Reviewed, UniProtKB/Swiss-Prot P10659 (METK1_YEAST)

Names and origin

Protein names

Recommended name:
    S-adenosylmethionine synthetase 1
      Short name=AdoMet synthetase 1
    EC=2.5.1.6
Alternative name(s):
    Methionine adenosyltransferase 1
      Short name=MAT 1

Gene names

Name:	SAM1
Synonyms:	ETH10
Ordered Locus Names:	YLR180W
ORF Names:	L9470.9

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Subunit structure	Heterotetramer.
Miscellaneous	In yeast, there are two genes coding for AdoMet synthetase. Present with 103000 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the AdoMet synthetase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	S-adenosylmethionine biosynthetic process Inferred from mutant phenotype. Source: SGD methionine metabolic process Inferred from mutant phenotype. Source: SGD one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activity Ref.1 Inferred from genetic interaction. Source: SGD potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	S-adenosylmethionine synthetase 1		PRO_0000174451
Regions
	Nucleotide binding	118 – 123	6	ATP Potential
Sites
	Metal binding	18	1	Magnesium By similarity
	Metal binding	44	1	Potassium By similarity
	Metal binding	270	1	Potassium By similarity
	Metal binding	278	1	Magnesium By similarity
	Binding site	146	1	ATP Potential
Experimental info
	Sequence conflict	246	1	A → T in AAA66932. Ref.1
	Sequence conflict	357	1	I → F in AAA66932. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P10659-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 18F82FC809EE4706	FASTA	382	41,818
	10 20 30 40 50 60 MAGTFLFTSE SVGEGHPDKI CDQVSDAILD ACLAEDPHSK VACETAAKTG MIMVFGEITT 70 80 90 100 110 120 KAQLDYQKIV RDTIKKIGYD DSAKGFDYKT CNVLVAIEQQ SPDIAQGVHE EKDLEDIGAG 130 140 150 160 170 180 DQGIMFGYAT DETPEGLPLT ILLAHKLNMA MADARRDGSL AWLRPDTKTQ VTVEYKDDHG 190 200 210 220 230 240 RWVPQRIDTV VVSAQHADEI TTEDLRAQLK SEIIEKVIPR DMLDENTKYF IQPSGRFVIG 250 260 270 280 290 300 GPQGDAGLTG RKIIVDAYGG ASSVGGGAFS GKDYSKVDRS AAYAARWVAK SLVAAGLCKR 310 320 330 340 350 360 VQVQFSYAIG IAEPLSLHVD TYGTATKSDE EIIDIISKNF DLRPGVLVKE LDLARPIYLP 370 380 TASYGHFTNQ EYPWEKPKTL KF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SAM1, the structural gene for one of the S-adenosylmethionine synthetases in Saccharomyces cerevisiae. Sequence and expression." Thomas D., Surdin-Kerjan Y. J. Biol. Chem. 262:16704-16709(1987) [PubMed: 3316224] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae." Norbeck J., Blomberg A. FEMS Microbiol. Lett. 137:1-8(1996) [PubMed: 8935650] [Abstract] Cited for: PROTEIN SEQUENCE OF 157-162; 187-196 AND 253-258. Strain: ATCC 38531 / Y41.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J03477 Genomic DNA. Translation: AAA66932.1. U17246 Genomic DNA. Translation: AAB67461.1.
PIR	S51425.
RefSeq	NP_013281.1.
3D structure databases
HSSP	HSSP built from PDB template 1QM4 based on UniProtKB P13444.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6667N.
IntAct	P10659. 86 interactions.
STRING	P10659.
Proteomic databases
PeptideAtlas	P10659.
PRIDE	P10659.
Genome annotation databases
Ensembl	YLR180W; YLR180W; YLR180W; Saccharomyces cerevisiae. [Genome view]
GeneID	850877.
GenomeReviews	Gene locus YLR180W in contig Y13138_GR.
KEGG	sce:YLR180W.
NMPDR	fig|4932.3.peg.4291.
Organism-specific databases
CYGD	YLR180w.
SGD	S000004170. SAM1.
Phylogenomic databases
HOGENOM	P10659.
OMA	LHYEKAL.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-404.
BRENDA	2.5.1.6. 250.
Gene expression databases
ArrayExpress	P10659.
Genevestigator	P10659.
GermOnline	YLR180W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002133. S-AdoMet_synthetase. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
TIGRFAMs	TIGR01034. metK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHETASE_1. 1 hit. PS00377. ADOMET_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	967224.

Entry information

Entry name

METK1_YEAST

Accession

Primary (citable) accession number: P10659

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	February 1, 1996
Last modified:	November 3, 2009
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)