Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P10720

- PF4V_HUMAN

UniProt

P10720 - PF4V_HUMAN

Protein

Platelet factor 4 variant

Gene

PF4V1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Inhibitor of angiogenesis. Inhibitor of endothelial cell chemotaxis (in vitro).1 Publication

    GO - Molecular functioni

    1. heparin binding Source: UniProtKB-KW

    GO - Biological processi

    1. cell chemotaxis Source: GOC
    2. immune response Source: InterPro

    Keywords - Molecular functioni

    Cytokine

    Keywords - Ligandi

    Heparin-binding

    Enzyme and pathway databases

    ReactomeiREACT_12051. Cell surface interactions at the vascular wall.
    REACT_1439. Common Pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Platelet factor 4 variant
    Alternative name(s):
    C-X-C motif chemokine 4 variant
    CXCL4L1
    PF4alt
    PF4var1
    Cleaved into the following 3 chains:
    Gene namesi
    Name:PF4V1
    Synonyms:CXCL4V1, SCYB4V1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:8862. PF4V1.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-KW

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33204.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 30301 PublicationAdd
    BLAST
    Chaini31 – 10474Platelet factor 4 variantPRO_0000005071Add
    BLAST
    Chaini34 – 10471Platelet factor 4 variant(4-74)PRO_0000005072Add
    BLAST
    Chaini35 – 10470Platelet factor 4 variant(5-74)PRO_0000005073Add
    BLAST
    Chaini36 – 10469Platelet factor 4 variant(6-74)PRO_0000005074Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi44 ↔ 701 Publication
    Disulfide bondi46 ↔ 861 Publication

    Post-translational modificationi

    The N-terminal processed forms of platelet factor 4 variant seems to be produced by proteolytic cleavage. The most abundant form is Platelet factor 4 variant(5-74).

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Proteoglycan

    Proteomic databases

    PaxDbiP10720.
    PRIDEiP10720.

    PTM databases

    PhosphoSiteiP10720.

    Expressioni

    Gene expression databases

    BgeeiP10720.
    CleanExiHS_PF4V1.
    GenevestigatoriP10720.

    Organism-specific databases

    HPAiHPA052485.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    BioGridi111220. 1 interaction.
    IntActiP10720. 1 interaction.
    STRINGi9606.ENSP00000226524.

    Structurei

    Secondary structure

    1
    104
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi45 – 473
    Turni55 – 573
    Beta strandi58 – 647
    Beta strandi70 – 723
    Beta strandi74 – 796
    Beta strandi84 – 874
    Helixi89 – 968
    Helixi98 – 1014

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4HSVX-ray2.08A/B/C/D35-104[»]
    ProteinModelPortaliP10720.
    SMRiP10720. Positions 40-104.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni95 – 1017Heparin-binding

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG40449.
    HOGENOMiHOG000220915.
    HOVERGENiHBG107789.
    InParanoidiP10720.
    KOiK05407.
    OMAiIVASAHC.
    OrthoDBiEOG7B5WZS.
    PhylomeDBiP10720.
    TreeFamiTF333433.

    Family and domain databases

    InterProiIPR001089. Chemokine_CXC.
    IPR018048. Chemokine_CXC_CS.
    IPR001811. Chemokine_IL8-like_dom.
    [Graphical view]
    PANTHERiPTHR10179. PTHR10179. 1 hit.
    PfamiPF00048. IL8. 1 hit.
    [Graphical view]
    PRINTSiPR00437. SMALLCYTKCXC.
    SMARTiSM00199. SCY. 1 hit.
    [Graphical view]
    SUPFAMiSSF54117. SSF54117. 1 hit.
    PROSITEiPS00471. SMALL_CYTOKINES_CXC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10720-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSAARSRLT RATRQEMLFL ALLLLPVVVA FARAEAEEDG DLQCLCVKTT    50
    SQVRPRHITS LEVIKAGPHC PTAQLIATLK NGRKICLDLQ ALLYKKIIKE 100
    HLES 104
    Length:104
    Mass (Da):11,553
    Last modified:July 1, 1989 - v1
    Checksum:i786FE2B288532E28
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti77 – 771A → S no nucleotide entry (PubMed:1695112)Curated

    Mass spectrometryi

    Molecular mass is 8250.9 Da from positions 31 - 104. Determined by ESI. Platelet factor 4 variant.1 Publication
    Molecular mass is 7877.7 Da from positions 34 - 104. Determined by ESI. Platelet factor 4 variant(4-74).1 Publication
    Molecular mass is 7805.8 Da from positions 35 - 104. Determined by ESI. Platelet factor 4 variant(5-74).1 Publication
    Molecular mass is 7678.6 Da from positions 36 - 104. Determined by ESI. Platelet factor 4 variant(6-74).1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26167 Genomic DNA. Translation: AAA60067.1.
    CH471057 Genomic DNA. Translation: EAX05692.1.
    BC130653 mRNA. Translation: AAI30654.1.
    BC130657 mRNA. Translation: AAI30658.1.
    CCDSiCCDS3561.1.
    PIRiA31941. PFHU4A.
    RefSeqiNP_002611.1. NM_002620.2.
    UniGeneiHs.72933.

    Genome annotation databases

    EnsembliENST00000226524; ENSP00000226524; ENSG00000109272.
    GeneIDi5197.
    KEGGihsa:5197.
    UCSCiuc003hhg.1. human.

    Polymorphism databases

    DMDMi130306.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26167 Genomic DNA. Translation: AAA60067.1 .
    CH471057 Genomic DNA. Translation: EAX05692.1 .
    BC130653 mRNA. Translation: AAI30654.1 .
    BC130657 mRNA. Translation: AAI30658.1 .
    CCDSi CCDS3561.1.
    PIRi A31941. PFHU4A.
    RefSeqi NP_002611.1. NM_002620.2.
    UniGenei Hs.72933.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4HSV X-ray 2.08 A/B/C/D 35-104 [» ]
    ProteinModelPortali P10720.
    SMRi P10720. Positions 40-104.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111220. 1 interaction.
    IntActi P10720. 1 interaction.
    STRINGi 9606.ENSP00000226524.

    PTM databases

    PhosphoSitei P10720.

    Polymorphism databases

    DMDMi 130306.

    Proteomic databases

    PaxDbi P10720.
    PRIDEi P10720.

    Protocols and materials databases

    DNASUi 5197.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000226524 ; ENSP00000226524 ; ENSG00000109272 .
    GeneIDi 5197.
    KEGGi hsa:5197.
    UCSCi uc003hhg.1. human.

    Organism-specific databases

    CTDi 5197.
    GeneCardsi GC04P074718.
    HGNCi HGNC:8862. PF4V1.
    HPAi HPA052485.
    MIMi 173461. gene.
    neXtProti NX_P10720.
    PharmGKBi PA33204.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG40449.
    HOGENOMi HOG000220915.
    HOVERGENi HBG107789.
    InParanoidi P10720.
    KOi K05407.
    OMAi IVASAHC.
    OrthoDBi EOG7B5WZS.
    PhylomeDBi P10720.
    TreeFami TF333433.

    Enzyme and pathway databases

    Reactomei REACT_12051. Cell surface interactions at the vascular wall.
    REACT_1439. Common Pathway.

    Miscellaneous databases

    GenomeRNAii 5197.
    NextBioi 20102.
    PROi P10720.
    SOURCEi Search...

    Gene expression databases

    Bgeei P10720.
    CleanExi HS_PF4V1.
    Genevestigatori P10720.

    Family and domain databases

    InterProi IPR001089. Chemokine_CXC.
    IPR018048. Chemokine_CXC_CS.
    IPR001811. Chemokine_IL8-like_dom.
    [Graphical view ]
    PANTHERi PTHR10179. PTHR10179. 1 hit.
    Pfami PF00048. IL8. 1 hit.
    [Graphical view ]
    PRINTSi PR00437. SMALLCYTKCXC.
    SMARTi SM00199. SCY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54117. SSF54117. 1 hit.
    PROSITEi PS00471. SMALL_CYTOKINES_CXC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of PF4var1, a human gene variant of platelet factor 4."
      Green C.J., St Charles R., Edwards B.F.P., Johnson P.H.
      Mol. Cell. Biol. 9:1445-1451(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "Structural and functional comparison of the genes for human platelet factor 4 and PF4alt."
      Eisman R., Surrey S., Ramachandran B., Schwartz E., Poncz M.
      Blood 76:336-344(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 27-104.
    5. "Platelets release CXCL4L1, a nonallelic variant of the chemokine platelet factor-4/CXCL4 and potent inhibitor of angiogenesis."
      Struyf S., Burdick M.D., Proost P., Van Damme J., Strieter R.M.
      Circ. Res. 95:855-857(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 31-54, FUNCTION, MASS SPECTROMETRY.
    6. "Alternative C-terminal helix orientation alters chemokine function: Structure of the Anti-angiogenic Chemokine, CXCL4L1."
      Kuo J.H., Chen Y.P., Liu J.S., Dubrac A., Quemener C., Prats H., Bikfalvi A., Wu W.G., Sue S.C.
      J. Biol. Chem. 288:13522-13533(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.08 ANGSTROMS) OF 35-104, DISULFIDE BONDS, HEPARIN-BINDING REGION, SUBUNIT.

    Entry informationi

    Entry nameiPF4V_HUMAN
    AccessioniPrimary (citable) accession number: P10720
    Secondary accession number(s): A1L4S0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Binding to heparin is much weaker than in the close homolog PF4/CXCL4.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3