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P10633

- CP2D1_RAT

UniProt

P10633 - CP2D1_RAT

Protein

Cytochrome P450 2D1

Gene

Cyp2d1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi446 – 4461Iron (heme axial ligand)

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. monooxygenase activity Source: RGD

    GO - Biological processi

    1. response to drug Source: RGD
    2. response to organic cyclic compound Source: RGD
    3. response to organic substance Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    SABIO-RKP10633.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2D1 (EC:1.14.14.1)
    Alternative name(s):
    CYPIID1
    Cytochrome P450-CMF1A
    Cytochrome P450-DB1
    Cytochrome P450-UT-7
    Debrisoquine 4-hydroxylase
    Gene namesi
    Name:Cyp2d1
    Synonyms:Cyp2d-1, Cyp2d9
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 7

    Organism-specific databases

    RGDi708427. Cyp2d1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 504504Cytochrome P450 2D1PRO_0000051728Add
    BLAST

    Proteomic databases

    PaxDbiP10633.
    PRIDEiP10633.

    PTM databases

    PhosphoSiteiP10633.

    Expressioni

    Inductioni

    P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

    Gene expression databases

    GenevestigatoriP10633.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4564454.
    STRINGi10116.ENSRNOP00000041174.

    Structurei

    3D structure databases

    ProteinModelPortaliP10633.
    SMRiP10633. Positions 37-500.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00750000117392.
    HOGENOMiHOG000036991.
    HOVERGENiHBG015789.
    InParanoidiP10633.
    KOiK07414.
    OrthoDBiEOG7RBZ85.
    PhylomeDBiP10633.
    TreeFamiTF352043.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01686. EP450ICYP2D.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P10633-1 [UniParc]FASTAAdd to Basket

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    MELLNGTGLW SMAIFTVIFI LLVDLMHRRH RWTSRYPPGP VPWPVLGNLL    50
    QVDLSNMPYS LYKLQHRYGD VFSLQKGWKP MVIVNRLKAV QEVLVTHGED 100
    TADRPPVPIF KCLGVKPRSQ GVILASYGPE WREQRRFSVS TLRTFGMGKK 150
    SLEEWVTKEA GHLCDAFTAQ AGQSINPKAM LNKALCNVIA SLIFARRFEY 200
    EDPYLIRMVK LVEESLTEVS GFIPEVLNTF PALLRIPGLA DKVFQGQKTF 250
    MALLDNLLAE NRTTWDPAQP PRNLTDAFLA EVEKAKGNPE SSFNDENLRM 300
    VVVDLFTAGM VTTATTLTWA LLLMILYPDV QRRVQQEIDE VIGQVRCPEM 350
    TDQAHMPYTN AVIHEVQRFG DIAPLNLPRF TSCDIEVQDF VIPKGTTLII 400
    NLSSVLKDET VWEKPHRFHP EHFLDAQGNF VKHEAFMPFS AGRRACLGEP 450
    LARMELFLFF TCLLQRFSFS VPVGQPRPST HGFFAFPVAP LPYQLCAVVR 500
    EQGL 504
    Length:504
    Mass (Da):57,175
    Last modified:July 1, 1989 - v1
    Checksum:i2F9AD87B4EF327DC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti123 – 1242IL → VF in AAA41043. (PubMed:3190674)Curated
    Sequence conflicti173 – 1731Q → R in AAA41043. (PubMed:3190674)Curated
    Sequence conflicti380 – 3801F → I in AAA41043. (PubMed:3190674)Curated
    Sequence conflicti380 – 3801F → I in BAA23122. (PubMed:9434752)Curated
    Sequence conflicti380 – 3801F → I in AAH78696. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16654 mRNA. Translation: AAA41054.1.
    J02867 mRNA. Translation: AAA41001.1.
    M22328 mRNA. Translation: AAA41043.1.
    AB008422 mRNA. Translation: BAA23122.1.
    BC078696 mRNA. Translation: AAH78696.1.
    PIRiA26822.
    RefSeqiNP_695225.1. NM_153313.1.
    UniGeneiRn.91133.

    Genome annotation databases

    EnsembliENSRNOT00000050002; ENSRNOP00000041174; ENSRNOG00000030182.
    GeneIDi266684.
    KEGGirno:266684.
    UCSCiRGD:708427. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16654 mRNA. Translation: AAA41054.1 .
    J02867 mRNA. Translation: AAA41001.1 .
    M22328 mRNA. Translation: AAA41043.1 .
    AB008422 mRNA. Translation: BAA23122.1 .
    BC078696 mRNA. Translation: AAH78696.1 .
    PIRi A26822.
    RefSeqi NP_695225.1. NM_153313.1.
    UniGenei Rn.91133.

    3D structure databases

    ProteinModelPortali P10633.
    SMRi P10633. Positions 37-500.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4564454.
    STRINGi 10116.ENSRNOP00000041174.

    Chemistry

    BindingDBi P10633.
    ChEMBLi CHEMBL2475.

    PTM databases

    PhosphoSitei P10633.

    Proteomic databases

    PaxDbi P10633.
    PRIDEi P10633.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000050002 ; ENSRNOP00000041174 ; ENSRNOG00000030182 .
    GeneIDi 266684.
    KEGGi rno:266684.
    UCSCi RGD:708427. rat.

    Organism-specific databases

    CTDi 266684.
    RGDi 708427. Cyp2d1.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00750000117392.
    HOGENOMi HOG000036991.
    HOVERGENi HBG015789.
    InParanoidi P10633.
    KOi K07414.
    OrthoDBi EOG7RBZ85.
    PhylomeDBi P10633.
    TreeFami TF352043.

    Enzyme and pathway databases

    SABIO-RK P10633.

    Miscellaneous databases

    NextBioi 624484.

    Gene expression databases

    Genevestigatori P10633.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01686. EP450ICYP2D.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Debrisoquine 4-hydroxylase: characterization of a new P450 gene subfamily, regulation, chromosomal mapping, and molecular analysis of the DA rat polymorphism."
      Gonzalez F.J., Matsunaga T., Nagata K., Meyer U.A., Nebert D.W., Pastewka J., Kozak C.A., Gillette J., Gelboin H.V., Hardwick J.P.
      DNA 6:149-161(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The CYP2D gene subfamily: analysis of the molecular basis of the debrisoquine 4-hydroxylase deficiency in DA rats."
      Matsunaga E., Zanger U.M., Hardwick J.P., Gelboin H.V., Meyer U.A., Gonzalez F.J.
      Biochemistry 28:7349-7355(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Four species of cDNAs for cytochrome P450 isozymes immunorelated to P450C-M/F encode for members of P450IID subfamily, increasing the number of members within the subfamily."
      Ishida N., Tawaragi Y., Inuzuka C., Sugita O., Kubota I., Nakazato H., Noguchi T., Sassa S.
      Biochem. Biophys. Res. Commun. 156:681-688(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Expression of four rat CYP2D isoforms in Saccharomyces cerevisiae and their catalytic specificity."
      Wan J., Imaoka S., Chow T., Hiroi T., Yabusaki Y., Funae Y.
      Arch. Biochem. Biophys. 348:383-390(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiCP2D1_RAT
    AccessioniPrimary (citable) accession number: P10633
    Secondary accession number(s): O35105, Q6AZ78
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3