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Protein

Transcription elongation factor A protein 1

Gene

Tcea1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri259 – 29941TFIIS-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. erythrocyte differentiation Source: MGI
  2. positive regulation of transcription, DNA-templated Source: MGI
  3. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  4. regulation of DNA-templated transcription, elongation Source: InterPro
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_223654. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_224562. Dual incision reaction in TC-NER.
REACT_226490. RNA Polymerase II Transcription Elongation.
REACT_255009. RNA Polymerase II Pre-transcription Events.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor A protein 1
Alternative name(s):
Transcription elongation factor S-II protein 1
Transcription elongation factor TFIIS.o
Gene namesi
Name:Tcea1
Synonyms:Tceat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1196624. Tcea1.

Subcellular locationi

GO - Cellular componenti

  1. nucleolus Source: MGI
  2. nucleoplasm Source: MGI
  3. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301Transcription elongation factor A protein 1PRO_0000121447Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei97 – 971PhosphoserineBy similarity
Modified residuei100 – 1001Phosphoserine2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP10711.
PaxDbiP10711.
PRIDEiP10711.

PTM databases

PhosphoSiteiP10711.

Expressioni

Gene expression databases

BgeeiP10711.
CleanExiMM_TCEA1.
ExpressionAtlasiP10711. baseline and differential.
GenevestigatoriP10711.

Interactioni

Subunit structurei

Associates with UBR5 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulates target gene transcription by recruiting their promoters (By similarity). Interacts with EAF2.By similarity1 Publication

Protein-protein interaction databases

BioGridi203995. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP10711.
SMRiP10711. Positions 1-88, 132-301.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 8078TFIIS N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini140 – 256117TFIIS centralPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TFS-II family.Curated
Contains 1 TFIIS central domain.PROSITE-ProRule annotation
Contains 1 TFIIS N-terminal domain.PROSITE-ProRule annotation
Contains 1 TFIIS-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri259 – 29941TFIIS-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG1594.
HOGENOMiHOG000195015.
HOVERGENiHBG055022.
InParanoidiP10711.
KOiK03145.
OrthoDBiEOG7GQXWQ.
PhylomeDBiP10711.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProiIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR017890. TFS2M.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: P10711-1) [UniParc]FASTAAdd to basket

Also known as: PSII-3

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDEVVRIAK KMDKMVQKKN AAGALDLLKE LKNIPMTLEL LQSTRIGMSV
60 70 80 90 100
NALRKQSTDE EVTSLAKSLI KSWKKLLDGP STDKDPEEKK KEPAISSQNS
110 120 130 140 150
PEAREESSSS SNVSSRKDET NARDTYVSSF PRAPSTSDSV RLKCREMLAA
160 170 180 190 200
ALRTGDDYVA IGADEEELGS QIEEAIYQEI RNTDMKYKNR VRSRISNLKD
210 220 230 240 250
AKNPNLRKNV LCGNIPPDLF ARMTAEEMAS DELKEMRKNL TKEAIREHQM
260 270 280 290 300
AKTGGTQTDL FTCGKCKKKN CTYTQVQTRS ADEPMTTFVV CNECGNRWKF

C
Length:301
Mass (Da):33,880
Last modified:February 12, 2003 - v2
Checksum:i14B5105F8D77DA87
GO
Isoform 1 (identifier: P10711-2) [UniParc]FASTAAdd to basket

Also known as: PSII-2

The sequence of this isoform differs from the canonical sequence as follows:
     1-35: Missing.

Show »
Length:266
Mass (Da):29,930
Checksum:iEFFB2585B81B3F54
GO

Sequence cautioni

The sequence BAA00768.1 differs from that shown.Chimeric cDNA.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3535Missing in isoform 1. 2 PublicationsVSP_006410Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18209 mRNA. Translation: AAA40418.1.
M18210 mRNA. Translation: AAA40419.1.
D00925 mRNA. Translation: BAA00768.1. Sequence problems.
BC006022 mRNA. Translation: AAH06022.1.
BC061490 mRNA. Translation: AAH61490.1.
BC083127 mRNA. Translation: AAH83127.1.
CCDSiCCDS35505.1. [P10711-1]
PIRiA29950.
RefSeqiNP_001153222.1. NM_001159750.1.
NP_001153223.1. NM_001159751.1.
NP_035671.1. NM_011541.4. [P10711-1]
UniGeneiMm.207263.

Genome annotation databases

EnsembliENSMUST00000081551; ENSMUSP00000080266; ENSMUSG00000033813. [P10711-1]
GeneIDi21399.
KEGGimmu:21399.
UCSCiuc007afi.2. mouse. [P10711-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18209 mRNA. Translation: AAA40418.1.
M18210 mRNA. Translation: AAA40419.1.
D00925 mRNA. Translation: BAA00768.1. Sequence problems.
BC006022 mRNA. Translation: AAH06022.1.
BC061490 mRNA. Translation: AAH61490.1.
BC083127 mRNA. Translation: AAH83127.1.
CCDSiCCDS35505.1. [P10711-1]
PIRiA29950.
RefSeqiNP_001153222.1. NM_001159750.1.
NP_001153223.1. NM_001159751.1.
NP_035671.1. NM_011541.4. [P10711-1]
UniGeneiMm.207263.

3D structure databases

ProteinModelPortaliP10711.
SMRiP10711. Positions 1-88, 132-301.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203995. 1 interaction.

PTM databases

PhosphoSiteiP10711.

Proteomic databases

MaxQBiP10711.
PaxDbiP10711.
PRIDEiP10711.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081551; ENSMUSP00000080266; ENSMUSG00000033813. [P10711-1]
GeneIDi21399.
KEGGimmu:21399.
UCSCiuc007afi.2. mouse. [P10711-1]

Organism-specific databases

CTDi6917.
MGIiMGI:1196624. Tcea1.

Phylogenomic databases

eggNOGiCOG1594.
HOGENOMiHOG000195015.
HOVERGENiHBG055022.
InParanoidiP10711.
KOiK03145.
OrthoDBiEOG7GQXWQ.
PhylomeDBiP10711.

Enzyme and pathway databases

ReactomeiREACT_223654. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_224562. Dual incision reaction in TC-NER.
REACT_226490. RNA Polymerase II Transcription Elongation.
REACT_255009. RNA Polymerase II Pre-transcription Events.

Miscellaneous databases

ChiTaRSiTcea1. mouse.
NextBioi300656.
PROiP10711.
SOURCEiSearch...

Gene expression databases

BgeeiP10711.
CleanExiMM_TCEA1.
ExpressionAtlasiP10711. baseline and differential.
GenevestigatoriP10711.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProiIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR017890. TFS2M.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of cDNA for eukaryotic transcription factor S-II."
    Hirashima S., Hirai H., Nakanishi Y., Natori S.
    J. Biol. Chem. 263:3858-3863(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PARTIAL PROTEIN SEQUENCE.
  2. "Heterogeneity and tissue-specific expression of eukaryotic transcription factor S-II-related protein mRNA."
    Kanai A., Kuzuhara T., Sekimizu K., Natori S.
    J. Biochem. 109:674-677(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver and Mammary gland.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: Czech II and FVB/N.
    Tissue: Mammary gland and Olfactory epithelium.
  4. "Identification of a novel tissue-specific transcriptional activator FESTA as a protein that interacts with the transcription elongation factor S-II."
    Saso K., Ito T., Natori S., Sekimizu K.
    J. Biochem. 133:493-500(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EAF2.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
    Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
    J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiTCEA1_MOUSE
AccessioniPrimary (citable) accession number: P10711
Secondary accession number(s): P10712, P23713
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 12, 2003
Last modified: March 4, 2015
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

S-II binds to RNA-polymerase II in the absence of transcription.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.