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Protein

Glutamine synthetase

Gene

glnA

Organism
Clostridium saccharobutylicum
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase
Gene namesi
Name:glnA
OrganismiClostridium saccharobutylicum
Taxonomic identifieri169679 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 443443Glutamine synthetasePRO_0000153234Add
BLAST

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.

Structurei

3D structure databases

ProteinModelPortaliP10656.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10656-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKYTKEDII NLVKENGVKF IRLQFTDIFG TLKNVAITDK QLEKALDNEC
60 70 80 90 100
MFDGSSIDGF VRIEESDMNL RPNLDSFVIF PWRPQQGKVA RLICDVYKPD
110 120 130 140 150
GTPFEGDPRH VLKRANADAK ELGYTMNVGP ECEFFLFETD ENGRATTNTQ
160 170 180 190 200
DKAGYFDLAP TDLGENARRD MTLALEEMGF EIEASHHEVA EGQNEIDFKY
210 220 230 240 250
GDALTTADNI MTFKLVVKSI AQRHGLHASF MPKPIFGING SGMHVNMSLF
260 270 280 290 300
KDGKNAFVDE NDKNGLSKVA YQFIAGLLKN IKGMAAVTNP LVNSYKRLVP
310 320 330 340 350
GYEAPVYLAW SCKNRTALIR VPAARGAGTR VELRCPDPSS NPYLVLACLL
360 370 380 390 400
QAGLDGIKNN LQPPAEVEAN IFAMTEQERK ENGIDNLPNN LYEAVNYMKE
410 420 430 440
NELAKKALGD HVYGNYVAGK AAEWDDYRTK VHDWELENYL NKY
Length:443
Mass (Da):49,682
Last modified:July 1, 1989 - v1
Checksum:iEDE5BF44495151FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18966 Genomic DNA. Translation: AAA23241.1.
PIRiA28676. AJCLQA.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18966 Genomic DNA. Translation: AAA23241.1.
PIRiA28676. AJCLQA.

3D structure databases

ProteinModelPortaliP10656.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular analysis and regulation of the glnA gene of the Gram-positive anaerobe Clostridium acetobutylicum."
    Janssen P.J., Jones W.A., Jones D.T., Woods D.R.
    J. Bacteriol. 170:400-408(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC BAA-117 / DSM 13864 / NCP 262.
  2. "Studies on Clostridium acetobutylicum glnA promoters and antisense RNA."
    Janssen P.J., Jones D.T., Woods D.R.
    Mol. Microbiol. 4:1575-1583(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-38.

Entry informationi

Entry nameiGLNA_CLOSA
AccessioniPrimary (citable) accession number: P10656
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: January 7, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to originate from C.acetobutylicum.Curated
Lacks the tyrosine conserved in bacteria and other archaea, involved in feedback inhibition.Curated

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.