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P10648

- GSTA2_MOUSE

UniProt

P10648 - GSTA2_MOUSE

Protein

Glutathione S-transferase A2

Gene

Gsta2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 3 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei9 – 91GlutathioneBy similarity
    Binding sitei45 – 451Glutathione1 Publication
    Binding sitei55 – 551Glutathione; via amide nitrogen and carbonyl oxygen1 Publication

    GO - Molecular functioni

    1. glutathione transferase activity Source: UniProtKB

    GO - Biological processi

    1. glutathione metabolic process Source: UniProtKB
    2. response to stilbenoid Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    ReactomeiREACT_215316. Glutathione conjugation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase A2 (EC:2.5.1.18)
    Alternative name(s):
    GST class-alpha member 2
    Glutathione S-transferase GT41A
    Gene namesi
    Name:Gsta2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:95863. Gsta2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 222221Glutathione S-transferase A2PRO_0000185789Add
    BLAST

    Proteomic databases

    PaxDbiP10648.

    PTM databases

    PhosphoSiteiP10648.

    Expressioni

    Gene expression databases

    CleanExiMM_GSTA2.
    GenevestigatoriP10648.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    IntActiP10648. 2 interactions.
    MINTiMINT-4080211.
    STRINGi10090.ENSMUSP00000034902.

    Structurei

    Secondary structure

    1
    222
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 127
    Helixi14 – 163
    Helixi17 – 2610
    Beta strandi31 – 355
    Helixi38 – 469
    Beta strandi57 – 604
    Beta strandi63 – 675
    Helixi68 – 7811
    Helixi86 – 10823
    Helixi109 – 1113
    Turni114 – 1163
    Helixi117 – 13014
    Helixi132 – 14312
    Beta strandi146 – 1494
    Helixi155 – 17117
    Helixi172 – 1754
    Helixi179 – 19012
    Helixi192 – 1987
    Helixi210 – 21910

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ML6X-ray1.90A/B2-222[»]
    ProteinModelPortaliP10648.
    SMRiP10648. Positions 3-222.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP10648.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 8381GST N-terminalAdd
    BLAST
    Domaini85 – 208124GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni67 – 682Glutathione binding

    Sequence similaritiesi

    Belongs to the GST superfamily. Alpha family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiNOG266414.
    GeneTreeiENSGT00670000097856.
    HOGENOMiHOG000115734.
    HOVERGENiHBG053749.
    InParanoidiP10648.
    KOiK00799.
    OMAiICQPEER.
    OrthoDBiEOG79CZ0K.
    TreeFamiTF105321.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR003080. GST_alpha.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view]
    PRINTSiPR01266. GSTRNSFRASEA.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10648-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGKPVLHYF NARGRMECIR WLLAAAGVEF EEKFIQSPED LEKLKKDGNL    50
    MFDQVPMVEI DGMKLVQTRA ILNYIATKYD LYGKDMKERA LIDMYTEGIL 100
    DLTEMIGQLV LCPPDQREAK TALAKDRTKN RYLPAFEKVL KSHGQDYLVG 150
    NRLTRVDVHL LELLLYVEEL DASLLTPFPL LKAFKSRISS LPNVKKFLHP 200
    GSQRKPPLDA KQIEEARKVF KF 222
    Length:222
    Mass (Da):25,542
    Last modified:October 3, 2012 - v3
    Checksum:i837FFFC49C56F70F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti199 – 1991H → Q in AAA37749. (PubMed:3417659)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03958 mRNA. Translation: AAA37749.1.
    AC138587 Genomic DNA. No translation available.
    CH466522 Genomic DNA. Translation: EDL26384.1.
    CH466522 Genomic DNA. Translation: EDL26385.1.
    CH466522 Genomic DNA. Translation: EDL26386.1.
    CH466522 Genomic DNA. Translation: EDL26387.1.
    BC061133 mRNA. Translation: AAH61133.1.
    CCDSiCCDS23359.1.
    RefSeqiNP_032208.2. NM_008182.3.
    XP_006510877.1. XM_006510814.1.
    UniGeneiMm.422778.

    Genome annotation databases

    EnsembliENSMUST00000034902; ENSMUSP00000034902; ENSMUSG00000057933.
    GeneIDi14858.
    KEGGimmu:14858.
    UCSCiuc009quf.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03958 mRNA. Translation: AAA37749.1 .
    AC138587 Genomic DNA. No translation available.
    CH466522 Genomic DNA. Translation: EDL26384.1 .
    CH466522 Genomic DNA. Translation: EDL26385.1 .
    CH466522 Genomic DNA. Translation: EDL26386.1 .
    CH466522 Genomic DNA. Translation: EDL26387.1 .
    BC061133 mRNA. Translation: AAH61133.1 .
    CCDSi CCDS23359.1.
    RefSeqi NP_032208.2. NM_008182.3.
    XP_006510877.1. XM_006510814.1.
    UniGenei Mm.422778.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ML6 X-ray 1.90 A/B 2-222 [» ]
    ProteinModelPortali P10648.
    SMRi P10648. Positions 3-222.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P10648. 2 interactions.
    MINTi MINT-4080211.
    STRINGi 10090.ENSMUSP00000034902.

    PTM databases

    PhosphoSitei P10648.

    Proteomic databases

    PaxDbi P10648.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034902 ; ENSMUSP00000034902 ; ENSMUSG00000057933 .
    GeneIDi 14858.
    KEGGi mmu:14858.
    UCSCi uc009quf.1. mouse.

    Organism-specific databases

    CTDi 2939.
    MGIi MGI:95863. Gsta2.

    Phylogenomic databases

    eggNOGi NOG266414.
    GeneTreei ENSGT00670000097856.
    HOGENOMi HOG000115734.
    HOVERGENi HBG053749.
    InParanoidi P10648.
    KOi K00799.
    OMAi ICQPEER.
    OrthoDBi EOG79CZ0K.
    TreeFami TF105321.

    Enzyme and pathway databases

    Reactomei REACT_215316. Glutathione conjugation.

    Miscellaneous databases

    EvolutionaryTracei P10648.
    NextBioi 287091.
    PROi P10648.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_GSTA2.
    Genevestigatori P10648.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR003080. GST_alpha.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01266. GSTRNSFRASEA.
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Tissue-specific induction of murine glutathione transferase mRNAs by butylated hydroxyanisole."
      Pearson W.R., Reinhart J., Sisk S.C., Anderson K.S., Adler P.N.
      J. Biol. Chem. 263:13324-13332(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    5. "Residues 207, 216, and 221 and the catalytic activity of mGSTA1-1 and mGSTA2-2 toward benzo[a]pyrene-(7R,8S)-diol-(9S,10R)-epoxide."
      Gu Y., Xiao B., Wargo H.L., Bucher M.H., Singh S.V., Ji X.
      Biochemistry 42:917-921(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH GLUTATHIONE AND SUBSTRATE ANALOG, SUBUNIT.

    Entry informationi

    Entry nameiGSTA2_MOUSE
    AccessioniPrimary (citable) accession number: P10648
    Secondary accession number(s): Q6P8Q1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 119 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3