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P10696

- PPBN_HUMAN

UniProt

P10696 - PPBN_HUMAN

Protein

Alkaline phosphatase, placental-like

Gene

ALPPL2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 149 (01 Oct 2014)
      Sequence version 4 (06 Mar 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion.By similarity
    Binds 2 zinc ions.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi61 – 611MagnesiumBy similarity
    Metal bindingi61 – 611Zinc 1By similarity
    Active sitei111 – 1111Phosphoserine intermediate
    Metal bindingi111 – 1111Zinc 1By similarity
    Metal bindingi174 – 1741MagnesiumBy similarity
    Metal bindingi330 – 3301MagnesiumBy similarity
    Metal bindingi335 – 3351Zinc 2By similarity
    Metal bindingi339 – 3391Zinc 2; via tele nitrogenBy similarity
    Metal bindingi376 – 3761Zinc 1By similarity
    Metal bindingi377 – 3771Zinc 1; via tele nitrogenBy similarity
    Metal bindingi451 – 4511Zinc 2; via tele nitrogenBy similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. dephosphorylation Source: GOC

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase, placental-like (EC:3.1.3.1)
    Alternative name(s):
    ALP-1
    Alkaline phosphatase Nagao isozyme
    Germ cell alkaline phosphatase
    Short name:
    GCAP
    Placental alkaline phosphatase-like
    Short name:
    PLAP-like
    Gene namesi
    Name:ALPPL2
    Synonyms:ALPPL
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:441. ALPPL2.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24731.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 503484Alkaline phosphatase, placental-likePRO_0000024033Add
    BLAST
    Propeptidei504 – 53229Removed in mature formBy similarityPRO_0000024034Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi140 ↔ 202By similarity
    Glycosylationi141 – 1411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi268 – 2681N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi486 ↔ 493By similarity
    Lipidationi503 – 5031GPI-anchor amidated aspartateBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP10696.
    PaxDbiP10696.
    PRIDEiP10696.

    PTM databases

    PhosphoSiteiP10696.

    Expressioni

    Tissue specificityi

    Trace amounts in the testis and thymus, and in elevated amounts in germ cell tumors.

    Gene expression databases

    BgeeiP10696.
    CleanExiHS_ALPPL2.
    GenevestigatoriP10696.

    Organism-specific databases

    HPAiCAB020698.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi106752. 3 interactions.
    IntActiP10696. 2 interactions.
    STRINGi9606.ENSP00000295453.

    Structurei

    3D structure databases

    ProteinModelPortaliP10696.
    SMRiP10696. Positions 20-500.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1785.
    HOGENOMiHOG000099118.
    HOVERGENiHBG007345.
    InParanoidiP10696.
    KOiK01077.
    OMAiDKGFFLQ.
    OrthoDBiEOG7SN8CH.
    PhylomeDBiP10696.
    TreeFamiTF323513.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10696-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQGPWVLLLL GLRLQLSLGI IPVEEENPDF WNRQAAEALG AAKKLQPAQT    50
    AAKNLIIFLG DGMGVSTVTA ARILKGQKKD KLGPETFLAM DRFPYVALSK 100
    TYSVDKHVPD SGATATAYLC GVKGNFQTIG LSAAARFNQC NTTRGNEVIS 150
    VMNRAKKAGK SVGVVTTTRV QHASPAGAYA HTVNRNWYSD ADVPASARQE 200
    GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPDDY SQGGTRLDGK 250
    NLVQEWLAKH QGARYVWNRT ELLQASLDPS VTHLMGLFEP GDMKYEIHRD 300
    STLDPSLMEM TEAALLLLSR NPRGFFLFVE GGRIDHGHHE SRAYRALTET 350
    IMFDDAIERA GQLTSEEDTL SLVTADHSHV FSFGGYPLRG SSIFGLAPGK 400
    ARDRKAYTVL LYGNGPGYVL KDGARPDVTE SESGSPEYRQ QSAVPLDGET 450
    HAGEDVAVFA RGPQAHLVHG VQEQTFIAHV MAFAACLEPY TACDLAPRAG 500
    TTDAAHPGPS VVPALLPLLA GTLLLLGTAT AP 532
    Length:532
    Mass (Da):57,377
    Last modified:March 6, 2007 - v4
    Checksum:i25EB56C901B61505
    GO

    Sequence cautioni

    The sequence CAA30232.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti57 – 571I → M in AAA98616. (PubMed:2834730)Curated
    Sequence conflicti152 – 1521M → V in AAA98616. (PubMed:2834730)Curated
    Sequence conflicti152 – 1521M → V in CAA39425. (PubMed:2162249)Curated
    Sequence conflicti178 – 1781A → T in AAA98616. (PubMed:2834730)Curated
    Sequence conflicti178 – 1781A → T in AAA51700. (PubMed:2745460)Curated
    Sequence conflicti178 – 1781A → T in CAA39425. (PubMed:2162249)Curated
    Sequence conflicti260 – 2601H → R in AAH14139. (PubMed:15489334)Curated
    Sequence conflicti380 – 3801V → L in AAA51700. (PubMed:2745460)Curated
    Sequence conflicti498 – 4981R → P in AAA98616. (PubMed:2834730)Curated
    Sequence conflicti498 – 4981R → P in CAA39425. (PubMed:2162249)Curated
    Sequence conflicti498 – 4981R → S in CAA37374. (PubMed:2297757)Curated
    Sequence conflicti531 – 5311A → T in CAA37374. (PubMed:2297757)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti34 – 341Q → E.
    Corresponds to variant rs1048983 [ dbSNP | Ensembl ].
    VAR_027552
    Natural varianti273 – 2731L → M.2 Publications
    Corresponds to variant rs17416141 [ dbSNP | Ensembl ].
    VAR_027553
    Natural varianti316 – 3161L → R.5 Publications
    Corresponds to variant rs1048992 [ dbSNP | Ensembl ].
    VAR_027554
    Natural varianti527 – 5271G → E.
    Corresponds to variant rs1048999 [ dbSNP | Ensembl ].
    VAR_027555

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03252 Genomic DNA. Translation: AAA98616.1.
    J04948 mRNA. Translation: AAA51700.1.
    X53279 mRNA. Translation: CAA37374.1.
    X55958 mRNA. Translation: CAA39425.1.
    AK293017 mRNA. Translation: BAF85706.1.
    AC068134 Genomic DNA. Translation: AAY24088.1.
    CH471063 Genomic DNA. Translation: EAW70994.1.
    BC014139 mRNA. Translation: AAH14139.1.
    M19160 Genomic DNA. Translation: AAA51707.1.
    X07247 Genomic DNA. Translation: CAA30232.1. Sequence problems.
    CCDSiCCDS2491.1.
    PIRiS12076.
    RefSeqiNP_112603.2. NM_031313.2.
    UniGeneiHs.333509.

    Genome annotation databases

    EnsembliENST00000295453; ENSP00000295453; ENSG00000163286.
    GeneIDi251.
    KEGGihsa:251.
    UCSCiuc002vss.4. human.

    Polymorphism databases

    DMDMi145559564.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03252 Genomic DNA. Translation: AAA98616.1 .
    J04948 mRNA. Translation: AAA51700.1 .
    X53279 mRNA. Translation: CAA37374.1 .
    X55958 mRNA. Translation: CAA39425.1 .
    AK293017 mRNA. Translation: BAF85706.1 .
    AC068134 Genomic DNA. Translation: AAY24088.1 .
    CH471063 Genomic DNA. Translation: EAW70994.1 .
    BC014139 mRNA. Translation: AAH14139.1 .
    M19160 Genomic DNA. Translation: AAA51707.1 .
    X07247 Genomic DNA. Translation: CAA30232.1 . Sequence problems.
    CCDSi CCDS2491.1.
    PIRi S12076.
    RefSeqi NP_112603.2. NM_031313.2.
    UniGenei Hs.333509.

    3D structure databases

    ProteinModelPortali P10696.
    SMRi P10696. Positions 20-500.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 106752. 3 interactions.
    IntActi P10696. 2 interactions.
    STRINGi 9606.ENSP00000295453.

    Chemistry

    BindingDBi P10696.
    ChEMBLi CHEMBL3402.
    DrugBanki DB01143. Amifostine.
    DB00848. Levamisole.

    PTM databases

    PhosphoSitei P10696.

    Polymorphism databases

    DMDMi 145559564.

    Proteomic databases

    MaxQBi P10696.
    PaxDbi P10696.
    PRIDEi P10696.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000295453 ; ENSP00000295453 ; ENSG00000163286 .
    GeneIDi 251.
    KEGGi hsa:251.
    UCSCi uc002vss.4. human.

    Organism-specific databases

    CTDi 251.
    GeneCardsi GC02P233273.
    HGNCi HGNC:441. ALPPL2.
    HPAi CAB020698.
    MIMi 171810. gene.
    neXtProti NX_P10696.
    PharmGKBi PA24731.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1785.
    HOGENOMi HOG000099118.
    HOVERGENi HBG007345.
    InParanoidi P10696.
    KOi K01077.
    OMAi DKGFFLQ.
    OrthoDBi EOG7SN8CH.
    PhylomeDBi P10696.
    TreeFami TF323513.

    Miscellaneous databases

    GenomeRNAii 251.
    NextBioi 1005.
    PROi P10696.
    SOURCEi Search...

    Gene expression databases

    Bgeei P10696.
    CleanExi HS_ALPPL2.
    Genevestigatori P10696.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Seminoma-derived Nagao isozyme is encoded by a germ-cell alkaline phosphatase gene."
      Millan J.L., Manes T.
      Proc. Natl. Acad. Sci. U.S.A. 85:3024-3028(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Expression of the germ cell alkaline phosphatase gene in human choriocarcinoma cells."
      Watanabe S., Watanabe T., Li W.L., Soong B.-W., Chou J.Y.
      J. Biol. Chem. 264:12611-12619(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Choriocarcinoma.
    3. "Molecular cloning of complementary DNAs encoding alkaline phosphatase in human colon cancer cells."
      Gum J.R. Jr., Hicks J.W., Sack T.L., Kim Y.S.
      Cancer Res. 50:1085-1091(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS MET-273 AND ARG-316.
      Tissue: Colon.
    4. "Expression of a Nagao-type, phosphatidylinositol-glycan anchored alkaline phosphatase in human choriocarcinomas."
      Lowe M.E., Strauss A.W.
      Cancer Res. 50:3956-3962(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-316.
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-316.
      Tissue: Uterus.
    6. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ARG-316.
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS MET-273 AND ARG-316.
      Tissue: Placenta.
    9. "Two gene duplication events in the evolution of the human heat-stable alkaline phosphatases."
      Knoll B.J., Rothblum K.N., Longley M.
      Gene 60:267-276(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
    10. "5' nucleotide sequence of a putative human placental alkaline phosphatase-like gene."
      Shen L.P., Liu H., Kan Y.W., Kam W.
      Nucleic Acids Res. 16:5694-5694(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-157.

    Entry informationi

    Entry nameiPPBN_HUMAN
    AccessioniPrimary (citable) accession number: P10696
    Secondary accession number(s): A8KAF2
    , Q16727, Q53S81, Q96CM1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: March 6, 2007
    Last modified: October 1, 2014
    This is version 149 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3