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P10634 (CP2DQ_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2D26

EC=1.14.14.1
Alternative name(s):
CYPIID26
Cytochrome P450-CMF2
Cytochrome P450-DB2
Debrisoquine 4-hydroxylase
Gene names
Name:Cyp2d26
Synonyms:Cyp2d-26, Cyp2d2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Induction

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 500500Cytochrome P450 2D26
PRO_0000051747

Sites

Metal binding4461Iron (heme axial ligand)

Experimental info

Sequence conflict1171N → D in AAA41049. Ref.3
Sequence conflict3461R → L in AAA41055. Ref.1
Sequence conflict3461R → L no nucleotide entry Ref.2
Sequence conflict3581F → L in AAA41055. Ref.1
Sequence conflict3581F → L no nucleotide entry Ref.2
Sequence conflict4071K → E in AAA41055. Ref.1
Sequence conflict4071K → E no nucleotide entry Ref.2

Sequences

Sequence LengthMass (Da)Tools
P10634 [UniParc].

Last modified March 1, 1992. Version 2.
Checksum: 23E99250734C2215

FASTA50056,684
        10         20         30         40         50         60 
MGLLIGDDLW AVVIFTAIFL LLVDLVHRHK FWTAHYPPGP VPLPGLGNLL QVDFENMPYS 

        70         80         90        100        110        120 
LYKLRSRYGD VFSLQIAWKP VVVINGLKAV RELLVTYGED TADRPLLPIY NHLGYGNKSK 

       130        140        150        160        170        180 
GVVLAPYGPE WREQRRFSVS TLRDFGVGKK SLEQWVTEEA GHLCDTFAKE AEHPFNPSIL 

       190        200        210        220        230        240 
LSKAVSNVIA SLVYARRFEY EDPFFNRMLK TLKESFGEDT GFMAEVLNAI PILLQIPGLP 

       250        260        270        280        290        300 
GKVFPKLNSF IALVDKMLIE HKKSWDPAQP PRDMTDAFLA EMQKAKGNPE SSFNDENLRL 

       310        320        330        340        350        360 
VVIDLFMAGM VTTSTTLSWA LLLMILHPDV QRRVHEEIDE VIGQVRRPEM ADQARMPFTN 

       370        380        390        400        410        420 
AVIHEVQRFA DIVPTNIPHM TSRDIKFQGF LIPKGTTLIP NLSSVLKDET VWEKPLRFHP 

       430        440        450        460        470        480 
EHFLDAQGNF VKHEAFMPFS AGRRACLGEP LARMELFLFF TCLLQRFSFS VLAGRPRPST 

       490        500 
HGVYALPVTP QPYQLCAVAR 

« Hide

References

« Hide 'large scale' references
[1]"Debrisoquine 4-hydroxylase: characterization of a new P450 gene subfamily, regulation, chromosomal mapping, and molecular analysis of the DA rat polymorphism."
Gonzalez F.J., Matsunaga T., Nagata K., Meyer U.A., Nebert D.W., Pastewka J., Kozak C.A., Gillette J., Gelboin H.V., Hardwick J.P.
DNA 6:149-161(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The CYP2D gene subfamily: analysis of the molecular basis of the debrisoquine 4-hydroxylase deficiency in DA rats."
Matsunaga E., Zanger U.M., Hardwick J.P., Gelboin H.V., Meyer U.A., Gonzalez F.J.
Biochemistry 28:7349-7355(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Four species of cDNAs for cytochrome P450 isozymes immunorelated to P450C-M/F encode for members of P450IID subfamily, increasing the number of members within the subfamily."
Ishida N., Tawaragi Y., Inuzuka C., Sugita O., Kubota I., Nakazato H., Noguchi T., Sassa S.
Biochem. Biophys. Res. Commun. 156:681-688(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"The rat P450 IID subfamily: complete sequences of four closely linked genes and evidence that gene conversions maintained sequence homogeneity at the heme-binding region of the cytochrome P450 active site."
Matsunaga E., Umeno M., Gonzalez F.J.
J. Mol. Evol. 30:155-169(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[5]"Expression of four rat CYP2D isoforms in Saccharomyces cerevisiae and their catalytic specificity."
Wan J., Imaoka S., Chow T., Hiroi T., Yabusaki Y., Funae Y.
Arch. Biochem. Biophys. 348:383-390(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M16655 mRNA. Translation: AAA41055.1.
M22330 mRNA. Translation: AAA41049.1.
X52027 Genomic DNA. Translation: CAA36269.1.
AB008423 mRNA. Translation: BAA23123.1.
BC078897 mRNA. Translation: AAH78897.1.
PIRB26822.
RefSeqNP_036862.1. NM_012730.1.
UniGeneRn.91355.

3D structure databases

ProteinModelPortalP10634.
SMRP10634. Positions 37-500.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP10634. 1 interaction.
MINTMINT-4563997.
STRING10116.ENSRNOP00000012413.

Chemistry

BindingDBP10634.
ChEMBLCHEMBL2483.

PTM databases

PhosphoSiteP10634.

Proteomic databases

PaxDbP10634.
PRIDEP10634.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000012413; ENSRNOP00000012413; ENSRNOG00000008988.
GeneID25053.
KEGGrno:25053.
UCSCRGD:2471. rat.

Organism-specific databases

CTD25053.
RGD2471. Cyp2d2.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00750000117392.
HOGENOMHOG000036991.
HOVERGENHBG015789.
InParanoidP10634.
KOK07414.
OMAWVTEEAG.
OrthoDBEOG7RBZ85.
PhylomeDBP10634.
TreeFamTF352043.

Enzyme and pathway databases

SABIO-RKP10634.

Gene expression databases

GenevestigatorP10634.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01686. EP450ICYP2D.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio605254.

Entry information

Entry nameCP2DQ_RAT
AccessionPrimary (citable) accession number: P10634
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: March 1, 1992
Last modified: June 11, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families