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Protein

Microsomal glutathione S-transferase 1

Gene

MGST1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Enzyme regulationi

Can be activated by reagents that attack Cys-50 sulfhydryl, such as N-ethylmaleimide. Activation also occurs via nitration of Tyr-93 by peroxynitrite (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38GlutathioneBy similarity1
Sitei50Activates the enzyme when modifiedBy similarity1
Binding sitei73GlutathioneBy similarity1
Binding sitei74GlutathioneBy similarity1
Binding sitei76GlutathioneBy similarity1
Binding sitei81GlutathioneBy similarity1
Binding sitei121GlutathioneBy similarity1

GO - Molecular functioni

  • glutathione binding Source: Ensembl
  • glutathione peroxidase activity Source: UniProtKB
  • glutathione transferase activity Source: UniProtKB
  • protein homodimerization activity Source: Ensembl

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

ReactomeiR-HSA-156590. Glutathione conjugation.
R-HSA-5423646. Aflatoxin activation and detoxification.
R-HSA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Microsomal glutathione S-transferase 1 (EC:2.5.1.18)
Short name:
Microsomal GST-1
Alternative name(s):
Microsomal GST-I
Gene namesi
Name:MGST1
Synonyms:GST12, MGST
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000008394.12.
HGNCiHGNC:7061. MGST1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini3 – 9LumenalBy similarity7
Transmembranei10 – 33HelicalBy similarityAdd BLAST24
Topological domaini34 – 62CytoplasmicBy similarityAdd BLAST29
Transmembranei63 – 96HelicalBy similarityAdd BLAST34
Topological domaini97 – 99LumenalBy similarity3
Transmembranei100 – 123HelicalBy similarityAdd BLAST24
Topological domaini124 – 128CytoplasmicBy similarity5
Transmembranei129 – 148HelicalBy similarityAdd BLAST20
Topological domaini149 – 155LumenalBy similarity7

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi4257.
OpenTargetsiENSG00000008394.
PharmGKBiPA30791.

Chemistry databases

ChEMBLiCHEMBL1743184.
DrugBankiDB00143. Glutathione.

Polymorphism and mutation databases

BioMutaiMGST1.
DMDMi121740.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002177361 – 155Microsomal glutathione S-transferase 1Add BLAST155

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei42N6-acetyllysineBy similarity1
Modified residuei55N6-acetyllysineBy similarity1
Modified residuei60N6-acetyllysineBy similarity1

Post-translational modificationi

Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.By similarity

Keywords - PTMi

Acetylation, Nitration

Proteomic databases

EPDiP10620.
MaxQBiP10620.
PaxDbiP10620.
PeptideAtlasiP10620.
PRIDEiP10620.
TopDownProteomicsiP10620-1. [P10620-1]

PTM databases

iPTMnetiP10620.
PhosphoSitePlusiP10620.
SwissPalmiP10620.

Expressioni

Tissue specificityi

Highly expressed in liver.

Gene expression databases

BgeeiENSG00000008394.
CleanExiHS_MGST1.
ExpressionAtlasiP10620. baseline and differential.
GenevisibleiP10620. HS.

Organism-specific databases

HPAiHPA044840.

Interactioni

Subunit structurei

Homotrimer; The trimer binds only one molecule of glutathione.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
NELFBQ8WX922EBI-2691601,EBI-347721

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110413. 6 interactors.
IntActiP10620. 8 interactors.
STRINGi9606.ENSP00000010404.

Structurei

3D structure databases

ProteinModelPortaliP10620.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MAPEG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IXE1. Eukaryota.
ENOG4111VJG. LUCA.
GeneTreeiENSGT00390000011980.
HOGENOMiHOG000231759.
HOVERGENiHBG052470.
InParanoidiP10620.
KOiK00799.
OMAiFYRMTRK.
OrthoDBiEOG091G14I4.
PhylomeDBiP10620.
TreeFamiTF105327.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiView protein in InterPro
IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
PfamiView protein in Pfam
PF01124. MAPEG. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P10620-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVDLTQVMDD EVFMAFASYA TIILSKMMLM STATAFYRLT RKVFANPEDC
60 70 80 90 100
VAFGKGENAK KYLRTDDRVE RVRRAHLNDL ENIIPFLGIG LLYSLSGPDP
110 120 130 140 150
STAILHFRLF VGARIYHTIA YLTPLPQPNR ALSFFVGYGV TLSMAYRLLK

SKLYL
Length:155
Mass (Da):17,599
Last modified:July 1, 1989 - v1
Checksum:i892A529C97E3C853
GO
Isoform 2 (identifier: P10620-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     75-155: AHLNDLENII...YRLLKSKLYL → IKQTLSIYLASSI

Note: No experimental confirmation available.
Show »
Length:87
Mass (Da):9,984
Checksum:i8F4C9AF76F18EF97
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04616075 – 155AHLND…SKLYL → IKQTLSIYLASSI in isoform 2. 1 PublicationAdd BLAST81

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03746 mRNA. Translation: AAA35934.1.
U46498, AF092926, U46497 Genomic DNA. Translation: AAC50711.1.
U71213, U71211, U71212 Genomic DNA. Translation: AAB17184.1.
BT006982 mRNA. Translation: AAP35628.1.
AY368173 Genomic DNA. Translation: AAQ55111.1.
AK291148 mRNA. Translation: BAF83837.1.
AC007528 Genomic DNA. No translation available.
AC007529 Genomic DNA. No translation available.
AC007552 Genomic DNA. No translation available.
CH471094 Genomic DNA. Translation: EAW96362.1.
CH471094 Genomic DNA. Translation: EAW96367.1.
BC005923 mRNA. Translation: AAH05923.1.
BC056863 mRNA. No translation available.
CCDSiCCDS58209.1. [P10620-2]
CCDS8677.1. [P10620-1]
PIRiB28083.
RefSeqiNP_001247440.1. NM_001260511.1. [P10620-1]
NP_001247441.1. NM_001260512.1.
NP_001254527.1. NM_001267598.1. [P10620-2]
NP_064696.1. NM_020300.4. [P10620-1]
NP_665707.1. NM_145764.2. [P10620-1]
NP_665734.1. NM_145791.2. [P10620-1]
NP_665735.1. NM_145792.2. [P10620-1]
UniGeneiHs.389700.

Genome annotation databases

EnsembliENST00000010404; ENSP00000010404; ENSG00000008394. [P10620-1]
ENST00000396207; ENSP00000379510; ENSG00000008394. [P10620-1]
ENST00000396209; ENSP00000379512; ENSG00000008394. [P10620-1]
ENST00000396210; ENSP00000379513; ENSG00000008394. [P10620-1]
ENST00000535309; ENSP00000438308; ENSG00000008394. [P10620-2]
GeneIDi4257.
KEGGihsa:4257.
UCSCiuc001rdf.4. human. [P10620-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMGST1_HUMAN
AccessioniPrimary (citable) accession number: P10620
Secondary accession number(s): A8K533, G5EA53
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: September 27, 2017
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families