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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gap

Organism
Methanothermus fervidus
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits a dual-cofactor specificity, with a marked preference for NADP+ over NAD+.

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei110NADP; via amide nitrogen1 Publication1
Active sitei140NucleophileBy similarity1
Binding sitei171NADP1 Publication1
Binding sitei300NADP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 12NADP1 Publication2
Nucleotide bindingi34 – 35NADP1 Publication2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

SABIO-RKP10618.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase (EC:1.2.1.59)
Short name:
GAPDH
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase
Gene namesi
Name:gap
OrganismiMethanothermus fervidus
Taxonomic identifieri2180 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanothermaceaeMethanothermus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001457221 – 337Glyceraldehyde-3-phosphate dehydrogenaseAdd BLAST337

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi523846.Mfer_0276.

Structurei

Secondary structure

1337
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi13 – 21Combined sources9
Beta strandi23 – 35Combined sources13
Helixi38 – 45Combined sources8
Beta strandi50 – 54Combined sources5
Helixi55 – 57Combined sources3
Helixi58 – 63Combined sources6
Helixi72 – 77Combined sources6
Beta strandi80 – 84Combined sources5
Helixi90 – 101Combined sources12
Beta strandi105 – 107Combined sources3
Helixi113 – 116Combined sources4
Helixi122 – 125Combined sources4
Helixi126 – 129Combined sources4
Beta strandi133 – 137Combined sources5
Helixi140 – 156Combined sources17
Beta strandi158 – 170Combined sources13
Beta strandi184 – 191Combined sources8
Helixi194 – 199Combined sources6
Beta strandi206 – 214Combined sources9
Beta strandi219 – 229Combined sources11
Helixi233 – 242Combined sources10
Beta strandi246 – 249Combined sources4
Turni251 – 254Combined sources4
Helixi258 – 268Combined sources11
Helixi271 – 273Combined sources3
Beta strandi277 – 281Combined sources5
Helixi282 – 284Combined sources3
Beta strandi286 – 288Combined sources3
Beta strandi291 – 298Combined sources8
Turni300 – 303Combined sources4
Helixi304 – 315Combined sources12
Helixi322 – 333Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CF2X-ray2.10O/P/Q/R1-337[»]
ProteinModelPortaliP10618.
SMRiP10618.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10618.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni139 – 141Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni194 – 195Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00493. Archaea.
COG0057. LUCA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00559. G3P_dehdrog_arch. 1 hit.
InterProiIPR000846. DapB_N.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01113. DapB_N. 1 hit.
PF02800. Gp_dh_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10618-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAVAINGYG TVGKRVADAI AQQDDMKVIG VSKTRPDFEA RMALKKGYDL
60 70 80 90 100
YVAIPERVKL FEKAGIEVAG TVDDMLDEAD IVIDCTPEGI GAKNLKMYKE
110 120 130 140 150
KGIKAIFQGG EKHEDIGLSF NSLSNYEESY GKDYTRVVSC NTTGLCRTLK
160 170 180 190 200
PLHDSFGIKK VRAVIVRRGA DPAQVSKGPI NAIIPNPPKL PSHHGPDVKT
210 220 230 240 250
VLDINIDTMA VIVPTTLMHQ HNVMVEVEET PTVDDIIDVF EDTPRVILIS
260 270 280 290 300
AEDGLTSTAE IMEYAKELGR SRNDLFEIPV WRESITVVDN EIYYMQAVHQ
310 320 330
ESDIVPENVD AVRAILEMEE DKYKSINKTN KAMNILQ
Length:337
Mass (Da):37,408
Last modified:July 1, 1989 - v1
Checksum:iD83CBA88AAE5B0B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19980 Genomic DNA. Translation: AAA88227.1.
PIRiJT0286.
RefSeqiWP_013413357.1. NC_014658.1.

Genome annotation databases

GeneIDi9961991.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19980 Genomic DNA. Translation: AAA88227.1.
PIRiJT0286.
RefSeqiWP_013413357.1. NC_014658.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CF2X-ray2.10O/P/Q/R1-337[»]
ProteinModelPortaliP10618.
SMRiP10618.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi523846.Mfer_0276.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi9961991.

Phylogenomic databases

eggNOGiarCOG00493. Archaea.
COG0057. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.
SABIO-RKP10618.

Miscellaneous databases

EvolutionaryTraceiP10618.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00559. G3P_dehdrog_arch. 1 hit.
InterProiIPR000846. DapB_N.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01113. DapB_N. 1 hit.
PF02800. Gp_dh_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3P_METFE
AccessioniPrimary (citable) accession number: P10618
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.