P10720 (PF4V_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Platelet factor 4 variant

Alternative name(s):
C-X-C motif chemokine 4 variant
CXCL4L1
PF4alt
PF4var1

Cleaved into the following 3 chains:

1. Platelet factor 4 variant(4-74)
2. Platelet factor 4 variant(5-74)
3. Platelet factor 4 variant(6-74)

Gene names

Name:	PF4V1
Synonyms:	CXCL4V1, SCYB4V1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	104 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibitor of angiogenesis. Inhibitor of endothelial cell chemotaxis (in vitro). Ref.5
Subcellular location	Secreted.
Post-translational modification	The N-terminal processed forms of platelet factor 4 variant seems to be produced by proteolytic cleavage. The most abundant form is Platelet factor 4 variant(5-74).
Sequence similarities	Belongs to the intercrine alpha (chemokine CxC) family.
Mass spectrometry	Molecular mass is 8250.9 Da from positions 31 - 104. Determined by ESI. Platelet factor 4 variant. Ref.5 Molecular mass is 7877.7 Da from positions 34 - 104. Determined by ESI. Platelet factor 4 variant(4-74). Ref.5 Molecular mass is 7805.8 Da from positions 35 - 104. Determined by ESI. Platelet factor 4 variant(5-74). Ref.5 Molecular mass is 7678.6 Da from positions 36 - 104. Determined by ESI. Platelet factor 4 variant(6-74). Ref.5

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Heparin-binding
PTM	Disulfide bond Glycoprotein Proteoglycan
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	immune response Inferred from electronic annotation. Source: InterPro leukocyte chemotaxis Inferred from electronic annotation. Source: InterPro platelet activation Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	heparin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Ref.5
	Chain	31 – 104	74	Platelet factor 4 variant		PRO_0000005071
	Chain	34 – 104	71	Platelet factor 4 variant(4-74)		PRO_0000005072
	Chain	35 – 104	70	Platelet factor 4 variant(5-74)		PRO_0000005073
	Chain	36 – 104	69	Platelet factor 4 variant(6-74)		PRO_0000005074
Amino acid modifications
	Disulfide bond	44 ↔ 70		By similarity
	Disulfide bond	46 ↔ 86		By similarity
Experimental info
	Sequence conflict	77	1	A → S no nucleotide entry Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P10720 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 786FE2B288532E28	FASTA	104	11,553
	10 20 30 40 50 60 MSSAARSRLT RATRQEMLFL ALLLLPVVVA FARAEAEEDG DLQCLCVKTT SQVRPRHITS 70 80 90 100 LEVIKAGPHC PTAQLIATLK NGRKICLDLQ ALLYKKIIKE HLES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of PF4var1, a human gene variant of platelet factor 4." Green C.J., St Charles R., Edwards B.F.P., Johnson P.H. Mol. Cell. Biol. 9:1445-1451(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Structural and functional comparison of the genes for human platelet factor 4 and PF4alt." Eisman R., Surrey S., Ramachandran B., Schwartz E., Poncz M. Blood 76:336-344(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 27-104.
[5]	"Platelets release CXCL4L1, a nonallelic variant of the chemokine platelet factor-4/CXCL4 and potent inhibitor of angiogenesis." Struyf S., Burdick M.D., Proost P., Van Damme J., Strieter R.M. Circ. Res. 95:855-857(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 31-54, FUNCTION, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M26167 Genomic DNA. Translation: AAA60067.1. CH471057 Genomic DNA. Translation: EAX05692.1. BC130653 mRNA. Translation: AAI30654.1. BC130657 mRNA. Translation: AAI30658.1.
IPI	IPI00022295.
PIR	PFHU4A. A31941.
RefSeq	NP_002611.1. NM_002620.2.
UniGene	Hs.72933.
3D structure databases
ProteinModelPortal	P10720.
ModBase	Search...
Protein-protein interaction databases
IntAct	P10720. 1 interaction.
STRING	9606.ENSP00000226524.
PTM databases
PhosphoSite	P10720.
Polymorphism databases
DMDM	130306.
Proteomic databases
PaxDb	P10720.
PRIDE	P10720.
Protocols and materials databases
DNASU	5197.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000226524; ENSP00000226524; ENSG00000109272.
GeneID	5197.
KEGG	hsa:5197.
UCSC	uc003hhg.1. human.
Organism-specific databases
CTD	5197.
GeneCards	GC04P074718.
HGNC	HGNC:8862. PF4V1.
HPA	HPA052485.
MIM	173461. gene.
neXtProt	NX_P10720.
PharmGKB	PA33204.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG40449.
HOGENOM	HOG000220915.
HOVERGEN	HBG107789.
InParanoid	P10720.
KO	K05407.
OMA	CLDLQAL.
OrthoDB	EOG44QT2M.
PhylomeDB	P10720.
Gene expression databases
Bgee	P10720.
CleanEx	HS_PF4V1.
Genevestigator	P10720.
GermOnline	ENSG00000109272. Homo sapiens.
Family and domain databases
InterPro	IPR001089. Chemokine_CXC. IPR018048. Chemokine_CXC_CS. IPR001811. Chemokine_IL8-like_dom. IPR027222. PF4. [Graphical view]
PANTHER	PTHR10179. PTHR10179. 1 hit. PTHR10179:SF10. PTHR10179:SF10. 1 hit.
Pfam	PF00048. IL8. 1 hit. [Graphical view]
PRINTS	PR00437. SMALLCYTKCXC.
SMART	SM00199. SCY. 1 hit. [Graphical view]
SUPFAM	SSF54117. Chemokine_IL8. 1 hit.
PROSITE	PS00471. SMALL_CYTOKINES_CXC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	5197.
NextBio	20102.
SOURCE	Search...

Entry information

Entry name

PF4V_HUMAN

Accession

Primary (citable) accession number: P10720
Secondary accession number(s): A1L4S0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 1, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.