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Protein

Eukaryotic initiation factor 4A-II

Gene

Eif4a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 84ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-1169408. ISG15 antiviral mechanism.
R-MMU-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-MMU-429947. Deadenylation of mRNA.
R-MMU-72649. Translation initiation complex formation.
R-MMU-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-MMU-72702. Ribosomal scanning and start codon recognition.
R-MMU-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic initiation factor 4A-II (EC:3.6.4.13)
Short name:
eIF-4A-II
Short name:
eIF4A-II
Alternative name(s):
ATP-dependent RNA helicase eIF4A-2
Gene namesi
Name:Eif4a2
Synonyms:Ddx2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:106906. Eif4a2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000549401 – 407Eukaryotic initiation factor 4A-IIAdd BLAST407

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei159PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP10630.
PaxDbiP10630.
PeptideAtlasiP10630.
PRIDEiP10630.

PTM databases

iPTMnetiP10630.
PhosphoSitePlusiP10630.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022884.
CleanExiMM_EIF4A2.
ExpressionAtlasiP10630. baseline and differential.
GenevisibleiP10630. MM.

Interactioni

Subunit structurei

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIFFG3. Interacts with EIF4E. May interact with NOM1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi199419. 6 interactors.
IntActiP10630. 4 interactors.
MINTiMINT-1869954.
STRINGi10090.ENSMUSP00000023599.

Structurei

3D structure databases

ProteinModelPortaliP10630.
SMRiP10630.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini64 – 235Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST172
Domaini246 – 407Helicase C-terminalPROSITE-ProRule annotationAdd BLAST162

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi33 – 61Q motifAdd BLAST29
Motifi183 – 186DEAD box4

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0327. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00530000062880.
HOGENOMiHOG000268797.
HOVERGENiHBG107989.
InParanoidiP10630.
KOiK03257.
OMAiNEITDNF.
OrthoDBiEOG091G07OI.
PhylomeDBiP10630.
TreeFamiTF101524.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR031258. EIF4A1/2.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PANTHERiPTHR24031:SF226. PTHR24031:SF226. 1 hit.
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P10630-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGGSADYNR EHGGPEGMDP DGVIESNWNE IVDNFDDMNL KESLLRGIYA
60 70 80 90 100
YGFEKPSAIQ QRAIIPCIKG YDVIAQAQSG TGKTATFAIS ILQQLEIEFK
110 120 130 140 150
ETQALVLAPT RELAQQIQKV ILALGDYMGA TCHACIGGTN VRNEMQKLQA
160 170 180 190 200
EAPHIVVGTP GRVFDMLNRR YLSPKWIKMF VLDEADEMLS RGFKDQIYEI
210 220 230 240 250
FQKLNTSIQV VLLSATMPTD VLEVTKKFMR DPIRILVKKE ELTLEGIKQF
260 270 280 290 300
YINVEREEWK LDTLCDLYET LTITQAVIFL NTRRKVDWLT EKMHARDFTV
310 320 330 340 350
SALHGDMDQK ERDVIMREFR SGSSRVLITT DLLARGIDVQ QVSLVINYDL
360 370 380 390 400
PTNRENYIHR IGRGGRFGRK GVAINFVTEE DKRILRDIET FYNTTVEEMP

MNVADLI
Length:407
Mass (Da):46,402
Last modified:November 1, 1997 - v2
Checksum:iFAA7D3BA9D8C6DA0
GO
Isoform 2 (identifier: P10630-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     9-9: N → NS

Show »
Length:408
Mass (Da):46,489
Checksum:i0F47C0C4EEF00DD3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti200 – 201IF → RV in CAA31025 (PubMed:3046931).Curated2
Sequence conflicti294 – 297HARD → QAIY in CAA31025 (PubMed:3046931).Curated4
Sequence conflicti387D → H in CAA31025 (PubMed:3046931).Curated1
Sequence conflicti403V → G in CAA31025 (PubMed:3046931).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0096309N → NS in isoform 2. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12507 mRNA. Translation: CAA31025.1.
X14422 Genomic DNA. Translation: CAA32585.1.
X56953 Genomic DNA. Translation: CAA40269.1.
X56953 Genomic DNA. Translation: CAA40268.1.
AK076509 mRNA. Translation: BAC36372.1.
U64706 Genomic DNA. Translation: AAC53180.1.
CCDSiCCDS28074.1. [P10630-1]
PIRiS00985.
RefSeqiNP_001116509.1. NM_001123037.2.
NP_001116510.1. NM_001123038.2.
NP_038534.2. NM_013506.3. [P10630-1]
XP_006521846.1. XM_006521783.3. [P10630-2]
UniGeneiMm.260084.

Genome annotation databases

EnsembliENSMUST00000023599; ENSMUSP00000023599; ENSMUSG00000022884. [P10630-1]
ENSMUST00000115341; ENSMUSP00000110998; ENSMUSG00000022884. [P10630-2]
GeneIDi13682.
KEGGimmu:13682.
UCSCiuc007ytd.3. mouse. [P10630-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12507 mRNA. Translation: CAA31025.1.
X14422 Genomic DNA. Translation: CAA32585.1.
X56953 Genomic DNA. Translation: CAA40269.1.
X56953 Genomic DNA. Translation: CAA40268.1.
AK076509 mRNA. Translation: BAC36372.1.
U64706 Genomic DNA. Translation: AAC53180.1.
CCDSiCCDS28074.1. [P10630-1]
PIRiS00985.
RefSeqiNP_001116509.1. NM_001123037.2.
NP_001116510.1. NM_001123038.2.
NP_038534.2. NM_013506.3. [P10630-1]
XP_006521846.1. XM_006521783.3. [P10630-2]
UniGeneiMm.260084.

3D structure databases

ProteinModelPortaliP10630.
SMRiP10630.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199419. 6 interactors.
IntActiP10630. 4 interactors.
MINTiMINT-1869954.
STRINGi10090.ENSMUSP00000023599.

PTM databases

iPTMnetiP10630.
PhosphoSitePlusiP10630.

Proteomic databases

EPDiP10630.
PaxDbiP10630.
PeptideAtlasiP10630.
PRIDEiP10630.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023599; ENSMUSP00000023599; ENSMUSG00000022884. [P10630-1]
ENSMUST00000115341; ENSMUSP00000110998; ENSMUSG00000022884. [P10630-2]
GeneIDi13682.
KEGGimmu:13682.
UCSCiuc007ytd.3. mouse. [P10630-1]

Organism-specific databases

CTDi1974.
MGIiMGI:106906. Eif4a2.

Phylogenomic databases

eggNOGiKOG0327. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00530000062880.
HOGENOMiHOG000268797.
HOVERGENiHBG107989.
InParanoidiP10630.
KOiK03257.
OMAiNEITDNF.
OrthoDBiEOG091G07OI.
PhylomeDBiP10630.
TreeFamiTF101524.

Enzyme and pathway databases

ReactomeiR-MMU-1169408. ISG15 antiviral mechanism.
R-MMU-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-MMU-429947. Deadenylation of mRNA.
R-MMU-72649. Translation initiation complex formation.
R-MMU-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-MMU-72702. Ribosomal scanning and start codon recognition.
R-MMU-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

PROiP10630.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022884.
CleanExiMM_EIF4A2.
ExpressionAtlasiP10630. baseline and differential.
GenevisibleiP10630. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR031258. EIF4A1/2.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PANTHERiPTHR24031:SF226. PTHR24031:SF226. 1 hit.
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF4A2_MOUSE
AccessioniPrimary (citable) accession number: P10630
Secondary accession number(s): Q61513, Q61514
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.