P10798 (RBS3B_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribulose bisphosphate carboxylase small chain 3B, chloroplastic
Short name=RuBisCO small subunit 3B
EC=4.1.1.39

Gene names

Name:	RBCS-3B
Synonyms:	ATS3B
Ordered Locus Names:	At5g38410
ORF Names:	MXI10.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity.
Catalytic activity	2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.
Subunit structure	8 large chains + 8 small chains.
Subcellular location	Plastid › chloroplast.
Miscellaneous	There are four genes coding for RBS in Arabidopsis thaliana.
Sequence similarities	Belongs to the RuBisCO small chain family.

Ontologies

Keywords
Biological process	Calvin cycle Carbon dioxide fixation Photorespiration Photosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Molecular function	Lyase Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	photorespiration Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW ribulose-bisphosphate carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P10798-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 55	55	Chloroplast
	Chain	56 – 181	126	Ribulose bisphosphate carboxylase small chain 3B, chloroplastic		PRO_0000031466
Experimental info
	Sequence conflict	47	1	A → T in CAA32702. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 9A5688A9D055254D	FASTA	181	20,284
	10 20 30 40 50 60 MASSMLSSAA VVTSPAQATM VAPFTGLKSS AAFPVTRKTN KDITSIASNG GRVSCMKVWP 70 80 90 100 110 120 PIGKKKFETL SYLPDLSDVE LAKEVDYLLR NKWIPCVEFE LEHGFVYREH GNTPGYYDGR 130 140 150 160 170 180 YWTMWKLPLF GCTDSAQVLK EVEECKKEYP GAFIRIIGFD NTRQVQCISF IAYKPPSFTE A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Four genes in two diverged subfamilies encode the ribulose-1,5-bisphosphate carboxylase small subunit polypeptides of Arabidopsis thaliana." Krebbers E., Seurinck J., Herdies L., Cashmore A.R., Timko M.P. Plant Mol. Biol. 11:745-759(1988) [AGRICOLA] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Columbia K85.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones." Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S. DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14564 Genomic DNA. Translation: CAA32702.1. AB005248 Genomic DNA. Translation: BAB09353.1. CP002688 Genomic DNA. Translation: AED94311.1. AF360124 mRNA. Translation: AAK25834.1. AY054552 mRNA. Translation: AAK96743.1. AY051025 mRNA. Translation: AAK93702.1. AF410314 mRNA. Translation: AAK95300.1. AF462822 mRNA. Translation: AAL58912.1. AY064686 mRNA. Translation: AAL47390.1. AY098970 mRNA. Translation: AAM19980.1. BT000721 mRNA. Translation: AAN31863.1.
IPI	IPI00545353.
PIR	RKMUB3. S03719.
RefSeq	NP_198657.1. NM_123202.3.
UniGene	At.20381. At.24772. At.46639. At.49098. At.49366. At.70032. At.70053. At.71313. At.74604. At.75410. At.75674.
3D structure databases
ProteinModelPortal	P10798.
SMR	P10798. Positions 56-178.
ModBase	Search...
Protein-protein interaction databases
IntAct	P10798. 5 interactions.
2D gel databases
SWISS-2DPAGE	P99057.
Proteomic databases
PaxDb	P10798.
PRIDE	P10798.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G38410.1; AT5G38410.1; AT5G38410.
GeneID	833828.
KEGG	ath:AT5G38410.
Organism-specific databases
TAIR	At5g38410.
Phylogenomic databases
eggNOG	COG4451.
HOGENOM	HOG000141332.
InParanoid	P10798.
KO	K01602.
PhylomeDB	P10798.
ProtClustDB	PLN02289.
Enzyme and pathway databases
BioCyc	MetaCyc:AT5G38410-MONOMER.
Gene expression databases
Genevestigator	P10798.
Family and domain databases
Gene3D	3.30.190.10. 1 hit.
InterPro	IPR024681. RuBisCO_sc. IPR000894. RuBisCO_sc_dom. IPR024680. RuBisCO_ssu_N. [Graphical view]
Pfam	PF12338. RbcS. 1 hit. PF00101. RuBisCO_small. 1 hit. [Graphical view]
PRINTS	PR00152. RUBISCOSMALL.
SMART	SM00961. RuBisCO_small. 1 hit. [Graphical view]
SUPFAM	SSF55239. RuBisCO_small. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RBS3B_ARATH

Accession

Primary (citable) accession number: P10798
Secondary accession number(s): Q9FF21

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2002
Last modified:	May 1, 2013
This is version 125 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program