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Protein

Glutathione S-transferase Mu 1

Gene

Gstm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50GlutathioneBy similarity1
Binding sitei116SubstrateBy similarity1

GO - Molecular functioni

  • glutathione transferase activity Source: MGI
  • protein heterodimerization activity Source: MGI
  • protein homodimerization activity Source: MGI

GO - Biological processi

  • cellular response to drug Source: MGI
  • glutathione metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase Mu 1 (EC:2.5.1.18)
Alternative name(s):
GST 1-1
GST class-mu 1
Glutathione S-transferase GT8.7
pmGT10
Gene namesi
Name:Gstm1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:95860. Gstm1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • myelin sheath Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3722.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001858262 – 218Glutathione S-transferase Mu 1Add BLAST217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphothreonineCombined sources1
Modified residuei67PhosphoserineBy similarity1
Modified residuei210PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP10649.
MaxQBiP10649.
PaxDbiP10649.
PeptideAtlasiP10649.
PRIDEiP10649.

2D gel databases

REPRODUCTION-2DPAGEP10649.
SWISS-2DPAGEP10649.

PTM databases

iPTMnetiP10649.
PhosphoSitePlusiP10649.
SwissPalmiP10649.

Expressioni

Gene expression databases

BgeeiENSMUSG00000058135.
CleanExiMM_GSTM1.
ExpressionAtlasiP10649. baseline and differential.
GenevisibleiP10649. MM.

Interactioni

Subunit structurei

Homodimer.

GO - Molecular functioni

  • protein heterodimerization activity Source: MGI
  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

BioGridi200094. 3 interactors.
IntActiP10649. 6 interactors.
MINTiMINT-1869211.
STRINGi10090.ENSMUSP00000004140.

Chemistry databases

BindingDBiP10649.

Structurei

3D structure databases

ProteinModelPortaliP10649.
SMRiP10649.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 88GST N-terminalAdd BLAST87
Domaini90 – 208GST C-terminalAdd BLAST119

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni7 – 8Glutathione bindingBy similarity2
Regioni43 – 46Glutathione bindingBy similarity4
Regioni59 – 60Glutathione bindingBy similarity2
Regioni72 – 73Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Mu family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiENOG410IN5J. Eukaryota.
ENOG4110YU0. LUCA.
GeneTreeiENSGT00550000074559.
HOGENOMiHOG000115735.
HOVERGENiHBG106842.
InParanoidiP10649.
KOiK00799.
PhylomeDBiP10649.
TreeFamiTF353040.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR003081. GST_mu.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01267. GSTRNSFRASEM.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10649-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK
60 70 80 90 100
FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV
110 120 130 140 150
ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG
160 170 180 190 200
DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS
210
SRYIATPIFS KMAHWSNK
Length:218
Mass (Da):25,970
Last modified:January 23, 2007 - v2
Checksum:i91AE34A1B5B44599
GO

Mass spectrometryi

Molecular mass is 25838.4±2 Da from positions 2 - 218. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03952 mRNA. Translation: AAA37747.1.
J04632 mRNA. Translation: AAA37705.1.
L13448 Genomic DNA. No translation available.
AL671877, AC079042 Genomic DNA. Translation: CAM17697.1.
BC003822 mRNA. Translation: AAH03822.1.
BC046758 mRNA. Translation: AAH46758.1.
BC091763 mRNA. Translation: AAH91763.1.
CCDSiCCDS17747.1.
PIRiH24735.
S30373.
S33860.
RefSeqiNP_034488.1. NM_010358.5.
UniGeneiMm.37199.

Genome annotation databases

EnsembliENSMUST00000004140; ENSMUSP00000004140; ENSMUSG00000058135.
GeneIDi14862.
KEGGimmu:14862.
UCSCiuc008qxx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03952 mRNA. Translation: AAA37747.1.
J04632 mRNA. Translation: AAA37705.1.
L13448 Genomic DNA. No translation available.
AL671877, AC079042 Genomic DNA. Translation: CAM17697.1.
BC003822 mRNA. Translation: AAH03822.1.
BC046758 mRNA. Translation: AAH46758.1.
BC091763 mRNA. Translation: AAH91763.1.
CCDSiCCDS17747.1.
PIRiH24735.
S30373.
S33860.
RefSeqiNP_034488.1. NM_010358.5.
UniGeneiMm.37199.

3D structure databases

ProteinModelPortaliP10649.
SMRiP10649.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200094. 3 interactors.
IntActiP10649. 6 interactors.
MINTiMINT-1869211.
STRINGi10090.ENSMUSP00000004140.

Chemistry databases

BindingDBiP10649.
ChEMBLiCHEMBL3722.

PTM databases

iPTMnetiP10649.
PhosphoSitePlusiP10649.
SwissPalmiP10649.

2D gel databases

REPRODUCTION-2DPAGEP10649.
SWISS-2DPAGEP10649.

Proteomic databases

EPDiP10649.
MaxQBiP10649.
PaxDbiP10649.
PeptideAtlasiP10649.
PRIDEiP10649.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004140; ENSMUSP00000004140; ENSMUSG00000058135.
GeneIDi14862.
KEGGimmu:14862.
UCSCiuc008qxx.2. mouse.

Organism-specific databases

CTDi2944.
MGIiMGI:95860. Gstm1.

Phylogenomic databases

eggNOGiENOG410IN5J. Eukaryota.
ENOG4110YU0. LUCA.
GeneTreeiENSGT00550000074559.
HOGENOMiHOG000115735.
HOVERGENiHBG106842.
InParanoidiP10649.
KOiK00799.
PhylomeDBiP10649.
TreeFamiTF353040.

Miscellaneous databases

ChiTaRSiGstm1. mouse.
PROiP10649.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000058135.
CleanExiMM_GSTM1.
ExpressionAtlasiP10649. baseline and differential.
GenevisibleiP10649. MM.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR003081. GST_mu.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01267. GSTRNSFRASEM.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTM1_MOUSE
AccessioniPrimary (citable) accession number: P10649
Secondary accession number(s): A2AE90, Q58ET5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 167 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.