P10727 (SP2AA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anti-sigma F factor antagonist

Alternative name(s):
Stage II sporulation protein AA

Gene names

Name:	spoIIAA
Ordered Locus Names:	BSU23470

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	117 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	In the phosphorylated form it could act as an anti-anti-sigma factor that counteracts SpoIIAB and thus releases sigma f from inhibition. Ref.5
Post-translational modification	Phosphorylated by SpoIIAB on a serine residue. Ref.6
Sequence similarities	Belongs to the anti-sigma-factor antagonist family. Contains 1 STAS domain.
Sequence caution	The sequence AAA22789.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Sporulation
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.4 Ref.5

Chain

2 – 117

116

Anti-sigma F factor antagonist

PRO_0000194204

Regions

Domain

3 – 113

111

STAS

Amino acid modifications

Modified residue

58

1

Phosphoserine Ref.6

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

117

Helix Strand Turn

Details...

Beta strand

7 – 10

4

Beta strand

13 – 16

4

Helix

26 – 38

13

Beta strand

45 – 49

5

Helix

60 – 74

15

Turn

85 – 88

4

Helix

89 – 94

6

Helix

97 – 99

3

Helix

106 – 108

3

Helix

110 – 112

3

Sequences

	Sequence		Length	Mass (Da)
	P10727 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 2333E74207568AC2	FASTA	117	12,990
	10 20 30 40 50 60 MSLGIDMNVK ESVLCIRLTG ELDHHTAETL KQKVTQSLEK DDIRHIVLNL EDLSFMDSSG 70 80 90 100 110 LGVILGRYKQ IKQIGGEMVV CAISPAVKRL FDMSGLFKII RFEQSEQQAL LTLGVAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of sporulation locus spoIIA in Bacillus subtilis." Fort P., Piggot P.J. J. Gen. Microbiol. 130:2147-2153(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CU267.
[2]	"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes." Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y. Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Characterization of a Bacillus subtilis sporulation operon that includes genes for an RNA polymerase sigma factor and for a putative DD-carboxypeptidase." Wu J.-J., Schuch R., Piggot P.J. J. Bacteriol. 174:4885-4892(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-4.
[5]	"Sigma F, the first compartment-specific transcription factor of B. subtilis, is regulated by an anti-sigma factor that is also a protein kinase." Min K.-T., Hilditch C.M., Diederich B., Errington J., Yudkin M.D. Cell 74:735-742(1993) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PROTEIN SEQUENCE OF 2-14.
[6]	"Site of phosphorylation of SpoIIAA, the anti-anti-sigma factor for sporulation-specific sigma F of Bacillus subtilis." Najafi S.M.A., Willis A.C., Yudkin M.D. J. Bacteriol. 177:2912-2913(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-58, PROTEIN SEQUENCE OF 52-67.
[7]	"Solution structure of SpoIIAA, a phosphorylatable component of the system that regulates transcription factor sigmaF of Bacillus subtilis." Kovacs H., Comfort D., Lord M., Campbell I.D., Yudkin M.D. Proc. Natl. Acad. Sci. U.S.A. 95:5067-5071(1998) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M17643 Genomic DNA. Translation: AAA22789.1. Different initiation.
D84432 Genomic DNA. Translation: BAA12653.1.
AL009126 Genomic DNA. Translation: CAB14279.1.

PIR

A55646.

RefSeq

NP_390228.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AUZ	NMR	-	A	2-117	[»]
1BUZ	NMR	-	A	2-117	[»]

ProteinModelPortal

P10727.

SMR

P10727. Positions 2-117.

ModBase

Search...

Protein-protein interaction databases

STRING

224308.BSU23470.

PTM databases

PhosSite

P010322.

Proteomic databases

PaxDb

P10727.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAB14279; CAB14279; BSU23470.

GeneID

938730.

KEGG

bsu:BSU23470.

PATRIC

18976513. VBIBacSub10457_2447.

Organism-specific databases

GenoList

BSU23470. [Micado]

Phylogenomic databases

eggNOG

COG1366.

HOGENOM

HOG000270156.

KO

K06378.

OMA

CSINEQV.

ProtClustDB

CLSK873052.

Enzyme and pathway databases

BioCyc

BSUB:BSU23470-MONOMER.

Family and domain databases

Gene3D

3.30.750.24. 1 hit.

InterPro

IPR003658. Anti-sigma_ant.
IPR014237. Anti-sigma_F_ant.
IPR002645. STAS_dom.
[Graphical view]

Pfam

PF01740. STAS. 1 hit.
[Graphical view]

SUPFAM

SSF52091. STAS. 1 hit.

TIGRFAMs

TIGR00377. ant_ant_sig. 1 hit.
TIGR02886. spore_II_AA. 1 hit.

PROSITE

PS50801. STAS. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P10727.

Entry information

Entry name

SP2AA_BACSU

Accession

Primary (citable) accession number: P10727

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 115 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program