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P10810

- CD14_MOUSE

UniProt

P10810 - CD14_MOUSE

Protein

Monocyte differentiation antigen CD14

Gene

Cd14

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules By similarity.By similarity

    GO - Molecular functioni

    1. lipopolysaccharide binding Source: Ensembl
    2. lipoteichoic acid binding Source: Ensembl

    GO - Biological processi

    1. cellular response to lipopolysaccharide Source: Ensembl
    2. cellular response to lipoteichoic acid Source: Ensembl
    3. inflammatory response Source: UniProtKB-KW
    4. innate immune response Source: UniProtKB-KW
    5. positive regulation of cytokine secretion Source: Ensembl
    6. positive regulation of endocytosis Source: UniProtKB
    7. positive regulation of tumor necrosis factor production Source: Ensembl
    8. response to ethanol Source: Ensembl
    9. response to heat Source: Ensembl
    10. response to magnesium ion Source: Ensembl
    11. response to molecule of bacterial origin Source: UniProtKB
    12. response to tumor necrosis factor Source: Ensembl

    Keywords - Biological processi

    Immunity, Inflammatory response, Innate immunity

    Enzyme and pathway databases

    ReactomeiREACT_196636. TRIF-mediated programmed cell death.
    REACT_198539. TRAF6 mediated induction of TAK1 complex.
    REACT_225463. Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon.
    REACT_226192. IKK complex recruitment mediated by RIP1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Monocyte differentiation antigen CD14
    Alternative name(s):
    Myeloid cell-specific leucine-rich glycoprotein
    CD_antigen: CD14
    Gene namesi
    Name:Cd14
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:88318. Cd14.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. cell surface Source: Ensembl
    3. extracellular space Source: Ensembl
    4. membrane raft Source: UniProtKB
    5. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1515Add
    BLAST
    Chaini16 – 336321Monocyte differentiation antigen CD14PRO_0000020887Add
    BLAST
    Propeptidei337 – 36630Removed in mature formSequence AnalysisPRO_0000020888Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi23 ↔ 321 Publication
    Disulfide bondi30 ↔ 471 Publication
    Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi147 – 1471N-linked (GlcNAc...)1 Publication
    Glycosylationi180 – 1801N-linked (GlcNAc...)1 Publication
    Disulfide bondi183 ↔ 2131 Publication
    Disulfide bondi237 ↔ 2661 Publication
    Glycosylationi276 – 2761N-linked (GlcNAc...)1 Publication
    Glycosylationi317 – 3171N-linked (GlcNAc...)Sequence Analysis
    Lipidationi336 – 3361GPI-anchor amidated asparagineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiP10810.
    PRIDEiP10810.

    PTM databases

    PhosphoSiteiP10810.

    Miscellaneous databases

    PMAP-CutDBP10810.

    Expressioni

    Gene expression databases

    ArrayExpressiP10810.
    BgeeiP10810.
    CleanExiMM_CD14.
    GenevestigatoriP10810.

    Interactioni

    Subunit structurei

    Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4.By similarity

    Protein-protein interaction databases

    BioGridi198573. 1 interaction.

    Structurei

    Secondary structure

    1
    366
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi36 – 383
    Helixi41 – 466
    Beta strandi47 – 493
    Beta strandi51 – 6111
    Helixi65 – 684
    Helixi76 – 849
    Beta strandi89 – 968
    Helixi99 – 10911
    Beta strandi116 – 1238
    Beta strandi132 – 1343
    Beta strandi141 – 1477
    Beta strandi151 – 1555
    Helixi156 – 1616
    Beta strandi170 – 1756
    Turni183 – 1853
    Beta strandi194 – 1963
    Helixi204 – 2118
    Beta strandi222 – 2243
    Helixi233 – 24210
    Beta strandi248 – 2514
    Beta strandi274 – 2763
    Beta strandi291 – 2977
    Turni309 – 3113
    Beta strandi314 – 3196
    Turni324 – 3263

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1WWLX-ray2.50A/B20-329[»]
    ProteinModelPortaliP10810.
    SMRiP10810. Positions 22-329.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP10810.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati50 – 7829LRR 1Add
    BLAST
    Repeati79 – 11436LRR 2Add
    BLAST
    Repeati115 – 14026LRR 3Add
    BLAST
    Repeati141 – 16828LRR 4Add
    BLAST
    Repeati169 – 19224LRR 5Add
    BLAST
    Repeati193 – 22028LRR 6Add
    BLAST
    Repeati221 – 24727LRR 7Add
    BLAST
    Repeati248 – 27225LRR 8Add
    BLAST
    Repeati273 – 29321LRR 9Add
    BLAST
    Repeati294 – 31522LRR 10Add
    BLAST
    Repeati316 – 34025LRR 11Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni16 – 8267LPS-bindingAdd
    BLAST
    Regioni38 – 7841Ligand-binding pocket rimAdd
    BLAST

    Sequence similaritiesi

    Contains 11 LRR (leucine-rich) repeats.Curated

    Keywords - Domaini

    Leucine-rich repeat, Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG86091.
    HOGENOMiHOG000237268.
    HOVERGENiHBG005269.
    InParanoidiP10810.
    KOiK04391.
    OMAiWATGGAW.
    OrthoDBiEOG751NFQ.
    PhylomeDBiP10810.
    TreeFamiTF338550.

    Family and domain databases

    InterProiIPR001611. Leu-rich_rpt.
    IPR016337. Monocyte_diff_Ag_CD14.
    [Graphical view]
    PIRSFiPIRSF002017. CD14. 1 hit.
    PROSITEiPS51450. LRR. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10810-1 [UniParc]FASTAAdd to Basket

    « Hide

    MERVLGLLLL LLVHASPAPP EPCELDEESC SCNFSDPKPD WSSAFNCLGA    50
    ADVELYGGGR SLEYLLKRVD TEADLGQFTD IIKSLSLKRL TVRAARIPSR 100
    ILFGALRVLG ISGLQELTLE NLEVTGTAPP PLLEATGPDL NILNLRNVSW 150
    ATRDAWLAEL QQWLKPGLKV LSIAQAHSLN FSCEQVRVFP ALSTLDLSDN 200
    PELGERGLIS ALCPLKFPTL QVLALRNAGM ETPSGVCSAL AAARVQLQGL 250
    DLSHNSLRDA AGAPSCDWPS QLNSLNLSFT GLKQVPKGLP AKLSVLDLSY 300
    NRLDRNPSPD ELPQVGNLSL KGNPFLDSES HSEKFNSGVV TAGAPSSQAV 350
    ALSGTLALLL GDRLFV 366
    Length:366
    Mass (Da):39,204
    Last modified:July 1, 1989 - v1
    Checksum:i57C4492EC7EA3AA1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13987 Genomic DNA. Translation: CAA32166.1.
    X13333 mRNA. Translation: CAA31710.1.
    M34510 Genomic DNA. Translation: AAA37387.1.
    BC057889 mRNA. Translation: AAH57889.1.
    CCDSiCCDS29159.1.
    PIRiS03605. TDMSM4.
    RefSeqiNP_033971.1. NM_009841.3.
    UniGeneiMm.3460.

    Genome annotation databases

    EnsembliENSMUST00000061829; ENSMUSP00000056669; ENSMUSG00000051439.
    GeneIDi12475.
    KEGGimmu:12475.
    UCSCiuc008eof.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13987 Genomic DNA. Translation: CAA32166.1 .
    X13333 mRNA. Translation: CAA31710.1 .
    M34510 Genomic DNA. Translation: AAA37387.1 .
    BC057889 mRNA. Translation: AAH57889.1 .
    CCDSi CCDS29159.1.
    PIRi S03605. TDMSM4.
    RefSeqi NP_033971.1. NM_009841.3.
    UniGenei Mm.3460.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1WWL X-ray 2.50 A/B 20-329 [» ]
    ProteinModelPortali P10810.
    SMRi P10810. Positions 22-329.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198573. 1 interaction.

    Chemistry

    ChEMBLi CHEMBL3038514.

    PTM databases

    PhosphoSitei P10810.

    Proteomic databases

    PaxDbi P10810.
    PRIDEi P10810.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000061829 ; ENSMUSP00000056669 ; ENSMUSG00000051439 .
    GeneIDi 12475.
    KEGGi mmu:12475.
    UCSCi uc008eof.1. mouse.

    Organism-specific databases

    CTDi 929.
    MGIi MGI:88318. Cd14.

    Phylogenomic databases

    eggNOGi NOG86091.
    HOGENOMi HOG000237268.
    HOVERGENi HBG005269.
    InParanoidi P10810.
    KOi K04391.
    OMAi WATGGAW.
    OrthoDBi EOG751NFQ.
    PhylomeDBi P10810.
    TreeFami TF338550.

    Enzyme and pathway databases

    Reactomei REACT_196636. TRIF-mediated programmed cell death.
    REACT_198539. TRAF6 mediated induction of TAK1 complex.
    REACT_225463. Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon.
    REACT_226192. IKK complex recruitment mediated by RIP1.

    Miscellaneous databases

    EvolutionaryTracei P10810.
    NextBioi 281354.
    PMAP-CutDB P10810.
    PROi P10810.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P10810.
    Bgeei P10810.
    CleanExi MM_CD14.
    Genevestigatori P10810.

    Family and domain databases

    InterProi IPR001611. Leu-rich_rpt.
    IPR016337. Monocyte_diff_Ag_CD14.
    [Graphical view ]
    PIRSFi PIRSF002017. CD14. 1 hit.
    PROSITEi PS51450. LRR. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BALB/c.
      Tissue: Liver.
    2. "Mouse and human CD14 (myeloid cell-specific leucine-rich glycoprotein) primary structure deduced from cDNA clones."
      Setoguchi M., Nasu N., Yoshida S., Higuchi Y., Akizuki S., Yamamoto S.
      Biochim. Biophys. Acta 1008:213-222(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ICR.
      Tissue: Macrophage.
    3. "CD14 is a member of the family of leucine-rich proteins and is encoded by a gene syntenic with multiple receptor genes."
      Ferrero E., Hsieh C.L., Francke U., Goyert S.M.
      J. Immunol. 145:331-336(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: NMRI.
      Tissue: Mammary gland.
    5. "Crystal structure of CD14 and its implications for lipopolysaccharide signaling."
      Kim J.I., Lee C.J., Jin M.S., Lee C.H., Paik S.G., Lee H., Lee J.O.
      J. Biol. Chem. 280:11347-11351(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 20-329, DISULFIDE BONDS, GLYCOSYLATION AT ASN-147; ASN-180 AND ASN-276, DOMAINS LEUCINE-RICH REPEATS.

    Entry informationi

    Entry nameiCD14_MOUSE
    AccessioniPrimary (citable) accession number: P10810
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3