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Protein

S-adenosylmethionine synthase 1

Gene

SAM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.1 Publication

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.By similarity
  • K+By similarityNote: Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.By similarity

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase 1 (SAM1), S-adenosylmethionine synthase 2 (SAM2)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10MagnesiumBy similarity1
Binding sitei16ATPBy similarity1
Metal bindingi44PotassiumBy similarity1
Binding sitei57MethionineBy similarity1
Binding sitei100MethionineBy similarity1
Binding sitei245ATPBy similarity1
Binding sitei245Methionine; shared with neighboring subunitBy similarity1
Binding sitei268ATP; via amide nitrogen; shared with neighboring subunitBy similarity1
Binding sitei272ATP; shared with neighboring subunitBy similarity1
Binding sitei276ATP; shared with neighboring subunitBy similarity1
Binding sitei276MethionineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi166 – 168ATPBy similarity3
Nucleotide bindingi234 – 237ATPBy similarity4
Nucleotide bindingi251 – 252ATPBy similarity2

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • methionine adenosyltransferase activity Source: SGD

GO - Biological processi

  • methionine metabolic process Source: SGD
  • one-carbon metabolic process Source: UniProtKB-KW
  • S-adenosylmethionine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciMetaCyc:YLR180W-MONOMER.
YEAST:YLR180W-MONOMER.
ReactomeiR-SCE-156581. Methylation.
R-SCE-1614635. Sulfur amino acid metabolism.
R-SCE-2408508. Metabolism of ingested SeMet, Sec, MeSec into H2Se.
UniPathwayiUPA00315; UER00080.

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthase 1 (EC:2.5.1.61 Publication)
Short name:
AdoMet synthase 1
Alternative name(s):
Methionine adenosyltransferase 1
Short name:
MAT 1
Gene namesi
Name:SAM1
Synonyms:ETH10
Ordered Locus Names:YLR180W
ORF Names:L9470.9
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR180W.
SGDiS000004170. SAM1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic stress granule Source: SGD
  • cytosol Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001744511 – 382S-adenosylmethionine synthase 1Add BLAST382

Proteomic databases

MaxQBiP10659.
PRIDEiP10659.

Interactioni

Subunit structurei

Heterotetramer.

Protein-protein interaction databases

BioGridi31451. 129 interactors.
DIPiDIP-6667N.
IntActiP10659. 92 interactors.
MINTiMINT-626539.

Structurei

3D structure databases

ProteinModelPortaliP10659.
SMRiP10659.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AdoMet synthase family.Curated

Phylogenomic databases

GeneTreeiENSGT00500000044811.
HOGENOMiHOG000245710.
InParanoidiP10659.
KOiK00789.
OMAiACEVMAC.
OrthoDBiEOG092C2SCC.

Family and domain databases

HAMAPiMF_00086. S_AdoMet_synth1. 1 hit.
InterProiIPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
[Graphical view]
PANTHERiPTHR11964. PTHR11964. 1 hit.
PfamiPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000497. MAT. 1 hit.
SUPFAMiSSF55973. SSF55973. 3 hits.
TIGRFAMsiTIGR01034. metK. 1 hit.
PROSITEiPS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10659-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGTFLFTSE SVGEGHPDKI CDQVSDAILD ACLAEDPHSK VACETAAKTG
60 70 80 90 100
MIMVFGEITT KAQLDYQKIV RDTIKKIGYD DSAKGFDYKT CNVLVAIEQQ
110 120 130 140 150
SPDIAQGVHE EKDLEDIGAG DQGIMFGYAT DETPEGLPLT ILLAHKLNMA
160 170 180 190 200
MADARRDGSL AWLRPDTKTQ VTVEYKDDHG RWVPQRIDTV VVSAQHADEI
210 220 230 240 250
TTEDLRAQLK SEIIEKVIPR DMLDENTKYF IQPSGRFVIG GPQGDAGLTG
260 270 280 290 300
RKIIVDAYGG ASSVGGGAFS GKDYSKVDRS AAYAARWVAK SLVAAGLCKR
310 320 330 340 350
VQVQFSYAIG IAEPLSLHVD TYGTATKSDE EIIDIISKNF DLRPGVLVKE
360 370 380
LDLARPIYLP TASYGHFTNQ EYPWEKPKTL KF
Length:382
Mass (Da):41,818
Last modified:February 1, 1996 - v2
Checksum:i18F82FC809EE4706
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti246A → T in AAA66932 (PubMed:3316224).Curated1
Sequence conflicti357I → F in AAA66932 (PubMed:3316224).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03477 Genomic DNA. Translation: AAA66932.1.
U17246 Genomic DNA. Translation: AAB67461.1.
BK006945 Genomic DNA. Translation: DAA09500.1.
PIRiS51425.
RefSeqiNP_013281.1. NM_001182067.1.

Genome annotation databases

EnsemblFungiiYLR180W; YLR180W; YLR180W.
GeneIDi850877.
KEGGisce:YLR180W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03477 Genomic DNA. Translation: AAA66932.1.
U17246 Genomic DNA. Translation: AAB67461.1.
BK006945 Genomic DNA. Translation: DAA09500.1.
PIRiS51425.
RefSeqiNP_013281.1. NM_001182067.1.

3D structure databases

ProteinModelPortaliP10659.
SMRiP10659.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31451. 129 interactors.
DIPiDIP-6667N.
IntActiP10659. 92 interactors.
MINTiMINT-626539.

Proteomic databases

MaxQBiP10659.
PRIDEiP10659.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR180W; YLR180W; YLR180W.
GeneIDi850877.
KEGGisce:YLR180W.

Organism-specific databases

EuPathDBiFungiDB:YLR180W.
SGDiS000004170. SAM1.

Phylogenomic databases

GeneTreeiENSGT00500000044811.
HOGENOMiHOG000245710.
InParanoidiP10659.
KOiK00789.
OMAiACEVMAC.
OrthoDBiEOG092C2SCC.

Enzyme and pathway databases

UniPathwayiUPA00315; UER00080.
BioCyciMetaCyc:YLR180W-MONOMER.
YEAST:YLR180W-MONOMER.
ReactomeiR-SCE-156581. Methylation.
R-SCE-1614635. Sulfur amino acid metabolism.
R-SCE-2408508. Metabolism of ingested SeMet, Sec, MeSec into H2Se.

Miscellaneous databases

PROiP10659.

Family and domain databases

HAMAPiMF_00086. S_AdoMet_synth1. 1 hit.
InterProiIPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
[Graphical view]
PANTHERiPTHR11964. PTHR11964. 1 hit.
PfamiPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000497. MAT. 1 hit.
SUPFAMiSSF55973. SSF55973. 3 hits.
TIGRFAMsiTIGR01034. metK. 1 hit.
PROSITEiPS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMETK1_YEAST
AccessioniPrimary (citable) accession number: P10659
Secondary accession number(s): D6VYI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 168 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In yeast, there are two genes coding for AdoMet synthase.
Present with 103000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.