Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P10711

- TCEA1_MOUSE

UniProt

P10711 - TCEA1_MOUSE

Protein

Transcription elongation factor A protein 1

Gene

Tcea1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (12 Feb 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri259 – 29941TFIIS-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. erythrocyte differentiation Source: MGI
    2. positive regulation of transcription, DNA-templated Source: MGI
    3. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    4. regulation of DNA-templated transcription, elongation Source: InterPro
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_223654. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_224562. Dual incision reaction in TC-NER.
    REACT_226490. RNA Polymerase II Transcription Elongation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription elongation factor A protein 1
    Alternative name(s):
    Transcription elongation factor S-II protein 1
    Transcription elongation factor TFIIS.o
    Gene namesi
    Name:Tcea1
    Synonyms:Tceat
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1196624. Tcea1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleoplasm Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 301301Transcription elongation factor A protein 1PRO_0000121447Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei97 – 971PhosphoserineBy similarity
    Modified residuei100 – 1001Phosphoserine2 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP10711.
    PaxDbiP10711.
    PRIDEiP10711.

    PTM databases

    PhosphoSiteiP10711.

    Expressioni

    Gene expression databases

    BgeeiP10711.
    CleanExiMM_TCEA1.
    GenevestigatoriP10711.

    Interactioni

    Subunit structurei

    Associates with UBR5 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulates target gene transcription by recruiting their promoters By similarity. Interacts with EAF2.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi203995. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP10711.
    SMRiP10711. Positions 1-88, 132-301.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 8078TFIIS N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini140 – 256117TFIIS centralPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TFS-II family.Curated
    Contains 1 TFIIS central domain.PROSITE-ProRule annotation
    Contains 1 TFIIS N-terminal domain.PROSITE-ProRule annotation
    Contains 1 TFIIS-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri259 – 29941TFIIS-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG1594.
    HOGENOMiHOG000195015.
    HOVERGENiHBG055022.
    InParanoidiP10711.
    KOiK03145.
    OrthoDBiEOG7GQXWQ.
    PhylomeDBiP10711.

    Family and domain databases

    Gene3Di1.10.472.30. 1 hit.
    1.20.930.10. 1 hit.
    InterProiIPR016492. TF_IIS-rel.
    IPR003617. TFIIS/CRSP70_N_sub.
    IPR003618. TFIIS_cen_dom.
    IPR017923. TFIIS_N.
    IPR017890. TFS2M.
    IPR006289. TFSII.
    IPR001222. Znf_TFIIS.
    [Graphical view]
    PfamiPF08711. Med26. 1 hit.
    PF01096. TFIIS_C. 1 hit.
    PF07500. TFIIS_M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006704. TF_IIS. 1 hit.
    SMARTiSM00510. TFS2M. 1 hit.
    SM00509. TFS2N. 1 hit.
    SM00440. ZnF_C2C2. 1 hit.
    [Graphical view]
    SUPFAMiSSF46942. SSF46942. 1 hit.
    SSF47676. SSF47676. 1 hit.
    TIGRFAMsiTIGR01385. TFSII. 1 hit.
    PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
    PS51319. TFIIS_N. 1 hit.
    PS00466. ZF_TFIIS_1. 1 hit.
    PS51133. ZF_TFIIS_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 2 (identifier: P10711-1) [UniParc]FASTAAdd to Basket

    Also known as: PSII-3

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEDEVVRIAK KMDKMVQKKN AAGALDLLKE LKNIPMTLEL LQSTRIGMSV    50
    NALRKQSTDE EVTSLAKSLI KSWKKLLDGP STDKDPEEKK KEPAISSQNS 100
    PEAREESSSS SNVSSRKDET NARDTYVSSF PRAPSTSDSV RLKCREMLAA 150
    ALRTGDDYVA IGADEEELGS QIEEAIYQEI RNTDMKYKNR VRSRISNLKD 200
    AKNPNLRKNV LCGNIPPDLF ARMTAEEMAS DELKEMRKNL TKEAIREHQM 250
    AKTGGTQTDL FTCGKCKKKN CTYTQVQTRS ADEPMTTFVV CNECGNRWKF 300
    C 301
    Length:301
    Mass (Da):33,880
    Last modified:February 12, 2003 - v2
    Checksum:i14B5105F8D77DA87
    GO
    Isoform 1 (identifier: P10711-2) [UniParc]FASTAAdd to Basket

    Also known as: PSII-2

    The sequence of this isoform differs from the canonical sequence as follows:
         1-35: Missing.

    Show »
    Length:266
    Mass (Da):29,930
    Checksum:iEFFB2585B81B3F54
    GO

    Sequence cautioni

    The sequence BAA00768.1 differs from that shown. Reason: Chimeric cDNA.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 3535Missing in isoform 1. 2 PublicationsVSP_006410Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M18209 mRNA. Translation: AAA40418.1.
    M18210 mRNA. Translation: AAA40419.1.
    D00925 mRNA. Translation: BAA00768.1. Sequence problems.
    BC006022 mRNA. Translation: AAH06022.1.
    BC061490 mRNA. Translation: AAH61490.1.
    BC083127 mRNA. Translation: AAH83127.1.
    CCDSiCCDS35505.1. [P10711-1]
    PIRiA29950.
    RefSeqiNP_001153222.1. NM_001159750.1.
    NP_001153223.1. NM_001159751.1.
    NP_035671.1. NM_011541.4. [P10711-1]
    UniGeneiMm.207263.

    Genome annotation databases

    EnsembliENSMUST00000081551; ENSMUSP00000080266; ENSMUSG00000033813. [P10711-1]
    GeneIDi21399.
    KEGGimmu:21399.
    UCSCiuc007afi.2. mouse. [P10711-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M18209 mRNA. Translation: AAA40418.1 .
    M18210 mRNA. Translation: AAA40419.1 .
    D00925 mRNA. Translation: BAA00768.1 . Sequence problems.
    BC006022 mRNA. Translation: AAH06022.1 .
    BC061490 mRNA. Translation: AAH61490.1 .
    BC083127 mRNA. Translation: AAH83127.1 .
    CCDSi CCDS35505.1. [P10711-1 ]
    PIRi A29950.
    RefSeqi NP_001153222.1. NM_001159750.1.
    NP_001153223.1. NM_001159751.1.
    NP_035671.1. NM_011541.4. [P10711-1 ]
    UniGenei Mm.207263.

    3D structure databases

    ProteinModelPortali P10711.
    SMRi P10711. Positions 1-88, 132-301.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203995. 1 interaction.

    PTM databases

    PhosphoSitei P10711.

    Proteomic databases

    MaxQBi P10711.
    PaxDbi P10711.
    PRIDEi P10711.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000081551 ; ENSMUSP00000080266 ; ENSMUSG00000033813 . [P10711-1 ]
    GeneIDi 21399.
    KEGGi mmu:21399.
    UCSCi uc007afi.2. mouse. [P10711-1 ]

    Organism-specific databases

    CTDi 6917.
    MGIi MGI:1196624. Tcea1.

    Phylogenomic databases

    eggNOGi COG1594.
    HOGENOMi HOG000195015.
    HOVERGENi HBG055022.
    InParanoidi P10711.
    KOi K03145.
    OrthoDBi EOG7GQXWQ.
    PhylomeDBi P10711.

    Enzyme and pathway databases

    Reactomei REACT_223654. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_224562. Dual incision reaction in TC-NER.
    REACT_226490. RNA Polymerase II Transcription Elongation.

    Miscellaneous databases

    ChiTaRSi TCEA1. mouse.
    NextBioi 300656.
    PROi P10711.
    SOURCEi Search...

    Gene expression databases

    Bgeei P10711.
    CleanExi MM_TCEA1.
    Genevestigatori P10711.

    Family and domain databases

    Gene3Di 1.10.472.30. 1 hit.
    1.20.930.10. 1 hit.
    InterProi IPR016492. TF_IIS-rel.
    IPR003617. TFIIS/CRSP70_N_sub.
    IPR003618. TFIIS_cen_dom.
    IPR017923. TFIIS_N.
    IPR017890. TFS2M.
    IPR006289. TFSII.
    IPR001222. Znf_TFIIS.
    [Graphical view ]
    Pfami PF08711. Med26. 1 hit.
    PF01096. TFIIS_C. 1 hit.
    PF07500. TFIIS_M. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006704. TF_IIS. 1 hit.
    SMARTi SM00510. TFS2M. 1 hit.
    SM00509. TFS2N. 1 hit.
    SM00440. ZnF_C2C2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46942. SSF46942. 1 hit.
    SSF47676. SSF47676. 1 hit.
    TIGRFAMsi TIGR01385. TFSII. 1 hit.
    PROSITEi PS51321. TFIIS_CENTRAL. 1 hit.
    PS51319. TFIIS_N. 1 hit.
    PS00466. ZF_TFIIS_1. 1 hit.
    PS51133. ZF_TFIIS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of cDNA for eukaryotic transcription factor S-II."
      Hirashima S., Hirai H., Nakanishi Y., Natori S.
      J. Biol. Chem. 263:3858-3863(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PARTIAL PROTEIN SEQUENCE.
    2. "Heterogeneity and tissue-specific expression of eukaryotic transcription factor S-II-related protein mRNA."
      Kanai A., Kuzuhara T., Sekimizu K., Natori S.
      J. Biochem. 109:674-677(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver and Mammary gland.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: Czech II and FVB/N.
      Tissue: Mammary gland and Olfactory epithelium.
    4. "Identification of a novel tissue-specific transcriptional activator FESTA as a protein that interacts with the transcription elongation factor S-II."
      Saso K., Ito T., Natori S., Sekimizu K.
      J. Biochem. 133:493-500(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH EAF2.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
      Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
      J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiTCEA1_MOUSE
    AccessioniPrimary (citable) accession number: P10711
    Secondary accession number(s): P10712, P23713
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: February 12, 2003
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    S-II binds to RNA-polymerase II in the absence of transcription.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3