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Protein

Alkaline phosphatase, placental-like

Gene

ALPPL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611MagnesiumBy similarity
Metal bindingi61 – 611Zinc 1By similarity
Active sitei111 – 1111Phosphoserine intermediate
Metal bindingi111 – 1111Zinc 1By similarity
Metal bindingi174 – 1741MagnesiumBy similarity
Metal bindingi330 – 3301MagnesiumBy similarity
Metal bindingi335 – 3351Zinc 2By similarity
Metal bindingi339 – 3391Zinc 2; via tele nitrogenBy similarity
Metal bindingi376 – 3761Zinc 1By similarity
Metal bindingi377 – 3771Zinc 1; via tele nitrogenBy similarity
Metal bindingi451 – 4511Zinc 2; via tele nitrogenBy similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase, placental-like (EC:3.1.3.1)
Alternative name(s):
ALP-1
Alkaline phosphatase Nagao isozyme
Germ cell alkaline phosphatase
Short name:
GCAP
Placental alkaline phosphatase-like
Short name:
PLAP-like
Gene namesi
Name:ALPPL2
Synonyms:ALPPL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:441. ALPPL2.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24731.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 503484Alkaline phosphatase, placental-likePRO_0000024033Add
BLAST
Propeptidei504 – 53229Removed in mature formBy similarityPRO_0000024034Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi140 ↔ 202By similarity
Glycosylationi141 – 1411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi268 – 2681N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi486 ↔ 493By similarity
Lipidationi503 – 5031GPI-anchor amidated aspartateBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

MaxQBiP10696.
PaxDbiP10696.
PRIDEiP10696.

PTM databases

DEPODiP10696.
PhosphoSiteiP10696.

Expressioni

Tissue specificityi

Trace amounts in the testis and thymus, and in elevated amounts in germ cell tumors.

Gene expression databases

BgeeiP10696.
CleanExiHS_ALPPL2.
GenevestigatoriP10696.

Organism-specific databases

HPAiCAB020698.
HPA038764.
HPA038765.
HPA051699.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi106752. 3 interactions.
IntActiP10696. 2 interactions.
STRINGi9606.ENSP00000295453.

Structurei

3D structure databases

ProteinModelPortaliP10696.
SMRiP10696. Positions 20-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1785.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP10696.
KOiK01077.
OMAiCPSNALE.
OrthoDBiEOG7SN8CH.
PhylomeDBiP10696.
TreeFamiTF323513.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQGPWVLLLL GLRLQLSLGI IPVEEENPDF WNRQAAEALG AAKKLQPAQT
60 70 80 90 100
AAKNLIIFLG DGMGVSTVTA ARILKGQKKD KLGPETFLAM DRFPYVALSK
110 120 130 140 150
TYSVDKHVPD SGATATAYLC GVKGNFQTIG LSAAARFNQC NTTRGNEVIS
160 170 180 190 200
VMNRAKKAGK SVGVVTTTRV QHASPAGAYA HTVNRNWYSD ADVPASARQE
210 220 230 240 250
GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPDDY SQGGTRLDGK
260 270 280 290 300
NLVQEWLAKH QGARYVWNRT ELLQASLDPS VTHLMGLFEP GDMKYEIHRD
310 320 330 340 350
STLDPSLMEM TEAALLLLSR NPRGFFLFVE GGRIDHGHHE SRAYRALTET
360 370 380 390 400
IMFDDAIERA GQLTSEEDTL SLVTADHSHV FSFGGYPLRG SSIFGLAPGK
410 420 430 440 450
ARDRKAYTVL LYGNGPGYVL KDGARPDVTE SESGSPEYRQ QSAVPLDGET
460 470 480 490 500
HAGEDVAVFA RGPQAHLVHG VQEQTFIAHV MAFAACLEPY TACDLAPRAG
510 520 530
TTDAAHPGPS VVPALLPLLA GTLLLLGTAT AP
Length:532
Mass (Da):57,377
Last modified:March 5, 2007 - v4
Checksum:i25EB56C901B61505
GO

Sequence cautioni

The sequence CAA30232.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571I → M in AAA98616 (PubMed:2834730).Curated
Sequence conflicti152 – 1521M → V in AAA98616 (PubMed:2834730).Curated
Sequence conflicti152 – 1521M → V in CAA39425 (PubMed:2162249).Curated
Sequence conflicti178 – 1781A → T in AAA98616 (PubMed:2834730).Curated
Sequence conflicti178 – 1781A → T in AAA51700 (PubMed:2745460).Curated
Sequence conflicti178 – 1781A → T in CAA39425 (PubMed:2162249).Curated
Sequence conflicti260 – 2601H → R in AAH14139 (PubMed:15489334).Curated
Sequence conflicti380 – 3801V → L in AAA51700 (PubMed:2745460).Curated
Sequence conflicti498 – 4981R → P in AAA98616 (PubMed:2834730).Curated
Sequence conflicti498 – 4981R → P in CAA39425 (PubMed:2162249).Curated
Sequence conflicti498 – 4981R → S in CAA37374 (PubMed:2297757).Curated
Sequence conflicti531 – 5311A → T in CAA37374 (PubMed:2297757).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti34 – 341Q → E.
Corresponds to variant rs1048983 [ dbSNP | Ensembl ].
VAR_027552
Natural varianti273 – 2731L → M.2 Publications
Corresponds to variant rs17416141 [ dbSNP | Ensembl ].
VAR_027553
Natural varianti316 – 3161L → R.5 Publications
Corresponds to variant rs1048992 [ dbSNP | Ensembl ].
VAR_027554
Natural varianti527 – 5271G → E.
Corresponds to variant rs1048999 [ dbSNP | Ensembl ].
VAR_027555

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03252 Genomic DNA. Translation: AAA98616.1.
J04948 mRNA. Translation: AAA51700.1.
X53279 mRNA. Translation: CAA37374.1.
X55958 mRNA. Translation: CAA39425.1.
AK293017 mRNA. Translation: BAF85706.1.
AC068134 Genomic DNA. Translation: AAY24088.1.
CH471063 Genomic DNA. Translation: EAW70994.1.
BC014139 mRNA. Translation: AAH14139.1.
M19160 Genomic DNA. Translation: AAA51707.1.
X07247 Genomic DNA. Translation: CAA30232.1. Sequence problems.
CCDSiCCDS2491.1.
PIRiS12076.
RefSeqiNP_112603.2. NM_031313.2.
UniGeneiHs.333509.

Genome annotation databases

EnsembliENST00000295453; ENSP00000295453; ENSG00000163286.
GeneIDi251.
KEGGihsa:251.
UCSCiuc002vss.4. human.

Polymorphism databases

DMDMi145559564.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03252 Genomic DNA. Translation: AAA98616.1.
J04948 mRNA. Translation: AAA51700.1.
X53279 mRNA. Translation: CAA37374.1.
X55958 mRNA. Translation: CAA39425.1.
AK293017 mRNA. Translation: BAF85706.1.
AC068134 Genomic DNA. Translation: AAY24088.1.
CH471063 Genomic DNA. Translation: EAW70994.1.
BC014139 mRNA. Translation: AAH14139.1.
M19160 Genomic DNA. Translation: AAA51707.1.
X07247 Genomic DNA. Translation: CAA30232.1. Sequence problems.
CCDSiCCDS2491.1.
PIRiS12076.
RefSeqiNP_112603.2. NM_031313.2.
UniGeneiHs.333509.

3D structure databases

ProteinModelPortaliP10696.
SMRiP10696. Positions 20-500.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106752. 3 interactions.
IntActiP10696. 2 interactions.
STRINGi9606.ENSP00000295453.

Chemistry

BindingDBiP10696.
ChEMBLiCHEMBL3402.
DrugBankiDB01143. Amifostine.

PTM databases

DEPODiP10696.
PhosphoSiteiP10696.

Polymorphism databases

DMDMi145559564.

Proteomic databases

MaxQBiP10696.
PaxDbiP10696.
PRIDEiP10696.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000295453; ENSP00000295453; ENSG00000163286.
GeneIDi251.
KEGGihsa:251.
UCSCiuc002vss.4. human.

Organism-specific databases

CTDi251.
GeneCardsiGC02P233273.
HGNCiHGNC:441. ALPPL2.
HPAiCAB020698.
HPA038764.
HPA038765.
HPA051699.
MIMi171810. gene.
neXtProtiNX_P10696.
PharmGKBiPA24731.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1785.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP10696.
KOiK01077.
OMAiCPSNALE.
OrthoDBiEOG7SN8CH.
PhylomeDBiP10696.
TreeFamiTF323513.

Miscellaneous databases

GenomeRNAii251.
NextBioi1005.
PROiP10696.
SOURCEiSearch...

Gene expression databases

BgeeiP10696.
CleanExiHS_ALPPL2.
GenevestigatoriP10696.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Seminoma-derived Nagao isozyme is encoded by a germ-cell alkaline phosphatase gene."
    Millan J.L., Manes T.
    Proc. Natl. Acad. Sci. U.S.A. 85:3024-3028(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Expression of the germ cell alkaline phosphatase gene in human choriocarcinoma cells."
    Watanabe S., Watanabe T., Li W.L., Soong B.-W., Chou J.Y.
    J. Biol. Chem. 264:12611-12619(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Choriocarcinoma.
  3. "Molecular cloning of complementary DNAs encoding alkaline phosphatase in human colon cancer cells."
    Gum J.R. Jr., Hicks J.W., Sack T.L., Kim Y.S.
    Cancer Res. 50:1085-1091(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS MET-273 AND ARG-316.
    Tissue: Colon.
  4. "Expression of a Nagao-type, phosphatidylinositol-glycan anchored alkaline phosphatase in human choriocarcinomas."
    Lowe M.E., Strauss A.W.
    Cancer Res. 50:3956-3962(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-316.
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-316.
    Tissue: Uterus.
  6. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ARG-316.
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS MET-273 AND ARG-316.
    Tissue: Placenta.
  9. "Two gene duplication events in the evolution of the human heat-stable alkaline phosphatases."
    Knoll B.J., Rothblum K.N., Longley M.
    Gene 60:267-276(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
  10. "5' nucleotide sequence of a putative human placental alkaline phosphatase-like gene."
    Shen L.P., Liu H., Kan Y.W., Kam W.
    Nucleic Acids Res. 16:5694-5694(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-157.

Entry informationi

Entry nameiPPBN_HUMAN
AccessioniPrimary (citable) accession number: P10696
Secondary accession number(s): A8KAF2
, Q16727, Q53S81, Q96CM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 31, 1990
Last sequence update: March 5, 2007
Last modified: March 3, 2015
This is version 153 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.