Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P10764 (IGF2_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Insulin-like growth factor II

Short name=IGF-II

Cleaved into the following 2 chains:

  1. Insulin-like growth factor II
  2. Preptin
Gene names
Name:IGF2
OrganismOvis aries (Sheep) [Reference proteome]
Taxonomic identifier9940 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis

Protein attributes

Sequence length179 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.

Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3 By similarity.

Subcellular location

Secreted.

Sequence similarities

Belongs to the insulin family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Glucose metabolism
Osteogenesis
   Cellular componentSecreted
   DomainSignal
   Molecular functionGrowth factor
Hormone
Mitogen
   PTMCleavage on pair of basic residues
Disulfide bond
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processexocrine pancreas development

Inferred from electronic annotation. Source: Ensembl

glucose metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

insulin receptor signaling pathway via phosphatidylinositol 3-kinase

Inferred from electronic annotation. Source: Ensembl

organ morphogenesis

Inferred from electronic annotation. Source: Ensembl

osteoblast differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of MAPK cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of activated T cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell division

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of glycogen (starch) synthase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of glycogen biosynthetic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of insulin receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of mitosis

Inferred from electronic annotation. Source: Ensembl

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein kinase B signaling

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

striated muscle cell differentiation

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionprotein serine/threonine kinase activator activity

Inferred from electronic annotation. Source: Ensembl

receptor activator activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Ref.5 Ref.6
Chain25 – 9167Insulin-like growth factor II
PRO_0000015731
Propeptide92 – 17988E peptide
PRO_0000015732
Peptide93 – 12634Preptin
PRO_0000370381

Regions

Region25 – 5228B
Region53 – 6412C
Region65 – 8521A
Region86 – 916D

Amino acid modifications

Disulfide bond33 ↔ 71 By similarity
Disulfide bond45 ↔ 84 By similarity
Disulfide bond70 ↔ 75 By similarity

Experimental info

Sequence conflict46 – 472GD → DG AA sequence Ref.5

Sequences

Sequence LengthMass (Da)Tools
P10764 [UniParc].

Last modified October 1, 1989. Version 2.
Checksum: 7B369AE57F2E4378

FASTA17919,616
        10         20         30         40         50         60 
MGITAGKSML ALLAFLAFAS CCYAAYRPSE TLCGGELVDT LQFVCGDRGF YFSRPSSRIN 

        70         80         90        100        110        120 
RRSRGIVEEC CFRSCDLALL ETYCAAPAKS ERDVSASTTV LPDDFTAYPV GKFFQSDTWK 

       130        140        150        160        170 
QSTQRLRRGL PAFLRARRGR TLAKELEALR EAKSHRPLIA LPTQDPATHG GASSEASSD 

« Hide

References

[1]"Nucleotide sequence of an ovine insulin-like growth factor-II cDNA."
O'Mahoney J.V., Adams T.E.
Nucleic Acids Res. 17:5392-5392(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"The nucleotide and deduced amino acid sequences of insulin-like growth factor II cDNAs from adult bovine and fetal sheep liver."
Brown W.M., Dziegielewska K.M., Foreman R.C., Saunders N.R.
Nucleic Acids Res. 18:4614-4614(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[3]"Characterization of two sheep insulin-like growth factor II cDNAs with different 5'-untranslated regions."
Demmer J., Hill D.F., Petersen G.B.
Biochim. Biophys. Acta 1173:79-80(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: Coopworth.
Tissue: Liver.
[4]Ohlsen S.M., Wong E.A.
Submitted (SEP-1990) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Liver.
[5]"Sheep insulin-like growth factors I and II: sequences, activities and assays."
Francis G.L., McNeil K.A., Wallace J.C., Ballard F.J., Owens P.C.
Endocrinology 124:1173-1183(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-91.
[6]"Simultaneous isolation of insulin-like growth factors I and II from adult sheep serum."
Hey A.W., Browne C.A., Simpson R.J., Thorburn G.D.
Biochim. Biophys. Acta 997:27-35(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-58.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00668, U00666, U00667 Genomic DNA. Translation: AAB60626.1.
X15248 mRNA. Translation: CAA33324.1.
X53554 mRNA. Translation: CAA37621.1.
M89788 mRNA. Translation: AAA31548.1.
M89789 mRNA. Translation: AAA31549.1.
X55638 mRNA. Translation: CAA39163.1.
PIRS04858.
RefSeqNP_001009311.1. NM_001009311.1.
UniGeneOar.376.

3D structure databases

ProteinModelPortalP10764.
SMRP10764. Positions 25-91.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP10764.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID443325.

Organism-specific databases

CTD3481.

Phylogenomic databases

HOVERGENHBG006137.

Family and domain databases

Gene3D1.10.100.10. 1 hit.
InterProIPR022334. IGF2.
IPR013576. IGF2_C.
IPR016179. Insulin-like.
IPR022350. Insulin-like_growth_factor.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamPF08365. IGF2_C. 1 hit.
PF00049. Insulin. 1 hit.
[Graphical view]
PRINTSPR02002. INSLNLIKEGF.
PR02006. INSLNLIKEGF2.
PR00276. INSULINFAMLY.
ProDomPD005188. IGF2_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMSSF56994. SSF56994. 1 hit.
PROSITEPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIGF2_SHEEP
AccessionPrimary (citable) accession number: P10764
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 1, 1989
Last modified: July 9, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families