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Protein

Alkaline phosphatase, placental-like

Gene

ALPPL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61MagnesiumBy similarity1
Metal bindingi61Zinc 1By similarity1
Active sitei111Phosphoserine intermediate1
Metal bindingi111Zinc 1By similarity1
Metal bindingi174MagnesiumBy similarity1
Metal bindingi330MagnesiumBy similarity1
Metal bindingi335Zinc 2By similarity1
Metal bindingi339Zinc 2; via tele nitrogenBy similarity1
Metal bindingi376Zinc 1By similarity1
Metal bindingi377Zinc 1; via tele nitrogenBy similarity1
Metal bindingi451Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

  • alkaline phosphatase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS08820-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase, placental-like (EC:3.1.3.1)
Alternative name(s):
ALP-1
Alkaline phosphatase Nagao isozyme
Germ cell alkaline phosphatase
Short name:
GCAP
Placental alkaline phosphatase-like
Short name:
PLAP-like
Gene namesi
Name:ALPPL2
Synonyms:ALPPL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:441. ALPPL2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi251.
OpenTargetsiENSG00000163286.
PharmGKBiPA24731.

Chemistry databases

ChEMBLiCHEMBL3402.
DrugBankiDB01143. Amifostine.

Polymorphism and mutation databases

BioMutaiALPPL2.
DMDMi145559564.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000002403320 – 503Alkaline phosphatase, placental-likeAdd BLAST484
PropeptideiPRO_0000024034504 – 532Removed in mature formBy similarityAdd BLAST29

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi140 ↔ 202By similarity
Glycosylationi141N-linked (GlcNAc...)Sequence analysis1
Glycosylationi268N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi486 ↔ 493By similarity
Lipidationi503GPI-anchor amidated aspartateBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiP10696.
PaxDbiP10696.
PeptideAtlasiP10696.
PRIDEiP10696.

PTM databases

DEPODiP10696.
iPTMnetiP10696.
PhosphoSitePlusiP10696.

Expressioni

Tissue specificityi

Trace amounts in the testis and thymus, and in elevated amounts in germ cell tumors.

Gene expression databases

BgeeiENSG00000163286.
CleanExiHS_ALPPL2.
GenevisibleiP10696. HS.

Organism-specific databases

HPAiCAB020698.
HPA038764.
HPA038765.
HPA051699.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi106752. 9 interactors.
IntActiP10696. 2 interactors.
STRINGi9606.ENSP00000295453.

Chemistry databases

BindingDBiP10696.

Structurei

3D structure databases

ProteinModelPortaliP10696.
SMRiP10696.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP10696.
KOiK01077.
OMAiAWEGPKA.
OrthoDBiEOG091G067H.
PhylomeDBiP10696.
TreeFamiTF323513.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQGPWVLLLL GLRLQLSLGI IPVEEENPDF WNRQAAEALG AAKKLQPAQT
60 70 80 90 100
AAKNLIIFLG DGMGVSTVTA ARILKGQKKD KLGPETFLAM DRFPYVALSK
110 120 130 140 150
TYSVDKHVPD SGATATAYLC GVKGNFQTIG LSAAARFNQC NTTRGNEVIS
160 170 180 190 200
VMNRAKKAGK SVGVVTTTRV QHASPAGAYA HTVNRNWYSD ADVPASARQE
210 220 230 240 250
GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPDDY SQGGTRLDGK
260 270 280 290 300
NLVQEWLAKH QGARYVWNRT ELLQASLDPS VTHLMGLFEP GDMKYEIHRD
310 320 330 340 350
STLDPSLMEM TEAALLLLSR NPRGFFLFVE GGRIDHGHHE SRAYRALTET
360 370 380 390 400
IMFDDAIERA GQLTSEEDTL SLVTADHSHV FSFGGYPLRG SSIFGLAPGK
410 420 430 440 450
ARDRKAYTVL LYGNGPGYVL KDGARPDVTE SESGSPEYRQ QSAVPLDGET
460 470 480 490 500
HAGEDVAVFA RGPQAHLVHG VQEQTFIAHV MAFAACLEPY TACDLAPRAG
510 520 530
TTDAAHPGPS VVPALLPLLA GTLLLLGTAT AP
Length:532
Mass (Da):57,377
Last modified:March 6, 2007 - v4
Checksum:i25EB56C901B61505
GO

Sequence cautioni

The sequence CAA30232 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57I → M in AAA98616 (PubMed:2834730).Curated1
Sequence conflicti152M → V in AAA98616 (PubMed:2834730).Curated1
Sequence conflicti152M → V in CAA39425 (PubMed:2162249).Curated1
Sequence conflicti178A → T in AAA98616 (PubMed:2834730).Curated1
Sequence conflicti178A → T in AAA51700 (PubMed:2745460).Curated1
Sequence conflicti178A → T in CAA39425 (PubMed:2162249).Curated1
Sequence conflicti260H → R in AAH14139 (PubMed:15489334).Curated1
Sequence conflicti380V → L in AAA51700 (PubMed:2745460).Curated1
Sequence conflicti498R → P in AAA98616 (PubMed:2834730).Curated1
Sequence conflicti498R → P in CAA39425 (PubMed:2162249).Curated1
Sequence conflicti498R → S in CAA37374 (PubMed:2297757).Curated1
Sequence conflicti531A → T in CAA37374 (PubMed:2297757).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02755234Q → E.Corresponds to variant rs1048983dbSNPEnsembl.1
Natural variantiVAR_027553273L → M.2 PublicationsCorresponds to variant rs17416141dbSNPEnsembl.1
Natural variantiVAR_027554316L → R.5 PublicationsCorresponds to variant rs1048992dbSNPEnsembl.1
Natural variantiVAR_027555527G → E.Corresponds to variant rs1048999dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03252 Genomic DNA. Translation: AAA98616.1.
J04948 mRNA. Translation: AAA51700.1.
X53279 mRNA. Translation: CAA37374.1.
X55958 mRNA. Translation: CAA39425.1.
AK293017 mRNA. Translation: BAF85706.1.
AC068134 Genomic DNA. Translation: AAY24088.1.
CH471063 Genomic DNA. Translation: EAW70994.1.
BC014139 mRNA. Translation: AAH14139.1.
M19160 Genomic DNA. Translation: AAA51707.1.
X07247 Genomic DNA. Translation: CAA30232.1. Sequence problems.
CCDSiCCDS2491.1.
PIRiS12076.
RefSeqiNP_112603.2. NM_031313.2.
UniGeneiHs.333509.

Genome annotation databases

EnsembliENST00000295453; ENSP00000295453; ENSG00000163286.
GeneIDi251.
KEGGihsa:251.
UCSCiuc002vss.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03252 Genomic DNA. Translation: AAA98616.1.
J04948 mRNA. Translation: AAA51700.1.
X53279 mRNA. Translation: CAA37374.1.
X55958 mRNA. Translation: CAA39425.1.
AK293017 mRNA. Translation: BAF85706.1.
AC068134 Genomic DNA. Translation: AAY24088.1.
CH471063 Genomic DNA. Translation: EAW70994.1.
BC014139 mRNA. Translation: AAH14139.1.
M19160 Genomic DNA. Translation: AAA51707.1.
X07247 Genomic DNA. Translation: CAA30232.1. Sequence problems.
CCDSiCCDS2491.1.
PIRiS12076.
RefSeqiNP_112603.2. NM_031313.2.
UniGeneiHs.333509.

3D structure databases

ProteinModelPortaliP10696.
SMRiP10696.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106752. 9 interactors.
IntActiP10696. 2 interactors.
STRINGi9606.ENSP00000295453.

Chemistry databases

BindingDBiP10696.
ChEMBLiCHEMBL3402.
DrugBankiDB01143. Amifostine.

PTM databases

DEPODiP10696.
iPTMnetiP10696.
PhosphoSitePlusiP10696.

Polymorphism and mutation databases

BioMutaiALPPL2.
DMDMi145559564.

Proteomic databases

MaxQBiP10696.
PaxDbiP10696.
PeptideAtlasiP10696.
PRIDEiP10696.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000295453; ENSP00000295453; ENSG00000163286.
GeneIDi251.
KEGGihsa:251.
UCSCiuc002vss.5. human.

Organism-specific databases

CTDi251.
DisGeNETi251.
GeneCardsiALPPL2.
HGNCiHGNC:441. ALPPL2.
HPAiCAB020698.
HPA038764.
HPA038765.
HPA051699.
MIMi171810. gene.
neXtProtiNX_P10696.
OpenTargetsiENSG00000163286.
PharmGKBiPA24731.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP10696.
KOiK01077.
OMAiAWEGPKA.
OrthoDBiEOG091G067H.
PhylomeDBiP10696.
TreeFamiTF323513.

Enzyme and pathway databases

BioCyciZFISH:HS08820-MONOMER.

Miscellaneous databases

GenomeRNAii251.
PROiP10696.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000163286.
CleanExiHS_ALPPL2.
GenevisibleiP10696. HS.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPBN_HUMAN
AccessioniPrimary (citable) accession number: P10696
Secondary accession number(s): A8KAF2
, Q16727, Q53S81, Q96CM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: March 6, 2007
Last modified: November 30, 2016
This is version 167 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.