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Protein

Ribulose bisphosphate carboxylase small chain 1A, chloroplastic

Gene

RBCS-1A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site (By similarity).By similarity

Miscellaneous

There are four genes coding for RBS in Arabidopsis thaliana.

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.

GO - Molecular functioni

GO - Biological processi

  • chloroplast ribulose bisphosphate carboxylase complex biogenesis Source: TAIR
  • photorespiration Source: UniProtKB-KW
  • photosynthesis Source: TAIR
  • reductive pentose-phosphate cycle Source: UniProtKB-KW
  • response to blue light Source: TAIR
  • response to cold Source: TAIR
  • response to far red light Source: TAIR
  • response to red light Source: TAIR

Keywordsi

Molecular functionLyase, Monooxygenase, Oxidoreductase
Biological processCalvin cycle, Carbon dioxide fixation, Photorespiration, Photosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:AT1G67090-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase small chain 1A, chloroplastic (EC:4.1.1.39)
Short name:
RuBisCO small subunit 1A
Gene namesi
Name:RBCS-1A
Synonyms:ATS1A
Ordered Locus Names:At1g67090
ORF Names:F1O19.14, F5A8.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G67090.
TAIRilocus:2033686. AT1G67090.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 55ChloroplastAdd BLAST55
ChainiPRO_000003146356 – 180Ribulose bisphosphate carboxylase small chain 1A, chloroplasticAdd BLAST125

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei113PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP10795.
PRIDEiP10795.

2D gel databases

World-2DPAGEi0003:P10795.

PTM databases

iPTMnetiP10795.

Expressioni

Gene expression databases

ExpressionAtlasiP10795. baseline and differential.
GenevisibleiP10795. AT.

Interactioni

Subunit structurei

8 large chains + 8 small chains.

Protein-protein interaction databases

BioGridi28250. 9 interactors.
IntActiP10795. 6 interactors.
MINTiMINT-2584041.
STRINGi3702.AT1G67090.1.

Structurei

3D structure databases

ProteinModelPortaliP10795.
SMRiP10795.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuBisCO small chain family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IM2H. Eukaryota.
COG4451. LUCA.
HOGENOMiHOG000141332.
InParanoidiP10795.
KOiK01602.
OMAiYREHGNS.
OrthoDBiEOG09360OM1.
PhylomeDBiP10795.

Family and domain databases

Gene3Di3.30.190.10. 1 hit.
InterProiView protein in InterPro
IPR024681. RuBisCO_sc.
IPR000894. RuBisCO_sc_dom.
IPR024680. RuBisCO_ssu_N.
IPR036385. RuBisCO_ssu_sf.
PfamiView protein in Pfam
PF12338. RbcS. 1 hit.
PF00101. RuBisCO_small. 1 hit.
PRINTSiPR00152. RUBISCOSMALL.
SMARTiView protein in SMART
SM00961. RuBisCO_small. 1 hit.
SUPFAMiSSF55239. SSF55239. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P10795-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSMLSSAT MVASPAQATM VAPFNGLKSS AAFPATRKAN NDITSITSNG
60 70 80 90 100
GRVNCMQVWP PIGKKKFETL SYLPDLTDSE LAKEVDYLIR NKWIPCVEFE
110 120 130 140 150
LEHGFVYREH GNSPGYYDGR YWTMWKLPLF GCTDSAQVLK EVEECKKEYP
160 170 180
NAFIRIIGFD NTRQVQCISF IAYKPPSFTG
Length:180
Mass (Da):20,216
Last modified:January 10, 2003 - v2
Checksum:i5DE768676D61F8F7
GO

Sequence cautioni

The sequence AAK49590 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA31948 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti115G → R in AAG40356 (PubMed:14593172).Curated1
Sequence conflicti171I → V in CAA31948 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13611 Genomic DNA. Translation: CAA31948.1. Sequence problems.
AC004146 Genomic DNA. Translation: AAD10655.1.
AC007152 Genomic DNA. No translation available.
CP002684 Genomic DNA. Translation: AEE34594.1.
AF325011 mRNA. Translation: AAG40363.1.
AF325004 mRNA. Translation: AAG40356.1.
AF372874 mRNA. Translation: AAK49590.1. Different initiation.
AF410291 mRNA. Translation: AAK95277.1.
AY054188 mRNA. Translation: AAL06849.1.
AY054581 mRNA. Translation: AAK96772.1.
AY058831 mRNA. Translation: AAL24219.1.
AY059940 mRNA. Translation: AAL24422.1.
AY062612 mRNA. Translation: AAL32690.1.
AY062711 mRNA. Translation: AAL32789.1.
AY065101 mRNA. Translation: AAL38277.1.
AY093380 mRNA. Translation: AAM13379.1.
AY093388 mRNA. Translation: AAM13387.1.
AY097366 mRNA. Translation: AAM19882.1.
BT000362 mRNA. Translation: AAN15681.1.
BT002076 mRNA. Translation: AAN72087.1.
X68342 Other DNA. Translation: CAA48415.1. Different termination.
PIRiG96694.
S03720. RKMUA1.
RefSeqiNP_176880.1. NM_105379.4. [P10795-1]
UniGeneiAt.12721.
At.30194.
At.37531.
At.46639.
At.49098.
At.67454.
At.68122.
At.70032.
At.70053.

Genome annotation databases

EnsemblPlantsiAT1G67090.1; AT1G67090.1; AT1G67090. [P10795-1]
GeneIDi843029.
GrameneiAT1G67090.1; AT1G67090.1; AT1G67090.
KEGGiath:AT1G67090.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRBS1A_ARATH
AccessioniPrimary (citable) accession number: P10795
Secondary accession number(s): Q94JW2, Q9FPI6, Q9SAV4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 10, 2003
Last modified: October 25, 2017
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families