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Protein

Platelet factor 4 variant

Gene

PF4V1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of angiogenesis. Inhibitor of endothelial cell chemotaxis (in vitro).1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiREACT_12051. Cell surface interactions at the vascular wall.
REACT_1439. Common Pathway of Fibrin Clot Formation.

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet factor 4 variant
Alternative name(s):
C-X-C motif chemokine 4 variant
CXCL4L1
PF4alt
PF4var1
Cleaved into the following 3 chains:
Gene namesi
Name:PF4V1
Synonyms:CXCL4V1, SCYB4V1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:8862. PF4V1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33204.

Polymorphism and mutation databases

BioMutaiPF4V1.
DMDMi130306.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 30301 PublicationAdd
BLAST
Chaini31 – 10474Platelet factor 4 variantPRO_0000005071Add
BLAST
Chaini34 – 10471Platelet factor 4 variant(4-74)PRO_0000005072Add
BLAST
Chaini35 – 10470Platelet factor 4 variant(5-74)PRO_0000005073Add
BLAST
Chaini36 – 10469Platelet factor 4 variant(6-74)PRO_0000005074Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 701 Publication
Disulfide bondi46 ↔ 861 Publication

Post-translational modificationi

The N-terminal processed forms of platelet factor 4 variant seems to be produced by proteolytic cleavage. The most abundant form is Platelet factor 4 variant(5-74).

Keywords - PTMi

Disulfide bond, Glycoprotein, Proteoglycan

Proteomic databases

PaxDbiP10720.
PRIDEiP10720.

PTM databases

PhosphoSiteiP10720.

Expressioni

Gene expression databases

BgeeiP10720.
CleanExiHS_PF4V1.
GenevestigatoriP10720.

Organism-specific databases

HPAiHPA052485.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi111220. 1 interaction.
IntActiP10720. 1 interaction.
STRINGi9606.ENSP00000226524.

Structurei

Secondary structure

1
104
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi45 – 473Combined sources
Turni55 – 573Combined sources
Beta strandi58 – 647Combined sources
Beta strandi70 – 723Combined sources
Beta strandi74 – 796Combined sources
Beta strandi84 – 874Combined sources
Helixi89 – 968Combined sources
Helixi98 – 1014Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HSVX-ray2.08A/B/C/D35-104[»]
ProteinModelPortaliP10720.
SMRiP10720. Positions 40-104.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni95 – 1017Heparin-binding

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG40449.
GeneTreeiENSGT00530000062901.
HOGENOMiHOG000220915.
HOVERGENiHBG107789.
InParanoidiP10720.
KOiK05407.
OMAiPEMLFLA.
OrthoDBiEOG7B5WZS.
PhylomeDBiP10720.
TreeFamiTF333433.

Family and domain databases

InterProiIPR001089. Chemokine_CXC.
IPR018048. Chemokine_CXC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PANTHERiPTHR10179. PTHR10179. 1 hit.
PfamiPF00048. IL8. 1 hit.
[Graphical view]
PRINTSiPR00437. SMALLCYTKCXC.
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00471. SMALL_CYTOKINES_CXC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10720-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSAARSRLT RATRQEMLFL ALLLLPVVVA FARAEAEEDG DLQCLCVKTT
60 70 80 90 100
SQVRPRHITS LEVIKAGPHC PTAQLIATLK NGRKICLDLQ ALLYKKIIKE

HLES
Length:104
Mass (Da):11,553
Last modified:July 1, 1989 - v1
Checksum:i786FE2B288532E28
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 771A → S no nucleotide entry (PubMed:1695112).Curated

Mass spectrometryi

Molecular mass is 8250.9 Da from positions 31 - 104. Determined by ESI. Platelet factor 4 variant.1 Publication
Molecular mass is 7877.7 Da from positions 34 - 104. Determined by ESI. Platelet factor 4 variant(4-74).1 Publication
Molecular mass is 7805.8 Da from positions 35 - 104. Determined by ESI. Platelet factor 4 variant(5-74).1 Publication
Molecular mass is 7678.6 Da from positions 36 - 104. Determined by ESI. Platelet factor 4 variant(6-74).1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26167 Genomic DNA. Translation: AAA60067.1.
CH471057 Genomic DNA. Translation: EAX05692.1.
BC130653 mRNA. Translation: AAI30654.1.
BC130657 mRNA. Translation: AAI30658.1.
CCDSiCCDS3561.1.
PIRiA31941. PFHU4A.
RefSeqiNP_002611.1. NM_002620.2.
UniGeneiHs.72933.

Genome annotation databases

EnsembliENST00000226524; ENSP00000226524; ENSG00000109272.
GeneIDi5197.
KEGGihsa:5197.
UCSCiuc003hhg.1. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26167 Genomic DNA. Translation: AAA60067.1.
CH471057 Genomic DNA. Translation: EAX05692.1.
BC130653 mRNA. Translation: AAI30654.1.
BC130657 mRNA. Translation: AAI30658.1.
CCDSiCCDS3561.1.
PIRiA31941. PFHU4A.
RefSeqiNP_002611.1. NM_002620.2.
UniGeneiHs.72933.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HSVX-ray2.08A/B/C/D35-104[»]
ProteinModelPortaliP10720.
SMRiP10720. Positions 40-104.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111220. 1 interaction.
IntActiP10720. 1 interaction.
STRINGi9606.ENSP00000226524.

PTM databases

PhosphoSiteiP10720.

Polymorphism and mutation databases

BioMutaiPF4V1.
DMDMi130306.

Proteomic databases

PaxDbiP10720.
PRIDEiP10720.

Protocols and materials databases

DNASUi5197.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000226524; ENSP00000226524; ENSG00000109272.
GeneIDi5197.
KEGGihsa:5197.
UCSCiuc003hhg.1. human.

Organism-specific databases

CTDi5197.
GeneCardsiGC04P074718.
HGNCiHGNC:8862. PF4V1.
HPAiHPA052485.
MIMi173461. gene.
neXtProtiNX_P10720.
PharmGKBiPA33204.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG40449.
GeneTreeiENSGT00530000062901.
HOGENOMiHOG000220915.
HOVERGENiHBG107789.
InParanoidiP10720.
KOiK05407.
OMAiPEMLFLA.
OrthoDBiEOG7B5WZS.
PhylomeDBiP10720.
TreeFamiTF333433.

Enzyme and pathway databases

ReactomeiREACT_12051. Cell surface interactions at the vascular wall.
REACT_1439. Common Pathway of Fibrin Clot Formation.

Miscellaneous databases

GenomeRNAii5197.
NextBioi20102.
PROiP10720.
SOURCEiSearch...

Gene expression databases

BgeeiP10720.
CleanExiHS_PF4V1.
GenevestigatoriP10720.

Family and domain databases

InterProiIPR001089. Chemokine_CXC.
IPR018048. Chemokine_CXC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PANTHERiPTHR10179. PTHR10179. 1 hit.
PfamiPF00048. IL8. 1 hit.
[Graphical view]
PRINTSiPR00437. SMALLCYTKCXC.
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00471. SMALL_CYTOKINES_CXC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of PF4var1, a human gene variant of platelet factor 4."
    Green C.J., St Charles R., Edwards B.F.P., Johnson P.H.
    Mol. Cell. Biol. 9:1445-1451(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Structural and functional comparison of the genes for human platelet factor 4 and PF4alt."
    Eisman R., Surrey S., Ramachandran B., Schwartz E., Poncz M.
    Blood 76:336-344(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 27-104.
  5. "Platelets release CXCL4L1, a nonallelic variant of the chemokine platelet factor-4/CXCL4 and potent inhibitor of angiogenesis."
    Struyf S., Burdick M.D., Proost P., Van Damme J., Strieter R.M.
    Circ. Res. 95:855-857(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 31-54, FUNCTION, MASS SPECTROMETRY.
  6. "Alternative C-terminal helix orientation alters chemokine function: Structure of the Anti-angiogenic Chemokine, CXCL4L1."
    Kuo J.H., Chen Y.P., Liu J.S., Dubrac A., Quemener C., Prats H., Bikfalvi A., Wu W.G., Sue S.C.
    J. Biol. Chem. 288:13522-13533(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.08 ANGSTROMS) OF 35-104, DISULFIDE BONDS, HEPARIN-BINDING REGION, SUBUNIT.

Entry informationi

Entry nameiPF4V_HUMAN
AccessioniPrimary (citable) accession number: P10720
Secondary accession number(s): A1L4S0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 27, 2015
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Binding to heparin is much weaker than in the close homolog PF4/CXCL4.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.