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P10728 (SP2AB_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anti-sigma F factor
EC=2.7.11.1
Alternative name(s):
Stage II sporulation protein AB
Gene names
Name:spoIIAB
Ordered Locus Names:BSU23460
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length146 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to sigma F and blocks its ability to form an RNA polymerase holoenzyme (E-sigma F). Phosphorylates SpoIIAA on a serine residue. This phosphorylation may enable SpoIIAA to act as an anti-anti-sigma factor that counteracts SpoIIAB and thus releases sigma F from inhibition. Ref.4 Ref.5

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. HAMAP-Rule MF_00637

Developmental stage

Interaction with SpoIIAB inhibits sigma F activity throughout the cell before the formation of the asymmetric septum; after septation the interaction is confined to the mother cell, and sigma F activity is released in the prespore. HAMAP-Rule MF_00637

Sequence similarities

Belongs to the anti-sigma-factor family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 146146Anti-sigma F factor HAMAP-Rule MF_00637
PRO_0000203561

Sequences

Sequence LengthMass (Da)Tools
P10728 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 141A28D41EDEC997

FASTA14616,356
        10         20         30         40         50         60 
MKNEMHLEFS ALSQNESFAR VTVASFIAQL DPTMDELTEI KTVVSEAVTN AIIHGYEENC 

        70         80         90        100        110        120 
EGKVYISVTL EDHVVYMTIR DEGLGITDLE EARQPLFTTK PELERSGMGF TIMENFMDDV 

       130        140 
SIDSSPEMGT TIRLTKHLSK SKALCN

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of sporulation locus spoIIA in Bacillus subtilis."
Fort P., Piggot P.J.
J. Gen. Microbiol. 130:2147-2153(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / JH642.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"SpoIIAB is an anti-sigma factor that binds to and inhibits transcription by regulatory protein sigma F from Bacillus subtilis."
Duncan L., Losick R.
Proc. Natl. Acad. Sci. U.S.A. 90:2325-2329(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Sigma F, the first compartment-specific transcription factor of B. subtilis, is regulated by an anti-sigma factor that is also a protein kinase."
Min K.-T., Hilditch C.M., Diederich B., Errington J., Yudkin M.D.
Cell 74:735-742(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PROTEIN SEQUENCE OF 1-14.
Strain: CU267.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M17643 Genomic DNA. Translation: AAA22790.1.
D84432 Genomic DNA. Translation: BAA12654.1.
AL009126 Genomic DNA. Translation: CAB14278.1.
PIRB55646.
RefSeqNP_390227.1. NC_000964.3.

3D structure databases

ProteinModelPortalP10728.
SMRP10728. Positions 2-137.
ModBaseSearch...

Protein-protein interaction databases

STRING224308.BSU23460.

Proteomic databases

PaxDbP10728.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB14278; CAB14278; BSU23460.
GeneID938930.
KEGGbsu:BSU23460.
PATRIC18976511. VBIBacSub10457_2446.

Organism-specific databases

GenoListBSU23460. [Micado]

Phylogenomic databases

eggNOGCOG2172.
HOGENOMHOG000269931.
KOK06379.
OMADSSPEMG.
ProtClustDBPRK03660.

Enzyme and pathway databases

BioCycBSUB:BSU23460-MONOMER.

Family and domain databases

Gene3D3.30.565.10. 1 hit.
HAMAPMF_00637. Anti_sigma_F.
InterProIPR010194. Anti-sigma_F.
IPR003594. HATPase_ATP-bd.
[Graphical view]
PfamPF02518. HATPase_c. 1 hit.
[Graphical view]
SMARTSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMSSF55874. ATP_bd_ATPase. 1 hit.
TIGRFAMsTIGR01925. spIIAB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSP2AB_BACSU
AccessionPrimary (citable) accession number: P10728
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

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