P10793 (PSAC_PEA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Photosystem I iron-sulfur center
EC=1.97.1.12

Alternative name(s):
9 kDa polypeptide
PSI-C
Photosystem I subunit VII
PsaC

Gene names

Name:	psaC
Synonyms:	frxA

Encoded on

Plastid; Chloroplast

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	81 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Apoprotein for the two 4Fe-4S centers FA and FB of photosystem I (PSI); essential for photochemical activity. FB is the terminal electron acceptor of PSI, donating electrons to ferredoxin. The C-terminus interacts with PsaA/B/D and helps assemble the protein into the PSI complex. Required for binding of PsaD and PsaE to PSI. PSI is a plastocyanin-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn By similarity. HAMAP-Rule MF_01303
Catalytic activity	Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin. HAMAP-Rule MF_01303
Cofactor	Binds 2 4Fe-4S clusters. Cluster 2 is most probably the spectroscopically characterized electron acceptor FA and cluster 1 is most probably FB By similarity.
Subunit structure	The eukaryotic PSI reaction center is composed of at least 11 subunits By similarity.
Subcellular location	Plastid › chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side By similarity HAMAP-Rule MF_01303.
Sequence similarities	Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Photosynthesis Transport
Cellular component	Chloroplast Membrane Photosystem I Plastid Thylakoid
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	photosynthetic electron transport in photosystem I Inferred from electronic annotation. Source: InterPro
Cellular_component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell photosystem I Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2

Chain

2 – 81

80

Photosystem I iron-sulfur center HAMAP-Rule MF_01303

PRO_0000061996

Regions

Domain

2 – 31

30

4Fe-4S ferredoxin-type 1

Domain

39 – 68

30

4Fe-4S ferredoxin-type 2

Sites

Metal binding

11

1

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

14

1

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

17

1

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

21

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

48

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

51

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

54

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

58

1

Iron-sulfur 1 (4Fe-4S) By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

81

Helix Strand Turn

Details...

Beta strand

15 – 17

3

Turn

22 – 24

3

Turn

35 – 37

3

Turn

54 – 56

3

Helix

75 – 77

3

Sequences

	Sequence		Length	Mass (Da)
	P10793 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 680556D8CB5603BF	FASTA	81	8,980
	10 20 30 40 50 60 MSHSVKIYDT CIGCTQCVRA CPTDVLEMIP WGGCKAKQIA SAPRTEDCVG CKRCESACPT 70 80 DFLSVRVYLW HETTRSMGLA Y

References

[1]	"Localization and nucleotide sequence of the gene for the 8 kDa subunit of photosystem I in pea and wheat chloroplast DNA." Dunn P.P.J., Gray J.C. Plant Mol. Biol. 11:311-319(1988) [AGRICOLA] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"N-terminal amino acid sequence analysis of the subunits of pea photosystem I." Dunn P.P.J., Packman L.C., Pappin D., Gray J.C. FEBS Lett. 228:157-161(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-49.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X13157 Genomic DNA. Translation: CAA31554.1.

PIR

FEPM1S. S04033.

RefSeq

YP_003587533.1. NC_014057.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YO9	model	-	C	2-81	[»]
2O01	X-ray	3.40	C	2-81	[»]
2WSC	X-ray	3.30	C	1-81	[»]
2WSE	X-ray	3.49	C	1-81	[»]
2WSF	X-ray	3.48	C	1-81	[»]
3LW5	X-ray	3.30	C	1-81	[»]

ProteinModelPortal

P10793.

SMR

P10793. Positions 2-81.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

9073063.

Phylogenomic databases

ProtClustDB

CHL00065.

Family and domain databases

HAMAP

MF_01303. PSI_PsaC.

InterPro

IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR017491. PSI_PsaC.
[Graphical view]

Pfam

PF13187. Fer4_9. 1 hit.
[Graphical view]

TIGRFAMs

TIGR03048. PS_I_psaC. 1 hit.

PROSITE

PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P10793.

Entry information

Entry name

PSAC_PEA

Accession

Primary (citable) accession number: P10793

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 99 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program