P10723 (SYNC_BRUMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Asparagine--tRNA ligase, cytoplasmic EC=6.1.1.22 Alternative name(s): Asparaginyl-tRNA synthetase Short name=AsnRS Potentially protective 63 kDa antigen
Organism	Brugia malayi (Filarial nematode worm)
Taxonomic identifier	6279 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Nematoda › Chromadorea › Spirurida › Filarioidea › Onchocercidae › Brugia

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Potentially protective antigen in lymphatic filariasis. Ref.3
Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 548

548

Asparagine--tRNA ligase, cytoplasmic

PRO_0000176494

Regions

Region

81 – 131

51

Region of immunological reactivity

Experimental info

Sequence conflict

50

1

I → V in AAA27849. Ref.2

Sequence conflict

65

1

H → R in AAA27849. Ref.2

Sequence conflict

212

1

P → S in AAA27849. Ref.2

Sequence conflict

265

1

L → V in AAA27849. Ref.2

Sequence conflict

454

1

Q → K in AAA27849. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

548

Helix Strand Turn

Details...

Beta strand

3 – 5

3

Helix

25 – 32

8

Beta strand

39 – 44

6

Beta strand

49 – 53

5

Helix

56 – 72

17

Sequences

	Sequence		Length	Mass (Da)
	P10723 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 7E4F6EA248255095	FASTA	548	62,339
	10 20 30 40 50 60 MTVYICPETG DDGNDGSELK PLRTLYQAMI ITKSSKGDFL IRTKKDGKQI WEAASKTALK 70 80 90 100 110 120 KSWKHYEQEM LKNEKVAAKM LEKDATEVGV KAALEEAKKV QIELDTSLSY ITGVKIRDLV 130 140 150 160 170 180 KHRNERVCIK GWIHRMRRQG KSLMFFILRD GTGFLQVLLM DKLCQTYDAL TVNTECTVEI 190 200 210 220 230 240 YGAIKEVPEG KEAPNGHELI ADFWKIIGNA PPGGIDNVLN EEASVDKMLD NRHLVIRGEN 250 260 270 280 290 300 AAALLRLRAA ATRAMREHFY NAGYLEVAPP TLVQTQVEGG STLFNLDYFG EQSFLTQSSQ 310 320 330 340 350 360 LYLETCIPTL GDVFLHCSVL QGGKISHSST LAEYAHVEAE CPFITLDDLM EKIEELVCDT 370 380 390 400 410 420 VDRLLADEEA KKLLEHINPK FQPPERPFLR MEYKDAIKWL QEHNVENEFG NTFTYGEDIA 430 440 450 460 470 480 EAAERFMTDT INKPILLNRF PSEIKAFYMQ RDAQDNTLTE SVDLLMPGVG EIVGGSMRIW 490 500 510 520 530 540 KFDELSKAFK NVEIDPKPYY WYLDQRLYGT CPHGGYGLGL ERFICWLTNT NHIRDVCLYP RFVGRCVP

References

[1]	"A multi-copy gene encodes a potentially protective antigen in Brugia malayi." Perrine K.G., Denker J.A., Nilsen T.W. Mol. Biochem. Parasitol. 30:97-104(1988) [PubMed: 2840577] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and characterization of a potentially protective antigen in lymphatic filariasis." Nilsen T.W., Maroney P.A., Goodwin R.G., Perrine K.G., Denker J.A., Nanduri J., Kazura J.W. Proc. Natl. Acad. Sci. U.S.A. 85:3604-3607(1988) [PubMed: 3368467] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"An immunodominant antigen of Brugia malayi is an asparaginyl-tRNA synthetase." Kron M., Marquard K., Hartlein M., Price S., Leberman R. FEBS Lett. 374:122-124(1995) [PubMed: 7589498] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J03971 Genomic DNA. Translation: AAA27852.1.
J03266 mRNA. Translation: AAA27849.1.

PIR

A28209.
A54510.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KQR	NMR	-	A	1-111	[»]

ProteinModelPortal

P10723.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-synth_IIb.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR004365. NA-bd_OB_tRNA-helicase.
[Graphical view]

Gene3D

G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.

PANTHER

PTHR22594. aa-tRNA-synt_II. 1 hit.
PTHR22594:SF6. PTHR22594:SF6. 1 hit.

Pfam

PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti. 1 hit.
[Graphical view]

PRINTS

PR01042. TRNASYNTHASP.

SUPFAM

SSF50249. Nucleic_acid_OB. 1 hit.

TIGRFAMs

TIGR00457. AsnS. 1 hit.

PROSITE

PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

SYNC_BRUMA

Accession

Primary (citable) accession number: P10723

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)