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Protein

Alanine racemase 1

Gene

alr1

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.UniRule annotation

Catalytic activityi

L-alanine = D-alanine.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei41 – 411Proton acceptor; specific for D-alanineUniRule annotation
Binding sitei137 – 1371SubstrateUniRule annotation
Active sitei266 – 2661Proton acceptor; specific for L-alanineUniRule annotation
Binding sitei313 – 3131Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. alanine racemase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. D-alanine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciBSUB:BSU04640-MONOMER.
BRENDAi5.1.1.1. 658.
SABIO-RKP10725.
UniPathwayiUPA00042; UER00497.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemase 1UniRule annotation (EC:5.1.1.1UniRule annotation)
Gene namesi
Name:alr1
Synonyms:alr, dal
Ordered Locus Names:BSU04640
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570 Componenti: Chromosome

Organism-specific databases

GenoListiBSU04640. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 389389Alanine racemase 1PRO_0000114500Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei41 – 411N6-(pyridoxal phosphate)lysineUniRule annotation

Proteomic databases

PaxDbiP10725.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU04640.

Structurei

3D structure databases

ProteinModelPortaliP10725.
SMRiP10725. Positions 4-382.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0787.
HOGENOMiHOG000031444.
InParanoidiP10725.
KOiK01775.
OMAiITMDQLM.
OrthoDBiEOG6PP9NJ.
PhylomeDBiP10725.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10725-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTKPFYRDT WAEIDLSAIK ENVSNMKKHI GEHVHLMAVV KANAYGHGDA
60 70 80 90 100
ETAKAALDAG ASCLAVAILD EAISLRKKGL KAPILVLGAV PPEYVAIAAE
110 120 130 140 150
YDVTLTGYSV EWLQEAARHT KKGSLHFHLK VDTGMNRLGV KTEEEVQNVM
160 170 180 190 200
AILDRNPRLK CKGVFTHFAT ADEKERGYFL MQFERFKELI APLPLKNLMV
210 220 230 240 250
HCANSAAGLR LKKGFFNAVR FGIGMYGLRP SADMSDEIPF QLRPAFTLHS
260 270 280 290 300
TLSHVKLIRK GESVSYGAEY TAEKDTWIGT VPVGYADGWL RKLKGTDILV
310 320 330 340 350
KGKRLKIAGR ICMDQFMVEL DQEYPPGTKV TLIGRQGDEY ISMDEIAGRL
360 370 380
ETINYEVACT ISSRVPRMFL ENGSIMEVRN PLLQVNISN
Length:389
Mass (Da):43,265
Last modified:December 14, 1998 - v2
Checksum:i4802B7C182ACCD58
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401V → E in AAA22378 (Ref. 1) Curated
Sequence conflicti66 – 661V → M in AAA22378 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16207 Genomic DNA. Translation: AAA22378.1.
AB001488 Genomic DNA. Translation: BAA19301.1.
AL009126 Genomic DNA. Translation: CAB12271.1.
PIRiJS0443.
RefSeqiNP_388345.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB12271; CAB12271; BSU04640.
GeneIDi939942.
KEGGibsu:BSU04640.
PATRICi18972512. VBIBacSub10457_0484.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16207 Genomic DNA. Translation: AAA22378.1.
AB001488 Genomic DNA. Translation: BAA19301.1.
AL009126 Genomic DNA. Translation: CAB12271.1.
PIRiJS0443.
RefSeqiNP_388345.1. NC_000964.3.

3D structure databases

ProteinModelPortaliP10725.
SMRiP10725. Positions 4-382.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU04640.

Proteomic databases

PaxDbiP10725.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12271; CAB12271; BSU04640.
GeneIDi939942.
KEGGibsu:BSU04640.
PATRICi18972512. VBIBacSub10457_0484.

Organism-specific databases

GenoListiBSU04640. [Micado]

Phylogenomic databases

eggNOGiCOG0787.
HOGENOMiHOG000031444.
InParanoidiP10725.
KOiK01775.
OMAiITMDQLM.
OrthoDBiEOG6PP9NJ.
PhylomeDBiP10725.

Enzyme and pathway databases

UniPathwayiUPA00042; UER00497.
BioCyciBSUB:BSU04640-MONOMER.
BRENDAi5.1.1.1. 658.
SABIO-RKP10725.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of an alanine racemase gene from Bacillus subtilis and its use for plasmid maintenance in B. subtilis."
    Ferrari E., Henner D.J., Yang M.Y.
    Biotechnology (N.Y.) 3:1003-1007(1984)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus subtilis genome."
    Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.
    Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiALR1_BACSU
AccessioniPrimary (citable) accession number: P10725
Secondary accession number(s): P96620
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 30, 1989
Last sequence update: December 14, 1998
Last modified: March 31, 2015
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.