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Reviewed, UniProtKB/Swiss-Prot P10613 (CP51_CANAL)

Last modified January 19, 2010. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Lanosterol 14-alpha demethylase
    EC=1.14.13.70
Alternative name(s):
    Cytochrome P450 51
    CYPLI
    Cytochrome P450-LIA1
    Sterol 14-alpha demethylase
    Cytochrome P450-14DM
Gene names
Name: ERG11
Synonyms: CYP51, ERG16
ORF Names: CaO19.922
OrganismCandida albicans (Yeast)
Taxonomic identifier5476 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

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Protein attributes

Sequence length528 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.

Catalytic activity

Obtusifoliol + 3 O2 + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Pathway

Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.

Subcellular location

Membrane Potential. Membrane; Single-pass membrane protein.

Sequence similarities

Belongs to the cytochrome P450 family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 528528Lanosterol 14-alpha demethylase
PRO_0000052003

Sites

Metal binding4701Iron (heme axial ligand) By similarity

Natural variations

Natural variant1051F → L in fluconazole-resistant isolates.
Natural variant2661E → D in strain: fluconazole-resistant isolates.
Natural variant2871K → R in strain: fluconazole-resistant isolates.
Natural variant4501G → E in strain: fluconazole-resistant isolates.
Natural variant4641G → S in strain: fluconazole-resistant isolates.
Natural variant4671R → K in strain: Isolate 13; azole-resistant. Ref.4
Natural variant4881V → I in strain: fluconazole-resistant isolates.

Experimental info

Sequence conflict191V → D in AAB08099. Ref.4

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Sequences

Sequence LengthMass (Da)Tools
P10613-1 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 026948CEE4AF1B88

FASTA52860,675
        10         20         30         40         50         60 
MAIVETVIDG INYFLSLSVT QQISILLGVP FVYNLVWQYL YSLRKDRAPL VFYWIPWFGS 

        70         80         90        100        110        120 
AASYGQQPYE FFESCRQKYG DVFSFMLLGK IMTVYLGPKG HEFVFNAKLS DVSAEDAYKH 

       130        140        150        160        170        180 
LTTPVFGKGV IYDCPNSRLM EQKKFAKFAL TTDSFKRYVP KIREEILNYF VTDESFKLKE 

       190        200        210        220        230        240 
KTHGVANVMK TQPEITIFTA SRSLFGDEMR RIFDRSFAQL YSDLDKGFTP INFVFPNLPL 

       250        260        270        280        290        300 
PHYWRRDAAQ KKISATYMKE IKSRRERGDI DPNRDLIDSL LIHSTYKDGV KMTDQEIANL 

       310        320        330        340        350        360 
LIGILMGGQH TSASTSAWFL LHLGEKPHLQ DVIYQEVVEL LKEKGGDLND LTYEDLQKLP 

       370        380        390        400        410        420 
SVNNTIKETL RMHMPLHSIF RKVTNPLRIP ETNYIVPKGH YVLVSPGYAH TSERYFDNPE 

       430        440        450        460        470        480 
DFDPTRWDTA AAKANSVSFN SSDEVDYGFG KVSKGVSSPY LPFGGGRHRC IGEQFAYVQL 

       490        500        510        520 
GTILTTFVYN LRWTIDGYKV PDPDYSSMVV LPTEPAEIIW EKRETCMF

« Hide

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References

« Hide 'large scale' references
[1]"Nucleotide sequence of cytochrome P450 L1A1 (lanosterol 14 alpha-demethylase) from Candida albicans."
Lai M.H., Kirsch D.R.
Nucleic Acids Res. 17:804-804(1989) [PubMed: 2644625] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SC5314.
[2]"ERG11 mutations in oral strains of Candida albicans isolated in Sardinia."
Orru G., Ciusa M.L., Pilia F., Taccori F., Cosentino S., Pisano M.B., Meloni M., Fadda M.E.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: CA100, CA11, CA36 and CA95.
[3]"The diploid genome sequence of Candida albicans."
Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S.
Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SC5314.
[4]"The presence of an R467K amino acid substitution and loss of allelic variation correlate with an azole-resistant lanosterol 14-alpha demethylase in Candida albicans."
White T.C.
Antimicrob. Agents Chemother. 41:1488-1494(1997) [PubMed: 9210671] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-48 AND 467-528, VARIANT LYS-467.
Strain: SS.
[5]"Modeling cytochrome P450 14-alpha demethylase (Candida albicans) from P450cam."
Boscott P.E., Grant G.H.
J. Mol. Graph. 12:185-192(1994) [PubMed: 7819160] [Abstract]
Cited for: 3D-STRUCTURE MODELING.
[6]"Molecular analysis of cyp51 from fluconazole-resistant Candida albicans strains."
Loffler J., Kelly S.L., Hebart H., Schumacher U., Lass-Florl C., Einsele H.
FEMS Microbiol. Lett. 151:263-268(1997) [PubMed: 9228762] [Abstract]
Cited for: VARIANTS FLUCONAZOLE-RESISTANT.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X13296 Genomic DNA. Translation: CAA31658.1.
EU819548 Genomic DNA. Translation: ACF34636.1.
EU819551 Genomic DNA. Translation: ACF34639.1.
FJ002303 Genomic DNA. Translation: ACH91036.1.
FJ403378 Genomic DNA. Translation: ACJ23064.1.
AACQ01000063 Genomic DNA. Translation: EAK97774.1.
U67192 Genomic DNA. Translation: AAB08099.1.
U67193 Genomic DNA. Translation: AAB08100.1.
PIRO4CK51. S02713.
RefSeqXP_716761.1.

3D structure databases

SMRP10613. Positions 48-523.
ModBaseSearch...

Genome annotation databases

GeneID3641571.
KEGGcal:CaO19.922.

Organism-specific databases

CGDCAL0003627. ERG11.

Phylogenomic databases

OrthoDBEOG99W3XX.
PhylomeDBP10613.

Enzyme and pathway databases

BRENDA1.14.13.70. 1124.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR017973. Cyt_P450_C.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00465. EP450IV.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

DrugBankDB00646. Nystatin.

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Entry information

Entry nameCP51_CANAL
AccessionPrimary (citable) accession number: P10613
Secondary accession number(s): Q5A524, Q92208
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1995
Last modified: January 19, 2010
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

Candida albicans

Candida albicans: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents