Reviewed, UniProtKB/Swiss-Prot P10807 (ADH_DROLE)

Names and origin

Protein names

Recommended name:
    Alcohol dehydrogenase
    EC=1.1.1.1

Gene names

Name:

Adh

Organism

Drosophila lebanonensis (Fruit fly) (Scaptodrosophila lebanonensis)

Taxonomic identifier

7225 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Scaptodrosophila

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	An alcohol + NAD+ = an aldehyde or ketone + NADH.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	alcohol metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alcohol dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 254

254

Alcohol dehydrogenase

PRO_0000054471

Regions

Nucleotide binding

10 – 33

24

NAD

Sites

Active site

151

1

Proton acceptor

Binding site

138

1

Substrate

Amino acid modifications

Modified residue

1

1

N-acetylmethionine Ref.1

Secondary structure

1

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254

Helix Strand Turn

Details...

Beta strand

7 – 11

5

Turn

12 – 14

3

Helix

16 – 27

12

Beta strand

31 – 39

9

Helix

42 – 51

10

Beta strand

55 – 61

7

Helix

68 – 82

15

Beta strand

87 – 90

4

Helix

100 – 107

8

Helix

109 – 122

14

Helix

124 – 126

3

Beta strand

131 – 136

6

Helix

139 – 141

3

Helix

149 – 172

24

Beta strand

173 – 181

9

Beta strand

183 – 186

4

Beta strand

189 – 191

3

Helix

195 – 197

3

Helix

202 – 206

5

Helix

214 – 227

14

Beta strand

233 – 237

5

Beta strand

240 – 243

4

Sequences

	Sequence		Length	Mass (Da)
	P10807-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 56DA3644E6B9CDCE	FASTA	254	27,792
	10 20 30 40 50 60 MDLTNKNVIF VAALGGIGLD TSRELVKRNL KNFVILDRVE NPTALAELKA INPKVNITFH 70 80 90 100 110 120 TYDVTVPVAE SKKLLKKIFD QLKTVDILIN GAGILDDHQI ERTIAINFTG LVNTTTAILD 130 140 150 160 170 180 FWDKRKGGPG GIIANICSVT GFNAIHQVPV YSASKAAVVS FTNSLAKLAP ITGVTAYSIN 190 200 210 220 230 240 PGITRTPLVH TFNSWLDVEP RVAELLLSHP TQTSEQCGQN FVKAIEANKN GAIWKLDLGT 250 LEAIEWTKHW DSHI

References

[1]	"The primary structure of alcohol dehydrogenase from Drosophila lebanonensis. Extensive variation within insect 'short-chain' alcohol dehydrogenase lacking zinc." Villarroya A., Juan E., Egestad B., Joernvall H. Eur. J. Biochem. 180:191-197(1989) [PubMed: 2707261] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Nucleotide sequence of the Adh gene of Drosophila lebanonensis." Juan E., Papaceit M., Quintana A. Nucleic Acids Res. 18:6420-6420(1990) [PubMed: 2243785] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 323G.
[3]	"Nucleotide sequence of the Adh gene of Drosophila lebanonensis." Albalat R., Gonzalez-Duarte R. Nucleic Acids Res. 18:6706-6706(1990) [PubMed: 2251140] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	Erratum Albalat R., Gonzalez-Duarte R. Nucleic Acids Res. 19:424-424(1991) [PubMed: 1849634] [Abstract]
[5]	"Adh and Adh-dup sequences of Drosophila lebanonensis and D. immigrans: interspecies comparisons." Albalat R., Gonzalez-Duarte R. Gene 126:171-178(1993) [PubMed: 8482531] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"ADH and phylogenetic relationships of Drosophila lebanonesis (Scaptodrosophila)." Villarroya A., Juan E. J. Mol. Evol. 32:421-428(1991) [PubMed: 1904097] [Abstract] Cited for: PHYLOGENETIC RELATIONSHIP TO OTHER DROSOPHILA ADH.
[7]	"The refined crystal structure of Drosophila lebanonensis alcohol dehydrogenase at 1.9-A resolution." Benach J., Atrian S., Gonzalez-Duarte R., Ladenstein R. J. Mol. Biol. 282:383-399(1998) [PubMed: 9735295] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS).
[8]	"The catalytic reaction and inhibition mechanism of Drosophila alcohol dehydrogenase: observation of an enzyme-bound NAD-ketone adduct at 1.4-A resolution by X-ray crystallography." Benach J., Atrian S., Gonzalez-Duarte R., Ladenstein R. J. Mol. Biol. 289:335-355(1999) [PubMed: 10366509] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) IN COMPLEX WITH NAD AND PRODUCT.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X53429 Genomic DNA. Translation: CAA37520.1. Sequence problems.
X54814 Genomic DNA. Translation: CAA38583.1.
M97637 Genomic DNA. Translation: AAA28355.1.

PIR

DEFFRL. S12695.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A4U	X-ray	1.92	A/B	1-254	[»]
1B14	X-ray	2.40	A/B	1-254	[»]
1B15	X-ray	2.20	A/B	1-254	[»]
1B16	X-ray	1.40	A/B	1-254	[»]
1B2L	X-ray	1.60	A	1-254	[»]
1SBY	X-ray	1.10	A/B	1-254	[»]

ModBase

Search...

Organism-specific databases

FlyBase

FBgn0012438. Dleb\Adh.

Enzyme and pathway databases

BRENDA

1.1.1.1. 189313.

Family and domain databases

InterPro

IPR002198. DH_sc/Rdtase_SDR.
IPR002425. Insect_ADH.
IPR002424. Insect_AlcDH_fam.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

PANTHER

PTHR19410. ADH_short_C2. 1 hit.

Pfam

PF00106. adh_short. 1 hit.
[Graphical view]

PRINTS

PR01168. ALCDHDRGNASE.
PR01167. INSADHFAMILY.
PR00080. SDRFAMILY.

PROSITE

PS00061. ADH_SHORT. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ADH_DROLE

Accession

Primary (citable) accession number: P10807

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	September 22, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project