Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P10656 (GLNA_CLOSA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase

EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase
Gene names
Name:glnA
OrganismClostridium saccharobutylicum
Taxonomic identifier169679 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length443 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Oligomer of 12 subunits arranged in the form of two hexagons.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the glutamine synthetase family.

Caution

Was originally thought to originate from C.acetobutylicum.

Lacks the tyrosine conserved in bacteria and other archaea, involved in feedback inhibition.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 443443Glutamine synthetase
PRO_0000153234

Sequences

Sequence LengthMass (Da)Tools
P10656 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: EDE5BF44495151FC

FASTA44349,682
        10         20         30         40         50         60 
MAKYTKEDII NLVKENGVKF IRLQFTDIFG TLKNVAITDK QLEKALDNEC MFDGSSIDGF 

        70         80         90        100        110        120 
VRIEESDMNL RPNLDSFVIF PWRPQQGKVA RLICDVYKPD GTPFEGDPRH VLKRANADAK 

       130        140        150        160        170        180 
ELGYTMNVGP ECEFFLFETD ENGRATTNTQ DKAGYFDLAP TDLGENARRD MTLALEEMGF 

       190        200        210        220        230        240 
EIEASHHEVA EGQNEIDFKY GDALTTADNI MTFKLVVKSI AQRHGLHASF MPKPIFGING 

       250        260        270        280        290        300 
SGMHVNMSLF KDGKNAFVDE NDKNGLSKVA YQFIAGLLKN IKGMAAVTNP LVNSYKRLVP 

       310        320        330        340        350        360 
GYEAPVYLAW SCKNRTALIR VPAARGAGTR VELRCPDPSS NPYLVLACLL QAGLDGIKNN 

       370        380        390        400        410        420 
LQPPAEVEAN IFAMTEQERK ENGIDNLPNN LYEAVNYMKE NELAKKALGD HVYGNYVAGK 

       430        440 
AAEWDDYRTK VHDWELENYL NKY 

« Hide

References

[1]"Molecular analysis and regulation of the glnA gene of the Gram-positive anaerobe Clostridium acetobutylicum."
Janssen P.J., Jones W.A., Jones D.T., Woods D.R.
J. Bacteriol. 170:400-408(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC BAA-117 / DSM 13864 / NCP 262.
[2]"Studies on Clostridium acetobutylicum glnA promoters and antisense RNA."
Janssen P.J., Jones D.T., Woods D.R.
Mol. Microbiol. 4:1575-1583(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-38.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M18966 Genomic DNA. Translation: AAA23241.1.
PIRAJCLQA. A28676.

3D structure databases

ProteinModelPortalP10656.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
TIGRFAMsTIGR00653. GlnA. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA_CLOSA
AccessionPrimary (citable) accession number: P10656
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 16, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families