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P10725

- ALR1_BACSU

UniProt

P10725 - ALR1_BACSU

Protein

Alanine racemase 1

Gene

alr1

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (15 Dec 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.UniRule annotation

    Catalytic activityi

    L-alanine = D-alanine.UniRule annotation

    Cofactori

    Pyridoxal phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei41 – 411Proton acceptor; specific for D-alanineUniRule annotation
    Binding sitei137 – 1371SubstrateUniRule annotation
    Active sitei266 – 2661Proton acceptor; specific for L-alanineUniRule annotation
    Binding sitei313 – 3131Substrate; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. alanine racemase activity Source: UniProtKB-HAMAP
    2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. D-alanine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciBSUB:BSU04640-MONOMER.
    SABIO-RKP10725.
    UniPathwayiUPA00042; UER00497.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine racemase 1UniRule annotation (EC:5.1.1.1UniRule annotation)
    Gene namesi
    Name:alr1
    Synonyms:alr, dal
    Ordered Locus Names:BSU04640
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU04640. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 389389Alanine racemase 1PRO_0000114500Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei41 – 411N6-(pyridoxal phosphate)lysineUniRule annotation

    Proteomic databases

    PaxDbiP10725.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU04640.

    Structurei

    3D structure databases

    ProteinModelPortaliP10725.
    SMRiP10725. Positions 4-382.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alanine racemase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0787.
    HOGENOMiHOG000031444.
    KOiK01775.
    OMAiIYSHLAL.
    OrthoDBiEOG6PP9NJ.
    PhylomeDBiP10725.

    Family and domain databases

    Gene3Di2.40.37.10. 1 hit.
    3.20.20.10. 1 hit.
    HAMAPiMF_01201. Ala_racemase.
    InterProiIPR000821. Ala_racemase.
    IPR009006. Ala_racemase/Decarboxylase_C.
    IPR011079. Ala_racemase_C.
    IPR001608. Ala_racemase_N.
    IPR020622. Ala_racemase_pyridoxalP-BS.
    IPR029066. PLP-binding_barrel.
    [Graphical view]
    PfamiPF00842. Ala_racemase_C. 1 hit.
    PF01168. Ala_racemase_N. 1 hit.
    [Graphical view]
    PRINTSiPR00992. ALARACEMASE.
    SMARTiSM01005. Ala_racemase_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF50621. SSF50621. 1 hit.
    SSF51419. SSF51419. 1 hit.
    TIGRFAMsiTIGR00492. alr. 1 hit.
    PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P10725-1 [UniParc]FASTAAdd to Basket

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    MSTKPFYRDT WAEIDLSAIK ENVSNMKKHI GEHVHLMAVV KANAYGHGDA    50
    ETAKAALDAG ASCLAVAILD EAISLRKKGL KAPILVLGAV PPEYVAIAAE 100
    YDVTLTGYSV EWLQEAARHT KKGSLHFHLK VDTGMNRLGV KTEEEVQNVM 150
    AILDRNPRLK CKGVFTHFAT ADEKERGYFL MQFERFKELI APLPLKNLMV 200
    HCANSAAGLR LKKGFFNAVR FGIGMYGLRP SADMSDEIPF QLRPAFTLHS 250
    TLSHVKLIRK GESVSYGAEY TAEKDTWIGT VPVGYADGWL RKLKGTDILV 300
    KGKRLKIAGR ICMDQFMVEL DQEYPPGTKV TLIGRQGDEY ISMDEIAGRL 350
    ETINYEVACT ISSRVPRMFL ENGSIMEVRN PLLQVNISN 389
    Length:389
    Mass (Da):43,265
    Last modified:December 15, 1998 - v2
    Checksum:i4802B7C182ACCD58
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti40 – 401V → E in AAA22378. 1 PublicationCurated
    Sequence conflicti66 – 661V → M in AAA22378. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16207 Genomic DNA. Translation: AAA22378.1.
    AB001488 Genomic DNA. Translation: BAA19301.1.
    AL009126 Genomic DNA. Translation: CAB12271.1.
    PIRiJS0443.
    RefSeqiNP_388345.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB12271; CAB12271; BSU04640.
    GeneIDi939942.
    KEGGibsu:BSU04640.
    PATRICi18972512. VBIBacSub10457_0484.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16207 Genomic DNA. Translation: AAA22378.1 .
    AB001488 Genomic DNA. Translation: BAA19301.1 .
    AL009126 Genomic DNA. Translation: CAB12271.1 .
    PIRi JS0443.
    RefSeqi NP_388345.1. NC_000964.3.

    3D structure databases

    ProteinModelPortali P10725.
    SMRi P10725. Positions 4-382.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU04640.

    Proteomic databases

    PaxDbi P10725.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB12271 ; CAB12271 ; BSU04640 .
    GeneIDi 939942.
    KEGGi bsu:BSU04640.
    PATRICi 18972512. VBIBacSub10457_0484.

    Organism-specific databases

    GenoListi BSU04640. [Micado ]

    Phylogenomic databases

    eggNOGi COG0787.
    HOGENOMi HOG000031444.
    KOi K01775.
    OMAi IYSHLAL.
    OrthoDBi EOG6PP9NJ.
    PhylomeDBi P10725.

    Enzyme and pathway databases

    UniPathwayi UPA00042 ; UER00497 .
    BioCyci BSUB:BSU04640-MONOMER.
    SABIO-RK P10725.

    Family and domain databases

    Gene3Di 2.40.37.10. 1 hit.
    3.20.20.10. 1 hit.
    HAMAPi MF_01201. Ala_racemase.
    InterProi IPR000821. Ala_racemase.
    IPR009006. Ala_racemase/Decarboxylase_C.
    IPR011079. Ala_racemase_C.
    IPR001608. Ala_racemase_N.
    IPR020622. Ala_racemase_pyridoxalP-BS.
    IPR029066. PLP-binding_barrel.
    [Graphical view ]
    Pfami PF00842. Ala_racemase_C. 1 hit.
    PF01168. Ala_racemase_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00992. ALARACEMASE.
    SMARTi SM01005. Ala_racemase_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50621. SSF50621. 1 hit.
    SSF51419. SSF51419. 1 hit.
    TIGRFAMsi TIGR00492. alr. 1 hit.
    PROSITEi PS00395. ALANINE_RACEMASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of an alanine racemase gene from Bacillus subtilis and its use for plasmid maintenance in B. subtilis."
      Ferrari E., Henner D.J., Yang M.Y.
      Biotechnology (N.Y.) 3:1003-1007(1985)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus subtilis genome."
      Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.
      Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.

    Entry informationi

    Entry nameiALR1_BACSU
    AccessioniPrimary (citable) accession number: P10725
    Secondary accession number(s): P96620
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3