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P10728

- SP2AB_BACSU

UniProt

P10728 - SP2AB_BACSU

Protein

Anti-sigma F factor

Gene

spoIIAB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Binds to sigma F and blocks its ability to form an RNA polymerase holoenzyme (E-sigma F). Phosphorylates SpoIIAA on a serine residue. This phosphorylation may enable SpoIIAA to act as an anti-anti-sigma factor that counteracts SpoIIAB and thus releases sigma F from inhibition.2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein serine/threonine kinase activity Source: UniProtKB-KW
    3. sigma factor antagonist activity Source: InterPro

    GO - Biological processi

    1. negative regulation of transcription, DNA-templated Source: InterPro
    2. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Sporulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU23460-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anti-sigma F factor (EC:2.7.11.1)
    Alternative name(s):
    Stage II sporulation protein AB
    Gene namesi
    Name:spoIIAB
    Ordered Locus Names:BSU23460
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU23460. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 146146Anti-sigma F factorPRO_0000203561Add
    BLAST

    Proteomic databases

    PaxDbiP10728.

    Expressioni

    Developmental stagei

    Interaction with SpoIIAB inhibits sigma F activity throughout the cell before the formation of the asymmetric septum; after septation the interaction is confined to the mother cell, and sigma F activity is released in the prespore.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU23460.

    Structurei

    3D structure databases

    ProteinModelPortaliP10728.
    SMRiP10728. Positions 2-137.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the anti-sigma-factor family.Curated

    Phylogenomic databases

    eggNOGiCOG2172.
    HOGENOMiHOG000269931.
    KOiK06379.
    OMAiDSSPEMG.
    OrthoDBiEOG6S7XW5.
    PhylomeDBiP10728.

    Family and domain databases

    Gene3Di3.30.565.10. 1 hit.
    HAMAPiMF_00637. Anti_sigma_F.
    InterProiIPR010194. Anti-sigma_F.
    IPR003594. HATPase_ATP-bd.
    [Graphical view]
    PfamiPF02518. HATPase_c. 1 hit.
    [Graphical view]
    SMARTiSM00387. HATPase_c. 1 hit.
    [Graphical view]
    SUPFAMiSSF55874. SSF55874. 1 hit.
    TIGRFAMsiTIGR01925. spIIAB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P10728-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKNEMHLEFS ALSQNESFAR VTVASFIAQL DPTMDELTEI KTVVSEAVTN    50
    AIIHGYEENC EGKVYISVTL EDHVVYMTIR DEGLGITDLE EARQPLFTTK 100
    PELERSGMGF TIMENFMDDV SIDSSPEMGT TIRLTKHLSK SKALCN 146
    Length:146
    Mass (Da):16,356
    Last modified:February 1, 1991 - v1
    Checksum:i141A28D41EDEC997
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17643 Genomic DNA. Translation: AAA22790.1.
    D84432 Genomic DNA. Translation: BAA12654.1.
    AL009126 Genomic DNA. Translation: CAB14278.1.
    PIRiB55646.
    RefSeqiNP_390227.1. NC_000964.3.
    WP_003230452.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB14278; CAB14278; BSU23460.
    GeneIDi938930.
    KEGGibsu:BSU23460.
    PATRICi18976511. VBIBacSub10457_2446.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17643 Genomic DNA. Translation: AAA22790.1 .
    D84432 Genomic DNA. Translation: BAA12654.1 .
    AL009126 Genomic DNA. Translation: CAB14278.1 .
    PIRi B55646.
    RefSeqi NP_390227.1. NC_000964.3.
    WP_003230452.1. NZ_CM000487.1.

    3D structure databases

    ProteinModelPortali P10728.
    SMRi P10728. Positions 2-137.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU23460.

    Proteomic databases

    PaxDbi P10728.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB14278 ; CAB14278 ; BSU23460 .
    GeneIDi 938930.
    KEGGi bsu:BSU23460.
    PATRICi 18976511. VBIBacSub10457_2446.

    Organism-specific databases

    GenoListi BSU23460. [Micado ]

    Phylogenomic databases

    eggNOGi COG2172.
    HOGENOMi HOG000269931.
    KOi K06379.
    OMAi DSSPEMG.
    OrthoDBi EOG6S7XW5.
    PhylomeDBi P10728.

    Enzyme and pathway databases

    BioCyci BSUB:BSU23460-MONOMER.

    Family and domain databases

    Gene3Di 3.30.565.10. 1 hit.
    HAMAPi MF_00637. Anti_sigma_F.
    InterProi IPR010194. Anti-sigma_F.
    IPR003594. HATPase_ATP-bd.
    [Graphical view ]
    Pfami PF02518. HATPase_c. 1 hit.
    [Graphical view ]
    SMARTi SM00387. HATPase_c. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55874. SSF55874. 1 hit.
    TIGRFAMsi TIGR01925. spIIAB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of sporulation locus spoIIA in Bacillus subtilis."
      Fort P., Piggot P.J.
      J. Gen. Microbiol. 130:2147-2153(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
      Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
      Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / JH642.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    4. "SpoIIAB is an anti-sigma factor that binds to and inhibits transcription by regulatory protein sigma F from Bacillus subtilis."
      Duncan L., Losick R.
      Proc. Natl. Acad. Sci. U.S.A. 90:2325-2329(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Sigma F, the first compartment-specific transcription factor of B. subtilis, is regulated by an anti-sigma factor that is also a protein kinase."
      Min K.-T., Hilditch C.M., Diederich B., Errington J., Yudkin M.D.
      Cell 74:735-742(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PROTEIN SEQUENCE OF 1-14.
      Strain: CU267.

    Entry informationi

    Entry nameiSP2AB_BACSU
    AccessioniPrimary (citable) accession number: P10728
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3