P10696 (PPBN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alkaline phosphatase, placental-like
EC=3.1.3.1

Alternative name(s):
ALP-1
Alkaline phosphatase Nagao isozyme
Germ cell alkaline phosphatase
Short name=GCAP
Placental alkaline phosphatase-like
Short name=PLAP-like

Gene names

Name:	ALPPL2
Synonyms:	ALPPL

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion By similarity. Binds 2 zinc ions By similarity.
Subunit structure	Homodimer.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Tissue specificity	Trace amounts in the testis and thymus, and in elevated amounts in germ cell tumors.
Miscellaneous	In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).
Sequence similarities	Belongs to the alkaline phosphatase family.
Sequence caution	The sequence CAA30232.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Signal
Ligand	Magnesium Metal-binding Zinc
Molecular function	Hydrolase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphorylation Non-traceable author statement. Source: UniProtKB
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane Non-traceable author statement. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	alkaline phosphatase activity Non-traceable author statement Ref.4. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 503	484	Alkaline phosphatase, placental-like		PRO_0000024033
	Propeptide	504 – 532	29	Removed in mature form By similarity		PRO_0000024034
Sites
	Active site	111	1	Phosphoserine intermediate
	Metal binding	61	1	Magnesium Potential
	Metal binding	61	1	Zinc 2 Potential
	Metal binding	330	1	Magnesium Potential
	Metal binding	335	1	Zinc 1 Potential
	Metal binding	339	1	Zinc 1 Potential
	Metal binding	376	1	Zinc 2 Potential
	Metal binding	377	1	Zinc 2 Potential
	Metal binding	451	1	Zinc 1 Potential
Amino acid modifications
	Lipidation	503	1	GPI-anchor amidated aspartate By similarity
	Glycosylation	141	1	N-linked (GlcNAc...) Potential
	Glycosylation	268	1	N-linked (GlcNAc...) Potential
	Disulfide bond	140 ↔ 202		By similarity
	Disulfide bond	486 ↔ 493		By similarity
Natural variations
	Natural variant	34	1	Q → E. Corresponds to variant rs1048983 [ dbSNP | Ensembl ].		VAR_027552
	Natural variant	273	1	L → M. Ref.3 Ref.8 Corresponds to variant rs17416141 [ dbSNP | Ensembl ].		VAR_027553
	Natural variant	316	1	L → R. Ref.3 Ref.4 Ref.5 Ref.7 Ref.8 Corresponds to variant rs1048992 [ dbSNP | Ensembl ].		VAR_027554
	Natural variant	527	1	G → E. Corresponds to variant rs1048999 [ dbSNP | Ensembl ].		VAR_027555
Experimental info
	Sequence conflict	57	1	I → M in AAA98616. Ref.1
	Sequence conflict	152	1	M → V in AAA98616. Ref.1
	Sequence conflict	152	1	M → V in CAA39425. Ref.4
	Sequence conflict	178	1	A → T in AAA98616. Ref.1
	Sequence conflict	178	1	A → T in AAA51700. Ref.2
	Sequence conflict	178	1	A → T in CAA39425. Ref.4
	Sequence conflict	260	1	H → R in AAH14139. Ref.8
	Sequence conflict	380	1	V → L in AAA51700. Ref.2
	Sequence conflict	498	1	R → P in AAA98616. Ref.1
	Sequence conflict	498	1	R → P in CAA39425. Ref.4
	Sequence conflict	498	1	R → S in CAA37374. Ref.3
	Sequence conflict	531	1	A → T in CAA37374. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P10696 [UniParc]. Last modified March 6, 2007. Version 4. Checksum: 25EB56C901B61505	FASTA	532	57,377
	10 20 30 40 50 60 MQGPWVLLLL GLRLQLSLGI IPVEEENPDF WNRQAAEALG AAKKLQPAQT AAKNLIIFLG 70 80 90 100 110 120 DGMGVSTVTA ARILKGQKKD KLGPETFLAM DRFPYVALSK TYSVDKHVPD SGATATAYLC 130 140 150 160 170 180 GVKGNFQTIG LSAAARFNQC NTTRGNEVIS VMNRAKKAGK SVGVVTTTRV QHASPAGAYA 190 200 210 220 230 240 HTVNRNWYSD ADVPASARQE GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPDDY 250 260 270 280 290 300 SQGGTRLDGK NLVQEWLAKH QGARYVWNRT ELLQASLDPS VTHLMGLFEP GDMKYEIHRD 310 320 330 340 350 360 STLDPSLMEM TEAALLLLSR NPRGFFLFVE GGRIDHGHHE SRAYRALTET IMFDDAIERA 370 380 390 400 410 420 GQLTSEEDTL SLVTADHSHV FSFGGYPLRG SSIFGLAPGK ARDRKAYTVL LYGNGPGYVL 430 440 450 460 470 480 KDGARPDVTE SESGSPEYRQ QSAVPLDGET HAGEDVAVFA RGPQAHLVHG VQEQTFIAHV 490 500 510 520 530 MAFAACLEPY TACDLAPRAG TTDAAHPGPS VVPALLPLLA GTLLLLGTAT AP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Seminoma-derived Nagao isozyme is encoded by a germ-cell alkaline phosphatase gene." Millan J.L., Manes T. Proc. Natl. Acad. Sci. U.S.A. 85:3024-3028(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Expression of the germ cell alkaline phosphatase gene in human choriocarcinoma cells." Watanabe S., Watanabe T., Li W.L., Soong B.-W., Chou J.Y. J. Biol. Chem. 264:12611-12619(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Choriocarcinoma.
[3]	"Molecular cloning of complementary DNAs encoding alkaline phosphatase in human colon cancer cells." Gum J.R. Jr., Hicks J.W., Sack T.L., Kim Y.S. Cancer Res. 50:1085-1091(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS MET-273 AND ARG-316. Tissue: Colon.
[4]	"Expression of a Nagao-type, phosphatidylinositol-glycan anchored alkaline phosphatase in human choriocarcinomas." Lowe M.E., Strauss A.W. Cancer Res. 50:3956-3962(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-316.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-316. Tissue: Uterus.
[6]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ARG-316.
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS MET-273 AND ARG-316. Tissue: Placenta.
[9]	"Two gene duplication events in the evolution of the human heat-stable alkaline phosphatases." Knoll B.J., Rothblum K.N., Longley M. Gene 60:267-276(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
[10]	"5' nucleotide sequence of a putative human placental alkaline phosphatase-like gene." Shen L.P., Liu H., Kan Y.W., Kam W. Nucleic Acids Res. 16:5694-5694(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-157.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J03252 Genomic DNA. Translation: AAA98616.1. J04948 mRNA. Translation: AAA51700.1. X53279 mRNA. Translation: CAA37374.1. X55958 mRNA. Translation: CAA39425.1. AK293017 mRNA. Translation: BAF85706.1. AC068134 Genomic DNA. Translation: AAY24088.1. CH471063 Genomic DNA. Translation: EAW70994.1. BC014139 mRNA. Translation: AAH14139.1. M19160 Genomic DNA. Translation: AAA51707.1. X07247 Genomic DNA. Translation: CAA30232.1. Sequence problems.
IPI	IPI00290380.
PIR	S12076.
RefSeq	NP_112603.2. NM_031313.2.
UniGene	Hs.333509.
3D structure databases
ProteinModelPortal	P10696.
ModBase	Search...
Protein-protein interaction databases
IntAct	P10696. 2 interactions.
STRING	9606.ENSP00000295453.
PTM databases
PhosphoSite	P10696.
Polymorphism databases
DMDM	145559564.
Proteomic databases
PaxDb	P10696.
PRIDE	P10696.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000295453; ENSP00000295453; ENSG00000163286.
GeneID	251.
KEGG	hsa:251.
UCSC	uc002vss.4. human.
Organism-specific databases
CTD	251.
GeneCards	GC02P233273.
HGNC	HGNC:441. ALPPL2.
HPA	CAB020698.
MIM	171810. gene.
neXtProt	NX_P10696.
PharmGKB	PA24731.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1785.
HOGENOM	HOG000099118.
HOVERGEN	HBG007345.
InParanoid	P10696.
KO	K01077.
OMA	CPSNALE.
OrthoDB	EOG4K3KW1.
PhylomeDB	P10696.
Gene expression databases
Bgee	P10696.
CleanEx	HS_ALPPL2.
Genevestigator	P10696.
GermOnline	ENSG00000163286. Homo sapiens.
Family and domain databases
Gene3D	3.40.720.10. 1 hit.
InterPro	IPR017849. Alkaline_Pase-like_a/b/a. IPR001952. Alkaline_phosphatase. IPR018299. Alkaline_phosphatase_AS. IPR017850. Alkaline_phosphatase_core. [Graphical view]
Pfam	PF00245. Alk_phosphatase. 1 hit. [Graphical view]
PRINTS	PR00113. ALKPHPHTASE.
SMART	SM00098. alkPPc. 1 hit. [Graphical view]
SUPFAM	SSF53649. Alkaline_phosphatase_core. 1 hit.
PROSITE	PS00123. ALKALINE_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P10696.
ChEMBL	CHEMBL3402.
DrugBank	DB01143. Amifostine. DB00848. Levamisole.
GenomeRNAi	251.
NextBio	1005.
SOURCE	Search...

Entry information

Entry name

PPBN_HUMAN

Accession

Primary (citable) accession number: P10696
Secondary accession number(s): A8KAF2 , Q16727, Q53S81, Q96CM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	March 6, 2007
Last modified:	May 1, 2013
This is version 138 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.