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Protein

Monocyte differentiation antigen CD14

Gene

Cd14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:16148141). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135, PubMed:15895089). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (By similarity). Acts as an accessory receptor for M.tuberculosis lipoproteins LprA, LprG and LpqH, in conjunction with coreceptors TLR2 and TLR1. The lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712). Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (By similarity).By similarity5 Publications

GO - Molecular functioni

GO - Biological processi

  • cellular response to diacyl bacterial lipopeptide Source: UniProtKB
  • cellular response to lipopolysaccharide Source: UniProtKB
  • cellular response to lipoteichoic acid Source: MGI
  • cellular response to triacyl bacterial lipopeptide Source: UniProtKB
  • inflammatory response Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • lipopolysaccharide-mediated signaling pathway Source: UniProtKB
  • positive regulation of cytokine secretion Source: Ensembl
  • positive regulation of endocytosis Source: UniProtKB
  • positive regulation of interferon-gamma production Source: UniProtKB
  • positive regulation of tumor necrosis factor production Source: UniProtKB
  • positive regulation of type I interferon production Source: CACAO
  • receptor-mediated endocytosis Source: CACAO
  • response to bacterium Source: UniProtKB
  • response to electrical stimulus Source: Ensembl
  • response to ethanol Source: Ensembl
  • response to heat Source: Ensembl
  • response to magnesium ion Source: Ensembl
  • response to molecule of bacterial origin Source: UniProtKB
  • response to tumor necrosis factor Source: Ensembl
  • toll-like receptor 4 signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiR-MMU-166016. Toll Like Receptor 4 (TLR4) Cascade.
R-MMU-166020. Transfer of LPS from LBP carrier to CD14.
R-MMU-166166. MyD88-independent TLR3/TLR4 cascade.
R-MMU-5686938. Regulation of TLR by endogenous ligand.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Monocyte differentiation antigen CD14
Alternative name(s):
Myeloid cell-specific leucine-rich glycoprotein
CD_antigen: CD14
Gene namesi
Name:Cd14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:88318. Cd14.

Subcellular locationi

  • Cell membrane 2 Publications; Lipid-anchorGPI-anchor By similarity
  • Secreted By similarity
  • Membrane raft By similarity
  • Golgi apparatus By similarity

  • Note: Soluble, secreted forms seem to exist. They may arise by cleavage of the GPI anchor.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Mice are fertile and appear healthy when kept in a clean, microbe-free environment. Mice do not respond to bacterial smooth lipopolysaccharide (LPS). Contrary to wild-type, they do not develop toxic shock or secrete TNF in response to LPS. Surprisingly, they have fewer live bacteria in their lungs and bloodstream after inoculation with bacteria and are not killed by an inoculum that is lethal to wild-type; they are killed when the inoculum is further increased.1 Publication

Chemistry databases

ChEMBLiCHEMBL2384896.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Add BLAST15
ChainiPRO_000002088716 – 336Monocyte differentiation antigen CD14Add BLAST321
PropeptideiPRO_0000020888337 – 366Removed in mature formSequence analysisAdd BLAST30

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi23 ↔ 321 Publication
Disulfide bondi30 ↔ 471 Publication
Glycosylationi33N-linked (GlcNAc...)Sequence analysis1
Glycosylationi147N-linked (GlcNAc...)1 Publication1
Glycosylationi180N-linked (GlcNAc...)1 Publication1
Disulfide bondi183 ↔ 2131 Publication
Disulfide bondi237 ↔ 2661 Publication
Glycosylationi276N-linked (GlcNAc...)1 Publication1
Glycosylationi317N-linked (GlcNAc...)Sequence analysis1
Lipidationi336GPI-anchor amidated asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP10810.
PeptideAtlasiP10810.
PRIDEiP10810.

PTM databases

iPTMnetiP10810.
PhosphoSitePlusiP10810.
SwissPalmiP10810.

Miscellaneous databases

PMAP-CutDBP10810.

Expressioni

Tissue specificityi

Detected on peritoneal macrophages (at protein level) (PubMed:8612135). Cell surface expression detected in lung alveolar macrophages, dendritic macrophages and lung macrophages (at protein level) (PubMed:19362712).2 Publications

Gene expression databases

BgeeiENSMUSG00000051439.
CleanExiMM_CD14.
ExpressionAtlasiP10810. baseline and differential.
GenevisibleiP10810. MM.

Interactioni

Subunit structurei

Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4 (By similarity). Interacts with LPS-bound LPB. Interacts with LPAR1 (PubMed:21821728). Interacts with the TLR2:TLR6 or TLR2:TLR1 heterodimers; upon interaction with ligands such as diacylated lipopeptides and triacylated lipopeptides, respectively. Interacts with MYO18A (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi198573. 1 interactor.
STRINGi10090.ENSMUSP00000056669.

Structurei

Secondary structure

1366
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi36 – 38Combined sources3
Helixi41 – 46Combined sources6
Beta strandi47 – 49Combined sources3
Beta strandi51 – 61Combined sources11
Helixi65 – 68Combined sources4
Helixi76 – 84Combined sources9
Beta strandi89 – 96Combined sources8
Helixi99 – 109Combined sources11
Beta strandi116 – 123Combined sources8
Beta strandi132 – 134Combined sources3
Beta strandi141 – 147Combined sources7
Beta strandi151 – 155Combined sources5
Helixi156 – 161Combined sources6
Beta strandi170 – 175Combined sources6
Turni183 – 185Combined sources3
Beta strandi194 – 196Combined sources3
Helixi204 – 211Combined sources8
Beta strandi222 – 224Combined sources3
Helixi233 – 242Combined sources10
Beta strandi248 – 251Combined sources4
Beta strandi274 – 276Combined sources3
Beta strandi291 – 297Combined sources7
Turni309 – 311Combined sources3
Beta strandi314 – 319Combined sources6
Turni324 – 326Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WWLX-ray2.50A/B20-329[»]
ProteinModelPortaliP10810.
SMRiP10810.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10810.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati50 – 78LRR 1Add BLAST29
Repeati79 – 114LRR 2Add BLAST36
Repeati115 – 140LRR 3Add BLAST26
Repeati141 – 168LRR 4Add BLAST28
Repeati169 – 192LRR 5Add BLAST24
Repeati193 – 220LRR 6Add BLAST28
Repeati221 – 247LRR 7Add BLAST27
Repeati248 – 272LRR 8Add BLAST25
Repeati273 – 293LRR 9Add BLAST21
Repeati294 – 315LRR 10Add BLAST22
Repeati316 – 340LRR 11Add BLAST25

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni284 – 366Required for response to bacterial lipopolysaccharide (LPS)1 PublicationAdd BLAST83

Domaini

The C-terminal leucine-rich repeat (LRR) region is required for responses to smooth LPS.1 Publication

Sequence similaritiesi

Contains 11 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IXG9. Eukaryota.
ENOG41118N2. LUCA.
GeneTreeiENSGT00390000005689.
HOGENOMiHOG000237268.
HOVERGENiHBG005269.
InParanoidiP10810.
KOiK04391.
OMAiLCPHKFP.
OrthoDBiEOG091G0DA3.
PhylomeDBiP10810.
TreeFamiTF338550.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR016337. Monocyte_diff_Ag_CD14.
[Graphical view]
PIRSFiPIRSF002017. CD14. 1 hit.
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10810-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERVLGLLLL LLVHASPAPP EPCELDEESC SCNFSDPKPD WSSAFNCLGA
60 70 80 90 100
ADVELYGGGR SLEYLLKRVD TEADLGQFTD IIKSLSLKRL TVRAARIPSR
110 120 130 140 150
ILFGALRVLG ISGLQELTLE NLEVTGTAPP PLLEATGPDL NILNLRNVSW
160 170 180 190 200
ATRDAWLAEL QQWLKPGLKV LSIAQAHSLN FSCEQVRVFP ALSTLDLSDN
210 220 230 240 250
PELGERGLIS ALCPLKFPTL QVLALRNAGM ETPSGVCSAL AAARVQLQGL
260 270 280 290 300
DLSHNSLRDA AGAPSCDWPS QLNSLNLSFT GLKQVPKGLP AKLSVLDLSY
310 320 330 340 350
NRLDRNPSPD ELPQVGNLSL KGNPFLDSES HSEKFNSGVV TAGAPSSQAV
360
ALSGTLALLL GDRLFV
Length:366
Mass (Da):39,204
Last modified:July 1, 1989 - v1
Checksum:i57C4492EC7EA3AA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13987 Genomic DNA. Translation: CAA32166.1.
X13333 mRNA. Translation: CAA31710.1.
M34510 Genomic DNA. Translation: AAA37387.1.
BC057889 mRNA. Translation: AAH57889.1.
CCDSiCCDS29159.1.
PIRiS03605. TDMSM4.
RefSeqiNP_033971.1. NM_009841.4.
UniGeneiMm.3460.

Genome annotation databases

EnsembliENSMUST00000061829; ENSMUSP00000056669; ENSMUSG00000051439.
GeneIDi12475.
KEGGimmu:12475.
UCSCiuc008eof.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13987 Genomic DNA. Translation: CAA32166.1.
X13333 mRNA. Translation: CAA31710.1.
M34510 Genomic DNA. Translation: AAA37387.1.
BC057889 mRNA. Translation: AAH57889.1.
CCDSiCCDS29159.1.
PIRiS03605. TDMSM4.
RefSeqiNP_033971.1. NM_009841.4.
UniGeneiMm.3460.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WWLX-ray2.50A/B20-329[»]
ProteinModelPortaliP10810.
SMRiP10810.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198573. 1 interactor.
STRINGi10090.ENSMUSP00000056669.

Chemistry databases

ChEMBLiCHEMBL2384896.

PTM databases

iPTMnetiP10810.
PhosphoSitePlusiP10810.
SwissPalmiP10810.

Proteomic databases

PaxDbiP10810.
PeptideAtlasiP10810.
PRIDEiP10810.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000061829; ENSMUSP00000056669; ENSMUSG00000051439.
GeneIDi12475.
KEGGimmu:12475.
UCSCiuc008eof.2. mouse.

Organism-specific databases

CTDi929.
MGIiMGI:88318. Cd14.

Phylogenomic databases

eggNOGiENOG410IXG9. Eukaryota.
ENOG41118N2. LUCA.
GeneTreeiENSGT00390000005689.
HOGENOMiHOG000237268.
HOVERGENiHBG005269.
InParanoidiP10810.
KOiK04391.
OMAiLCPHKFP.
OrthoDBiEOG091G0DA3.
PhylomeDBiP10810.
TreeFamiTF338550.

Enzyme and pathway databases

ReactomeiR-MMU-166016. Toll Like Receptor 4 (TLR4) Cascade.
R-MMU-166020. Transfer of LPS from LBP carrier to CD14.
R-MMU-166166. MyD88-independent TLR3/TLR4 cascade.
R-MMU-5686938. Regulation of TLR by endogenous ligand.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

EvolutionaryTraceiP10810.
PMAP-CutDBP10810.
PROiP10810.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000051439.
CleanExiMM_CD14.
ExpressionAtlasiP10810. baseline and differential.
GenevisibleiP10810. MM.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR016337. Monocyte_diff_Ag_CD14.
[Graphical view]
PIRSFiPIRSF002017. CD14. 1 hit.
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD14_MOUSE
AccessioniPrimary (citable) accession number: P10810
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 30, 2016
This is version 162 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.