Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutathione S-transferase A2

Gene

Gsta2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91GlutathioneBy similarity
Binding sitei45 – 451Glutathione1 Publication
Binding sitei55 – 551Glutathione; via amide nitrogen and carbonyl oxygen1 Publication

GO - Molecular functioni

GO - Biological processi

  • glutathione metabolic process Source: UniProtKB
  • response to stilbenoid Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiREACT_317599. Glutathione conjugation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase A2 (EC:2.5.1.18)
Alternative name(s):
GST class-alpha member 2
Glutathione S-transferase GT41A
Gene namesi
Name:Gsta2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:95863. Gsta2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 222221Glutathione S-transferase A2PRO_0000185789Add
BLAST

Proteomic databases

PaxDbiP10648.

PTM databases

PhosphoSiteiP10648.

Expressioni

Gene expression databases

CleanExiMM_GSTA2.
ExpressionAtlasiP10648. baseline and differential.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiP10648. 2 interactions.
MINTiMINT-4080211.
STRINGi10090.ENSMUSP00000034902.

Structurei

Secondary structure

1
222
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 127Combined sources
Helixi14 – 163Combined sources
Helixi17 – 2610Combined sources
Beta strandi31 – 355Combined sources
Helixi38 – 469Combined sources
Beta strandi57 – 604Combined sources
Beta strandi63 – 675Combined sources
Helixi68 – 7811Combined sources
Helixi86 – 10823Combined sources
Helixi109 – 1113Combined sources
Turni114 – 1163Combined sources
Helixi117 – 13014Combined sources
Helixi132 – 14312Combined sources
Beta strandi146 – 1494Combined sources
Helixi155 – 17117Combined sources
Helixi172 – 1754Combined sources
Helixi179 – 19012Combined sources
Helixi192 – 1987Combined sources
Helixi210 – 21910Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ML6X-ray1.90A/B2-222[»]
ProteinModelPortaliP10648.
SMRiP10648. Positions 3-222.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10648.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 8381GST N-terminalAdd
BLAST
Domaini85 – 208124GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 682Glutathione binding

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiNOG266414.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiP10648.
KOiK00799.
OMAiKERAXID.
OrthoDBiEOG79CZ0K.
TreeFamiTF105321.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10648-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKPVLHYF NARGRMECIR WLLAAAGVEF EEKFIQSPED LEKLKKDGNL
60 70 80 90 100
MFDQVPMVEI DGMKLVQTRA ILNYIATKYD LYGKDMKERA LIDMYTEGIL
110 120 130 140 150
DLTEMIGQLV LCPPDQREAK TALAKDRTKN RYLPAFEKVL KSHGQDYLVG
160 170 180 190 200
NRLTRVDVHL LELLLYVEEL DASLLTPFPL LKAFKSRISS LPNVKKFLHP
210 220
GSQRKPPLDA KQIEEARKVF KF
Length:222
Mass (Da):25,542
Last modified:October 3, 2012 - v3
Checksum:i837FFFC49C56F70F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti199 – 1991H → Q in AAA37749 (PubMed:3417659).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03958 mRNA. Translation: AAA37749.1.
AC138587 Genomic DNA. No translation available.
CH466522 Genomic DNA. Translation: EDL26384.1.
CH466522 Genomic DNA. Translation: EDL26385.1.
CH466522 Genomic DNA. Translation: EDL26386.1.
CH466522 Genomic DNA. Translation: EDL26387.1.
BC061133 mRNA. Translation: AAH61133.1.
CCDSiCCDS23359.1.
RefSeqiNP_032208.2. NM_008182.3.
XP_006510877.1. XM_006510814.2.
XP_011240965.1. XM_011242663.1.
UniGeneiMm.422778.

Genome annotation databases

EnsembliENSMUST00000034902; ENSMUSP00000034902; ENSMUSG00000057933.
GeneIDi14858.
KEGGimmu:14858.
UCSCiuc009quf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03958 mRNA. Translation: AAA37749.1.
AC138587 Genomic DNA. No translation available.
CH466522 Genomic DNA. Translation: EDL26384.1.
CH466522 Genomic DNA. Translation: EDL26385.1.
CH466522 Genomic DNA. Translation: EDL26386.1.
CH466522 Genomic DNA. Translation: EDL26387.1.
BC061133 mRNA. Translation: AAH61133.1.
CCDSiCCDS23359.1.
RefSeqiNP_032208.2. NM_008182.3.
XP_006510877.1. XM_006510814.2.
XP_011240965.1. XM_011242663.1.
UniGeneiMm.422778.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ML6X-ray1.90A/B2-222[»]
ProteinModelPortaliP10648.
SMRiP10648. Positions 3-222.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP10648. 2 interactions.
MINTiMINT-4080211.
STRINGi10090.ENSMUSP00000034902.

PTM databases

PhosphoSiteiP10648.

Proteomic databases

PaxDbiP10648.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034902; ENSMUSP00000034902; ENSMUSG00000057933.
GeneIDi14858.
KEGGimmu:14858.
UCSCiuc009quf.1. mouse.

Organism-specific databases

CTDi2939.
MGIiMGI:95863. Gsta2.

Phylogenomic databases

eggNOGiNOG266414.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiP10648.
KOiK00799.
OMAiKERAXID.
OrthoDBiEOG79CZ0K.
TreeFamiTF105321.

Enzyme and pathway databases

ReactomeiREACT_317599. Glutathione conjugation.

Miscellaneous databases

EvolutionaryTraceiP10648.
NextBioi287091.
PROiP10648.
SOURCEiSearch...

Gene expression databases

CleanExiMM_GSTA2.
ExpressionAtlasiP10648. baseline and differential.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Tissue-specific induction of murine glutathione transferase mRNAs by butylated hydroxyanisole."
    Pearson W.R., Reinhart J., Sisk S.C., Anderson K.S., Adler P.N.
    J. Biol. Chem. 263:13324-13332(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  5. "Residues 207, 216, and 221 and the catalytic activity of mGSTA1-1 and mGSTA2-2 toward benzo[a]pyrene-(7R,8S)-diol-(9S,10R)-epoxide."
    Gu Y., Xiao B., Wargo H.L., Bucher M.H., Singh S.V., Ji X.
    Biochemistry 42:917-921(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH GLUTATHIONE AND SUBSTRATE ANALOG, SUBUNIT.

Entry informationi

Entry nameiGSTA2_MOUSE
AccessioniPrimary (citable) accession number: P10648
Secondary accession number(s): Q6P8Q1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 3, 2012
Last modified: July 22, 2015
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.