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P10720 (PF4V_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Platelet factor 4 variant
Alternative name(s):
C-X-C motif chemokine 4 variant
CXCL4L1
PF4alt
PF4var1
Gene names
Name:PF4V1
Synonyms:CXCL4V1, SCYB4V1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length104 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibitor of angiogenesis. Inhibitor of endothelial cell chemotaxis (in vitro). Ref.5

Subunit structure

Homotetramer. Ref.6

Subcellular location

Secreted.

Post-translational modification

The N-terminal processed forms of platelet factor 4 variant seems to be produced by proteolytic cleavage. The most abundant form is Platelet factor 4 variant(5-74).

Miscellaneous

Binding to heparin is much weaker than in the close homolog PF4/CXCL4.

Sequence similarities

Belongs to the intercrine alpha (chemokine CxC) family.

Mass spectrometry

Molecular mass is 8250.9 Da from positions 31 - 104. Determined by ESI. Platelet factor 4 variant. Ref.5

Molecular mass is 7877.7 Da from positions 34 - 104. Determined by ESI. Platelet factor 4 variant(4-74). Ref.5

Molecular mass is 7805.8 Da from positions 35 - 104. Determined by ESI. Platelet factor 4 variant(5-74). Ref.5

Molecular mass is 7678.6 Da from positions 36 - 104. Determined by ESI. Platelet factor 4 variant(6-74). Ref.5

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandHeparin-binding
   Molecular functionCytokine
   PTMDisulfide bond
Glycoprotein
Proteoglycan
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcell chemotaxis

Inferred from electronic annotation. Source: GOC

immune response

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionheparin binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Ref.5
Chain31 – 10474Platelet factor 4 variant
PRO_0000005071
Chain34 – 10471Platelet factor 4 variant(4-74)
PRO_0000005072
Chain35 – 10470Platelet factor 4 variant(5-74)
PRO_0000005073
Chain36 – 10469Platelet factor 4 variant(6-74)
PRO_0000005074

Regions

Region95 – 1017Heparin-binding

Amino acid modifications

Disulfide bond44 ↔ 70 Ref.6
Disulfide bond46 ↔ 86 Ref.6

Experimental info

Sequence conflict771A → S no nucleotide entry Ref.4

Secondary structure

................ 104
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P10720 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 786FE2B288532E28

FASTA10411,553
        10         20         30         40         50         60 
MSSAARSRLT RATRQEMLFL ALLLLPVVVA FARAEAEEDG DLQCLCVKTT SQVRPRHITS 

        70         80         90        100 
LEVIKAGPHC PTAQLIATLK NGRKICLDLQ ALLYKKIIKE HLES 

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterization of PF4var1, a human gene variant of platelet factor 4."
Green C.J., St Charles R., Edwards B.F.P., Johnson P.H.
Mol. Cell. Biol. 9:1445-1451(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Structural and functional comparison of the genes for human platelet factor 4 and PF4alt."
Eisman R., Surrey S., Ramachandran B., Schwartz E., Poncz M.
Blood 76:336-344(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 27-104.
[5]"Platelets release CXCL4L1, a nonallelic variant of the chemokine platelet factor-4/CXCL4 and potent inhibitor of angiogenesis."
Struyf S., Burdick M.D., Proost P., Van Damme J., Strieter R.M.
Circ. Res. 95:855-857(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 31-54, FUNCTION, MASS SPECTROMETRY.
[6]"Alternative C-terminal helix orientation alters chemokine function: Structure of the Anti-angiogenic Chemokine, CXCL4L1."
Kuo J.H., Chen Y.P., Liu J.S., Dubrac A., Quemener C., Prats H., Bikfalvi A., Wu W.G., Sue S.C.
J. Biol. Chem. 288:13522-13533(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.08 ANGSTROMS) OF 35-104, DISULFIDE BONDS, HEPARIN-BINDING REGION, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M26167 Genomic DNA. Translation: AAA60067.1.
CH471057 Genomic DNA. Translation: EAX05692.1.
BC130653 mRNA. Translation: AAI30654.1.
BC130657 mRNA. Translation: AAI30658.1.
CCDSCCDS3561.1.
PIRPFHU4A. A31941.
RefSeqNP_002611.1. NM_002620.2.
UniGeneHs.72933.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4HSVX-ray2.08A/B/C/D35-104[»]
ProteinModelPortalP10720.
SMRP10720. Positions 40-104.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111220. 1 interaction.
IntActP10720. 1 interaction.
STRING9606.ENSP00000226524.

PTM databases

PhosphoSiteP10720.

Polymorphism databases

DMDM130306.

Proteomic databases

PaxDbP10720.
PRIDEP10720.

Protocols and materials databases

DNASU5197.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000226524; ENSP00000226524; ENSG00000109272.
GeneID5197.
KEGGhsa:5197.
UCSCuc003hhg.1. human.

Organism-specific databases

CTD5197.
GeneCardsGC04P074718.
HGNCHGNC:8862. PF4V1.
HPAHPA052485.
MIM173461. gene.
neXtProtNX_P10720.
PharmGKBPA33204.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG40449.
HOGENOMHOG000220915.
HOVERGENHBG107789.
InParanoidP10720.
KOK05407.
OMAIVASAHC.
OrthoDBEOG7B5WZS.
PhylomeDBP10720.
TreeFamTF333433.

Gene expression databases

BgeeP10720.
CleanExHS_PF4V1.
GenevestigatorP10720.

Family and domain databases

InterProIPR001089. Chemokine_CXC.
IPR018048. Chemokine_CXC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PANTHERPTHR10179. PTHR10179. 1 hit.
PfamPF00048. IL8. 1 hit.
[Graphical view]
PRINTSPR00437. SMALLCYTKCXC.
SMARTSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMSSF54117. SSF54117. 1 hit.
PROSITEPS00471. SMALL_CYTOKINES_CXC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi5197.
NextBio20102.
PROP10720.
SOURCESearch...

Entry information

Entry namePF4V_HUMAN
AccessionPrimary (citable) accession number: P10720
Secondary accession number(s): A1L4S0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 9, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM