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Protein

S-formylglutathione hydrolase

Gene

ESD

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine hydrolase involved in the detoxification of formaldehyde.2 Publications

Catalytic activityi

S-formylglutathione + H2O = glutathione + formate.1 Publication
4-methylumbelliferyl acetate + H2O = 4-methylumbelliferone + acetate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei149Charge relay system1 Publication1
Active sitei226Charge relay system1 Publication1
Active sitei260Charge relay system1 Publication1

GO - Molecular functioni

  • carboxylic ester hydrolase activity Source: UniProtKB
  • hydrolase activity, acting on ester bonds Source: UniProtKB
  • identical protein binding Source: IntAct
  • methylumbelliferyl-acetate deacetylase activity Source: UniProtKB-EC
  • S-formylglutathione hydrolase activity Source: Reactome

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Serine esterase

Enzyme and pathway databases

ReactomeiR-HSA-156590 Glutathione conjugation

Protein family/group databases

ESTHERihuman-ESD A85-EsteraseD-FGH
MEROPSiS09.990

Names & Taxonomyi

Protein namesi
Recommended name:
S-formylglutathione hydrolase (EC:3.1.2.12)
Short name:
FGH
Alternative name(s):
Esterase D
Methylumbelliferyl-acetate deacetylase (EC:3.1.1.56)
Gene namesi
Name:ESD
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

EuPathDBiHostDB:ENSG00000139684.13
HGNCiHGNC:3465 ESD
MIMi133280 gene
neXtProtiNX_P10768

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi54L → A: 83% of wild-type activity. 1 Publication1
Mutagenesisi149S → A: Loss of activity. 1 Publication1
Mutagenesisi149S → T: 1.3% of wild-type activity. 1 Publication1
Mutagenesisi150M → A: 62% increase in activity. 1 Publication1
Mutagenesisi226D → A: 4.3% of wild-type activity. 1 Publication1
Mutagenesisi226D → N: 9% of wild-type activity. 1 Publication1
Mutagenesisi260H → A: 3% of wild-type activity. 1 Publication1
Mutagenesisi260H → Q: 1.3% of wild-type activity. 1 Publication1

Organism-specific databases

DisGeNETi2098
OpenTargetsiENSG00000139684
PharmGKBiPA27882

Chemistry databases

ChEMBLiCHEMBL2189130
DrugBankiDB00143 Glutathione

Polymorphism and mutation databases

BioMutaiESD
DMDMi544254

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002103392 – 282S-formylglutathione hydrolaseAdd BLAST281

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei4N6-succinyllysineBy similarity1
Modified residuei200N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP10768
MaxQBiP10768
PaxDbiP10768
PeptideAtlasiP10768
PRIDEiP10768
TopDownProteomicsiP10768

2D gel databases

DOSAC-COBS-2DPAGEP10768
OGPiP10768
REPRODUCTION-2DPAGEIPI00411706
UCD-2DPAGEP10768

PTM databases

iPTMnetiP10768
PhosphoSitePlusiP10768
SwissPalmiP10768

Expressioni

Gene expression databases

BgeeiENSG00000139684
CleanExiHS_ESD
ExpressionAtlasiP10768 baseline and differential
GenevisibleiP10768 HS

Organism-specific databases

HPAiHPA039700

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi108402, 31 interactors
IntActiP10768, 9 interactors
STRINGi9606.ENSP00000367992

Structurei

Secondary structure

1282
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 11Combined sources8
Beta strandi14 – 23Combined sources10
Turni24 – 27Combined sources4
Beta strandi28 – 36Combined sources9
Helixi38 – 41Combined sources4
Beta strandi45 – 51Combined sources7
Helixi59 – 64Combined sources6
Helixi68 – 74Combined sources7
Beta strandi77 – 81Combined sources5
Beta strandi93 – 95Combined sources3
Helixi114 – 117Combined sources4
Helixi120 – 125Combined sources6
Helixi127 – 135Combined sources9
Beta strandi136 – 148Combined sources13
Helixi150 – 160Combined sources11
Turni163 – 165Combined sources3
Beta strandi169 – 173Combined sources5
Helixi178 – 180Combined sources3
Helixi182 – 192Combined sources11
Helixi199 – 202Combined sources4
Helixi204 – 208Combined sources5
Beta strandi218 – 223Combined sources6
Helixi227 – 230Combined sources4
Helixi236 – 245Combined sources10
Beta strandi250 – 255Combined sources6
Helixi262 – 279Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FCXX-ray1.50A/B1-282[»]
ProteinModelPortaliP10768
SMRiP10768
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10768

Family & Domainsi

Sequence similaritiesi

Belongs to the esterase D family.Curated

Phylogenomic databases

eggNOGiKOG3101 Eukaryota
COG0627 LUCA
GeneTreeiENSGT00390000011864
HOGENOMiHOG000263929
HOVERGENiHBG001326
InParanoidiP10768
KOiK01070
OMAiEDHLRHH
OrthoDBiEOG091G0FIA
PhylomeDBiP10768
TreeFamiTF300793

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000801 Esterase_put
IPR014186 S-formylglutathione_hydrol
PANTHERiPTHR10061 PTHR10061, 1 hit
PfamiView protein in Pfam
PF00756 Esterase, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
TIGRFAMsiTIGR02821 fghA_ester_D, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10768-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKQISSNK CFGGLQKVFE HDSVELNCKM KFAVYLPPKA ETGKCPALYW
60 70 80 90 100
LSGLTCTEQN FISKSGYHQS ASEHGLVVIA PDTSPRGCNI KGEDESWDFG
110 120 130 140 150
TGAGFYVDAT EDPWKTNYRM YSYVTEELPQ LINANFPVDP QRMSIFGHSM
160 170 180 190 200
GGHGALICAL KNPGKYKSVS AFAPICNPVL CPWGKKAFSG YLGTDQSKWK
210 220 230 240 250
AYDATHLVKS YPGSQLDILI DQGKDDQFLL DGQLLPDNFI AACTEKKIPV
260 270 280
VFRLQEGYDH SYYFIATFIT DHIRHHAKYL NA
Length:282
Mass (Da):31,463
Last modified:June 1, 1994 - v2
Checksum:iBFC20D5FA2BB0DCE
GO

Sequence cautioni

The sequence CAI12225 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI12226 differs from that shown. Reason: Erroneous gene model prediction.Curated

Polymorphismi

There are two major electrophoretic isotypes. The sequence of the ESD*1 variant is shown (PubMed:12721789, PubMed:7907313).2 Publications

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_005202190G → E in allele ESD*2. 2 PublicationsCorresponds to variant dbSNP:rs9778Ensembl.1
Natural variantiVAR_022275257G → D2 PublicationsCorresponds to variant dbSNP:rs15303Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13450 mRNA Translation: AAA52408.1 Sequence problems.
AF112219 mRNA Translation: AAC99788.1
BT007059 mRNA Translation: AAP35708.1
AL136958 Genomic DNA Translation: CAI12225.1 Sequence problems.
AL136958 Genomic DNA Translation: CAI12226.1 Sequence problems.
AL136958 Genomic DNA Translation: CAI12228.1
BC001169 mRNA Translation: AAH01169.1
AF052509 Genomic DNA Translation: AAC06298.1
CCDSiCCDS9404.1
PIRiA23543
RefSeqiNP_001975.1, NM_001984.1
XP_005266335.1, XM_005266278.2
XP_011533256.1, XM_011534954.1
UniGeneiHs.432491

Genome annotation databases

EnsembliENST00000378720; ENSP00000367992; ENSG00000139684
GeneIDi2098
KEGGihsa:2098
UCSCiuc001vbn.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiESTD_HUMAN
AccessioniPrimary (citable) accession number: P10768
Secondary accession number(s): Q5TBU8
, Q5TBV0, Q5TBV2, Q9BVJ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 178 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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