P10736 (VA52_DOLMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Venom allergen 5.01 Alternative name(s): Allergen Dol m V-A Antigen 5 form 2 Short name=Ag5-2 Cysteine-rich venom protein Short name=CRVP Allergen=Dol m 5.01
Organism	Dolichovespula maculata (Bald-faced hornet) (Vespula maculata)
Taxonomic identifier	7441 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Vespidae › Vespinae › Dolichovespula

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the CRISP family. Contains 1 SCP domain.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Domain	Signal
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23
	Chain	24 – 227	204	Venom allergen 5.01		PRO_0000006294
Regions
	Domain	68 – 212	145	SCP
Amino acid modifications
	Disulfide bond	27 ↔ 39		Ref.2
	Disulfide bond	31 ↔ 124		Ref.2
	Disulfide bond	49 ↔ 117		Ref.2
	Disulfide bond	193 ↔ 210		Ref.2
Natural variations
	Natural variant	54	1	V → A.

Sequences

	Sequence		Length	Mass (Da)
	P10736 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 4F0ED10D58C44502	FASTA	227	26,000
	10 20 30 40 50 60 MEIGGLVYLI LIITIINLSF GETNNYCKIK CRKGIHTLCK FGTSMKPNCG RNVVKAYGLT 70 80 90 100 110 120 NDEKNEILKR HNDFRQNVAK GLETRGKPGP QPPAKNMNVL VWNDELAKIA QTWANQCDFN 130 140 150 160 170 180 HDDCRNTAKY QVGQNIAISS TTATQFDRPS KLIKQWEDEV TEFNYKVGLQ NSNFRKVGHY 190 200 210 220 TQMVWGKTKE IGCGSIKYIE DNWYTHYLVC NYGPGGNDFN QPIYERK

References

[1]	"cDNA cloning and primary structure of a white-face hornet venom allergen, antigen 5." Fang K.S.Y., Vitale M., Fehlner P., King T.P. Proc. Natl. Acad. Sci. U.S.A. 85:895-899(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Venom and Venom gland.
[2]	"Structural studies of a hornet venom allergen antigen 5, Dol m V and its sequence similarity with other proteins." King T.P., Moran D., Wang D.F., Kochoumian L., Chait B.T. Protein Seq. Data Anal. 3:263-266(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, DISULFIDE BONDS. Tissue: Venom.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J03601 mRNA. Translation: AAA28301.1.
PIR	A31085.
3D structure databases
ProteinModelPortal	P10736.
SMR	P10736. Positions 21-227.
ModBase	Search...
Protein family/group databases
Allergome	1656. Dol m 5.0101. 330. Dol m 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.33.10. 1 hit.
InterPro	IPR001283. Allrgn_V5/Tpx1. IPR018244. Allrgn_V5/Tpx1_CS. IPR014044. CAP_domain. IPR002413. V5_allergen. [Graphical view]
PANTHER	PTHR10334. PTHR10334. 1 hit.
Pfam	PF00188. CAP. 1 hit. [Graphical view]
PRINTS	PR00838. V5ALLERGEN. PR00837. V5TPXLIKE.
SMART	SM00198. SCP. 1 hit. [Graphical view]
SUPFAM	SSF55797. SCP-like_extracellular. 1 hit.
PROSITE	PS01009. CRISP_1. 1 hit. PS01010. CRISP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VA52_DOLMA

Accession

Primary (citable) accession number: P10736

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)