Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P10614

- CP51_YEAST

UniProt

P10614 - CP51_YEAST

Protein

Lanosterol 14-alpha demethylase

Gene

ERG11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 143 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.

    Catalytic activityi

    A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.

    Cofactori

    Heme group.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi470 – 4701Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. protein binding Source: IntAct
    4. sterol 14-demethylase activity Source: SGD

    GO - Biological processi

    1. demethylation Source: GOC
    2. ergosterol biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:YHR007C-MONOMER.
    YEAST:YHR007C-MONOMER.
    UniPathwayiUPA00770; UER00754.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lanosterol 14-alpha demethylase (EC:1.14.13.70)
    Alternative name(s):
    CYPLI
    Cytochrome P450 51
    Cytochrome P450-14DM
    Cytochrome P450-LIA1
    Sterol 14-alpha demethylase
    Gene namesi
    Name:ERG11
    Synonyms:CYP51
    Ordered Locus Names:YHR007C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR007c.
    SGDiS000001049. ERG11.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 530530Lanosterol 14-alpha demethylasePRO_0000052012Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei458 – 4581Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP10614.
    PaxDbiP10614.
    PeptideAtlasiP10614.

    Expressioni

    Gene expression databases

    GenevestigatoriP10614.

    Interactioni

    Subunit structurei

    Interacts with ERG28.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ERG25P530453EBI-5127,EBI-6506

    Protein-protein interaction databases

    BioGridi36434. 238 interactions.
    DIPiDIP-7886N.
    IntActiP10614. 68 interactions.
    MINTiMINT-1325794.
    STRINGi4932.YHR007C.

    Structurei

    Secondary structure

    1
    530
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 2315
    Helixi27 – 4923
    Beta strandi53 – 553
    Turni64 – 663
    Helixi69 – 746
    Helixi76 – 8712
    Beta strandi89 – 957
    Beta strandi98 – 1036
    Helixi105 – 1139
    Turni117 – 1193
    Helixi122 – 13413
    Helixi144 – 15512
    Helixi160 – 18021
    Turni182 – 1843
    Turni186 – 1883
    Beta strandi190 – 1956
    Helixi196 – 21217
    Helixi215 – 2206
    Helixi225 – 23410
    Helixi238 – 2425
    Helixi249 – 27426
    Beta strandi280 – 2823
    Helixi283 – 2897
    Helixi301 – 33131
    Helixi334 – 34714
    Helixi349 – 3513
    Helixi357 – 3604
    Helixi364 – 37613
    Beta strandi383 – 3875
    Beta strandi405 – 4084
    Helixi410 – 4134
    Turni417 – 4193
    Turni421 – 4244
    Helixi428 – 4314
    Beta strandi444 – 4496
    Beta strandi451 – 4544
    Helixi466 – 4683
    Helixi473 – 49018
    Beta strandi491 – 4944
    Beta strandi507 – 5104
    Beta strandi518 – 5258

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4LXJX-ray1.90A1-530[»]
    ProteinModelPortaliP10614.
    SMRiP10614. Positions 6-530.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2020ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini42 – 530489CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei21 – 4121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00660000095370.
    HOGENOMiHOG000042780.
    KOiK05917.
    OMAiDFTSMVT.
    OrthoDBiEOG7712HB.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002403. Cyt_P450_E_grp-IV.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00465. EP450IV.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P10614-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSATKSIVGE ALEYVNIGLS HFLALPLAQR ISLIIIIPFI YNIVWQLLYS    50
    LRKDRPPLVF YWIPWVGSAV VYGMKPYEFF EECQKKYGDI FSFVLLGRVM 100
    TVYLGPKGHE FVFNAKLADV SAEAAYAHLT TPVFGKGVIY DCPNSRLMEQ 150
    KKFVKGALTK EAFKSYVPLI AEEVYKYFRD SKNFRLNERT TGTIDVMVTQ 200
    PEMTIFTASR SLLGKEMRAK LDTDFAYLYS DLDKGFTPIN FVFPNLPLEH 250
    YRKRDHAQKA ISGTYMSLIK ERRKNNDIQD RDLIDSLMKN STYKDGVKMT 300
    DQEIANLLIG VLMGGQHTSA ATSAWILLHL AERPDVQQEL YEEQMRVLDG 350
    GKKELTYDLL QEMPLLNQTI KETLRMHHPL HSLFRKVMKD MHVPNTSYVI 400
    PAGYHVLVSP GYTHLRDEYF PNAHQFNIHR WNKDSASSYS VGEEVDYGFG 450
    AISKGVSSPY LPFGGGRHRC IGEHFAYCQL GVLMSIFIRT LKWHYPEGKT 500
    VPPPDFTSMV TLPTGPAKII WEKRNPEQKI 530
    Length:530
    Mass (Da):60,720
    Last modified:July 1, 1989 - v1
    Checksum:i646960BBA0E17979
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti433 – 4331K → N in AAA34546. (PubMed:3046615)Curated
    Sequence conflicti433 – 4331K → N in AAA34547. (PubMed:3046615)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M18109 Genomic DNA. Translation: AAA34379.1.
    M15663 Genomic DNA. Translation: AAA34837.1.
    M21483 Genomic DNA. Translation: AAA34546.1.
    M21484 Genomic DNA. Translation: AAA34547.1.
    U10555 Genomic DNA. Translation: AAB68433.1.
    BK006934 Genomic DNA. Translation: DAA06695.1.
    PIRiA27491.
    RefSeqiNP_011871.1. NM_001179137.1.

    Genome annotation databases

    EnsemblFungiiYHR007C; YHR007C; YHR007C.
    GeneIDi856398.
    KEGGisce:YHR007C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M18109 Genomic DNA. Translation: AAA34379.1 .
    M15663 Genomic DNA. Translation: AAA34837.1 .
    M21483 Genomic DNA. Translation: AAA34546.1 .
    M21484 Genomic DNA. Translation: AAA34547.1 .
    U10555 Genomic DNA. Translation: AAB68433.1 .
    BK006934 Genomic DNA. Translation: DAA06695.1 .
    PIRi A27491.
    RefSeqi NP_011871.1. NM_001179137.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4LXJ X-ray 1.90 A 1-530 [» ]
    ProteinModelPortali P10614.
    SMRi P10614. Positions 6-530.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36434. 238 interactions.
    DIPi DIP-7886N.
    IntActi P10614. 68 interactions.
    MINTi MINT-1325794.
    STRINGi 4932.YHR007C.

    Chemistry

    DrugBanki DB00257. Clotrimazole.
    DB01127. Econazole.
    DB00196. Fluconazole.
    DB01026. Ketoconazole.
    DB01263. Posaconazole.
    DB01153. Sertaconazole.
    DB00251. Terconazole.
    DB01007. Tioconazole.
    DB00582. Voriconazole.

    Proteomic databases

    MaxQBi P10614.
    PaxDbi P10614.
    PeptideAtlasi P10614.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR007C ; YHR007C ; YHR007C .
    GeneIDi 856398.
    KEGGi sce:YHR007C.

    Organism-specific databases

    CYGDi YHR007c.
    SGDi S000001049. ERG11.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00660000095370.
    HOGENOMi HOG000042780.
    KOi K05917.
    OMAi DFTSMVT.
    OrthoDBi EOG7712HB.

    Enzyme and pathway databases

    UniPathwayi UPA00770 ; UER00754 .
    BioCyci MetaCyc:YHR007C-MONOMER.
    YEAST:YHR007C-MONOMER.

    Miscellaneous databases

    NextBioi 981925.
    PROi P10614.

    Gene expression databases

    Genevestigatori P10614.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002403. Cyt_P450_E_grp-IV.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00465. EP450IV.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the P450 lanosterol demethylase gene from Saccharomyces cerevisiae."
      Kalb V.F., Woods C.W., Turi T.G., Dey C.R., Sutter T.R., Loper J.C.
      DNA 6:529-537(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "A single amino acid substitution converts cytochrome P450(14DM) to an inactive form, cytochrome P450SG1: complete primary structures deduced from cloned DNAS."
      Ishida N., Aoyama Y., Hatanaka R., Oyama Y., Imajo S., Ishiguro M., Oshima T., Nakazato H., Noguchi T., Maitra U.S., Mohan V.P., Sprinson D.B., Yoshida Y.
      Biochem. Biophys. Res. Commun. 155:317-323(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Isolation of a cytochrome P-450 structural gene from Saccharomyces cerevisiae."
      Kalb V.F., Loper J.C., Dey C.R., Woods C.W., Sutter T.R.
      Gene 45:237-245(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 444-530.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex."
      Mo C., Bard M.
      J. Lipid Res. 46:1991-1998(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ERG28.
    8. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCP51_YEAST
    AccessioniPrimary (citable) accession number: P10614
    Secondary accession number(s): D3DKV1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 143 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    It is the main target for antifungal compounds of the triazole family like ketoconazole which inhibits by coordinating the iron atom at the sixth ligand position.
    Present with 73200 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3