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P10810 (CD14_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Monocyte differentiation antigen CD14
Alternative name(s):
Myeloid cell-specific leucine-rich glycoprotein
CD_antigen=CD14
Gene names
Name:Cd14
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules By similarity.

Subunit structure

Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4 By similarity.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor.

Sequence similarities

Contains 11 LRR (leucine-rich) repeats.

Ontologies

Keywords
   Biological processImmunity
Inflammatory response
Innate immunity
   Cellular componentCell membrane
Membrane
   DomainLeucine-rich repeat
Repeat
Signal
   PTMDisulfide bond
Glycoprotein
GPI-anchor
Lipoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to lipopolysaccharide

Inferred from electronic annotation. Source: Ensembl

cellular response to lipoteichoic acid

Inferred from electronic annotation. Source: Ensembl

inflammatory response

Inferred from electronic annotation. Source: UniProtKB-KW

innate immune response

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of cytokine secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of endocytosis

Inferred from mutant phenotype PubMed 22078883. Source: UniProtKB

positive regulation of tumor necrosis factor production

Inferred from electronic annotation. Source: Ensembl

response to ethanol

Inferred from electronic annotation. Source: Ensembl

response to heat

Inferred from electronic annotation. Source: Ensembl

response to magnesium ion

Inferred from electronic annotation. Source: Ensembl

response to molecule of bacterial origin

Inferred from direct assay PubMed 15493995. Source: UniProtKB

response to tumor necrosis factor

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentanchored component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

cell surface

Inferred from electronic annotation. Source: Ensembl

extracellular space

Inferred from electronic annotation. Source: Ensembl

membrane raft

Inferred from direct assay PubMed 15493995. Source: UniProtKB

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionlipopolysaccharide binding

Inferred from electronic annotation. Source: Ensembl

lipoteichoic acid binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1515
Chain16 – 336321Monocyte differentiation antigen CD14
PRO_0000020887
Propeptide337 – 36630Removed in mature form Potential
PRO_0000020888

Regions

Repeat50 – 7829LRR 1
Repeat79 – 11436LRR 2
Repeat115 – 14026LRR 3
Repeat141 – 16828LRR 4
Repeat169 – 19224LRR 5
Repeat193 – 22028LRR 6
Repeat221 – 24727LRR 7
Repeat248 – 27225LRR 8
Repeat273 – 29321LRR 9
Repeat294 – 31522LRR 10
Repeat316 – 34025LRR 11
Region16 – 8267LPS-binding
Region38 – 7841Ligand-binding pocket rim

Amino acid modifications

Lipidation3361GPI-anchor amidated asparagine Potential
Glycosylation331N-linked (GlcNAc...) Potential
Glycosylation1471N-linked (GlcNAc...) Ref.5
Glycosylation1801N-linked (GlcNAc...) Ref.5
Glycosylation2761N-linked (GlcNAc...) Ref.5
Glycosylation3171N-linked (GlcNAc...) Potential
Disulfide bond23 ↔ 32 Ref.5
Disulfide bond30 ↔ 47 Ref.5
Disulfide bond183 ↔ 213 Ref.5
Disulfide bond237 ↔ 266 Ref.5

Secondary structure

................................................. 366
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P10810 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 57C4492EC7EA3AA1

FASTA36639,204
        10         20         30         40         50         60 
MERVLGLLLL LLVHASPAPP EPCELDEESC SCNFSDPKPD WSSAFNCLGA ADVELYGGGR 

        70         80         90        100        110        120 
SLEYLLKRVD TEADLGQFTD IIKSLSLKRL TVRAARIPSR ILFGALRVLG ISGLQELTLE 

       130        140        150        160        170        180 
NLEVTGTAPP PLLEATGPDL NILNLRNVSW ATRDAWLAEL QQWLKPGLKV LSIAQAHSLN 

       190        200        210        220        230        240 
FSCEQVRVFP ALSTLDLSDN PELGERGLIS ALCPLKFPTL QVLALRNAGM ETPSGVCSAL 

       250        260        270        280        290        300 
AAARVQLQGL DLSHNSLRDA AGAPSCDWPS QLNSLNLSFT GLKQVPKGLP AKLSVLDLSY 

       310        320        330        340        350        360 
NRLDRNPSPD ELPQVGNLSL KGNPFLDSES HSEKFNSGVV TAGAPSSQAV ALSGTLALLL 


GDRLFV 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide and amino acid sequences of the mouse CD14 gene."
Miyazaki Y., Setoguchi M., Yoshida S., Higuchi Y., Akizuki S., Yamamoto S.
Nucleic Acids Res. 17:2132-2132(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BALB/c.
Tissue: Liver.
[2]"Mouse and human CD14 (myeloid cell-specific leucine-rich glycoprotein) primary structure deduced from cDNA clones."
Setoguchi M., Nasu N., Yoshida S., Higuchi Y., Akizuki S., Yamamoto S.
Biochim. Biophys. Acta 1008:213-222(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ICR.
Tissue: Macrophage.
[3]"CD14 is a member of the family of leucine-rich proteins and is encoded by a gene syntenic with multiple receptor genes."
Ferrero E., Hsieh C.L., Francke U., Goyert S.M.
J. Immunol. 145:331-336(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NMRI.
Tissue: Mammary gland.
[5]"Crystal structure of CD14 and its implications for lipopolysaccharide signaling."
Kim J.I., Lee C.J., Jin M.S., Lee C.H., Paik S.G., Lee H., Lee J.O.
J. Biol. Chem. 280:11347-11351(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 20-329, DISULFIDE BONDS, GLYCOSYLATION AT ASN-147; ASN-180 AND ASN-276, DOMAINS LEUCINE-RICH REPEATS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X13987 Genomic DNA. Translation: CAA32166.1.
X13333 mRNA. Translation: CAA31710.1.
M34510 Genomic DNA. Translation: AAA37387.1.
BC057889 mRNA. Translation: AAH57889.1.
PIRTDMSM4. S03605.
RefSeqNP_033971.1. NM_009841.3.
UniGeneMm.3460.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1WWLX-ray2.50A/B20-329[»]
ProteinModelPortalP10810.
SMRP10810. Positions 22-329.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198573. 1 interaction.

PTM databases

PhosphoSiteP10810.

Proteomic databases

PaxDbP10810.
PRIDEP10810.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000061829; ENSMUSP00000056669; ENSMUSG00000051439.
GeneID12475.
KEGGmmu:12475.
UCSCuc008eof.1. mouse.

Organism-specific databases

CTD929.
MGIMGI:88318. Cd14.

Phylogenomic databases

eggNOGNOG86091.
HOGENOMHOG000237268.
HOVERGENHBG005269.
InParanoidP10810.
KOK04391.
OMALCPHKFP.
OrthoDBEOG751NFQ.
PhylomeDBP10810.
TreeFamTF338550.

Gene expression databases

ArrayExpressP10810.
BgeeP10810.
CleanExMM_CD14.
GenevestigatorP10810.

Family and domain databases

InterProIPR001611. Leu-rich_rpt.
IPR016337. Monocyte_diff_Ag_CD14.
[Graphical view]
PIRSFPIRSF002017. CD14. 1 hit.
PROSITEPS51450. LRR. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP10810.
NextBio281354.
PMAP-CutDBP10810.
PROP10810.
SOURCESearch...

Entry information

Entry nameCD14_MOUSE
AccessionPrimary (citable) accession number: P10810
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot