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Protein

Anti-sigma F factor antagonist

Gene

spoIIAA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

In the phosphorylated form it could act as an anti-anti-sigma factor that counteracts SpoIIAB and thus releases sigma f from inhibition.1 Publication

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: InterPro
  2. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Sporulation

Enzyme and pathway databases

BioCyciBSUB:BSU23470-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Anti-sigma F factor antagonist
Alternative name(s):
Stage II sporulation protein AA
Gene namesi
Name:spoIIAA
Ordered Locus Names:BSU23470
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570 Componenti: Chromosome

Organism-specific databases

GenoListiBSU23470. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 117116Anti-sigma F factor antagonistPRO_0000194204Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei58 – 581Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated by SpoIIAB on a serine residue.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP10727.

Interactioni

Protein-protein interaction databases

IntActiP10727. 1 interaction.
STRINGi224308.BSU23470.

Structurei

Secondary structure

1
117
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 104Combined sources
Beta strandi13 – 164Combined sources
Helixi26 – 3813Combined sources
Beta strandi45 – 495Combined sources
Helixi60 – 7415Combined sources
Turni85 – 884Combined sources
Helixi89 – 946Combined sources
Helixi97 – 993Combined sources
Helixi106 – 1083Combined sources
Helixi110 – 1123Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AUZNMR-A2-117[»]
1BUZNMR-A2-117[»]
ProteinModelPortaliP10727.
SMRiP10727. Positions 2-117.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10727.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 113111STASPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 STAS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1366.
HOGENOMiHOG000270156.
InParanoidiP10727.
KOiK06378.
OMAiCSINEQV.
OrthoDBiEOG6J48NK.
PhylomeDBiP10727.

Family and domain databases

Gene3Di3.30.750.24. 1 hit.
InterProiIPR003658. Anti-sigma_ant.
IPR014237. Anti-sigma_F_ant.
IPR002645. STAS_dom.
[Graphical view]
PfamiPF01740. STAS. 1 hit.
[Graphical view]
SUPFAMiSSF52091. SSF52091. 1 hit.
TIGRFAMsiTIGR00377. ant_ant_sig. 1 hit.
TIGR02886. spore_II_AA. 1 hit.
PROSITEiPS50801. STAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10727-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLGIDMNVK ESVLCIRLTG ELDHHTAETL KQKVTQSLEK DDIRHIVLNL
60 70 80 90 100
EDLSFMDSSG LGVILGRYKQ IKQIGGEMVV CAISPAVKRL FDMSGLFKII
110
RFEQSEQQAL LTLGVAS
Length:117
Mass (Da):12,990
Last modified:January 22, 2007 - v3
Checksum:i2333E74207568AC2
GO

Sequence cautioni

The sequence AAA22789.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17643 Genomic DNA. Translation: AAA22789.1. Different initiation.
D84432 Genomic DNA. Translation: BAA12653.1.
AL009126 Genomic DNA. Translation: CAB14279.1.
PIRiA55646.
RefSeqiNP_390228.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB14279; CAB14279; BSU23470.
GeneIDi938730.
KEGGibsu:BSU23470.
PATRICi18976513. VBIBacSub10457_2447.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17643 Genomic DNA. Translation: AAA22789.1. Different initiation.
D84432 Genomic DNA. Translation: BAA12653.1.
AL009126 Genomic DNA. Translation: CAB14279.1.
PIRiA55646.
RefSeqiNP_390228.1. NC_000964.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AUZNMR-A2-117[»]
1BUZNMR-A2-117[»]
ProteinModelPortaliP10727.
SMRiP10727. Positions 2-117.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP10727. 1 interaction.
STRINGi224308.BSU23470.

Proteomic databases

PaxDbiP10727.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14279; CAB14279; BSU23470.
GeneIDi938730.
KEGGibsu:BSU23470.
PATRICi18976513. VBIBacSub10457_2447.

Organism-specific databases

GenoListiBSU23470. [Micado]

Phylogenomic databases

eggNOGiCOG1366.
HOGENOMiHOG000270156.
InParanoidiP10727.
KOiK06378.
OMAiCSINEQV.
OrthoDBiEOG6J48NK.
PhylomeDBiP10727.

Enzyme and pathway databases

BioCyciBSUB:BSU23470-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP10727.

Family and domain databases

Gene3Di3.30.750.24. 1 hit.
InterProiIPR003658. Anti-sigma_ant.
IPR014237. Anti-sigma_F_ant.
IPR002645. STAS_dom.
[Graphical view]
PfamiPF01740. STAS. 1 hit.
[Graphical view]
SUPFAMiSSF52091. SSF52091. 1 hit.
TIGRFAMsiTIGR00377. ant_ant_sig. 1 hit.
TIGR02886. spore_II_AA. 1 hit.
PROSITEiPS50801. STAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of sporulation locus spoIIA in Bacillus subtilis."
    Fort P., Piggot P.J.
    J. Gen. Microbiol. 130:2147-2153(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CU267.
  2. "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
    Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
    Microbiology 142:3103-3111(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / JH642.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "Characterization of a Bacillus subtilis sporulation operon that includes genes for an RNA polymerase sigma factor and for a putative DD-carboxypeptidase."
    Wu J.-J., Schuch R., Piggot P.J.
    J. Bacteriol. 174:4885-4892(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-4.
  5. "Sigma F, the first compartment-specific transcription factor of B. subtilis, is regulated by an anti-sigma factor that is also a protein kinase."
    Min K.-T., Hilditch C.M., Diederich B., Errington J., Yudkin M.D.
    Cell 74:735-742(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PROTEIN SEQUENCE OF 2-14.
  6. "Site of phosphorylation of SpoIIAA, the anti-anti-sigma factor for sporulation-specific sigma F of Bacillus subtilis."
    Najafi S.M.A., Willis A.C., Yudkin M.D.
    J. Bacteriol. 177:2912-2913(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-58, PROTEIN SEQUENCE OF 52-67.
  7. "Solution structure of SpoIIAA, a phosphorylatable component of the system that regulates transcription factor sigmaF of Bacillus subtilis."
    Kovacs H., Comfort D., Lord M., Campbell I.D., Yudkin M.D.
    Proc. Natl. Acad. Sci. U.S.A. 95:5067-5071(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiSP2AA_BACSU
AccessioniPrimary (citable) accession number: P10727
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1991
Last sequence update: January 22, 2007
Last modified: March 3, 2015
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.