LOCATION. Strain: ATCC 38364 / 968.
[5]	"Cyclin in fission yeast." Solomon M., Booher R., Kirschner M., Beach B. Cell 54:738-739(1988) [PubMed] [Europe PMC] [Abstract] Cited for: SIMILARITY TO THE CYCLIN FAMILY.
[6]	"Cyclin in fission yeast." Goebl M., Bryers B. Cell 54:739-740(1988) Cited for: SIMILARITY TO THE CYCLIN FAMILY.
[7]	"Fission yeast cyclin: subcellular localisation and cell cycle regulation." Alfa C.E., Booher R., Beach D., Hyams J.S. J. Cell Sci. Suppl. 12:9-19(1989) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"Two fission yeast B-type cyclins, cig2 and Cdc13, have different functions in mitosis." Bueno A., Russell P. Mol. Cell. Biol. 13:2286-2297(1993) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CDC2. Strain: 972 / ATCC 24843.
[9]	"A quantitative model for the cdc2 control of S phase and mitosis in fission yeast." Stern B., Nurse P. Trends Genet. 12:345-350(1996) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW ON ASSOCIATION WITH CDC2.
[10]	"p25rum1 promotes proteolysis of the mitotic B-cyclin p56cdc13 during G1 of the fission yeast cell cycle." Correa-Bordes J., Gulli M.P., Nurse P. EMBO J. 16:4657-4664(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH RUM1.
[11]	"The Cdk inhibitors p25rum1 and p40SIC1 are functional homologues that play similar roles in the regulation of the cell cycle in fission and budding yeast." Sanchez-Diaz A., Gonzalez I., Arellano M., Moreno S. J. Cell Sci. 111:843-851(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RUM1.
[12]	"Cyclin B proteolysis and the cyclin-dependent kinase inhibitor rum1p are required for pheromone-induced G1 arrest in fission yeast." Stern B., Nurse P. Mol. Biol. Cell 9:1309-1321(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RUM1.
[13]	"Shotgun identification of protein modifications from protein complexes and lens tissue." MacCoss M.J., McDonald W.H., Saraf A., Sadygov R., Clark J.M., Tasto J.J., Gould K.L., Wolters D., Washburn M., Weiss A., Clark J.I., Yates J.R. III Proc. Natl. Acad. Sci. U.S.A. 99:7900-7905(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SERINE RESIDUES, MASS SPECTROMETRY.
[14]	"Sid4p-Cdc11p assembles the septation initiation network and its regulators at the S. pombe SPB." Morrell J.L., Tomlin G.C., Rajagopalan S., Venkatram S., Feoktistova A.S., Tasto J.J., Mehta S., Jennings J.L., Link A., Balasubramanian M.K., Gould K.L. Curr. Biol. 14:579-584(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CDC11.
[15]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X12557 Genomic DNA. Translation: CAA31070.1. CU329671 Genomic DNA. Translation: CAB46666.1. AB027869 Genomic DNA. Translation: BAA87173.1.
PIR	A34948. S01153.
RefSeq	NP_595171.1. NM_001021079.2.
3D structure databases
ProteinModelPortal	P10815.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-620N.
IntAct	P10815. 4 interactions.
MINT	MINT-4687197.
STRING	4896.SPBC582.03-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC582.03.1; SPBC582.03.1:pep; SPBC582.03.
GeneID	2540880.
KEGG	spo:SPBC582.03.
Organism-specific databases
PomBase	SPBC582.03.
Phylogenomic databases
eggNOG	COG5024.
HOGENOM	HOG000167672.
KO	K02220.
OMA	SILACAC.
OrthoDB	EOG4X9BRK.
Gene expression databases
GermOnline	SPBC582.03. Schizosaccharomyces pombe.
Family and domain databases
Gene3D	1.10.472.10. 2 hits.
InterPro	IPR013763. Cyclin-like. IPR014400. Cyclin_A/B/D/E. IPR004367. Cyclin_C-dom. IPR006671. Cyclin_N. [Graphical view]
Pfam	PF02984. Cyclin_C. 1 hit. PF00134. Cyclin_N. 1 hit. [Graphical view]
PIRSF	PIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMART	SM00385. CYCLIN. 2 hits. [Graphical view]
SUPFAM	SSF47954. Cyclin_like. 2 hits.
PROSITE	PS00292. CYCLINS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801996.

Entry information

Entry name

CG23_SCHPO

Accession

Primary (citable) accession number: P10815
Secondary accession number(s): Q9UU16

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 29, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program