Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription elongation factor A protein 1

Gene

Tcea1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

Miscellaneous

S-II binds to RNA-polymerase II in the absence of transcription.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri259 – 299TFIIS-typePROSITE-ProRule annotationAdd BLAST41

GO - Molecular functioni

GO - Biological processi

  • erythrocyte differentiation Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-112382. Formation of RNA Pol II elongation complex.
R-MMU-674695. RNA Polymerase II Pre-transcription Events.
R-MMU-6781823. Formation of TC-NER Pre-Incision Complex.
R-MMU-6782135. Dual incision in TC-NER.
R-MMU-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-MMU-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-MMU-75955. RNA Polymerase II Transcription Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor A protein 1
Alternative name(s):
Transcription elongation factor S-II protein 1
Transcription elongation factor TFIIS.o
Gene namesi
Name:Tcea1
Synonyms:Tceat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1196624. Tcea1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001214471 – 301Transcription elongation factor A protein 1Add BLAST301

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Cross-linki55Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei57PhosphoserineBy similarity1
Modified residuei81PhosphoserineBy similarity1
Modified residuei97PhosphoserineCombined sources1
Modified residuei100PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP10711.
PaxDbiP10711.
PeptideAtlasiP10711.
PRIDEiP10711.

PTM databases

iPTMnetiP10711.
PhosphoSitePlusiP10711.

Expressioni

Gene expression databases

BgeeiENSMUSG00000033813.
CleanExiMM_TCEA1.
ExpressionAtlasiP10711. baseline and differential.
GenevisibleiP10711. MM.

Interactioni

Subunit structurei

Associates with UBR5 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulates target gene transcription by recruiting their promoters (By similarity). Interacts with EAF2.By similarity1 Publication

Protein-protein interaction databases

BioGridi203995. 1 interactor.
IntActiP10711. 1 interactor.
STRINGi10090.ENSMUSP00000129157.

Structurei

3D structure databases

ProteinModelPortaliP10711.
SMRiP10711.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 80TFIIS N-terminalPROSITE-ProRule annotationAdd BLAST78
Domaini140 – 256TFIIS centralPROSITE-ProRule annotationAdd BLAST117

Sequence similaritiesi

Belongs to the TFS-II family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri259 – 299TFIIS-typePROSITE-ProRule annotationAdd BLAST41

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1105. Eukaryota.
COG1594. LUCA.
GeneTreeiENSGT00390000017794.
HOGENOMiHOG000195015.
HOVERGENiHBG055022.
InParanoidiP10711.
KOiK03145.
PhylomeDBiP10711.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
InterProiView protein in InterPro
IPR035100. TF_IIS-typ.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
PfamiView protein in Pfam
PF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiView protein in SMART
SM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiView protein in PROSITE
PS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: P10711-1) [UniParc]FASTAAdd to basket
Also known as: PSII-3

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDEVVRIAK KMDKMVQKKN AAGALDLLKE LKNIPMTLEL LQSTRIGMSV
60 70 80 90 100
NALRKQSTDE EVTSLAKSLI KSWKKLLDGP STDKDPEEKK KEPAISSQNS
110 120 130 140 150
PEAREESSSS SNVSSRKDET NARDTYVSSF PRAPSTSDSV RLKCREMLAA
160 170 180 190 200
ALRTGDDYVA IGADEEELGS QIEEAIYQEI RNTDMKYKNR VRSRISNLKD
210 220 230 240 250
AKNPNLRKNV LCGNIPPDLF ARMTAEEMAS DELKEMRKNL TKEAIREHQM
260 270 280 290 300
AKTGGTQTDL FTCGKCKKKN CTYTQVQTRS ADEPMTTFVV CNECGNRWKF

C
Length:301
Mass (Da):33,880
Last modified:February 12, 2003 - v2
Checksum:i14B5105F8D77DA87
GO
Isoform 1 (identifier: P10711-2) [UniParc]FASTAAdd to basket
Also known as: PSII-2

The sequence of this isoform differs from the canonical sequence as follows:
     1-35: Missing.

Show »
Length:266
Mass (Da):29,930
Checksum:iEFFB2585B81B3F54
GO

Sequence cautioni

The sequence BAA00768 differs from that shown. Chimeric cDNA.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0064101 – 35Missing in isoform 1. 2 PublicationsAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18209 mRNA. Translation: AAA40418.1.
M18210 mRNA. Translation: AAA40419.1.
D00925 mRNA. Translation: BAA00768.1. Sequence problems.
BC006022 mRNA. Translation: AAH06022.1.
BC061490 mRNA. Translation: AAH61490.1.
BC083127 mRNA. Translation: AAH83127.1.
CCDSiCCDS35505.1. [P10711-1]
PIRiA29950.
RefSeqiNP_001153222.1. NM_001159750.1.
NP_001153223.1. NM_001159751.1.
NP_035671.1. NM_011541.4. [P10711-1]
UniGeneiMm.207263.

Genome annotation databases

EnsembliENSMUST00000081551; ENSMUSP00000080266; ENSMUSG00000033813. [P10711-1]
GeneIDi21399.
KEGGimmu:21399.
UCSCiuc007afi.2. mouse. [P10711-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTCEA1_MOUSE
AccessioniPrimary (citable) accession number: P10711
Secondary accession number(s): P10712, P23713
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 12, 2003
Last modified: September 27, 2017
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families