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Protein

Microsomal glutathione S-transferase 1

Gene

MGST1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Enzyme regulationi

Can be activated by reagents that attack Cys-50 sulfhydryl, such as N-ethylmaleimide. Activation also occurs via nitration of Tyr-93 by peroxynitrite (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38GlutathioneBy similarity1
Sitei50Activates the enzyme when modifiedBy similarity1
Binding sitei73GlutathioneBy similarity1
Binding sitei74GlutathioneBy similarity1
Binding sitei76GlutathioneBy similarity1
Binding sitei81GlutathioneBy similarity1
Binding sitei121GlutathioneBy similarity1

GO - Molecular functioni

  • glutathione binding Source: Ensembl
  • glutathione peroxidase activity Source: UniProtKB
  • glutathione transferase activity Source: UniProtKB
  • protein homodimerization activity Source: Ensembl

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

ReactomeiR-HSA-156590 Glutathione conjugation
R-HSA-5423646 Aflatoxin activation and detoxification
R-HSA-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Microsomal glutathione S-transferase 1 (EC:2.5.1.18)
Short name:
Microsomal GST-1
Alternative name(s):
Microsomal GST-I
Gene namesi
Name:MGST1
Synonyms:GST12, MGST
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000008394.12
HGNCiHGNC:7061 MGST1
MIMi138330 gene
neXtProtiNX_P10620

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini3 – 9LumenalBy similarity7
Transmembranei10 – 33HelicalBy similarityAdd BLAST24
Topological domaini34 – 62CytoplasmicBy similarityAdd BLAST29
Transmembranei63 – 96HelicalBy similarityAdd BLAST34
Topological domaini97 – 99LumenalBy similarity3
Transmembranei100 – 123HelicalBy similarityAdd BLAST24
Topological domaini124 – 128CytoplasmicBy similarity5
Transmembranei129 – 148HelicalBy similarityAdd BLAST20
Topological domaini149 – 155LumenalBy similarity7

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi4257
OpenTargetsiENSG00000008394
PharmGKBiPA30791

Chemistry databases

ChEMBLiCHEMBL1743184
DrugBankiDB00143 Glutathione

Polymorphism and mutation databases

BioMutaiMGST1
DMDMi121740

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002177361 – 155Microsomal glutathione S-transferase 1Add BLAST155

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei42N6-acetyllysineBy similarity1
Modified residuei55N6-acetyllysineBy similarity1
Modified residuei60N6-acetyllysineBy similarity1

Post-translational modificationi

Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.By similarity

Keywords - PTMi

Acetylation, Nitration

Proteomic databases

EPDiP10620
MaxQBiP10620
PaxDbiP10620
PeptideAtlasiP10620
PRIDEiP10620
TopDownProteomicsiP10620-1 [P10620-1]

PTM databases

iPTMnetiP10620
PhosphoSitePlusiP10620
SwissPalmiP10620

Expressioni

Tissue specificityi

Highly expressed in liver.

Gene expression databases

BgeeiENSG00000008394
CleanExiHS_MGST1
ExpressionAtlasiP10620 baseline and differential
GenevisibleiP10620 HS

Organism-specific databases

HPAiHPA044840

Interactioni

Subunit structurei

Homotrimer; The trimer binds only one molecule of glutathione.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
NELFBQ8WX922EBI-2691601,EBI-347721

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110413, 7 interactors
IntActiP10620, 8 interactors
MINTiP10620
STRINGi9606.ENSP00000010404

Structurei

3D structure databases

ProteinModelPortaliP10620
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MAPEG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IXE1 Eukaryota
ENOG4111VJG LUCA
GeneTreeiENSGT00390000011980
HOGENOMiHOG000231759
HOVERGENiHBG052470
InParanoidiP10620
KOiK00799
OMAiFYRMTRK
OrthoDBiEOG091G14I4
PhylomeDBiP10620
TreeFamiTF105327

Family and domain databases

Gene3Di1.20.120.550, 1 hit
InterProiView protein in InterPro
IPR023352 MAPEG-like_dom_sf
IPR001129 Membr-assoc_MAPEG
PfamiView protein in Pfam
PF01124 MAPEG, 1 hit
SUPFAMiSSF161084 SSF161084, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P10620-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVDLTQVMDD EVFMAFASYA TIILSKMMLM STATAFYRLT RKVFANPEDC
60 70 80 90 100
VAFGKGENAK KYLRTDDRVE RVRRAHLNDL ENIIPFLGIG LLYSLSGPDP
110 120 130 140 150
STAILHFRLF VGARIYHTIA YLTPLPQPNR ALSFFVGYGV TLSMAYRLLK

SKLYL
Length:155
Mass (Da):17,599
Last modified:July 1, 1989 - v1
Checksum:i892A529C97E3C853
GO
Isoform 2 (identifier: P10620-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     75-155: AHLNDLENII...YRLLKSKLYL → IKQTLSIYLASSI

Note: No experimental confirmation available.
Show »
Length:87
Mass (Da):9,984
Checksum:i8F4C9AF76F18EF97
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04616075 – 155AHLND…SKLYL → IKQTLSIYLASSI in isoform 2. 1 PublicationAdd BLAST81

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03746 mRNA Translation: AAA35934.1
U46498, AF092926, U46497 Genomic DNA Translation: AAC50711.1
U71213, U71211, U71212 Genomic DNA Translation: AAB17184.1
BT006982 mRNA Translation: AAP35628.1
AY368173 Genomic DNA Translation: AAQ55111.1
AK291148 mRNA Translation: BAF83837.1
AC007528 Genomic DNA No translation available.
AC007529 Genomic DNA No translation available.
AC007552 Genomic DNA No translation available.
CH471094 Genomic DNA Translation: EAW96362.1
CH471094 Genomic DNA Translation: EAW96367.1
BC005923 mRNA Translation: AAH05923.1
BC056863 mRNA No translation available.
CCDSiCCDS58209.1 [P10620-2]
CCDS8677.1 [P10620-1]
PIRiB28083
RefSeqiNP_001247440.1, NM_001260511.1 [P10620-1]
NP_001247441.1, NM_001260512.1
NP_001254527.1, NM_001267598.1 [P10620-2]
NP_064696.1, NM_020300.4 [P10620-1]
NP_665707.1, NM_145764.2 [P10620-1]
NP_665734.1, NM_145791.2 [P10620-1]
NP_665735.1, NM_145792.2 [P10620-1]
UniGeneiHs.389700

Genome annotation databases

EnsembliENST00000010404; ENSP00000010404; ENSG00000008394 [P10620-1]
ENST00000396207; ENSP00000379510; ENSG00000008394 [P10620-1]
ENST00000396209; ENSP00000379512; ENSG00000008394 [P10620-1]
ENST00000396210; ENSP00000379513; ENSG00000008394 [P10620-1]
ENST00000535309; ENSP00000438308; ENSG00000008394 [P10620-2]
GeneIDi4257
KEGGihsa:4257
UCSCiuc001rdf.4 human [P10620-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMGST1_HUMAN
AccessioniPrimary (citable) accession number: P10620
Secondary accession number(s): A8K533, G5EA53
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: March 28, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health