Reviewed, UniProtKB/Swiss-Prot P10759 (AMPD1_RAT)

Names and origin

Protein names

Recommended name:
    AMP deaminase 1
    EC=3.5.4.6
Alternative name(s):
    Myoadenylate deaminase
    AMP deaminase isoform M

Gene names

Name:

Ampd1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	747 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	AMP deaminase plays a critical role in energy metabolism.
Catalytic activity	AMP + H2O = IMP + NH3.
Pathway	Purine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1.
Subunit structure	Homotetramer.
Tissue specificity	Three isoforms are present in mammals: AMP deaminase 1 is the predominant form in skeletal muscle; AMP deaminase 2 predominates in smooth muscle, non-muscle tissue, embryonic muscle and undifferentiated myoblasts; AMP deaminase 3 is found in erythrocytes.
Sequence similarities	Belongs to the adenosine and AMP deaminases family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	purine base metabolic process Inferred from electronic annotation. Source: InterPro purine ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro response to organic substance Inferred from direct assay. Source: RGD
Molecular function	AMP deaminase activity Inferred from electronic annotation. Source: EC protein binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 747	747	AMP deaminase 1		PRO_0000194405
Sites
	Active site	363	1	Potential
	Active site	573	1	Potential
	Active site	649	1	Potential
	Active site	650	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	P10759-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: C8928B67F2DD9478	FASTA	747	86,432
	10 20 30 40 50 60 MPLFKLTGQG KQIDDAMRSF AEKVFASEVK DEGGRHEISP FDVDEICPIS LREMQAHIFH 70 80 90 100 110 120 MENLSMSMDG RRKRRFQGRK TVNLSIPQSE TSSTKLSHIE EFISSSPTYE SVPDFQRVQI 130 140 150 160 170 180 TGDYASGVTV EDFEVVCKGL YRALCIREKY MQKSFQRFPK TPSKYLRNID GEALVAIESF 190 200 210 220 230 240 YPVFTPPPKK GEDPFRREDL PANLGYHLKM KGGVIYIYPD EAAASRDEPK PYPYPNLDDF 250 260 270 280 290 300 LDDMNFLLAL IAQGPVKTYT HRRLKFLSSK FQVHQMLNEM DELKELKNNP HRDFYNCRKV 310 320 330 340 350 360 DTHIHAAACM NQKHLLRFIK KSYHIDADRV VYSTKEKNLT LKELFAQLNM HPYDLTVDSL 370 380 390 400 410 420 DVHAGRQTFQ RFDKFNDKYN PVGASELRDL YLKTDNYING EYFATIIKEV GADLVDAKYQ 430 440 450 460 470 480 HAEPRLSIYG RSPDEWSKLS SWFVGNRIYC PNMTWMIQVP RIYDVFRSKN FLPHFGKMLE 490 500 510 520 530 540 NIFLPVFEAT INPQTHPDLS VFLKHITGFD SVDDESKHSG HMFSSKSPKP EEWTMENNPS 550 560 570 580 590 600 YTYYAYYMYA NIMVLNCLRK ERGMNTFLFR PHCGEAGALT HLMTAFMIAD NISHGLNLKK 610 620 630 640 650 660 SPVLQYLFFL AQIPIAMSPL SNNSLFLEYA KNPFLDFLQK GLMISLSTDD PMQFHFTKEP 670 680 690 700 710 720 LMEEYAIAAQ VFKLSTCDMC EVARNSVLQC GISHEEKAKF LGNNYLEEGP VGNDIRRTNV 730 740 AQIRMAYRYE TWCYELNLIA EGLKSTE

References

[1]	"Cloning and sequence of rat myoadenylate deaminase cDNA. Evidence for tissue-specific and developmental regulation." Sabina R.L., Marquetant R., Desai N.M., Kaletha K., Holmes E.W. J. Biol. Chem. 262:12397-12400(1987) [PubMed: 3624265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 536-548. Tissue: Muscle.
[2]	"A novel pathway for alternative splicing: identification of an RNA intermediate that generates an alternative 5' splice donor site not present in the primary transcript of AMPD1." Mineo I., Clarke P.R., Sabina R.L., Holmes E.W. Mol. Cell. Biol. 10:5271-5278(1990) [PubMed: 2398891] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-17.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J02811 mRNA. Translation: AAB54086.1. M58688 mRNA. Translation: AAA40726.1.
IPI	IPI00193319.
PIR	A27366.
RefSeq	NP_620231.1.
UniGene	Rn.9794
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P10759.
Proteomic databases
PRIDE	P10759.
Genome annotation databases
Ensembl	ENSRNOT00000025248; ENSRNOP00000025248; ENSRNOG00000018656; Rattus norvegicus. [Genome view]
GeneID	25028.
KEGG	rno:25028.
UCSC	NM_138876. rat.
Organism-specific databases
CTD	25028.
RGD	2109. Ampd1.
Phylogenomic databases
HOVERGEN	P10759.
OMA	FAKLKMH.
Enzyme and pathway databases
BRENDA	3.5.4.6. 248.
Gene expression databases
ArrayExpress	P10759.
Genevestigator	P10759.
GermOnline	ENSRNOG00000018656. Rattus norvegicus.
Family and domain databases
InterPro	IPR006650. A/AMP_deam_AS. IPR001365. A/AMP_deaminase. IPR006329. AMP_deaminase. IPR016297. AMP_deaminase_met. [Graphical view]
Pfam	PF00962. A_deaminase. 1 hit. [Graphical view]
PIRSF	PIRSF001251. AMP_deaminase_met. 1 hit.
TIGRFAMs	TIGR01429. AMP_deaminase. 1 hit.
PROSITE	PS00485. A_DEAMINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	605167.

Entry information

Entry name

AMPD1_RAT

Accession

Primary (citable) accession number: P10759
Secondary accession number(s): P78501, Q6LDJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	November 3, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)