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Protein

Ribulose bisphosphate carboxylase small chain 1A, chloroplastic

Gene

RBCS-1A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site (By similarity).By similarity

Miscellaneous

There are four genes coding for RBS in Arabidopsis thaliana.

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.

GO - Molecular functioni

GO - Biological processi

  • chloroplast ribulose bisphosphate carboxylase complex biogenesis Source: TAIR
  • photorespiration Source: UniProtKB-KW
  • photosynthesis Source: TAIR
  • reductive pentose-phosphate cycle Source: UniProtKB-KW
  • response to blue light Source: TAIR
  • response to cold Source: TAIR
  • response to far red light Source: TAIR
  • response to red light Source: TAIR

Keywordsi

Molecular functionLyase, Monooxygenase, Oxidoreductase
Biological processCalvin cycle, Carbon dioxide fixation, Photorespiration, Photosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:AT1G67090-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase small chain 1A, chloroplastic (EC:4.1.1.39)
Short name:
RuBisCO small subunit 1A
Gene namesi
Name:RBCS-1A
Synonyms:ATS1A
Ordered Locus Names:At1g67090
ORF Names:F1O19.14, F5A8.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G67090
TAIRilocus:2033686 AT1G67090

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 55ChloroplastSequence analysisAdd BLAST55
ChainiPRO_000003146356 – 180Ribulose bisphosphate carboxylase small chain 1A, chloroplasticAdd BLAST125

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei113PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP10795
PRIDEiP10795

2D gel databases

World-2DPAGEi0003:P10795

PTM databases

iPTMnetiP10795

Expressioni

Gene expression databases

ExpressionAtlasiP10795 baseline and differential
GenevisibleiP10795 AT

Interactioni

Subunit structurei

8 large chains + 8 small chains.

Protein-protein interaction databases

BioGridi282509 interactors.
IntActiP10795 6 interactors.
MINTiP10795
STRINGi3702.AT1G67090.1

Structurei

3D structure databases

ProteinModelPortaliP10795
SMRiP10795
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuBisCO small chain family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IM2H Eukaryota
COG4451 LUCA
HOGENOMiHOG000141332
InParanoidiP10795
KOiK01602
OMAiVAQASMV
OrthoDBiEOG09360OM1
PhylomeDBiP10795

Family and domain databases

Gene3Di3.30.190.101 hit
InterProiView protein in InterPro
IPR024681 RuBisCO_sc
IPR000894 RuBisCO_sc_dom
IPR024680 RuBisCO_ssu_N
IPR036385 RuBisCO_ssu_sf
PfamiView protein in Pfam
PF12338 RbcS, 1 hit
PF00101 RuBisCO_small, 1 hit
PRINTSiPR00152 RUBISCOSMALL
SMARTiView protein in SMART
SM00961 RuBisCO_small, 1 hit
SUPFAMiSSF55239 SSF55239, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P10795-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSMLSSAT MVASPAQATM VAPFNGLKSS AAFPATRKAN NDITSITSNG
60 70 80 90 100
GRVNCMQVWP PIGKKKFETL SYLPDLTDSE LAKEVDYLIR NKWIPCVEFE
110 120 130 140 150
LEHGFVYREH GNSPGYYDGR YWTMWKLPLF GCTDSAQVLK EVEECKKEYP
160 170 180
NAFIRIIGFD NTRQVQCISF IAYKPPSFTG
Length:180
Mass (Da):20,216
Last modified:January 10, 2003 - v2
Checksum:i5DE768676D61F8F7
GO

Sequence cautioni

The sequence AAK49590 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA31948 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti115G → R in AAG40356 (PubMed:14593172).Curated1
Sequence conflicti171I → V in CAA31948 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13611 Genomic DNA Translation: CAA31948.1 Sequence problems.
AC004146 Genomic DNA Translation: AAD10655.1
AC007152 Genomic DNA No translation available.
CP002684 Genomic DNA Translation: AEE34594.1
AF325011 mRNA Translation: AAG40363.1
AF325004 mRNA Translation: AAG40356.1
AF372874 mRNA Translation: AAK49590.1 Different initiation.
AF410291 mRNA Translation: AAK95277.1
AY054188 mRNA Translation: AAL06849.1
AY054581 mRNA Translation: AAK96772.1
AY058831 mRNA Translation: AAL24219.1
AY059940 mRNA Translation: AAL24422.1
AY062612 mRNA Translation: AAL32690.1
AY062711 mRNA Translation: AAL32789.1
AY065101 mRNA Translation: AAL38277.1
AY093380 mRNA Translation: AAM13379.1
AY093388 mRNA Translation: AAM13387.1
AY097366 mRNA Translation: AAM19882.1
BT000362 mRNA Translation: AAN15681.1
BT002076 mRNA Translation: AAN72087.1
AK226468 mRNA Translation: BAE98610.1
AK226776 mRNA Translation: BAE98874.1
AK226546 mRNA Translation: BAE98685.1
AK226565 mRNA Translation: BAE98695.1
X68342 Other DNA Translation: CAA48415.1 Different termination.
PIRiG96694
S03720 RKMUA1
RefSeqiNP_176880.1, NM_105379.4 [P10795-1]
UniGeneiAt.12721
At.30194
At.37531
At.46639
At.49098
At.67454
At.68122
At.70032
At.70053

Genome annotation databases

EnsemblPlantsiAT1G67090.1; AT1G67090.1; AT1G67090 [P10795-1]
GeneIDi843029
GrameneiAT1G67090.1; AT1G67090.1; AT1G67090 [P10795-1]
KEGGiath:AT1G67090

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRBS1A_ARATH
AccessioniPrimary (citable) accession number: P10795
Secondary accession number(s): Q0WVH4
, Q94JW2, Q9FPI6, Q9SAV4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 10, 2003
Last modified: April 25, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome