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Protein

Lanosterol 14-alpha demethylase

Gene

ERG11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.

Catalytic activityi

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.

Cofactori

hemeBy similarity

Pathwayi: zymosterol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Lanosterol 14-alpha demethylase (ERG11)
  2. Delta(14)-sterol reductase (ERG24)
  3. Methylsterol monooxygenase (ERG25)
  4. Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (ERG26)
  5. 3-keto-steroid reductase (ERG27)
  6. no protein annotated in this organism
This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi470Iron (heme axial ligand)By similarity1

GO - Molecular functioni

  • heme binding Source: InterPro
  • iron ion binding Source: InterPro
  • sterol 14-demethylase activity Source: SGD

GO - Biological processi

  • ergosterol biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:YHR007C-MONOMER.
YEAST:YHR007C-MONOMER.
ReactomeiR-SCE-191273. Cholesterol biosynthesis.
R-SCE-211916. Vitamins.
R-SCE-211976. Endogenous sterols.
R-SCE-5365859. RA biosynthesis pathway.
UniPathwayiUPA00770; UER00754.

Names & Taxonomyi

Protein namesi
Recommended name:
Lanosterol 14-alpha demethylase (EC:1.14.13.70)
Alternative name(s):
CYPLI
Cytochrome P450 51
Cytochrome P450-14DM
Cytochrome P450-LIA1
Sterol 14-alpha demethylase
Gene namesi
Name:ERG11
Synonyms:CYP51
Ordered Locus Names:YHR007C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR007C.
SGDiS000001049. ERG11.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 20ExtracellularSequence analysisAdd BLAST20
Transmembranei21 – 41HelicalSequence analysisAdd BLAST21
Topological domaini42 – 530CytoplasmicSequence analysisAdd BLAST489

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000520121 – 530Lanosterol 14-alpha demethylaseAdd BLAST530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki116Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki353Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki454Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei458PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP10614.
PRIDEiP10614.
TopDownProteomicsiP10614.

PTM databases

iPTMnetiP10614.

Interactioni

Subunit structurei

Interacts with ERG28.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ERG25P530453EBI-5127,EBI-6506

Protein-protein interaction databases

BioGridi36434. 241 interactors.
DIPiDIP-7886N.
IntActiP10614. 71 interactors.
MINTiMINT-1325794.

Structurei

Secondary structure

1530
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 23Combined sources15
Helixi27 – 49Combined sources23
Beta strandi53 – 55Combined sources3
Turni64 – 66Combined sources3
Helixi69 – 74Combined sources6
Helixi76 – 87Combined sources12
Beta strandi89 – 95Combined sources7
Beta strandi98 – 103Combined sources6
Helixi105 – 113Combined sources9
Turni117 – 119Combined sources3
Helixi122 – 134Combined sources13
Helixi144 – 155Combined sources12
Helixi160 – 180Combined sources21
Turni182 – 184Combined sources3
Turni186 – 188Combined sources3
Beta strandi190 – 195Combined sources6
Helixi196 – 212Combined sources17
Helixi215 – 220Combined sources6
Helixi225 – 234Combined sources10
Helixi238 – 242Combined sources5
Helixi249 – 274Combined sources26
Beta strandi280 – 282Combined sources3
Helixi283 – 289Combined sources7
Helixi301 – 331Combined sources31
Helixi334 – 347Combined sources14
Helixi349 – 351Combined sources3
Helixi357 – 360Combined sources4
Helixi364 – 376Combined sources13
Beta strandi383 – 387Combined sources5
Beta strandi405 – 408Combined sources4
Helixi410 – 413Combined sources4
Turni417 – 419Combined sources3
Turni421 – 424Combined sources4
Helixi428 – 431Combined sources4
Beta strandi444 – 449Combined sources6
Beta strandi451 – 454Combined sources4
Helixi466 – 468Combined sources3
Helixi473 – 490Combined sources18
Beta strandi491 – 494Combined sources4
Beta strandi507 – 510Combined sources4
Beta strandi518 – 525Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LXJX-ray1.90A1-530[»]
5EQBX-ray2.19A1-530[»]
ProteinModelPortaliP10614.
SMRiP10614.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00800000124060.
HOGENOMiHOG000042780.
InParanoidiP10614.
KOiK05917.
OMAiMSIFIRT.
OrthoDBiEOG092C1MV8.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10614-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATKSIVGE ALEYVNIGLS HFLALPLAQR ISLIIIIPFI YNIVWQLLYS
60 70 80 90 100
LRKDRPPLVF YWIPWVGSAV VYGMKPYEFF EECQKKYGDI FSFVLLGRVM
110 120 130 140 150
TVYLGPKGHE FVFNAKLADV SAEAAYAHLT TPVFGKGVIY DCPNSRLMEQ
160 170 180 190 200
KKFVKGALTK EAFKSYVPLI AEEVYKYFRD SKNFRLNERT TGTIDVMVTQ
210 220 230 240 250
PEMTIFTASR SLLGKEMRAK LDTDFAYLYS DLDKGFTPIN FVFPNLPLEH
260 270 280 290 300
YRKRDHAQKA ISGTYMSLIK ERRKNNDIQD RDLIDSLMKN STYKDGVKMT
310 320 330 340 350
DQEIANLLIG VLMGGQHTSA ATSAWILLHL AERPDVQQEL YEEQMRVLDG
360 370 380 390 400
GKKELTYDLL QEMPLLNQTI KETLRMHHPL HSLFRKVMKD MHVPNTSYVI
410 420 430 440 450
PAGYHVLVSP GYTHLRDEYF PNAHQFNIHR WNKDSASSYS VGEEVDYGFG
460 470 480 490 500
AISKGVSSPY LPFGGGRHRC IGEHFAYCQL GVLMSIFIRT LKWHYPEGKT
510 520 530
VPPPDFTSMV TLPTGPAKII WEKRNPEQKI
Length:530
Mass (Da):60,720
Last modified:July 1, 1989 - v1
Checksum:i646960BBA0E17979
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti433K → N in AAA34546 (PubMed:3046615).Curated1
Sequence conflicti433K → N in AAA34547 (PubMed:3046615).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18109 Genomic DNA. Translation: AAA34379.1.
M15663 Genomic DNA. Translation: AAA34837.1.
M21483 Genomic DNA. Translation: AAA34546.1.
M21484 Genomic DNA. Translation: AAA34547.1.
U10555 Genomic DNA. Translation: AAB68433.1.
BK006934 Genomic DNA. Translation: DAA06695.1.
PIRiA27491.
RefSeqiNP_011871.1. NM_001179137.1.

Genome annotation databases

EnsemblFungiiYHR007C; YHR007C; YHR007C.
GeneIDi856398.
KEGGisce:YHR007C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18109 Genomic DNA. Translation: AAA34379.1.
M15663 Genomic DNA. Translation: AAA34837.1.
M21483 Genomic DNA. Translation: AAA34546.1.
M21484 Genomic DNA. Translation: AAA34547.1.
U10555 Genomic DNA. Translation: AAB68433.1.
BK006934 Genomic DNA. Translation: DAA06695.1.
PIRiA27491.
RefSeqiNP_011871.1. NM_001179137.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LXJX-ray1.90A1-530[»]
5EQBX-ray2.19A1-530[»]
ProteinModelPortaliP10614.
SMRiP10614.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36434. 241 interactors.
DIPiDIP-7886N.
IntActiP10614. 71 interactors.
MINTiMINT-1325794.

PTM databases

iPTMnetiP10614.

Proteomic databases

MaxQBiP10614.
PRIDEiP10614.
TopDownProteomicsiP10614.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR007C; YHR007C; YHR007C.
GeneIDi856398.
KEGGisce:YHR007C.

Organism-specific databases

EuPathDBiFungiDB:YHR007C.
SGDiS000001049. ERG11.

Phylogenomic databases

GeneTreeiENSGT00800000124060.
HOGENOMiHOG000042780.
InParanoidiP10614.
KOiK05917.
OMAiMSIFIRT.
OrthoDBiEOG092C1MV8.

Enzyme and pathway databases

UniPathwayiUPA00770; UER00754.
BioCyciMetaCyc:YHR007C-MONOMER.
YEAST:YHR007C-MONOMER.
ReactomeiR-SCE-191273. Cholesterol biosynthesis.
R-SCE-211916. Vitamins.
R-SCE-211976. Endogenous sterols.
R-SCE-5365859. RA biosynthesis pathway.

Miscellaneous databases

PROiP10614.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP51_YEAST
AccessioniPrimary (citable) accession number: P10614
Secondary accession number(s): D3DKV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 166 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

It is the main target for antifungal compounds of the triazole family like ketoconazole which inhibits by coordinating the iron atom at the sixth ligand position.
Present with 73200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.