Reviewed, UniProtKB/Swiss-Prot P10798 (RBS3B_ARATH)

Names and origin

Protein names

Recommended name:
    Ribulose bisphosphate carboxylase small chain 3B, chloroplastic
      Short name=RuBisCO small subunit 3B
    EC=4.1.1.39

Gene names

Name:	RBCS-3B
Synonyms:	ATS3B
Ordered Locus Names:	At5g38410
ORF Names:	MXI10.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity.
Catalytic activity	2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O. 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O(2).
Subunit structure	8 large chains + 8 small chains.
Subcellular location	Plastid › chloroplast.
Miscellaneous	There are four genes coding for RBS in Arabidopsis thaliana.
Sequence similarities	Belongs to the RuBisCO small chain family.

Ontologies

Keywords
Biological process	Calvin cycle Carbon dioxide fixation Photorespiration Photosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Molecular function	Lyase Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photorespiration Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast ribulose bisphosphate carboxylase complex Inferred from electronic annotation. Source: InterPro
Molecular function	monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct ribulose-bisphosphate carboxylase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
COI1	O04197	2	EBI-639640,EBI-401159

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Notes: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P10798-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 55	55	Chloroplast
	Chain	56 – 181	126	Ribulose bisphosphate carboxylase small chain 3B, chloroplastic		PRO_0000031466
Experimental info
	Sequence conflict	47	1	A → T in CAA32702. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 9A5688A9D055254D	FASTA	181	20,284
	10 20 30 40 50 60 MASSMLSSAA VVTSPAQATM VAPFTGLKSS AAFPVTRKTN KDITSIASNG GRVSCMKVWP 70 80 90 100 110 120 PIGKKKFETL SYLPDLSDVE LAKEVDYLLR NKWIPCVEFE LEHGFVYREH GNTPGYYDGR 130 140 150 160 170 180 YWTMWKLPLF GCTDSAQVLK EVEECKKEYP GAFIRIIGFD NTRQVQCISF IAYKPPSFTE A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Four genes in two diverged subfamilies encode the ribulose-1,5-bisphosphate carboxylase small subunit polypeptides of Arabidopsis thaliana." Krebbers E., Seurinck J., Herdies L., Cashmore A.R., Timko M.P. Plant Mol. Biol. 11:745-759(1988) [Agricola: IND91035191] Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Columbia K85.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones." Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S. DNA Res. 4:215-230(1997) [PubMed: 9330910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X14564 Genomic DNA. Translation: CAA32702.1. AB005248 Genomic DNA. Translation: BAB09353.1. AF360124 mRNA. Translation: AAK25834.1. AY054552 mRNA. Translation: AAK96743.1. AY051025 mRNA. Translation: AAK93702.1. AF410314 mRNA. Translation: AAK95300.1. AF462822 mRNA. Translation: AAL58912.1. AY064686 mRNA. Translation: AAL47390.1. AY098970 mRNA. Translation: AAM19980.1. BT000721 mRNA. Translation: AAN31863.1.
PIR	RKMUB3. S03719.
RefSeq	NP_198657.1.
UniGene	At.24772 At.71421
3D structure databases
SMR	P10798. Positions 56-178.
ModBase	Search...
Protein-protein interaction databases
IntAct	P10798.
2-D gel databases
SWISS-2DPAGE	P99057.
Genome annotation databases
GeneID	833828.
GenomeReviews	Gene locus AT5G38410 in contig BA000015_GR.
NMPDR	fig|3702.1.peg.25533.
Organism-specific databases
TAIR	At5g38410.
Gene expression databases
ArrayExpress	P10798.
Family and domain databases
InterPro	IPR000894. RuBisCO_sc. [Graphical view]
Gene3D	G3DSA:3.30.190.10. RuBisCO_small. 1 hit.
Pfam	PF00101. RuBisCO_small. 1 hit. [Graphical view]
PRINTS	PR00152. RUBISCOSMALL.
ProDom	PD000290. RuBisCO_small. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

RBS3B_ARATH

Accession

Primary (citable) accession number: P10798
Secondary accession number(s): Q9FF21

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2002
Last modified:	November 25, 2008
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)