Cytochrome P450 2D26 - Rattus norvegicus (Rat)

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P10634 (CP2DQ_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 119. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cytochrome P450 2D26
EC=1.14.14.1

Alternative name(s):
CYPIID26
Cytochrome P450-CMF2
Cytochrome P450-DB2
Debrisoquine 4-hydroxylase

Gene names

Name:	Cyp2d26
Synonyms:	Cyp2d-26, Cyp2d2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O₂ = ROH + oxidized flavoprotein + H₂O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	female pregnancy Inferred from expression pattern PubMed 18342837. Source: RGD response to drug Inferred from direct assay PubMed 18617602. Source: RGD
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell intracellular membrane-bounded organelle Inferred from direct assay PubMed 16485119. Source: RGD
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from direct assay Ref.5. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 500

500

Cytochrome P450 2D26

PRO_0000051747

Sites

Metal binding

446

Iron (heme axial ligand)

Amino acid modifications

Modified residue

138

Phosphoserine By similarity

Experimental info

Sequence conflict

117

N → D in AAA41049. Ref.3

Sequence conflict

346

R → L in AAA41055. Ref.1

Sequence conflict

346

R → L no nucleotide entry Ref.2

Sequence conflict

358

F → L in AAA41055. Ref.1

Sequence conflict

358

F → L no nucleotide entry Ref.2

Sequence conflict

407

K → E in AAA41055. Ref.1

Sequence conflict

407

K → E no nucleotide entry Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P10634 [UniParc].

Last modified March 1, 1992. Version 2.
Checksum: 23E99250734C2215
Show »

FASTA

500

56,684

        10         20         30         40         50         60 
MGLLIGDDLW AVVIFTAIFL LLVDLVHRHK FWTAHYPPGP VPLPGLGNLL QVDFENMPYS 

        70         80         90        100        110        120 
LYKLRSRYGD VFSLQIAWKP VVVINGLKAV RELLVTYGED TADRPLLPIY NHLGYGNKSK 

       130        140        150        160        170        180 
GVVLAPYGPE WREQRRFSVS TLRDFGVGKK SLEQWVTEEA GHLCDTFAKE AEHPFNPSIL 

       190        200        210        220        230        240 
LSKAVSNVIA SLVYARRFEY EDPFFNRMLK TLKESFGEDT GFMAEVLNAI PILLQIPGLP 

       250        260        270        280        290        300 
GKVFPKLNSF IALVDKMLIE HKKSWDPAQP PRDMTDAFLA EMQKAKGNPE SSFNDENLRL 

       310        320        330        340        350        360 
VVIDLFMAGM VTTSTTLSWA LLLMILHPDV QRRVHEEIDE VIGQVRRPEM ADQARMPFTN 

       370        380        390        400        410        420 
AVIHEVQRFA DIVPTNIPHM TSRDIKFQGF LIPKGTTLIP NLSSVLKDET VWEKPLRFHP 

       430        440        450        460        470        480 
EHFLDAQGNF VKHEAFMPFS AGRRACLGEP LARMELFLFF TCLLQRFSFS VLAGRPRPST 

       490        500 
HGVYALPVTP QPYQLCAVAR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Debrisoquine 4-hydroxylase: characterization of a new P450 gene subfamily, regulation, chromosomal mapping, and molecular analysis of the DA rat polymorphism." Gonzalez F.J., Matsunaga T., Nagata K., Meyer U.A., Nebert D.W., Pastewka J., Kozak C.A., Gillette J., Gelboin H.V., Hardwick J.P. DNA 6:149-161(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The CYP2D gene subfamily: analysis of the molecular basis of the debrisoquine 4-hydroxylase deficiency in DA rats." Matsunaga E., Zanger U.M., Hardwick J.P., Gelboin H.V., Meyer U.A., Gonzalez F.J. Biochemistry 28:7349-7355(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Four species of cDNAs for cytochrome P450 isozymes immunorelated to P450C-M/F encode for members of P450IID subfamily, increasing the number of members within the subfamily." Ishida N., Tawaragi Y., Inuzuka C., Sugita O., Kubota I., Nakazato H., Noguchi T., Sassa S. Biochem. Biophys. Res. Commun. 156:681-688(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The rat P450 IID subfamily: complete sequences of four closely linked genes and evidence that gene conversions maintained sequence homogeneity at the heme-binding region of the cytochrome P450 active site." Matsunaga E., Umeno M., Gonzalez F.J. J. Mol. Evol. 30:155-169(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Liver.
[5]	"Expression of four rat CYP2D isoforms in Saccharomyces cerevisiae and their catalytic specificity." Wan J., Imaoka S., Chow T., Hiroi T., Yabusaki Y., Funae Y. Arch. Biochem. Biophys. 348:383-390(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M16655 mRNA. Translation: AAA41055.1. M22330 mRNA. Translation: AAA41049.1. X52027 Genomic DNA. Translation: CAA36269.1. AB008423 mRNA. Translation: BAA23123.1. BC078897 mRNA. Translation: AAH78897.1.
IPI	IPI00231473.
PIR	B26822.
RefSeq	NP_036862.1. NM_012730.1.
UniGene	Rn.91355.
3D structure databases
ProteinModelPortal	P10634.
SMR	P10634. Positions 37-500.
ModBase	Search...
Protein-protein interaction databases
IntAct	P10634. 1 interaction.
STRING	10116.ENSRNOP00000012413.
PTM databases
PhosphoSite	P10634.
Proteomic databases
PaxDb	P10634.
PRIDE	P10634.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000012413; ENSRNOP00000012413; ENSRNOG00000008988.
GeneID	25053.
KEGG	rno:25053.
UCSC	RGD:2471. rat.
Organism-specific databases
CTD	25053.
RGD	2471. Cyp2d2.
Phylogenomic databases
eggNOG	COG2124.
GeneTree	ENSGT00670000097712.
HOGENOM	HOG000036991.
HOVERGEN	HBG015789.
InParanoid	P10634.
KO	K07414.
OrthoDB	EOG40VVPH.
Enzyme and pathway databases
SABIO-RK	P10634.
Gene expression databases
Genevestigator	P10634.
GermOnline	ENSRNOG00000008988. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008069. Cyt_P450_E_grp-I_CYP2D-like. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01686. EP450ICYP2D. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P10634.
ChEMBL	CHEMBL2483.
NextBio	605254.

Entry information

Entry name

CP2DQ_RAT

Accession

Primary (citable) accession number: P10634

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	March 1, 1992
Last modified:	April 3, 2013
This is version 119 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents