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P10727 (SP2AA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anti-sigma F factor antagonist
Alternative name(s):
Stage II sporulation protein AA
Gene names
Name:spoIIAA
Ordered Locus Names:BSU23470
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length117 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

In the phosphorylated form it could act as an anti-anti-sigma factor that counteracts SpoIIAB and thus releases sigma f from inhibition. Ref.5

Post-translational modification

Phosphorylated by SpoIIAB on a serine residue. Ref.6

Sequence similarities

Belongs to the anti-sigma-factor antagonist family.

Contains 1 STAS domain.

Sequence caution

The sequence AAA22789.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4 Ref.5
Chain2 – 117116Anti-sigma F factor antagonist
PRO_0000194204

Regions

Domain3 – 113111STAS

Amino acid modifications

Modified residue581Phosphoserine Ref.6

Secondary structure

.................... 117
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P10727 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 2333E74207568AC2

FASTA11712,990
        10         20         30         40         50         60 
MSLGIDMNVK ESVLCIRLTG ELDHHTAETL KQKVTQSLEK DDIRHIVLNL EDLSFMDSSG 

        70         80         90        100        110 
LGVILGRYKQ IKQIGGEMVV CAISPAVKRL FDMSGLFKII RFEQSEQQAL LTLGVAS 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of sporulation locus spoIIA in Bacillus subtilis."
Fort P., Piggot P.J.
J. Gen. Microbiol. 130:2147-2153(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: CU267.
[2]"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / JH642.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"Characterization of a Bacillus subtilis sporulation operon that includes genes for an RNA polymerase sigma factor and for a putative DD-carboxypeptidase."
Wu J.-J., Schuch R., Piggot P.J.
J. Bacteriol. 174:4885-4892(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-4.
[5]"Sigma F, the first compartment-specific transcription factor of B. subtilis, is regulated by an anti-sigma factor that is also a protein kinase."
Min K.-T., Hilditch C.M., Diederich B., Errington J., Yudkin M.D.
Cell 74:735-742(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PROTEIN SEQUENCE OF 2-14.
[6]"Site of phosphorylation of SpoIIAA, the anti-anti-sigma factor for sporulation-specific sigma F of Bacillus subtilis."
Najafi S.M.A., Willis A.C., Yudkin M.D.
J. Bacteriol. 177:2912-2913(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-58, PROTEIN SEQUENCE OF 52-67.
[7]"Solution structure of SpoIIAA, a phosphorylatable component of the system that regulates transcription factor sigmaF of Bacillus subtilis."
Kovacs H., Comfort D., Lord M., Campbell I.D., Yudkin M.D.
Proc. Natl. Acad. Sci. U.S.A. 95:5067-5071(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M17643 Genomic DNA. Translation: AAA22789.1. Different initiation.
D84432 Genomic DNA. Translation: BAA12653.1.
AL009126 Genomic DNA. Translation: CAB14279.1.
PIRA55646.
RefSeqNP_390228.1. NC_000964.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AUZNMR-A2-117[»]
1BUZNMR-A2-117[»]
ProteinModelPortalP10727.
SMRP10727. Positions 2-117.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU23470.

PTM databases

PhosSiteP010322.

Proteomic databases

PaxDbP10727.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB14279; CAB14279; BSU23470.
GeneID938730.
KEGGbsu:BSU23470.
PATRIC18976513. VBIBacSub10457_2447.

Organism-specific databases

GenoListBSU23470. [Micado]

Phylogenomic databases

eggNOGCOG1366.
HOGENOMHOG000270156.
KOK06378.
OMAHLEVKRD.
OrthoDBEOG6J48NK.
PhylomeDBP10727.

Enzyme and pathway databases

BioCycBSUB:BSU23470-MONOMER.

Family and domain databases

Gene3D3.30.750.24. 1 hit.
InterProIPR003658. Anti-sigma_ant.
IPR014237. Anti-sigma_F_ant.
IPR002645. STAS_dom.
[Graphical view]
PfamPF01740. STAS. 1 hit.
[Graphical view]
SUPFAMSSF52091. SSF52091. 1 hit.
TIGRFAMsTIGR00377. ant_ant_sig. 1 hit.
TIGR02886. spore_II_AA. 1 hit.
PROSITEPS50801. STAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP10727.

Entry information

Entry nameSP2AA_BACSU
AccessionPrimary (citable) accession number: P10727
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList