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P10664

- RL4A_YEAST

UniProt

P10664 - RL4A_YEAST

Protein

60S ribosomal protein L4-A

Gene

RPL4A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Participates in the regulation of the accumulation of its own mRNA.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. RNA binding Source: UniProtKB-KW
    3. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29009-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60S ribosomal protein L4-A
    Alternative name(s):
    L2
    RP2
    YL2
    Gene namesi
    Name:RPL4A
    Synonyms:RPL2, RPL2A
    Ordered Locus Names:YBR031W
    ORF Names:YBR0315
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    SGDiS000000235. RPL4A.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosolic large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 36236160S ribosomal protein L4-APRO_0000129367Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Post-translational modificationi

    N-terminally acetylated by acetyltransferase NatA.1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP10664.
    PeptideAtlasiP10664.
    PRIDEiP10664.

    Expressioni

    Gene expression databases

    GenevestigatoriP10664.

    Interactioni

    Subunit structurei

    Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    EGD2P388792EBI-15390,EBI-6379

    Protein-protein interaction databases

    BioGridi32733. 190 interactions.
    IntActiP10664. 217 interactions.
    MINTiMINT-1325639.
    STRINGi4932.YBR031W.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K5Ymodel-D5-260[»]
    1VW8electron microscopy6.10D1-362[»]
    1VWUelectron microscopy6.10D1-362[»]
    3IZSelectron microscopy-D1-362[»]
    3J65electron microscopy8.70D1-362[»]
    3O58X-ray4.00D1-362[»]
    3O5HX-ray4.00D1-362[»]
    3U5EX-ray3.00C1-362[»]
    3U5IX-ray3.00C1-362[»]
    4B6Aelectron microscopy8.10C1-362[»]
    4BYNelectron microscopy4.30C2-362[»]
    4BYUelectron microscopy6.60C2-362[»]
    4CUWelectron microscopy3.70C1-362[»]
    ProteinModelPortaliP10664.
    SMRiP10664. Positions 2-362.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L4P family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00390000018145.
    HOGENOMiHOG000107331.
    KOiK02930.
    OMAiVASNENK.
    OrthoDBiEOG7NSBC8.

    Family and domain databases

    Gene3Di3.40.1370.10. 1 hit.
    InterProiIPR025755. Ribos_L4_C_dom.
    IPR002136. Ribosomal_L4/L1e.
    IPR013000. Ribosomal_L4/L1e_euk/arc_CS.
    IPR023574. Ribosomal_L4_dom.
    [Graphical view]
    PfamiPF14374. Ribos_L4_asso_C. 1 hit.
    PF00573. Ribosomal_L4. 1 hit.
    [Graphical view]
    SUPFAMiSSF52166. SSF52166. 1 hit.
    PROSITEiPS00939. RIBOSOMAL_L1E. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10664-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRPQVTVHS LTGEATANAL PLPAVFSAPI RPDIVHTVFT SVNKNKRQAY    50
    AVSEKAGHQT SAESWGTGRA VARIPRVGGG GTGRSGQGAF GNMCRGGRMF 100
    APTKTWRKWN VKVNHNEKRY ATASAIAATA VASLVLARGH RVEKIPEIPL 150
    VVSTDLESIQ KTKEAVAALK AVGAHSDLLK VLKSKKLRAG KGKYRNRRWT 200
    QRRGPLVVYA EDNGIVKALR NVPGVETANV ASLNLLQLAP GAHLGRFVIW 250
    TEAAFTKLDQ VWGSETVASS KVGYTLPSHI ISTSDVTRII NSSEIQSAIR 300
    PAGQATQKRT HVLKKNPLKN KQVLLRLNPY AKVFAAEKLG SKKAEKTGTK 350
    PAAVFTETLK HD 362
    Length:362
    Mass (Da):39,092
    Last modified:January 23, 2007 - v4
    Checksum:i37D0942A10AAE89F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti38 – 381V → L in AAA34974. (PubMed:2834365)Curated
    Sequence conflicti144 – 1441K → T in AAA34974. (PubMed:2834365)Curated
    Sequence conflicti157 – 1571E → D in AAA34974. (PubMed:2834365)Curated
    Sequence conflicti224 – 2241G → S in AAA34974. (PubMed:2834365)Curated
    Sequence conflicti241 – 2411G → S in AAA34974. (PubMed:2834365)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76078 Genomic DNA. Translation: CAA53687.1.
    J03195 Genomic DNA. Translation: AAA34974.1.
    Z35900 Genomic DNA. Translation: CAA84973.1.
    BK006936 Genomic DNA. Translation: DAA07152.1.
    PIRiS45887.
    RefSeqiNP_009587.1. NM_001178379.1.

    Genome annotation databases

    EnsemblFungiiYBR031W; YBR031W; YBR031W.
    GeneIDi852319.
    KEGGisce:YBR031W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76078 Genomic DNA. Translation: CAA53687.1 .
    J03195 Genomic DNA. Translation: AAA34974.1 .
    Z35900 Genomic DNA. Translation: CAA84973.1 .
    BK006936 Genomic DNA. Translation: DAA07152.1 .
    PIRi S45887.
    RefSeqi NP_009587.1. NM_001178379.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K5Y model - D 5-260 [» ]
    1VW8 electron microscopy 6.10 D 1-362 [» ]
    1VWU electron microscopy 6.10 D 1-362 [» ]
    3IZS electron microscopy - D 1-362 [» ]
    3J65 electron microscopy 8.70 D 1-362 [» ]
    3O58 X-ray 4.00 D 1-362 [» ]
    3O5H X-ray 4.00 D 1-362 [» ]
    3U5E X-ray 3.00 C 1-362 [» ]
    3U5I X-ray 3.00 C 1-362 [» ]
    4B6A electron microscopy 8.10 C 1-362 [» ]
    4BYN electron microscopy 4.30 C 2-362 [» ]
    4BYU electron microscopy 6.60 C 2-362 [» ]
    4CUW electron microscopy 3.70 C 1-362 [» ]
    ProteinModelPortali P10664.
    SMRi P10664. Positions 2-362.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32733. 190 interactions.
    IntActi P10664. 217 interactions.
    MINTi MINT-1325639.
    STRINGi 4932.YBR031W.

    Proteomic databases

    MaxQBi P10664.
    PeptideAtlasi P10664.
    PRIDEi P10664.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR031W ; YBR031W ; YBR031W .
    GeneIDi 852319.
    KEGGi sce:YBR031W.

    Organism-specific databases

    SGDi S000000235. RPL4A.

    Phylogenomic databases

    GeneTreei ENSGT00390000018145.
    HOGENOMi HOG000107331.
    KOi K02930.
    OMAi VASNENK.
    OrthoDBi EOG7NSBC8.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29009-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    NextBioi 971014.

    Gene expression databases

    Genevestigatori P10664.

    Family and domain databases

    Gene3Di 3.40.1370.10. 1 hit.
    InterProi IPR025755. Ribos_L4_C_dom.
    IPR002136. Ribosomal_L4/L1e.
    IPR013000. Ribosomal_L4/L1e_euk/arc_CS.
    IPR023574. Ribosomal_L4_dom.
    [Graphical view ]
    Pfami PF14374. Ribos_L4_asso_C. 1 hit.
    PF00573. Ribosomal_L4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52166. SSF52166. 1 hit.
    PROSITEi PS00939. RIBOSOMAL_L1E. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Ribosomal protein L2 in Saccharomyces cerevisiae is homologous to ribosomal protein L1 in Xenopus laevis. Isolation and characterization of the genes."
      Presutti C., Lucioli A., Bozzoni I.
      J. Biol. Chem. 263:6188-6192(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene."
      Smits P.H.M., de Haan M., Maat C., Grivell L.A.
      Yeast 10:S75-S80(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The ribosomal protein L2 in S. cerevisiae controls the level of accumulation of its own mRNA."
      Presutti C., Ciafre S.-A., Bozzoni I.
      EMBO J. 10:2215-2221(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN L2 MRNA REGULATION.
    6. "NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
      Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
      J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-21, ACETYLATION AT SER-2 BY NATA.
    7. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
      Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
      Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 5-260, ELECTRON MICROSCOPY.
    10. "Crystal structure of the eukaryotic ribosome."
      Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
      Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
    11. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

    Entry informationi

    Entry nameiRL4A_YEAST
    AccessioniPrimary (citable) accession number: P10664
    Secondary accession number(s): D6VQ32
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 130 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are 2 genes for L4 in yeast.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3