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P10796 (RBS1B_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribulose bisphosphate carboxylase small chain 1B, chloroplastic

Short name=RuBisCO small subunit 1B
EC=4.1.1.39
Gene names
Name:RBCS-1B
Synonyms:ATS1B
Ordered Locus Names:At5g38430
ORF Names:MXI10.15
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length181 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity.

Catalytic activity

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.

3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.

Subunit structure

8 large chains + 8 small chains.

Subcellular location

Plastidchloroplast membrane; Peripheral membrane protein. Plastidchloroplast stroma Ref.5.

Miscellaneous

There are four genes coding for RBS in Arabidopsis thaliana.

Sequence similarities

Belongs to the RuBisCO small chain family.

Ontologies

Keywords
   Biological processCalvin cycle
Carbon dioxide fixation
Photorespiration
Photosynthesis
   Cellular componentChloroplast
Membrane
Plastid
   DomainTransit peptide
   Molecular functionLyase
Monooxygenase
Oxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processphotorespiration

Inferred from electronic annotation. Source: UniProtKB-KW

reductive pentose-phosphate cycle

Inferred from electronic annotation. Source: UniProtKB-KW

response to blue light

Inferred from expression pattern PubMed 18820083. Source: TAIR

response to far red light

Inferred from expression pattern PubMed 18820083. Source: TAIR

response to red light

Inferred from expression pattern PubMed 18820083. Source: TAIR

   Cellular_componentapoplast

Inferred from direct assay PubMed 18538804. Source: TAIR

chloroplast

Inferred from direct assay PubMed 15028209PubMed 18431481. Source: TAIR

chloroplast envelope

Inferred from direct assay Ref.5PubMed 12938931PubMed 20061580. Source: TAIR

chloroplast membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

chloroplast stroma

Inferred from direct assay PubMed 16207701PubMed 18633119PubMed 20061580. Source: TAIR

membrane

Inferred from direct assay PubMed 17432890. Source: TAIR

thylakoid

Inferred from direct assay PubMed 16648217. Source: TAIR

   Molecular_functionmonooxygenase activity

Inferred from electronic annotation. Source: UniProtKB-KW

ribulose-bisphosphate carboxylase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5555Chloroplast
Chain56 – 181126Ribulose bisphosphate carboxylase small chain 1B, chloroplastic
PRO_0000031464

Sequences

Sequence LengthMass (Da)Tools
P10796 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 63D5E3BA93E0B6CA

FASTA18120,286
        10         20         30         40         50         60 
MASSMLSSAA VVTSPAQATM VAPFTGLKSS ASFPVTRKAN NDITSITSNG GRVSCMKVWP 

        70         80         90        100        110        120 
PIGKKKFETL SYLPDLTDVE LAKEVDYLLR NKWIPCVEFE LEHGFVYREH GNTPGYYDGR 

       130        140        150        160        170        180 
YWTMWKLPLF GCTDSAQVLK EVEECKKEYP GAFIRIIGFD NTRQVQCISF IAYKPPSFTD 


A 

« Hide

References

« Hide 'large scale' references
[1]"Four genes in two diverged subfamilies encode the ribulose-1,5-bisphosphate carboxylase small subunit polypeptides of Arabidopsis thaliana."
Krebbers E., Seurinck J., Herdies L., Cashmore A.R., Timko M.P.
Plant Mol. Biol. 11:745-759(1988) [AGRICOLA] [Europe PMC]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: cv. Columbia K85.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana."
Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N.
Mol. Cell. Proteomics 2:325-345(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Wassilewskija.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14564 Genomic DNA. Translation: CAA32700.1.
AB005248 Genomic DNA. Translation: BAB09355.1.
CP002688 Genomic DNA. Translation: AED94315.1.
AF410283 mRNA. Translation: AAK95269.1.
AY143814 mRNA. Translation: AAN28753.1.
PIRRKMUB1. S03717.
RefSeqNP_198659.1. NM_123204.3.
UniGeneAt.25361.
At.25577.
At.39706.
At.46639.
At.49098.
At.70032.
At.70053.
At.73308.

3D structure databases

ProteinModelPortalP10796.
SMRP10796. Positions 56-178.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid19081. 6 interactions.
IntActP10796. 3 interactions.
STRING3702.AT5G38430.1-P.

2D gel databases

World-2DPAGE0003:P10796.

Proteomic databases

PaxDbP10796.
PRIDEP10796.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G38430.1; AT5G38430.1; AT5G38430.
GeneID833830.
KEGGath:AT5G38430.

Organism-specific databases

TAIRAT5G38430.

Phylogenomic databases

eggNOGCOG4451.
HOGENOMHOG000141332.
InParanoidP10796.
KOK01602.
OMAAPTVMAS.
PhylomeDBP10796.
ProtClustDBPLN02289.

Enzyme and pathway databases

BioCycMetaCyc:AT5G38430-MONOMER.

Gene expression databases

GenevestigatorP10796.

Family and domain databases

Gene3D3.30.190.10. 1 hit.
InterProIPR024681. RuBisCO_sc.
IPR000894. RuBisCO_sc_dom.
IPR024680. RuBisCO_ssu_N.
[Graphical view]
PfamPF12338. RbcS. 1 hit.
PF00101. RuBisCO_small. 1 hit.
[Graphical view]
PRINTSPR00152. RUBISCOSMALL.
SMARTSM00961. RuBisCO_small. 1 hit.
[Graphical view]
SUPFAMSSF55239. SSF55239. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRBS1B_ARATH
AccessionPrimary (citable) accession number: P10796
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names