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Protein

Cytochrome P450 2D26

Gene

Cyp2d26

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi446 – 4461Iron (heme axial ligand)

GO - Molecular functioni

GO - Biological processi

  • arachidonic acid metabolic process Source: GO_Central
  • exogenous drug catabolic process Source: GO_Central
  • female pregnancy Source: RGD
  • oxidation-reduction process Source: GO_Central
  • response to drug Source: RGD
  • xenobiotic metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_315621. Xenobiotics.
SABIO-RKP10634.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2D26 (EC:1.14.14.1)
Alternative name(s):
CYPIID26
Cytochrome P450-CMF2
Cytochrome P450-DB2
Debrisoquine 4-hydroxylase
Gene namesi
Name:Cyp2d26
Synonyms:Cyp2d-26, Cyp2d2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi2471. Cyp2d2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500Cytochrome P450 2D26PRO_0000051747Add
BLAST

Proteomic databases

PaxDbiP10634.
PRIDEiP10634.

PTM databases

PhosphoSiteiP10634.

Expressioni

Inductioni

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Gene expression databases

GenevisibleiP10634. RN.

Interactioni

Protein-protein interaction databases

IntActiP10634. 1 interaction.
MINTiMINT-4563997.
STRINGi10116.ENSRNOP00000012413.

Structurei

3D structure databases

ProteinModelPortaliP10634.
SMRiP10634. Positions 37-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036991.
HOVERGENiHBG015789.
InParanoidiP10634.
KOiK07414.
OMAiWVTEEAG.
OrthoDBiEOG7RBZ85.
PhylomeDBiP10634.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01686. EP450ICYP2D.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10634-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLIGDDLW AVVIFTAIFL LLVDLVHRHK FWTAHYPPGP VPLPGLGNLL
60 70 80 90 100
QVDFENMPYS LYKLRSRYGD VFSLQIAWKP VVVINGLKAV RELLVTYGED
110 120 130 140 150
TADRPLLPIY NHLGYGNKSK GVVLAPYGPE WREQRRFSVS TLRDFGVGKK
160 170 180 190 200
SLEQWVTEEA GHLCDTFAKE AEHPFNPSIL LSKAVSNVIA SLVYARRFEY
210 220 230 240 250
EDPFFNRMLK TLKESFGEDT GFMAEVLNAI PILLQIPGLP GKVFPKLNSF
260 270 280 290 300
IALVDKMLIE HKKSWDPAQP PRDMTDAFLA EMQKAKGNPE SSFNDENLRL
310 320 330 340 350
VVIDLFMAGM VTTSTTLSWA LLLMILHPDV QRRVHEEIDE VIGQVRRPEM
360 370 380 390 400
ADQARMPFTN AVIHEVQRFA DIVPTNIPHM TSRDIKFQGF LIPKGTTLIP
410 420 430 440 450
NLSSVLKDET VWEKPLRFHP EHFLDAQGNF VKHEAFMPFS AGRRACLGEP
460 470 480 490 500
LARMELFLFF TCLLQRFSFS VLAGRPRPST HGVYALPVTP QPYQLCAVAR
Length:500
Mass (Da):56,684
Last modified:March 1, 1992 - v2
Checksum:i23E99250734C2215
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti117 – 1171N → D in AAA41049 (PubMed:3190674).Curated
Sequence conflicti346 – 3461R → L in AAA41055 (PubMed:3582092).Curated
Sequence conflicti346 – 3461R → L no nucleotide entry (PubMed:2819073).Curated
Sequence conflicti358 – 3581F → L in AAA41055 (PubMed:3582092).Curated
Sequence conflicti358 – 3581F → L no nucleotide entry (PubMed:2819073).Curated
Sequence conflicti407 – 4071K → E in AAA41055 (PubMed:3582092).Curated
Sequence conflicti407 – 4071K → E no nucleotide entry (PubMed:2819073).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16655 mRNA. Translation: AAA41055.1.
M22330 mRNA. Translation: AAA41049.1.
X52027 Genomic DNA. Translation: CAA36269.1.
AB008423 mRNA. Translation: BAA23123.1.
BC078897 mRNA. Translation: AAH78897.1.
PIRiB26822.
RefSeqiNP_036862.1. NM_012730.1.
UniGeneiRn.91355.

Genome annotation databases

EnsembliENSRNOT00000012413; ENSRNOP00000012413; ENSRNOG00000008988.
GeneIDi25053.
KEGGirno:25053.
UCSCiRGD:2471. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16655 mRNA. Translation: AAA41055.1.
M22330 mRNA. Translation: AAA41049.1.
X52027 Genomic DNA. Translation: CAA36269.1.
AB008423 mRNA. Translation: BAA23123.1.
BC078897 mRNA. Translation: AAH78897.1.
PIRiB26822.
RefSeqiNP_036862.1. NM_012730.1.
UniGeneiRn.91355.

3D structure databases

ProteinModelPortaliP10634.
SMRiP10634. Positions 37-500.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP10634. 1 interaction.
MINTiMINT-4563997.
STRINGi10116.ENSRNOP00000012413.

Chemistry

BindingDBiP10634.
ChEMBLiCHEMBL2483.

PTM databases

PhosphoSiteiP10634.

Proteomic databases

PaxDbiP10634.
PRIDEiP10634.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012413; ENSRNOP00000012413; ENSRNOG00000008988.
GeneIDi25053.
KEGGirno:25053.
UCSCiRGD:2471. rat.

Organism-specific databases

CTDi25053.
RGDi2471. Cyp2d2.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036991.
HOVERGENiHBG015789.
InParanoidiP10634.
KOiK07414.
OMAiWVTEEAG.
OrthoDBiEOG7RBZ85.
PhylomeDBiP10634.
TreeFamiTF352043.

Enzyme and pathway databases

ReactomeiREACT_315621. Xenobiotics.
SABIO-RKP10634.

Miscellaneous databases

NextBioi605254.
PROiP10634.

Gene expression databases

GenevisibleiP10634. RN.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01686. EP450ICYP2D.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Debrisoquine 4-hydroxylase: characterization of a new P450 gene subfamily, regulation, chromosomal mapping, and molecular analysis of the DA rat polymorphism."
    Gonzalez F.J., Matsunaga T., Nagata K., Meyer U.A., Nebert D.W., Pastewka J., Kozak C.A., Gillette J., Gelboin H.V., Hardwick J.P.
    DNA 6:149-161(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The CYP2D gene subfamily: analysis of the molecular basis of the debrisoquine 4-hydroxylase deficiency in DA rats."
    Matsunaga E., Zanger U.M., Hardwick J.P., Gelboin H.V., Meyer U.A., Gonzalez F.J.
    Biochemistry 28:7349-7355(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Four species of cDNAs for cytochrome P450 isozymes immunorelated to P450C-M/F encode for members of P450IID subfamily, increasing the number of members within the subfamily."
    Ishida N., Tawaragi Y., Inuzuka C., Sugita O., Kubota I., Nakazato H., Noguchi T., Sassa S.
    Biochem. Biophys. Res. Commun. 156:681-688(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The rat P450 IID subfamily: complete sequences of four closely linked genes and evidence that gene conversions maintained sequence homogeneity at the heme-binding region of the cytochrome P450 active site."
    Matsunaga E., Umeno M., Gonzalez F.J.
    J. Mol. Evol. 30:155-169(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  5. "Expression of four rat CYP2D isoforms in Saccharomyces cerevisiae and their catalytic specificity."
    Wan J., Imaoka S., Chow T., Hiroi T., Yabusaki Y., Funae Y.
    Arch. Biochem. Biophys. 348:383-390(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiCP2DQ_RAT
AccessioniPrimary (citable) accession number: P10634
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: March 1, 1992
Last modified: June 24, 2015
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.