Cytochrome P450 2D1 - Rattus norvegicus (Rat)

Contribute

Send feedback

Read comments (?) or add your own

P10633 (CP2D1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cytochrome P450 2D1
EC=1.14.14.1

Alternative name(s):
CYPIID1
Cytochrome P450-CMF1A
Cytochrome P450-DB1
Cytochrome P450-UT-7
Debrisoquine 4-hydroxylase

Gene names

Name:	Cyp2d1
Synonyms:	Cyp2d-1, Cyp2d9

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O₂ = ROH + oxidized flavoprotein + H₂O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to drug Inferred from direct assay PubMed 18445370. Source: RGD response to organic cyclic compound Inferred from expression pattern PubMed 18197273. Source: RGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 17059882. Source: RGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from direct assay Ref.4. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 504

504

Cytochrome P450 2D1

PRO_0000051728

Sites

Metal binding

446

Iron (heme axial ligand)

Experimental info

Sequence conflict

123 – 124

IL → VF in AAA41043. Ref.3

Sequence conflict

173

Q → R in AAA41043. Ref.3

Sequence conflict

380

F → I in AAA41043. Ref.3

Sequence conflict

380

F → I in BAA23122. Ref.4

Sequence conflict

380

F → I in AAH78696. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P10633 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 2F9AD87B4EF327DC
Show »

FASTA

504

57,175

        10         20         30         40         50         60 
MELLNGTGLW SMAIFTVIFI LLVDLMHRRH RWTSRYPPGP VPWPVLGNLL QVDLSNMPYS 

        70         80         90        100        110        120 
LYKLQHRYGD VFSLQKGWKP MVIVNRLKAV QEVLVTHGED TADRPPVPIF KCLGVKPRSQ 

       130        140        150        160        170        180 
GVILASYGPE WREQRRFSVS TLRTFGMGKK SLEEWVTKEA GHLCDAFTAQ AGQSINPKAM 

       190        200        210        220        230        240 
LNKALCNVIA SLIFARRFEY EDPYLIRMVK LVEESLTEVS GFIPEVLNTF PALLRIPGLA 

       250        260        270        280        290        300 
DKVFQGQKTF MALLDNLLAE NRTTWDPAQP PRNLTDAFLA EVEKAKGNPE SSFNDENLRM 

       310        320        330        340        350        360 
VVVDLFTAGM VTTATTLTWA LLLMILYPDV QRRVQQEIDE VIGQVRCPEM TDQAHMPYTN 

       370        380        390        400        410        420 
AVIHEVQRFG DIAPLNLPRF TSCDIEVQDF VIPKGTTLII NLSSVLKDET VWEKPHRFHP 

       430        440        450        460        470        480 
EHFLDAQGNF VKHEAFMPFS AGRRACLGEP LARMELFLFF TCLLQRFSFS VPVGQPRPST 

       490        500 
HGFFAFPVAP LPYQLCAVVR EQGL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Debrisoquine 4-hydroxylase: characterization of a new P450 gene subfamily, regulation, chromosomal mapping, and molecular analysis of the DA rat polymorphism." Gonzalez F.J., Matsunaga T., Nagata K., Meyer U.A., Nebert D.W., Pastewka J., Kozak C.A., Gillette J., Gelboin H.V., Hardwick J.P. DNA 6:149-161(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The CYP2D gene subfamily: analysis of the molecular basis of the debrisoquine 4-hydroxylase deficiency in DA rats." Matsunaga E., Zanger U.M., Hardwick J.P., Gelboin H.V., Meyer U.A., Gonzalez F.J. Biochemistry 28:7349-7355(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Four species of cDNAs for cytochrome P450 isozymes immunorelated to P450C-M/F encode for members of P450IID subfamily, increasing the number of members within the subfamily." Ishida N., Tawaragi Y., Inuzuka C., Sugita O., Kubota I., Nakazato H., Noguchi T., Sassa S. Biochem. Biophys. Res. Commun. 156:681-688(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Expression of four rat CYP2D isoforms in Saccharomyces cerevisiae and their catalytic specificity." Wan J., Imaoka S., Chow T., Hiroi T., Yabusaki Y., Funae Y. Arch. Biochem. Biophys. 348:383-390(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M16654 mRNA. Translation: AAA41054.1. J02867 mRNA. Translation: AAA41001.1. M22328 mRNA. Translation: AAA41043.1. AB008422 mRNA. Translation: BAA23122.1. BC078696 mRNA. Translation: AAH78696.1.
IPI	IPI00324443.
PIR	A26822.
RefSeq	NP_695225.1. NM_153313.1.
UniGene	Rn.91133.
3D structure databases
ProteinModelPortal	P10633.
SMR	P10633. Positions 37-500.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000041174.
PTM databases
PhosphoSite	P10633.
Proteomic databases
PaxDb	P10633.
PRIDE	P10633.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000050002; ENSRNOP00000041174; ENSRNOG00000030182.
GeneID	266684.
KEGG	rno:266684.
UCSC	RGD:708427. rat.
Organism-specific databases
CTD	266684.
RGD	708427. Cyp2d1.
Phylogenomic databases
eggNOG	COG2124.
GeneTree	ENSGT00670000097712.
HOGENOM	HOG000036991.
HOVERGEN	HBG015789.
InParanoid	P10633.
KO	K07414.
OrthoDB	EOG40VVPH.
Enzyme and pathway databases
SABIO-RK	P10633.
Gene expression databases
Genevestigator	P10633.
GermOnline	ENSRNOG00000030182. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008069. Cyt_P450_E_grp-I_CYP2D-like. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01686. EP450ICYP2D. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P10633.
ChEMBL	CHEMBL2475.
NextBio	624484.

Entry information

Entry name

CP2D1_RAT

Accession

Primary (citable) accession number: P10633
Secondary accession number(s): O35105, Q6AZ78

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents