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P10696 (PPBN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 146. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alkaline phosphatase, placental-like

EC=3.1.3.1
Alternative name(s):
ALP-1
Alkaline phosphatase Nagao isozyme
Germ cell alkaline phosphatase
Short name=GCAP
Placental alkaline phosphatase-like
Short name=PLAP-like
Gene names
Name:ALPPL2
Synonyms:ALPPL
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length532 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion By similarity.

Binds 2 zinc ions By similarity.

Subunit structure

Homodimer.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor.

Tissue specificity

Trace amounts in the testis and thymus, and in elevated amounts in germ cell tumors.

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Sequence similarities

Belongs to the alkaline phosphatase family.

Sequence caution

The sequence CAA30232.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityPolymorphism
   DomainSignal
   LigandMagnesium
Metal-binding
Zinc
   Molecular functionHydrolase
   PTMDisulfide bond
Glycoprotein
GPI-anchor
Lipoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdephosphorylation

Non-traceable author statement Ref.4. Source: GOC

   Cellular_componentanchored component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionalkaline phosphatase activity

Non-traceable author statement Ref.4. Source: UniProtKB

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 503484Alkaline phosphatase, placental-like
PRO_0000024033
Propeptide504 – 53229Removed in mature form By similarity
PRO_0000024034

Sites

Active site1111Phosphoserine intermediate
Metal binding611Magnesium By similarity
Metal binding611Zinc 1 By similarity
Metal binding1111Zinc 1 By similarity
Metal binding1741Magnesium By similarity
Metal binding3301Magnesium By similarity
Metal binding3351Zinc 2 By similarity
Metal binding3391Zinc 2; via tele nitrogen By similarity
Metal binding3761Zinc 1 By similarity
Metal binding3771Zinc 1; via tele nitrogen By similarity
Metal binding4511Zinc 2; via tele nitrogen By similarity

Amino acid modifications

Lipidation5031GPI-anchor amidated aspartate By similarity
Glycosylation1411N-linked (GlcNAc...) Potential
Glycosylation2681N-linked (GlcNAc...) Potential
Disulfide bond140 ↔ 202 By similarity
Disulfide bond486 ↔ 493 By similarity

Natural variations

Natural variant341Q → E.
Corresponds to variant rs1048983 [ dbSNP | Ensembl ].
VAR_027552
Natural variant2731L → M. Ref.3 Ref.8
Corresponds to variant rs17416141 [ dbSNP | Ensembl ].
VAR_027553
Natural variant3161L → R. Ref.3 Ref.4 Ref.5 Ref.7 Ref.8
Corresponds to variant rs1048992 [ dbSNP | Ensembl ].
VAR_027554
Natural variant5271G → E.
Corresponds to variant rs1048999 [ dbSNP | Ensembl ].
VAR_027555

Experimental info

Sequence conflict571I → M in AAA98616. Ref.1
Sequence conflict1521M → V in AAA98616. Ref.1
Sequence conflict1521M → V in CAA39425. Ref.4
Sequence conflict1781A → T in AAA98616. Ref.1
Sequence conflict1781A → T in AAA51700. Ref.2
Sequence conflict1781A → T in CAA39425. Ref.4
Sequence conflict2601H → R in AAH14139. Ref.8
Sequence conflict3801V → L in AAA51700. Ref.2
Sequence conflict4981R → P in AAA98616. Ref.1
Sequence conflict4981R → P in CAA39425. Ref.4
Sequence conflict4981R → S in CAA37374. Ref.3
Sequence conflict5311A → T in CAA37374. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P10696 [UniParc].

Last modified March 6, 2007. Version 4.
Checksum: 25EB56C901B61505

FASTA53257,377
        10         20         30         40         50         60 
MQGPWVLLLL GLRLQLSLGI IPVEEENPDF WNRQAAEALG AAKKLQPAQT AAKNLIIFLG 

        70         80         90        100        110        120 
DGMGVSTVTA ARILKGQKKD KLGPETFLAM DRFPYVALSK TYSVDKHVPD SGATATAYLC 

       130        140        150        160        170        180 
GVKGNFQTIG LSAAARFNQC NTTRGNEVIS VMNRAKKAGK SVGVVTTTRV QHASPAGAYA 

       190        200        210        220        230        240 
HTVNRNWYSD ADVPASARQE GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPDDY 

       250        260        270        280        290        300 
SQGGTRLDGK NLVQEWLAKH QGARYVWNRT ELLQASLDPS VTHLMGLFEP GDMKYEIHRD 

       310        320        330        340        350        360 
STLDPSLMEM TEAALLLLSR NPRGFFLFVE GGRIDHGHHE SRAYRALTET IMFDDAIERA 

       370        380        390        400        410        420 
GQLTSEEDTL SLVTADHSHV FSFGGYPLRG SSIFGLAPGK ARDRKAYTVL LYGNGPGYVL 

       430        440        450        460        470        480 
KDGARPDVTE SESGSPEYRQ QSAVPLDGET HAGEDVAVFA RGPQAHLVHG VQEQTFIAHV 

       490        500        510        520        530 
MAFAACLEPY TACDLAPRAG TTDAAHPGPS VVPALLPLLA GTLLLLGTAT AP 

« Hide

References

« Hide 'large scale' references
[1]"Seminoma-derived Nagao isozyme is encoded by a germ-cell alkaline phosphatase gene."
Millan J.L., Manes T.
Proc. Natl. Acad. Sci. U.S.A. 85:3024-3028(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Expression of the germ cell alkaline phosphatase gene in human choriocarcinoma cells."
Watanabe S., Watanabe T., Li W.L., Soong B.-W., Chou J.Y.
J. Biol. Chem. 264:12611-12619(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Choriocarcinoma.
[3]"Molecular cloning of complementary DNAs encoding alkaline phosphatase in human colon cancer cells."
Gum J.R. Jr., Hicks J.W., Sack T.L., Kim Y.S.
Cancer Res. 50:1085-1091(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS MET-273 AND ARG-316.
Tissue: Colon.
[4]"Expression of a Nagao-type, phosphatidylinositol-glycan anchored alkaline phosphatase in human choriocarcinomas."
Lowe M.E., Strauss A.W.
Cancer Res. 50:3956-3962(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-316.
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-316.
Tissue: Uterus.
[6]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ARG-316.
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS MET-273 AND ARG-316.
Tissue: Placenta.
[9]"Two gene duplication events in the evolution of the human heat-stable alkaline phosphatases."
Knoll B.J., Rothblum K.N., Longley M.
Gene 60:267-276(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
[10]"5' nucleotide sequence of a putative human placental alkaline phosphatase-like gene."
Shen L.P., Liu H., Kan Y.W., Kam W.
Nucleic Acids Res. 16:5694-5694(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-157.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J03252 Genomic DNA. Translation: AAA98616.1.
J04948 mRNA. Translation: AAA51700.1.
X53279 mRNA. Translation: CAA37374.1.
X55958 mRNA. Translation: CAA39425.1.
AK293017 mRNA. Translation: BAF85706.1.
AC068134 Genomic DNA. Translation: AAY24088.1.
CH471063 Genomic DNA. Translation: EAW70994.1.
BC014139 mRNA. Translation: AAH14139.1.
M19160 Genomic DNA. Translation: AAA51707.1.
X07247 Genomic DNA. Translation: CAA30232.1. Sequence problems.
PIRS12076.
RefSeqNP_112603.2. NM_031313.2.
UniGeneHs.333509.

3D structure databases

ProteinModelPortalP10696.
SMRP10696. Positions 20-500.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid106752. 3 interactions.
IntActP10696. 2 interactions.
STRING9606.ENSP00000295453.

Chemistry

BindingDBP10696.
ChEMBLCHEMBL3402.
DrugBankDB01143. Amifostine.
DB00848. Levamisole.

PTM databases

PhosphoSiteP10696.

Polymorphism databases

DMDM145559564.

Proteomic databases

PaxDbP10696.
PRIDEP10696.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000295453; ENSP00000295453; ENSG00000163286.
GeneID251.
KEGGhsa:251.
UCSCuc002vss.4. human.

Organism-specific databases

CTD251.
GeneCardsGC02P233273.
HGNCHGNC:441. ALPPL2.
HPACAB020698.
MIM171810. gene.
neXtProtNX_P10696.
PharmGKBPA24731.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1785.
HOGENOMHOG000099118.
HOVERGENHBG007345.
InParanoidP10696.
KOK01077.
OMARWEGPKA.
OrthoDBEOG7SN8CH.
PhylomeDBP10696.
TreeFamTF323513.

Gene expression databases

BgeeP10696.
CleanExHS_ALPPL2.
GenevestigatorP10696.

Family and domain databases

Gene3D3.40.720.10. 1 hit.
InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00113. ALKPHPHTASE.
SMARTSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMSSF53649. SSF53649. 1 hit.
PROSITEPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi251.
NextBio1005.
PROP10696.
SOURCESearch...

Entry information

Entry namePPBN_HUMAN
AccessionPrimary (citable) accession number: P10696
Secondary accession number(s): A8KAF2 expand/collapse secondary AC list , Q16727, Q53S81, Q96CM1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: March 6, 2007
Last modified: April 16, 2014
This is version 146 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM